LOCUS       sequence-409771-        4645 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mScarlet BioPart for AWA neurons. (1) Mark neurons or (2) 
            cell-specific expression of transgenes (N or C-terminal fusions, or 
            untagged). Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4645)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH79 - [AWAp | wrmScarlet | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4645
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(359..375)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(359..375)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(372..394)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(547..1135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(627..646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(1252..1276)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(1376..2167,2168..2236))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1999..2018
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2237..2341)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2440..2462
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2454..2471
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2455..2471
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3095..3128)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 atccaggaaa cggagaatct gtgcggcaat gtcttatcat ttgaaacgcg ccgtatcgat
       61 gctgacatca tgcggctccc gtgttagtcg agaagatggg tacgggcctt tattcatcca
      121 acactctacg gcttcttcaa gagctagtag ggcaccctgt agttggaaag ggattatttc
      181 gtatggcgag cccataccgt ctttcttgcg gaagcttaac acgataggaa gttggatagt
      241 ttcgaacgat ggttccatac ggaacgctgt ctcaaggatg agtctgacgg agtgtaactc
      301 gaaggctagg tggaggctca gtgatgataa gtctgcgatg gtggatgcat gtgtcatggt
      361 catagctgtt tcctgtgtga aattgttatc cgctcagagg gcacaatcct attccgcgct
      421 atccgacaat ctccaagaca ttaggtggag ttcagttcgg cgtatggcat atgtcgctgg
      481 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      541 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      601 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      661 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      721 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      781 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      841 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      901 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      961 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     1021 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     1081 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1141 tcctttgatc ttttctacgg ggtctgacgc tctattcaac aaagccgccg tcccgtcaag
     1201 tcagcgtaaa tgggtagggg gcttcaaatc gtcctcgtga taccaattcg gagcctgctt
     1261 ttttgtacaa acttgttgat aatggcaatt caaggatctt cacctagatc cttttaaatt
     1321 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     1381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     1441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     1501 ctgcaatgat accgcgagag ccacgctcac cggctccaga tttatcagca ataaaccagc
     1561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     1621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     1681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     1741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     1801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     1861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     1921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     1981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     2041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     2101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     2161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     2221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     2281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     2341 gcacatttcc ccgaaaagtg ccagatacct gaaacaaaac ccatcgtacg gccaaggaag
     2401 tctccaataa ctgtgatcca ccacaagcgc cagggttttc ccagtcacga cgttgtaaaa
     2461 cgacggccag tcatgcataa tccgcacgca tctggaataa ggaagtgcca ttccgcctga
     2521 cctaagtgct tttcgagatc gccaaaccgg agaacgatca ggagaatgat gagtcgccac
     2581 aggagtcggc aagatacgtg cgttatagat ttgctccaaa ctttttgaaa ctcaaaacgg
     2641 tcggagcaac tgtggaattc aaagtaggag acatcccaat ccatcatttc cgaatgatcg
     2701 aacgtcactt cttcaaagat cgccttctga agtgttttga ctttgaattc ggattctgta
     2761 ttccgaattc acgaaacaac tgtgaacata tctatgagtt ccctcaactc tctcaacaac
     2821 tcagtgagtc attattctaa aaagtacaat tcaaaagact aatctctttt tcagtggacg
     2881 acatgatcaa caatccaaac gagactcgtt ctgacagctt ctatttcgtc gataacaaac
     2941 tcgtcatgca caacaaagcc gactactcat atgatgcata aatcttcatt gttgagttta
     3001 tcttgttgat ttttgaataa attatcaact ctttactttt taatgggtta tgaaataaat
     3061 aaacattgaa aactgataaa caacgttcat ctctataact tcgtatagca tacattatac
     3121 gaagttatga tatcaaaaat gattacgcca agctgtaagt ttaaacatga tcttactaac
     3181 taactattct catttaaatt ttcagagctt aaaaatggct gaaatcactc acaacgatgg
     3241 atacgctaat gccaaatgta acatctttca ttgttcataa aaatatacgt gttcgatagt
     3301 tgagtttcat gagtcaacaa ttagaactaa gtcagaatgt tttgaaattt ttgaaataaa
     3361 aagctacgat tcaataactc ctatcttgat tttatgattt tgacagaacc gtcatcattc
     3421 tcattactca ttttattctt ttcgtgtgga aatttcatga aaaacaacta cagaaaaaac
     3481 tacaaatttt gttcttaaag taacacaata aaagggaatt cttagtggga ttttagtcac
     3541 acttccgaga aaaagagacc aaaaatgggg cccgtttcaa agggagaggc cgttataaaa
     3601 gaatttatgc gtttcaaggt ccacatggag ggatccatga acggacacga gttcgagatc
     3661 gagggagagg gagagggacg tccatacgag ggaacccaaa ccgccaaggt gagttttatt
     3721 atcgattttc gggattttct acttgtaatt accttttgtt tttaataagt tgcttttttc
     3781 cagctcaagg tcaccaaggg aggaccactc ccattctcct gggacatcct ctccccacaa
     3841 ttcatgtacg gatcccgtgc cttcaccaag cacccagccg acatcccaga ctactacaag
     3901 caatccttcc cagagggatt caagtgggag cgtgtcatga acttcgagga cggaggagcc
     3961 gtcaccgtca cccaaggtag gttttcagtt tcaatttttg ttgcaactac ctcaactcta
     4021 tgttttcaga cacctccctc gaggacggaa ccctcatcta caaggtcaag ctccgtggaa
     4081 ccaacttccc accagacgga ccagtcatgc aaaagaagac catgggatgg gaggcctcca
     4141 ccgagcgact ctacccagag gacggagtcc tcaagggaga catcaaggta aatttttcaa
     4201 gcttctaaat tccaaaataa accattttaa tttcttaatt ccagatggcc ctccggctca
     4261 aggacggagg acgttacctc gccgacttca agaccaccta caaggccaag aagccagtcc
     4321 aaatgccagg agcctacaac gtcgaccgta agctcgacat cacctcccac aacgaggact
     4381 acaccgtcgt cgagcaatac gagcgttccg agggacgtca ctccaccgga ggaatggacg
     4441 agctctacaa gcccgggtga tggtctctga gctatgcaaa atcctttcaa gcattccctt
     4501 cttctctatc actcttcttt ctttttgtca aaaaattctc tcgctaattt atttgctttt
     4561 ttaatgttat tattttatga ctttttatag tcactgaaaa gtttgcatct gagtgaagtg
     4621 aatgctatca aaatgtgatt ctgat
//
