LOCUS       sequence-409740-       10413 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  An expression vector of hypoxia-responsive transactivator under 
            control of RTP801 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10413)
  AUTHORS   Masumoto S, Ono A, Ito A, Kawabe Y, Kamihira M
  TITLE     Hypoxia-responsive expression of vascular endothelial growth factor 
            for induction of angiogenesis in artificial three-dimensional 
            tissues.
  JOURNAL   J Biosci Bioeng. 2021 Oct;132(4):399-407. doi: 
            10.1016/j.jbiosc.2021.06.010. Epub 2021 Aug 4.
  PUBMED    34364783
REFERENCE   2  (bases 1 to 10413)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.jbiosc.2021.06.010"; journalName: "J Biosci Bioeng"; 
            date: "2021-10"; volume: "132"; issue: "4"; pages: "399-407"
COMMENT     Sequence Label: pRTP801/TA-ODD
FEATURES             Location/Qualifiers
     source          1..10413
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        25..129
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(130..198,199..990)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(348..367)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1161..1749
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1650..1669
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1903..1920
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2037..2058
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2073..2078,2079..2096,2097..2103)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2111..2127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2116..2138
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2135..2151
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2135..2151
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2200..2321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2211..2230)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2265..2284
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2451..2825)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(2844..2891)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(2939..3268)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2953..3088
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3007..3026)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     3253..3273
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(3282..3710)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3392..3413)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3604..3623
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3756..3980
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     LTR             4012..4192
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4711..5299
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             4817..4828
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5226..5246
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(6148..6167)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             7341..7451
                     /codon_start=1
                     /product="3 tandem repeats of the minimal activation domain
                     of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP48
                     /translation="DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDML"
     polyA_signal    7513..7737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    7862..7979
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8497..8730
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9223..9348
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9395..9575
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(9593..9791)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9618..9638)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9793..10172
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10344..10363
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
       61 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
      121 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
      181 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
      241 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
      301 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
      361 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
      421 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
      481 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
      541 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
      601 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
      661 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
      721 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
      781 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
      841 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
      901 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
      961 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     1021 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     1081 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     1141 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     1201 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     1261 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     1321 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     1381 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     1441 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     1501 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     1561 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     1621 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     1681 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     1741 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     1801 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     1861 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     1921 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     1981 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     2041 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     2101 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     2161 gccaagctct agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg
     2221 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     2281 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc
     2341 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     2401 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc
     2461 tcctcggcca cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc
     2521 cacggctgct cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac
     2581 acgacctccg accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg
     2641 gtgttgtccg gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg
     2701 accacaccgg cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag
     2761 aactcgaccg ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg
     2821 gccatggttt agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat
     2881 taattgtcaa cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt
     2941 tgcaaaagcc taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag
     3001 aggcggcctc ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg
     3061 cggaactggg cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg
     3121 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
     3181 acctggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg
     3241 gggactttcc acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg
     3301 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     3361 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     3421 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     3481 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     3541 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     3601 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     3661 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc
     3721 cctagagccc cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc
