LOCUS       sequence-409773-       12021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BLINCAR plasmid for gene deletion, URA3 target.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12021)
  AUTHORS   Reichard WD, Smith SE, Robertson JB
  TITLE     BLINCAR: a reusable bioluminescent and Cas9-based genetic toolset 
            for repeatedly modifying wild-type Scheffersomyces stipitis.
  JOURNAL   mSphere. 2023 Aug 24;8(4):e0022423. doi: 10.1128/msphere.00224-23. 
            Epub 2023 Jun 22.
  PUBMED    37345937
REFERENCE   2  (bases 1 to 12021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "mSphere."; 
            date: "2023-08-24"; volume: "8(4):e0022423. doi"; pages: " 
            10.1128/msphere.00224-23. Epub 2023 Jun 22"
COMMENT     Sequence Label: pWR95(URA3)
FEATURES             Location/Qualifiers
     source          1..12021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2059..2075)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3413..4222
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             5418..7046
                     /codon_start=1
                     /product="green-emitting variant of click beetle 
                     luciferase"
                     /label=CBG99luc
                     /note="human codon-optimized"
                     /translation="MVKREKNVIYGPEPLHPLEDLTAGEMLFRALRKHSHLPQALVDVV
                     GDESLSYKEFFEATVLLAQSLHNCGYKMNDVVSICAENNTRFFIPVIAAWYIGMIVAPV
                     NESYIPDELCKVMGISKPQIVFTTKNILNKVLEVQSRTNFIKRIIILDTVENIHGCESL
                     PNFISRYSDGNIANFKPLHFDPVEQVAAILCSSGTTGLPKGVMQTHQNICVRLIHALDP
                     RVGTQLIPGVTVLVYLPFFHAFGFSITLGYFMVGLRVIMFRRFDQEAFLKAIQDYEVRS
                     VINVPSVILFLSKSPLVDKYDLSSLRELCCGAAPLAKEVAEVAAKRLNLPGIRCGFGLT
                     ESTSANIHSLGDEFKSGSLGRVTPLMAAKIADRETGKALGPNQVGELCIKGPMVSKGYV
                     NNVEATKEAIDDDGWLHSGDFGYYDEDEHFYVVDRYKELIKYKGSQVAPAELEEILLKN
                     PCIRDVAVVGIPDLEAGELPSAFVVKQPGKEITAKEVYDYLAERVSHTKYLRGGVRFVD
                     SIPRNVTGKITRKELLKQLLEKAGG"
     RBS             6368..6376
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(7497..7513)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(10266..10854)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10346..10365)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11025..11816,11817..11885))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11648..11667
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11886..11990)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccaatgatac cggtgcgatc gtgggcccta gtacttccct cgaggatatc aaagaaagaa
       61 gaagccatta ctgtcagtga aatggccgtt tggtttggta gagattttaa ggctcaattg
      121 tcgacggtcg ttctattcag attaatcaag aagactgaca gcaagattac tcagtcttgg
      181 ggtaagatca taaagattat tttgacattg tttgagaatt gtttgataaa tcctaacttg
      241 ttcagcgaat ttcaaaagaa aatcaagctt agtccattac ctaaagttaa gccaagatat
      301 atcatcaaga gggttaagcc attgaataat tctggtatct tgtcgacgtt ctcgtcattc
      361 ttgaagggct attctgatga tcctcctgag cctacagatc atgaaattga gtccaccttg
      421 tccactatcg actgtgtgaa atccattaac gtaccagcca tcttcgaggt tgtatccaag
      481 ggaccacctg atgacttaaa atattttgtt aaactcattt tgaattcgtt gccagagtat
      541 agtaaggata caaaaagata ttatgagaca gaaattctct ttttgttgga aattgcggtc
      601 tgtttctgtt tgtcattaga tgatattgaa ttgattactg aagtctcgga caagctcaac
      661 ccaccagaaa tatccaagaa gggccaactt agattaattg catacaagtt acttttggcc
