LOCUS       sequence-409743-        8307 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression plasmid for SARS-CoV-2 BA.1 variant spike .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Dadonaite B, Crawford KHD, Radford CE, Farrell AG, Yu TC, Hannon WW,
            Zhou P, Andrabi R, Burton DR, Liu L, Ho DD, Chu HY, Neher RA, Bloom 
            JD
  TITLE     A pseudovirus system enables deep mutational scanning of the full 
            SARS-CoV-2 spike.
  JOURNAL   Cell. 2023 Mar 16;186(6):1263-1278.e20. doi: 
            10.1016/j.cell.2023.02.001. Epub 2023 Feb 13.
  PUBMED    36868218
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2023.02.001"; journalName: "Cell"; date: 
            "2023-03-16- 16"; volume: "186"; issue: "6"; pages: "1263-1278.e20"
COMMENT     Sequence Label: HDM_omicron_B11529_IDTDNA
FEATURES             Location/Qualifiers
     source          1..8307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        82..461
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        462..665
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     616..636
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     662..686
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          779..1254
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     primer_bind     complement(5077..5093)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5078..5094)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    5187..5581
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     primer_bind     complement(5915..5933)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6001..6105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6106..6174,6175..6966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6324..6343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7137..7725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7626..7645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 agcttggccc attgcatacg ttgtatccat atcataatat gtacatttat attggctcat
       61 gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag taatcaatta
      121 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      181 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      241 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      301 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      361 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      421 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      481 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      541 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      601 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      661 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
      721 atagaagaca ccgggaccga tccagcctcc cctcgaagct gatcctgaga acttcagggt
      781 gagtctatgg gacccttgat gttttctttc cccttctttt ctatggttaa gttcatgtca
      841 taggaagggg agaagtaaca gggtacacat attgaccaaa tcagggtaat tttgcatttg
      901 taattttaaa aaatgctttc ttcttttaat atactttttt gtttatctta tttctaatac
      961 tttccctaat ctctttcttt cagggcaata atgatacaat gtatcatgcc tctttgcacc
     1021 attctaaaga ataacagtga taatttctgg gttaaggcaa tagcaatatt tctgcatata
     1081 aatatttctg catataaatt gtaactgatg taagaggttt catattgcta atagcagcta
     1141 caatccagct accattctgc ttttatttta tggttgggat aaggctggat tattctgagt
     1201 ccaagctagg cccttttgct aatcatgttc atacctctta tcttcctccc acagctcctg
     1261 ggcaacgtgc tggtctgtgt gctggcccat cactttggca aagaattccg ccaccatgtt
     1321 cgtgtttctc gtactgctgc cactcgtatc cagtcagtgt gtgaacctta cgacccgcac
     1381 acagcttccc ccggcataca ccaatagctt taccaggggg gtttattacc cggataaggt
     1441 atttaggtca tcagttttgc attcaacgca agacttgttt ctgccgttct tttctaacgt
     1501 aacgtggttc cacgtgatta gtggcacaaa cggtacgaaa cgatttgaca accctgtgct
     1561 tcctttcaat gacggagttt atttcgcatc tatcgagaaa tccaacatca tccggggttg
     1621 gatatttgga actactttgg attcaaagac gcagtccctc ctgattgtca ataatgcgac