     3781 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     3841 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
     3901 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     3961 ctggcaacta gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctgctagaga
     4021 ttttccacac tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac
     4081 gggcacacac tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc
     4141 ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagt
     4201 ccggatgcag ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac ctccactcta
     4261 atacttctct ctccgggtca tccatcccat gcaggctcac agggtgtaac aagcgggtgt
     4321 tctctccttc attggcttct tctaccttct cttgctcaac tggtactagc ttgtagcacc
     4381 atccaaaggt cagtggatat ctgatccctg gccctggtgt gtagttctgc caatcaggga
     4441 agtagccttg tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa
     4501 ttagcccttc cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt
     4561 aagtcattgg tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt
     4621 gtgcttctag ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc
     4681 catgtttttc taggtctcga cggtatcgat gcggggaggc ggcccaaagg gagatccgac
     4741 tcgtctgagg gcgaaggcga agacgcggaa gaggccgcag agccggcagc aggccgcggg
     4801 aaggaaggtc cgctggattg agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa
     4861 tccaggtggc aacacaggcg agcagccaag gaaaggacga tgatttcccc gacaacacca
     4921 cggaattgtc agtgcccaac agccgagccc ctgtccagca gcgggcaagg caggcggcga
     4981 tgagttccgc cgtggcaata gggaggggga aagcgaaagt cccggaaagg agctgacagg
     5041 tggtggcaat gccccaacca gtgggggttg cgtcagcaaa cacagtgcac accacgccac
     5101 gttgcctgac aacgggccac aactcctcat aaagagacag caaccaggat ttatacaagg
     5161 aggagaaaat gaaagccata cgggaagcaa tagcatgata caaaggcatt aaagcagcgt
     5221 atccacatag cgtaaaagga gcaacatagt taagaatacc agtcaatctt tcacaaattt
     5281 tgtaatccag aggttgatta tcgataagct tgatatcgaa ttcgataagg taccactagt
     5341 agctttagga tccaagacgc tgggggcaac cattttcctt gcccgccgcc ccctcacgct
     5401 tccctgcctc tcctcctagc ctggtcacgg gctgtcccct cctccagcaa tgcaacccta
     5461 taataaacaa gtctttcctt gatcctcccc tgccgcgagc gccctcgggg accttggcag
     5521 ctgcagccgc cgcggatcct ttccagaaag ggggcgtggc ggtgggtcgg ggttcgactg
     5581 cgagctttct ggggctcaat ggaggcgggg cccggccgct gtcaccgggc aggagagaac
     5641 gttgcttacg tgcgcccgga gtccattggc caaggcgggc cacactcccg ggtctggatt
     5701 gggtcgtggc gcagagaagg cgtggcctcg ccgcgctagt ccttataggc tgctccgcgc
     5761 tggtgctagg gcgcagcagg ccaaggggga ggtgcgagcg tggacctggg acgggtctgg
     5821 gcggctctcg gtggttggca cgggttcgca cacccattca agcggcagga cgcacttgtc
     5881 ttagcagttc tcgctgaccg cgctagctgg tgagtgtccc ttctgtgtgt gggtcctaga
     5941 gctcgcggtc tggtctggtc tggtccccag actgacgcct ggtcggtccc cctcttgtct
     6001 tacagcggct tctacgctcc ggcactctga gttcatcagc aaacgccctg gcgtctgtcc
     6061 tcaccctcga gaccatgtct aggttagata aaagtaaagt gattaacagc gcattagagc
     6121 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
     6181 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
     6241 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
     6301 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
     6361 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
     6421 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
     6481 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
     6541 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
     6601 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgtg
     6661 gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cggatccgcc ctcacactgc
     6721 ttgctccagc agctggagac acgatcatat ccctggactt tggctccaat gacacagaga
     6781 cagatgacca gcagttggag gaggttcccc tctataacga cgtcatgctg ccttctccca
     6841 atgagaagct gcagaacatc aatctggcca tgtctccgct gccaacagcc gaaactccta
     6901 aacccctcag aagcagtgct gatcctgcac tgaaccagga agtagccctc aaactggaac
     6961 caaatccgga aagcctggag ttgtccttca ctatgcctca gattcaggat cagaccccat
     7021 caccttccga tgggtctacc aggcaaagtt ccccagaacc caatagcccc tcagagtact
     7081 gcttctatgt ggatagtgac atggtgaacg agttcaagct ggaactggtg gagaaactct
     7141 ttgctgagga taccgaagcc aagaacccgt tctcaacgca agacaccgat ctggatcttg
     7201 agatgcttgc gccatacatt cccatggatg acgacttcca actgcggtct tttgaccagc
     7261 tgtctccctt ggagagcagc tcagcaagcc ctgaaagcgc cagtcctcaa tccactgtca
     7321 ccgtgtttca gcagacgcgt gacgcccttg acgattttga cttagacatg ctcccagccg
     7381 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     7441 ttgacatgct ccccgggtaa ctcagattat caagcttgat aatctagaaa gctagccgct
     7501 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7561 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7621 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7681 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7741 ctgaggcgac tagagcggcc gccaccgcgg gaggcgccaa aacccggcgc ggaggccaga
     7801 tctggagccg acacgggtta attaaccaaa ctggatctct gctgtccctg taataaaccc
     7861 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
     7921 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
     7981 tgtggatgaa tactgccatt tgtctgcaga attggcgcac gcagtgccga tccgttcact
     8041 aatcgaatgg atctgtctct gtctctctct ccaccttctt cttctattcc ttcgggcctg
     8101 tcgggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     8161 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     8221 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     8281 cttgcccatt tatctaattc ttgttcattc ttttcttgct ggttttgcga ttcttcaatt
     8341 aaggagtgta ttaagcttgt gtaattgtta atttctctgt cccactccat ccaggtcgtg
     8401 tgattccaaa tctgttccag agatttatta ctccaactag cattccaagg cacagcagtg
     8461 gtgcaaatga gttttccaga gcaaccccaa atccccagga gctgttgatc ctttaggtat
     8521 ctttccacag ccaggattct tgcctggagc tgcttgatgc cccagactgt gagttgcaac
     8581 agatgctgtt gcgcctcaat agccctcagc aaattgttct gctgctgcac tataccagac
     8641 aataattgtc tggcctgtac cgtcagcgtc attgacgctg cgcccatagt gcttcctgct
     8701 gctcccaaga acccaaggaa caaagctcct attcccactg ctcttttttc tctctgcacc
     8761 actcttctct ttgccttggt gggtgctact cctaatggtt caatttttac tactttatat
     8821 ttatataatt cacttctcca attgtccctc atatctcctc ctccaggtct gaagatcagc
     8881 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc
     8941 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt
     9001 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat
     9061 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc
     9121 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg
     9181 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc
     9241 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc
     9301 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt
     9361 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa
     9421 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga
     9481 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc
     9541 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaacg cgctgcttat
     9601 atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg agttgttacg
     9661 acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga cgtcaatggg
     9721 gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga tgtactgcca
     9781 aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa
     9841 gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc
     9901 aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg
     9961 taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata
    10021 cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac
    10081 cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga
    10141 ttactattaa taactagtca ataatcaatg tcaacgcgta tatctggccc gtacatcgcg
    10201 aagcagcgca aaacgcctaa ccctaagcag attcttcatg caattgtcgg tcaagccttg
    10261 ccttgttgta gcttaaattt tgctcgcgca ctactcagcg acctccaaca cacaagcagg
    10321 gagcagatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatag
    10381 gggatcggga gatctcccga tccgtcgacg tca
//