      721 agacgtggta attcgaagat taacgtttct gcttccatta agactttagc tgagtttgat
      781 aaggaaatca ttaacaagca aggcggtcaa ttacttcaac cattgttatc cttgattgat
      841 gatgacgctt ggtgttgtaa ggaattgtta aatgacaatg aatactggaa agtattaagg
      901 ttctttgctt ctatccaaat ttatgcatct gatattctcg aattcatgtc tggaattgtc
      961 aagaatgctc ctcaggacat aacgccatct aattatattg catttttggg attacttgat
     1021 gagatttcct ccttaggagc tattggctct cagtacgaac aagagaatga aaagctcggt
     1081 tcgaatcaag acataactta cttcaaggac ttggtggaac tttccaagaa atcaatatca
     1141 ttgacttctg aattatctgc tgtgataaag agaccagaat tcaagggcat ggaattgacc
     1201 tacacattga ttcaagcatt ggcacaccag tgtttcaatc catgttgcga tgttcgttct
     1261 tttgctttga actccttgca agcaatggtt ttatctattg acatcaacga agagttaact
     1321 gcatttggaa tattcgagta tggattgttt ccattgtcat cggaattgtc caagagtgaa
     1381 gttttgcaaa ccgatattca cggttttgtc aagacgcgga ctgaaacatt gaccttggtc
     1441 agcaaggttt atcttcacta ctgtgtcgac ttgaagcagg aagaaactga cggtgtttgg
     1501 ttaggaatat tggataactt tattacattc cacgaccaag aaagcaagct caagaaagag
     1561 aattcgttca aagaagcagg agccgaatta ctcaaaaaca tgattctagt actacaagac
     1621 aatggtactt tgagcaagga caaagagatc ctctggaacg aatcatggct gaagatagat
     1681 aaggtttatc ccggtttaag agacgaattg gatccggagc cgttgtctga gaagaataaa
     1741 gagaatatag tcgacacggc tgctgagggt ggagaagaga agaaaatcga aacagacgga
     1801 gataactaat ctgcaataaa tattacactt atgtaatcta atggtttctt aagaaagtag
     1861 tcgttatgga gaagtagtat tttgtatttt agttactttc ataaatatag caagcttatg
     1921 ttgcatctca aacagtgcga aaaatatttt agtctatttt atagtatcta caaacaaatt
     1981 ttcaattcat cacagacttt ctttgtgtac tagcaacacc ctaaaaacat tcgacatcta
     2041 gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     2101 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2161 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
     2221 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
     2281 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
     2341 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     2401 gggttgttac tcgctcacac ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     2461 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcag
     2521 ttaaggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
     2581 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca
     2641 ggatatgtgg cggataaagt tctacttagt ccccggggat ccgtacactt attgttaact
     2701 atgaaacata tacttatgga caaggttcac tgatttggac tgaaaagcaa aaacgagaaa
     2761 tgagtcaacg tctgagatca atccctagac cacccgggaa ggtcatgtgt ttcatgaagt
     2821 acgataaggt tggtaaccga ttgactcatt ggttcgtggc ggagaagtac gcagagtaaa
     2881 accggggccg attcgtggta aattctggaa tgatccagag gcgcgacatt tatgcagaca
     2941 atttgtgttt tgtcgcaaac gatgttatag cgaaattttt cactctgtca gataaatgga
     3001 ttttgtcaaa agggggaagt agaaggagaa tgggcccgag atgttctgcc aaattctcag
     3061 tagcataatg tgaaagaagc ccttacattg tccagcctct ggcatcatta aaaaccgtag
     3121 cggaaaccaa ttgtctctgt tcttccctgg cacaccctgg tagccccacc cagttgtagt
     3181 acatctcaca cgctggcaac ttgggacaat cagcaacttt tttttctttt aattttttca
     3241 gcgcgacatt ttgcctcttc tgcgagaaca gactttttca cctccatctc accccccttt
     3301 gcacttatat aaattggacc agttcctccc attgtagaaa aaattttgct ggaccttttt
     3361 ctcttttttt tgtcctttag tttcatacaa tctaagtcta tctacatgta caatgggtaa
     3421 ggaaaagact cacgtttcga ggccgagatt aaattccaac atggatgctg atttatatgg
