LOCUS       sequence-409768-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for AFD neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH63 - [AFDp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1872..1888)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1872..1888)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1885..1907)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2060..2648)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2140..2159)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2765..2789)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2889..3680,3681..3749))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3512..3531
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3750..3854)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3953..3975
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3967..3984
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3968..3984
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4608..4641)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tcaaaaatga ttacgccaag ctgtaagttt aaacatgatc ttactaacta actattctca
       61 tttaaatttt cagagcttaa aaatggctga aatcactcac aacgatggat acgctaatgc
      121 ctgtgaatag agttctcgta aatcgacaat ggagttttca aaaaatacat aacgatgagt
      181 aatccttgtt atattttcca ttagagtaaa ttgatataaa gaaaacagag ttatatctga
      241 agttgagaag cttaaaagat ttatgactac cgtaagaata tccggatgag ctgatacaga
      301 tggctcatga agtgacgcaa cgggtttcat ttcgtacatt tttatcaact catattcgat
      361 ccacggtaac tatcgtgaga agctgtaaaa ggattttcga ttctaccttt tccacataaa
      421 aagagaccaa aaatggggcc cgtttcaaag ggagaggccg ttataaaaga atttatgcgt
      481 ttcaaggtcc acatggaggg atccatgaac ggacacgagt tcgagatcga gggagaggga
      541 gagggacgtc catacgaggg aacccaaacc gccaaggtga gttttattat cgattttcgg
      601 gattttctac ttgtaattac cttttgtttt taataagttg cttttttcca gctcaaggtc
      661 accaagggag gaccactccc attctcctgg gacatcctct ccccacaatt catgtacgga
      721 tcccgtgcct tcaccaagca cccagccgac atcccagact actacaagca atccttccca
      781 gagggattca agtgggagcg tgtcatgaac ttcgaggacg gaggagccgt caccgtcacc
      841 caaggtaggt tttcagtttc aatttttgtt gcaactacct caactctatg ttttcagaca
      901 cctccctcga ggacggaacc ctcatctaca aggtcaagct ccgtggaacc aacttcccac
      961 cagacggacc agtcatgcaa aagaagacca tgggatggga ggcctccacc gagcgactct
     1021 acccagagga cggagtcctc aagggagaca tcaaggtaaa tttttcaagc ttctaaattc
     1081 caaaataaac cattttaatt tcttaattcc agatggccct ccggctcaag gacggaggac
     1141 gttacctcgc cgacttcaag accacctaca aggccaagaa gccagtccaa atgccaggag
     1201 cctacaacgt cgaccgtaag ctcgacatca cctcccacaa cgaggactac accgtcgtcg
     1261 agcaatacga gcgttccgag ggacgtcact ccaccggagg aatggacgag ctctacaagc
     1321 ccgggtgatg gtctctgagc tatgcaaaat cctttcaagc attcccttct tctctatcac
     1381 tcttctttct ttttgtcaaa aaattctctc gctaatttat ttgctttttt aatgttatta
     1441 ttttatgact ttttatagtc actgaaaagt ttgcatctga gtgaagtgaa tgctatcaaa
     1501 atgtgattct gatatccagg aaacggagaa tctgtgcggc aatgtcttat catttgaaac
     1561 gcgccgtatc gatgctgaca tcatgcggct cccgtgttag tcgagaagat gggtacgggc
     1621 ctttattcat ccaacactct acggcttctt caagagctag tagggcaccc tgtagttgga
     1681 aagggattat ttcgtatggc gagcccatac cgtctttctt gcggaagctt aacacgatag
     1741 gaagttggat agtttcgaac gatggttcca tacggaacgc tgtctcaagg atgagtctga
     1801 cggagtgtaa ctcgaaggct aggtggaggc tcagtgatga taagtctgcg atggtggatg
     1861 catgtgtcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcag agggcacaat
     1921 cctattccgc gctatccgac aatctccaag acattaggtg gagttcagtt cggcgtatgg
     1981 catatgtcgc tggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2041 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2101 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2161 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2221 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2281 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2341 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2401 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     2461 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     2521 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     2581 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     2641 gatctcaaga agatcctttg atcttttcta cggggtctga cgctctattc aacaaagccg
     2701 ccgtcccgtc aagtcagcgt aaatgggtag ggggcttcaa atcgtcctcg tgataccaat
     2761 tcggagcctg cttttttgta caaacttgtt gataatggca attcaaggat cttcacctag
     2821 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     2881 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2941 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     3001 tctggcccca gtgctgcaat gataccgcga gagccacgct caccggctcc agatttatca
     3061 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     3121 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     3181 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     3241 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     3301 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     3361 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     3421 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     3481 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     3541 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     3601 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     3661 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     3721 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     3781 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     3841 ataggggttc cgcgcacatt tccccgaaaa gtgccagata cctgaaacaa aacccatcgt
     3901 acggccaagg aagtctccaa taactgtgat ccaccacaag cgccagggtt ttcccagtca
     3961 cgacgttgta aaacgacggc cagtcatgca taatccgcac gcatctggaa taaggaagtg
     4021 ccattccgcc tgacctaagt gcttttcgag atcgccaaac cggagaacga tcaggagaat
     4081 gatgagtcgc cacaggagtc ggcaagatac gtgcgttata gatttgctcc aaactttttg
     4141 aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag gagacatccc aatccatcat
     4201 ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc tgaagtgttt tgactttgaa
     4261 ttcggattct gtattccgaa ttcacgaaac aactgtgaac atatctatga gttccctcaa
     4321 ctctctcaac aactcagtga gtcattattc taaaaagtac aattcaaaag actaatctct
     4381 ttttcagtgg acgacatgat caacaatcca aacgagactc gttctgacag cttctatttc
     4441 gtcgataaca aactcgtcat gcacaacaaa gccgactact catatgatgc ataaatcttc
     4501 attgttgagt ttatcttgtt gatttttgaa taaattatca actctttact ttttaatggg
     4561 ttatgaaata aataaacatt gaaaactgat aaacaacgtt catctctata acttcgtata
     4621 gcatacatta tacgaagtta tgata
//
