LOCUS       sequence-409775-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for AWA neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  TITLE     Froekjaer-Jensen lab worm expression plasmids
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSAH80 - [AWAp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1911..1927)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1911..1927)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1924..1946)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2099..2687)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2179..2198)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2804..2828)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2928..3719,3720..3788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3551..3570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3789..3893)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3992..4014
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4006..4023
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4007..4023
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(join(4647..4663,1..17))
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acattatacg aagttatgat atcaaaaatg attacgccaa gctgtaagtt taaacatgat
       61 cttactaact aactattctc atttaaattt tcagagctta aaaatggctg aaatcactca
      121 caacgatgga tacgctaatg ccaaatgtaa catctttcat tgttcataaa aatatacgtg
      181 ttcgatagtt gagtttcatg agtcaacaat tagaactaag tcagaatgtt ttgaaatttt
      241 tgaaataaaa agctacgatt caataactcc tatcttgatt ttatgatttt gacagaaccg
      301 tcatcattct cattactcat tttattcttt tcgtgtggaa atttcatgaa aaacaactac
      361 agaaaaaact acaaattttg ttcttaaagt aacacaataa aagggaattc ttagtgggat
      421 tttagtcaca cttccgagaa aaagagacca aaaatggggc cctctaaggg cgaagaattg
      481 ttcacgggag tagtcccaat cttagtcgag cttgacggag gtgagtttta ttatcgattt
      541 tcgggatttt ctacttgtaa ttaccttttg tttttaataa gttgcttttt tccagatgtt
      601 aatggccaca agttttccgt gtctggagaa ggagaaggcg acgctaccta cggtaagctg
      661 accctgaagt tcatttgcac tactggcaag ttaccagtac catggccgac cctggtgaca
      721 acattttgct acggggttca atgcttctcc agatacccag gtaggttttc agtttcaatt
      781 tttgttgcaa ctacctcaac tctatgtttt cagatcatat gaaaagacac gactttttta
      841 aatccgccat gccagagggt tacgttcaag aacgtaccat tttttttaag gacgatggta
      901 actacaagac acgtgcggag gttaaatttg aaggcgatac tctcgtgaac cgtattgagc
      961 tcaaggtaaa tttttcaagc ttctaaattc caaaataaac cattttaatt tcttaattcc
     1021 agggaatcga tttcaaggaa gatggaaaca ttttgggcca caagcttgaa tataattata
     1081 atagtcataa tgtctacatt atggccgaca aacaaaaaaa cggtattaag gttaacttca
     1141 agattcgcca caatatcgaa gatggatctg tccaacttgc tgaccactac caacaaaata
     1201 ctccaattgg agatggaccg gttttgcttc cagataacca ctacctttcc actcagtctg
     1261 ccctctctaa ggatccgaat gaaaaacgtg accatatggt tcttttggag ttcgttaccg
     1321 cagcaggaat tactcacgga atggatgaac tgtacaagcc cgggtgatgg tctctgagct
     1381 atgcaaaatc ctttcaagca ttcccttctt ctctatcact cttctttctt tttgtcaaaa
     1441 aattctctcg ctaatttatt tgctttttta atgttattat tttatgactt tttatagtca
     1501 ctgaaaagtt tgcatctgag tgaagtgaat gctatcaaaa tgtgattctg atatccagga
     1561 aacggagaat ctgtgcggca atgtcttatc atttgaaacg cgccgtatcg atgctgacat
     1621 catgcggctc ccgtgttagt cgagaagatg ggtacgggcc tttattcatc caacactcta
     1681 cggcttcttc aagagctagt agggcaccct gtagttggaa agggattatt tcgtatggcg
     1741 agcccatacc gtctttcttg cggaagctta acacgatagg aagttggata gtttcgaacg
     1801 atggttccat acggaacgct gtctcaagga tgagtctgac ggagtgtaac tcgaaggcta
     1861 ggtggaggct cagtgatgat aagtctgcga tggtggatgc atgtgtcatg gtcatagctg
     1921 tttcctgtgt gaaattgtta tccgctcaga gggcacaatc ctattccgcg ctatccgaca
     1981 atctccaaga cattaggtgg agttcagttc ggcgtatggc atatgtcgct ggaaagaaca
     2041 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2101 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2161 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2221 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2281 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2341 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2401 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2461 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2521 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2581 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2641 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2701 tcttttctac ggggtctgac gctctattca acaaagccgc cgtcccgtca agtcagcgta
     2761 aatgggtagg gggcttcaaa tcgtcctcgt gataccaatt cggagcctgc ttttttgtac
     2821 aaacttgttg ataatggcaa ttcaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2881 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2941 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3001 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3061 ataccgcgag agccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3121 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     3181 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     3241 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     3301 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     3361 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     3421 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     3481 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     3541 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3601 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3661 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3721 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3781 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3841 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3901 ccccgaaaag tgccagatac ctgaaacaaa acccatcgta cggccaagga agtctccaat
     3961 aactgtgatc caccacaagc gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     4021 agtcatgcat aatccgcacg catctggaat aaggaagtgc cattccgcct gacctaagtg
     4081 cttttcgaga tcgccaaacc ggagaacgat caggagaatg atgagtcgcc acaggagtcg
     4141 gcaagatacg tgcgttatag atttgctcca aactttttga aactcaaaac ggtcggagca
     4201 actgtggaat tcaaagtagg agacatccca atccatcatt tccgaatgat cgaacgtcac
     4261 ttcttcaaag atcgccttct gaagtgtttt gactttgaat tcggattctg tattccgaat
     4321 tcacgaaaca actgtgaaca tatctatgag ttccctcaac tctctcaaca actcagtgag
     4381 tcattattct aaaaagtaca attcaaaaga ctaatctctt tttcagtgga cgacatgatc
     4441 aacaatccaa acgagactcg ttctgacagc ttctatttcg tcgataacaa actcgtcatg
     4501 cacaacaaag ccgactactc atatgatgca taaatcttca ttgttgagtt tatcttgttg
     4561 atttttgaat aaattatcaa ctctttactt tttaatgggt tatgaaataa ataaacattg
     4621 aaaactgata aacaacgttc atctctataa cttcgtatag cat
//