     3481 gtataaatgg gctagagata atgtcgggca atcaggtgcg acaatctata gattgtatgg
     3541 gaagcccgat gcgccagagt tgtttttgaa acatggcaaa ggtagcgttg ccaatgatgt
     3601 tacagatgag atggtcagac taaactggtt gacggaattt atgcctcttc cgaccatcaa
     3661 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccggcaaaac
     3721 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgttggc
     3781 agtgttcttg agaagattgc attcgattcc tgtttgtaat tgtcctttta acagcgatag
     3841 agtatttcgt ctcgctcagg cgcaatcaag aatgaataac ggtttggttg atgcgagtga
     3901 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataagtt
     3961 gttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     4021 ttttgacgag gggaaattaa taggttgtat tgatgttgga agagtcggaa tcgcagacag
     4081 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     4141 aagacttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     4201 gatgctcgat gagtttttct aagcggccgc taaccacttg caaaatcctt tgtattcttg
     4261 ttctgcaaac attttgtcca atctcttatc tttctacgat gttgaagaac atacattttt
     4321 gttagtccag cttgttatcc tttttttatt aaataaaaaa ttttattgtt aaagttgttt
     4381 ttcttctttg tctgtcctgt tttaactcat atatattcta tatattacag aaagaaagta
     4441 gatcatctaa aataaacatt tatttgatga gacaaagtag gttttttaga atacaatgta
     4501 gcatcctcta atatacaata gagttgtttt ataaaagcga tacatttaat attatgacag
     4561 gactggtgaa gtgtatagaa ttgtgctaat ctggcaaacg aacccaagaa gacgttaaag
     4621 caaatagtgc attttgtatt acaatcaagt tcgtcgagat cctcgttagt atttccgtga
     4681 aggcagccat tgtgaaaatt gaacatcacc tgaggtattt tagccaccag aagcggcggt
     4741 acggaagaaa gtgtgtacga tggttggtgg tggaattgcg tgcatgcctg atggggcaat
     4801 attaattaga tagagctttg gtgatattag tggataatag aattcacaga gaagacatca
     4861 ggagcaattt ccaagagcca ttgatgatgt aattgcccca acagcaagat tcagatctga
     4921 caattgacca ccgttttgta gaagcaaaaa atcgtagatt atcaccaaga gggtttttca
     4981 ccgaaccagc aaatagaaac tatgccgtag aactcgccca ggcttttttg ctagcacttt
     5041 ccagcagtag aaccgtccaa ttaagtcaac aggaaccatt gaggtcgagc ccaaccacct
     5101 gaaccccctc acggtcgtgt ccctattatt gatccagagg gtgccagttt cggtagccaa
     5161 tattggttca tgggtttcta tggcccggag tgagtttgca ggttggcccc ggcgccgtct
     5221 gcaggatggg agttatagcg gccaaacttc acatttcgaa atcctgctgc agccaatctg
     5281 aagaattaat ataaattcgt gtcgaatcgc cgtctgtgaa atttcagtac ttgattttct
     5341 tttcttcttc tttttctctt ttgtttcttc agaatcaata cacatttttt cttccctata
     5401 aacaattcat cggtaccatg gtgaagcgtg agaaaaatgt catctatggc cctgagcctc
     5461 tccatccttt ggaggatttg actgccggcg aaatgttgtt tcgtgctctc cgcaagcact
     5521 ctcatttgcc tcaagccttg gtcgatgtgg tcggcgatga atctttgagc tacaaggagt
     5581 tttttgaggc aaccgtcttg ttggctcagt ccctccacaa ttgtggctac aagatgaacg
     5641 acgtcgttag tatctgtgct gaaaacaata cccgtttctt cattccagtc atcgccgcat
     5701 ggtatatcgg tatgatcgtg gctccagtca acgagagcta cattcccgac gaattgtgta
     5761 aagtcatggg tatctctaag ccacagattg tcttcaccac taagaatatt ttgaacaaag
     5821 tcttggaagt ccaaagccgc accaacttta ttaagcgtat catcatcttg gacactgtgg
     5881 agaatattca cggttgcgaa tctttgccta atttcatctc tcgctattca gacggcaaca
     5941 tcgcaaactt taaaccactc cacttcgacc ctgtggaaca agttgcagcc attttgtgta
     6001 gcagcggtac tactggactc ccaaagggag tcatgcagac ccatcaaaac atttgcgtgc
     6061 gtttgatcca tgctctcgat ccacgtgtgg gcactcagtt gattcctggt gtcaccgtct
     6121 tggtctactt gcctttcttc catgctttcg gctttagcat tactttgggt tactttatgg
     6181 tcggtctccg cgtgattatg ttccgccgtt ttgatcagga ggctttcttg aaagccatcc
