LOCUS       sequence-409787-        4663 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gfp BioPart for PVQ neurons. (1) Mark neurons or (2) cell-specific 
            expression of transgenes (N or C-terminal fusions, or untagged). 
            Compatible with single-copy insertion (MosTI) or arrays..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   AlHarbi S, Frokjaer-Jensen C
  TITLE     Characterizing a standardized BioPart for PVQ-specific expression in
            C. elegans.
  JOURNAL   MicroPubl Biol. 2023 Jun 22;2023:10.17912/micropub.biology.000870. 
            doi: 10.17912/micropub.biology.000870. eCollection 2023.
  PUBMED    37426742
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2023-06-22"; volume: 
            "2023:10.17912/micropub.biology.000870. doi"; pages: " 
            10.17912/micropub.biology.000870. eCollection 2023"
COMMENT     Sequence Label: pSAH50 - [PVQp | gfp | tbb-2 UTR]
FEATURES             Location/Qualifiers
     source          1..4663
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1888..1904)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1888..1904)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1901..1923)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2076..2664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2156..2175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2781..2805)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2905..3696,3697..3765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3528..3547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3766..3870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3969..3991
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3983..4000
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3984..4000
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4624..4657)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aaaaatgatt acgccaagct gtaagtttaa acatgatctt actaactaac tattctcatt
       61 taaattttca gagcttaaaa atggctgaaa tcactcacaa cgatggatac gctaatgcca
      121 ctttgatgtt tcaaaagttt tcctaatcta tatgtttttt cgcagcctat tatctcaaaa
      181 acttattatt tatttattta tctaggttat tacgggaatg gatgaggggg tgacgttttt
      241 gagtttttga gtcgcactta attagaaaag ctatataata actctagata taattactcg
      301 actcacttaa tagccttgat agctcgctat aattgaaata taaatagtga gatgagccat
      361 ttaaatcgac atttcgagac ttttttctcg cgaaaaagtc aggctttttc acagaaaaaa
      421 gagaccaaaa atggggccct ctaagggcga agaattgttc acgggagtag tcccaatctt
      481 agtcgagctt gacggaggtg agttttatta tcgattttcg ggattttcta cttgtaatta
      541 ccttttgttt ttaataagtt gcttttttcc agatgttaat ggccacaagt tttccgtgtc
      601 tggagaagga gaaggcgacg ctacctacgg taagctgacc ctgaagttca tttgcactac
      661 tggcaagtta ccagtaccat ggccgaccct ggtgacaaca ttttgctacg gggttcaatg
      721 cttctccaga tacccaggta ggttttcagt ttcaattttt gttgcaacta cctcaactct
      781 atgttttcag atcatatgaa aagacacgac ttttttaaat ccgccatgcc agagggttac
      841 gttcaagaac gtaccatttt ttttaaggac gatggtaact acaagacacg tgcggaggtt
      901 aaatttgaag gcgatactct cgtgaaccgt attgagctca aggtaaattt ttcaagcttc
      961 taaattccaa aataaaccat tttaatttct taattccagg gaatcgattt caaggaagat
     1021 ggaaacattt tgggccacaa gcttgaatat aattataata gtcataatgt ctacattatg
     1081 gccgacaaac aaaaaaacgg tattaaggtt aacttcaaga ttcgccacaa tatcgaagat
     1141 ggatctgtcc aacttgctga ccactaccaa caaaatactc caattggaga tggaccggtt
     1201 ttgcttccag ataaccacta cctttccact cagtctgccc tctctaagga tccgaatgaa
     1261 aaacgtgacc atatggttct tttggagttc gttaccgcag caggaattac tcacggaatg
     1321 gatgaactgt acaagcccgg gtgatggtct ctgagctatg caaaatcctt tcaagcattc
     1381 ccttcttctc tatcactctt ctttcttttt gtcaaaaaat tctctcgcta atttatttgc
     1441 ttttttaatg ttattatttt atgacttttt atagtcactg aaaagtttgc atctgagtga
     1501 agtgaatgct atcaaaatgt gattctgata tccaggaaac ggagaatctg tgcggcaatg
     1561 tcttatcatt tgaaacgcgc cgtatcgatg ctgacatcat gcggctcccg tgttagtcga
     1621 gaagatgggt acgggccttt attcatccaa cactctacgg cttcttcaag agctagtagg
     1681 gcaccctgta gttggaaagg gattatttcg tatggcgagc ccataccgtc tttcttgcgg
     1741 aagcttaaca cgataggaag ttggatagtt tcgaacgatg gttccatacg gaacgctgtc
     1801 tcaaggatga gtctgacgga gtgtaactcg aaggctaggt ggaggctcag tgatgataag
     1861 tctgcgatgg tggatgcatg tgtcatggtc atagctgttt cctgtgtgaa attgttatcc
     1921 gctcagaggg cacaatccta ttccgcgcta tccgacaatc tccaagacat taggtggagt
     1981 tcagttcggc gtatggcata tgtcgctgga aagaacatgt gagcaaaagg ccagcaaaag
     2041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     2101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     2161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     2221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     2281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     2341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     2401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2701 ctattcaaca aagccgccgt cccgtcaagt cagcgtaaat gggtaggggg cttcaaatcg
     2761 tcctcgtgat accaattcgg agcctgcttt tttgtacaaa cttgttgata atggcaattc
     2821 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     2881 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     2941 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3001 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagagc cacgctcacc
     3061 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3121 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3181 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3241 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     3301 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     3361 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     3421 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     3481 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     3541 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     3601 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     3661 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     3721 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     3781 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     3841 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cagatacctg
     3901 aaacaaaacc catcgtacgg ccaaggaagt ctccaataac tgtgatccac cacaagcgcc
     3961 agggttttcc cagtcacgac gttgtaaaac gacggccagt catgcataat ccgcacgcat
     4021 ctggaataag gaagtgccat tccgcctgac ctaagtgctt ttcgagatcg ccaaaccgga
     4081 gaacgatcag gagaatgatg agtcgccaca ggagtcggca agatacgtgc gttatagatt
     4141 tgctccaaac tttttgaaac tcaaaacggt cggagcaact gtggaattca aagtaggaga
     4201 catcccaatc catcatttcc gaatgatcga acgtcacttc ttcaaagatc gccttctgaa
     4261 gtgttttgac tttgaattcg gattctgtat tccgaattca cgaaacaact gtgaacatat
     4321 ctatgagttc cctcaactct ctcaacaact cagtgagtca ttattctaaa aagtacaatt
     4381 caaaagacta atctcttttt cagtggacga catgatcaac aatccaaacg agactcgttc
     4441 tgacagcttc tatttcgtcg ataacaaact cgtcatgcac aacaaagccg actactcata
     4501 tgatgcataa atcttcattg ttgagtttat cttgttgatt tttgaataaa ttatcaactc
     4561 tttacttttt aatgggttat gaaataaata aacattgaaa actgataaac aacgttcatc
     4621 tctataactt cgtatagcat acattatacg aagttatgat atc
//