     1681 aaatgtcgtt ataaaagttt gcgaatttca attctgtaat gacccctttc tggaccataa
     1741 aaataacaag tcatggatgg agtcagaatt tcgggtgtat tcttctgcta ataactgtac
     1801 cttcgagtac gtatcacaac cgttcctgat ggacctggag ggcaagcaag gaaattttaa
     1861 gaacttgcga gaatttgtgt ttaagaatat tgatgggtat ttcaagatat atagcaaaca
     1921 taccccgatt atcgttaggg aaccggagga tctgccgcaa gggtttagtg ctctggaacc
     1981 gcttgttgat ttgccaattg gaataaatat tactagattc cagacgctcc ttgcactcca
     2041 tagaagttac ttgacaccgg gcgactcatc ctctggttgg accgcaggag ccgcggctta
     2101 ttatgtgggc tatctgcagc cgcggacttt ccttttgaaa tacaatgaga atggcaccat
     2161 tacagacgcc gtcgattgtg cgcttgatcc actcagcgag acaaaatgca ctctcaaatc
     2221 cttcactgtt gagaagggca tctatcaaac atcaaacttt cgcgttcaac cgacggaatc
     2281 catcgtacga tttccgaata tcaccaatct ctgccctttt gatgaagtat ttaatgcgac
     2341 gcggtttgct agcgtgtacg cctggaatcg aaagcgaatt agcaactgcg ttgcagacta
     2401 ttcagtgctg tacaatctcg caccgttctt tacgtttaag tgttatggcg ttagtccgac
     2461 taaattgaac gatctgtgtt ttacaaatgt ttacgccgat tccttcgtta taaggggcga
     2521 tgaagtcagg cagatcgctc cgggtcagac ggggaatatt gcagattaca attacaaact
     2581 cccagacgac tttactgggt gcgttatagc ttggaacagt aacaaactcg acagcaaagt
     2641 atcaggtaac tataattact tgtatcggct gtttcggaag tcaaacttga agccatttga
     2701 gcgagacatc agcacagaga tataccaagc aggtaataaa ccctgtaatg gcgtcgcagg
     2761 cttcaattgc tattttccgt tgagaagcta ctcctttcgg cctacgtatg gggtaggaca
     2821 tcaaccctac cgagtggtgg tactcagctt cgagttgctg catgctcctg cgacagtttg
     2881 tgggccgaag aagtccacca acctcgtgaa aaataagtgc gtgaatttta acttcaacgg
     2941 actgaagggt actggtgttt tgacggaatc taataagaag tttctgccat ttcaacaatt
     3001 cggccgggac atcgctgaca ctaccgacgc tgtgagggat ccccaaacac ttgaaatttt
     3061 ggatatcaca ccgtgctcat ttggaggcgt aagcgtaata acacccggga ccaatacctc
     3121 aaaccaagta gcagtcttgt accagggcgt taattgcacc gaagtcccgg tggctatcca
     3181 tgctgaccag ctgaccccca catggagagt atacagcaca ggttctaacg tttttcagac
     3241 aagggctggc tgcctgatag gggcggagta cgtcaataat tcttacgagt gcgatattcc
     3301 catcggtgcc ggcatttgtg catcctacca gacacagacc aaatctcacc gacgcgccag
     3361 gtcagttgca tcacaatcca ttatcgcata cactatgtcc ctgggagctg agaattcagt
     3421 cgcttactcc aataacagca tagcgatacc taccaatttt accatttctg tcactactga
     3481 gatattgccg gtgtcaatga ccaaaacaag cgtagattgt actatgtaca tctgtgggga
     3541 ctcaacggag tgctccaatc tcctgttgca atacgggtcc ttttgcactc aactcaagcg
     3601 cgcgcttaca ggtattgccg tagaacagga caagaatacg caggaagtgt tcgcacaagt
     3661 aaaacagatt tacaagaccc cacctatcaa gtattttggt ggtttcaatt tctcccaaat
     3721 cctgccagat cctagtaagc cgagtaagcg gtcctttata gaggatcttt tgttcaataa
     3781 ggtcacactt gccgacgccg gttttataaa acaatacggt gactgtctcg gagacattgc
     3841 agcccgagac ttgatatgtg cccaaaagtt caagggactc acagtattgc cacctctttt
     3901 gactgacgag atgatagccc agtacacatc tgcactcttg gctggaacta tcacctccgg
     3961 gtggaccttt ggagcaggag cggctttgca gataccgttc gcgatgcaga tggcctatcg
     4021 ctttaatggc ataggtgtaa cgcaaaatgt cctctatgag aaccaaaaac tcattgcgaa
     4081 tcagtttaat tccgcgattg gcaaaattca ggatagcctg tctagcacag ccagcgcttt
     4141 gggaaaattg caggatgtgg taaaccacaa cgcgcaggct ctcaatacgc ttgtaaaaca
     4201 gttgagctct aaatttggag ctattagttc cgtcctgaac gacatcttct cccgcttgga
     4261 taaagtagag gctgaggttc aaatagatag gctcatcacc ggcagactcc agtcattgca
     4321 gacttacgtg acccaacaac tgatccgggc tgcagagatc agggcatcag cgaacctcgc
     4381 cgcaactaaa atgagtgagt gcgtgctggg gcaatctaag cgggtagact tttgcggaaa
     4441 aggatatcac ttgatgagct tcccgcagtc tgcaccccac ggggttgtgt ttttgcacgt
     4501 tacatacgtg ccggctcagg agaaaaactt cacgactgcg ccagcaatct gccatgatgg
     4561 taaggctcat tttccgcgcg agggggtatt tgtcagcaat gggacacact ggtttgtgac
     4621 tcaaaggaac ttttatgagc ctcaaataat tacgaccgat aacacttttg tctcaggtaa
     4681 ttgcgatgta gtcatcggga ttgtgaacaa cacggtttat gaccccttgc aaccagagct
     4741 tgactccttt aaggaggagc tcgataagta cttcaaaaat cacactagcc ctgacgtaga