     6241 aagattatga agtccgcagt gtcatcaacg tgcctagcgt gatcttgttt ttgtctaaga
     6301 gcccactcgt ggacaagtac gacttgtctt cattgcgtga attgtgttgc ggtgccgctc
     6361 cattggctaa ggaggtcgct gaagtggccg ccaaacgctt gaatcttcca ggtattcgtt
     6421 gtggcttcgg cctcaccgaa tctaccagcg ctaacattca ctctctcggt gatgagttta
     6481 agagcggctc tttgggccgt gtcactccac tcatggctgc taagatcgct gatcgcgaaa
     6541 ctggtaaggc tttgggtccg aaccaagtgg gcgagttgtg tatcaaaggc cctatggtga
     6601 gcaagggtta tgtcaataac gttgaagcta ccaaggaggc catcgacgac gacggctggt
     6661 tgcattctgg tgattttgga tattacgacg aagatgagca tttttacgtc gtggatcgtt
     6721 acaaggagtt gatcaaatac aagggtagcc aggttgctcc agctgagttg gaggagattt
     6781 tgttgaaaaa tccatgcatt cgcgatgtcg ctgtggtcgg cattcctgat ttggaggccg
     6841 gcgaattgcc ttctgctttc gttgtcaagc agcctggtaa agaaattacc gccaaagaag
     6901 tgtatgatta cttggctgaa cgtgtgagcc atactaagta cttgcgtggc ggcgtgcgtt
     6961 ttgttgactc catccctcgt aacgtaacag gcaaaattac ccgcaaggag ttgttgaaac
     7021 aattgttgga gaaggccggc ggttagctta attaagcatt ttagacaagt gcctatttat
     7081 tcagattgat tttttatatt gaatatggat gttgattacg acttgtgtta cgaagcatga
     7141 acatgtgatt tgaaaaagtg tttacgaata aaagaacttt ttttgcaacc gttgctagtt
     7201 gaagtgtaga ctctgaatgc gaagggttgc aaatgtgccc agtagaaaaa atattccata
     7261 tttttctgaa cttaattcaa tgaaatacga gtattactac aactgccaat atcggaacaa
     7321 tttaacgaga gcaagctata taaaatgaat gttcatttac acaagctaga accaaaactt
     7381 gaattaaaat ttaacataac taaatgtaac tagataaaag agagacataa ccaaacgttc
     7441 agaaggcaga actagtaaag ttctacttag tccccgggga ccatgattac ggattcactg
     7501 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     7561 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     7621 tcccaacagt tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa
     7681 gcggtgccgg aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc
     7741 tcaaactggc agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt
     7801 acggtcaatc cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt
     7861 aatgttgatg aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac
     7921 tcggcgtttc atctgtggtg caacgggcgc tgggtcagtt aaggccagga cagtcgtttg
     7981 ccgtctgaat ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg
     8041 gtgctgcgct ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatcttaagg
     8101 tggccaactc atatgaagaa gacaagccaa gtgtaaaata tgtaaaattt tgtaaaaatc
     8161 gtaaaactgt aatgtacatc gttaagtatt tacaaacaac tatatgatta tttacaagca
     8221 gaggcctatt ccaaaatttc gaattccagt aagtcgtcag tcaatctggt tctcattatt
     8281 tcaaaaacca agagggccac ggatgtagcc ggtattgttg tcaatgcatt gcggacaaaa
     8341 cctttgtatg ccatttgata ccaagtcaac tttgattttg tcttcatcct aaggatttgg
     8401 ttatatgtat caatgtatga gttgtagtac aaggttatgg agggtttgta tgaacgcgta
     8461 gctgatgcat tgtagaaatc aagagcttca agtctagaaa gatgaatctt ctgaatcttg
     8521 gtgattgggt attgtattgc caacaacgag aaagcggcag atgcaccagc tacgagaata
     8581 aaggttgacc tcaatatctt tttggttctc gtacgctcca agctcaatag tcgttcgtct
     8641 atctgttctt cgttcatatt tgagtattgt cgtaacttgc ttttgatgga ttctttgctt
     8701 cgtctataaa cattgacaac cttgaaagtt gctgtatagc cttgagtctt gacgaactca
     8761 aaggtagaga agtagaaggc aaatccaagc gattctttta caagcgaaaa actataccca
     8821 gcaaaaactc caaccaatcc gacttcttta agtttgttca aaccgtacat ccagaggttt
     8881 tggtgtgaac cactcaacat ttctgctgct gtcagtctag tatatatggc gtctacaggt
     8941 gctgctgcga gtgactgaac ggcaccagca ataaaaccag ctctccatgt atcgaatggg
     9001 gagtaataga acaattctcg gggtttatct gcttttgggg tatgcagact gttgaatcga
     9061 tccaaagccg tcaagtaagt tccgtacaat atcaaccctg tggccgaatt ggctacaaga
     9121 ggaggcaaca cctgatcagg gataaatctc catccctctt tctttactac attgacaagc
     9181 attcctatcg aggaatgcgt tctgaattta tacggtttct cgtggatgtt atcgtgtttg
     9241 ttagccaact cacgtacata ggctagatag tcaaatcgca gaggacgaaa taacttctct
     9301 ggtgttctta ggtagagaga ctgaagctgg tacataagag ctctcatacc agcggctgaa
     9361 agtgaaacag tttggttgct gctcatctta ggtggaatgt tggaagcagt gtagtccgta
     9421 atttcactac tgtccaggga ggcaatcgca gaatcattaa gtatggggct aggtttcata
     9481 ctattgagaa ccattgagaa aataggcatt agagacctta ttgtaacttc cataggttcc
     9541 acggaaactc ctttctcatt tagtgtatgt cgtttttcca ttgaagtaaa ttttatgtgt
     9601 atctatacgt tcaaatggat gagtgattgt gtttggcaat atttctatag ttttagcttt
     9661 gggttggggt atcatttatc tataagaggt aagtctacta taaccagttt atgaaagttg
     9721 tagatccaga gtttctatat gaaagtaaac atactggacc aacttgcgca gttgatttag
     9781 cttagtctcg atttcatttt ccatattggt acttagagaa tttgtatgcg aaaattataa
     9841 tcgggaaaaa ccagaagact ccgtttcttg tcaaaactaa tgttcctcca acatgtatgc
     9901 accatatcca aggtggattg tcttcgaaat ttttattcat tttttgtaca atattctttg
     9961 tattctaatt aattcttttt gctactataa tcttagaatc cttgtatcgt tgtgctattg
    10021 ctcgtctact ccgtatttat ctactggtct ctgtacaaga tgatctcact ctgcttgaag
    10081 tatcttctcg catcttcatc gatcaaaaca agcgacaagt acgtccttgc ggaaaacttc
    10141 ttgtttatgt cgatatcccg cggcttaatt aaagctagcc agcgcttgag ctccatcgga
    10201 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    10261 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    10321 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    10381 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    10441 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    10501 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    10561 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    10621 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    10681 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    10741 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    10801 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10861 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10921 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10981 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    11041 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    11101 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    11161 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    11221 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    11281 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    11341 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    11401 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    11461 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    11521 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    11581 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    11641 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    11701 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    11761 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    11821 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11881 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11941 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    12001 cgaaaagtgc cacctgacgt c
//