     4801 tctcggagac atatcaggca tcaatgctag tgtagttaat atacaaaaag agatagaccg
     4861 cctgaacgaa gtggccaaga atctcaacga atcccttata gacctccaag aacttgggaa
     4921 gtacgaacag tacatcaagt ggccgtggta catatggctt ggctttattg caggattgat
     4981 tgccatagtg atggtcacca taatgctctg ctgtatgacc agctgttgca gttgtttgaa
     5041 aggctgctgt tcttgtggga gctgatgaaa gcttatcgat accgtcgacc tcgagggccc
     5101 agatctaatt caccccacca gtgcaggctg cctatcagaa agtggtggct ggtgtggcta
     5161 atgccctggc ccacaagtat cactaagctc gctttcttgc tgtccaattt ctattaaagg
     5221 ttcctttgtt ccctaagtcc aactactaaa ctgggggata ttatgaaggg ccttgagcat
     5281 ctggattctg cctaataaaa aacatttatt ttcattgcaa tgatgtattt aaattatttc
     5341 tgaatatttt actaaaaagg gaatgtggga ggtcagtgca tttaaaacat aaagaaatga
     5401 agagctagtt caaaccttgg gaaaatacac tatatcttaa actccatgaa agaaggtgag
     5461 gctgcaaaca gctaatgcac attggcaaca gcccctgatg cctatgcctt attcatccct
     5521 cagaaaagga ttcaagtaga ggcttgattt ggaggttaaa gttttgctat gctgtatttt
     5581 acattactta ttgttttagc tgtcctcatg aatgtctttt cactacccat ttgcttatcc
     5641 tgcatctctc agccttgact ccactcagtt ctcttgctta gagataccac ctttcccctg
     5701 aagtgttcct tccatgtttt acggcgagat ggtttctcct cgcctggcca ctcagcctta
     5761 gttgtctctg ttgtcttata gaggtctact tgaagaagga aaaacagggg gcatggtttg
     5821 actgtcctgt gagcccttct tccctgcctc ccccactcac agtgacccgg aatccctcga
     5881 catggcagtc tagatcattc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5941 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     6001 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     6061 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     6121 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6181 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6241 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6301 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     6361 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6421 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6481 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6541 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6601 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     6661 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6721 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6781 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6841 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6901 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6961 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     7021 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     7081 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     7141 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7201 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7261 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7321 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7381 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7441 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7501 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7561 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7621 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7681 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgga
     7741 gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt
     7801 ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa
     7861 tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg
     7921 cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg
     7981 ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc aatgatcgaa
     8041 gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc
     8101 tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc
     8161 ataatgggga aggccatcca gcctcgcgtc ggggagcttt ttgcaaaagc ctaggcctcc
     8221 aaaaaagcct cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc
     8281 ataaataaaa aaaattagtc agccatg
//
