LOCUS       sequence-409790-        9023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9023)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3-SREBP-1 (A317-318)
FEATURES             Location/Qualifiers
     source          1..9023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(136..156)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        141..470
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      321..456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     383..402
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             537..1331
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(591..610)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1201..1220
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1505..1626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1542..1561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1596..1615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1675..1691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1675..1691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1688..1710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1699..1715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1723..1729,1730..1747,1748..1753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1768..1789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1906..1923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2077..2665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2157..2176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2836..3627,3628..3696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3459..3478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3697..3801)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3876..3895)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4067..4446
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4447..4650
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4601..4621
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4695..4714
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4695..4713
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4739..4748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8446..8463)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8452..8676
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(8722..9023,1..127)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8809..8828)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(9019..9023,1..17)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
       61 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      121 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      241 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      301 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      361 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
      421 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
      481 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
      541 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
      601 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
      661 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
      721 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
      781 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
      841 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
      901 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
      961 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     1021 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     1081 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     1141 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     1201 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     1261 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     1321 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     1381 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     1441 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     1501 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1561 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     1621 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     1681 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     1741 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     1801 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     1861 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     1921 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1981 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2041 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2101 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2161 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2221 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2281 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2341 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2401 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2461 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2521 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2581 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2641 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2701 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     2761 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     2821 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     2881 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     2941 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3001 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3061 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3121 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3181 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3241 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3301 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3361 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3421 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3481 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3541 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     3601 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     3661 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     3721 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     3781 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     3841 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     3901 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     3961 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     4021 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     4081 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4141 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4201 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     4261 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     4321 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4381 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4441 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     4501 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4561 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     4621 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     4681 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcggatccgc
     4741 caccatggcc tctatgaccg gcggccagca aatgggtatg gcctctatga ccggcggcca
     4801 gcaaatggga gcatcgatgg acgagccacc cttcagcgag gcggctttgg agcaggcgct
     4861 gggcgagccg tgcgatctgg acgcggcgct gctgaccgac atcgaaggtg aagtcggcgc
     4921 ggggaggggt agggccaacg gcctggacgc cccaagggcg ggcgcagatc gcggagctat
     4981 ggattgcact ttcgaagaca tgcttcagct tatcaacaac caagacagtg acttccctgg
     5041 cctatttgac ccaccctatg ctgggagtgg ggcagggggc acagaccctg ccagccccga
     5101 taccagctcc ccaggcagct tgtctccacc tcctgccaca ttgagctcct ctcttgaagc
     5161 cttcctgagc gggccgcagg cagcgccctc acccctgtcc cctccccagc ctgcacccac
     5221 tccattgaag atgtacccgt ccatgcccgc tttctcccct gggcctggta tcaaggaaga
     5281 gtcagtgcca ctgagcatcc tgcagacccc caccccacag cccctgccag gggccctcct
     5341 gccacagagc ttcccagccc cagccccacc gcagttcagc tccacccctg tgttaggcta
     5401 ccccagccct ccgggaggct tctctacagg aagccctccc gggaacaccc agcagccgct
     5461 gcctggcctg ccactggctt ccccgccagg ggtcccgccc gtctccttgc acacccaggt
     5521 ccagagtgtg gtcccccagc agctactgac agtcacagct gcccccacgg cagcccctgt
     5581 aacgaccact gtgacctcgc agatccagca ggtcccggtc ctgctgcagc cccacttcat
     5641 caaggcagac tcgctgcttc tgacagccat gaagacagac ggagccactg tgaaggcggc
     5701 aggtctcagt cccctggtct ctggcaccac tgtgcagaca gggcctttgc cgaccctggt
     5761 gagtgccgca accatcttgg caacagtccc actggtcgta gatgcggaga agctgcctat
     5821 caaccggctc gcagctggca gcaaggcccc ggcctctgcc cagagccgtg gagagaagcg
     5881 cacagcccac aacgccattg agaagcgcta ccgctcctcc atcaatgaca aaatcattga
     5941 gctcaaggat ctggtggtgg gcactgaggc aaagctgaat aaatctgctg tcttgcgcaa
     6001 ggccatcgac tacattcgct ttctgcaaca cagcaaccag aagcttaagc aggagaacct
     6061 aagtctgcgc actgctgtcc acaaaagcaa atctctgaag gatctggtgt cggcctgtgg
     6121 cagtggaggg aacacagacg tgctcatgga gggcgtgaag actgaggtgg aggacacact
     6181 gaccccaccc ccctcggatg ctggctcacc tttccagagc agccccttgt cccttggcag
     6241 caggggcagt ggcagcggtg gcagtggcag tgactcggag cctgacagcc cagtctttga
     6301 ggacagcaag gcaaagccag agcagcggcc gtctctgcac agccgcggca tgctggaccg
     6361 ctcccgcctg gccctgtgca cgctcgtctt cctctgcctg tcctgcaacc ccttggcctc
     6421 cttgctgggg gcccgggggc ttcccagccc ctcagatacc accagcgtct accatagccc
     6481 tgggcgcaac gtgctgggca ccgagtctag agatggccct ggctgggccc agtggctgct
     6541 gcccccagtg gtctggctgc tcaatgggct gttggtgctc gtctccttgg tgcttctctt
     6601 tgtctacggt gagccagtca cacggcccca ctcaggcccc gccgtgtact tctggaggca
     6661 tcgcaagcag gctgacctgg acctggcccg gggagacttt gcccaggctg cccagcagct
     6721 gtggctggcc ctgcgggcac tgggccggcc cctgcccacc tcccacctgg acctggcttg
     6781 tagcctcctc tggaacctca tccgtcacct gctgcagcgt ctctgggtgg gccgctggct
     6841 ggcaggccgg gcagggggcc tgcagcagga ctgtgctctg cgagtggatg caagcgccag
     6901 cgcccgagac gcagccctgg tctaccataa gctgcaccag ctgcacacca tggggaagca
     6961 cacaggcggg cacctcactg ccaccaacct ggcgctgagt gccctgaacc tggcagagtg
     7021 tgcaggggat gccgtgtctg tggcgacgct ggccgagatc tatgtggcgg ctgcattgag
     7081 agtgaagacc agtctcccac gggccttgca ttttctgaca cgcttcttcc tgagcagtgc
     7141 ccgccaggcc tgcctggcac agagtggctc agtgcctcct gccatgcagt ggctctgcca
     7201 ccccgtgggc caccgtttct tcgtggatgg ggactggtcc gtgctcagta ccccatggga
     7261 gagcctgtac agcttggccg ggaacccagt ggaccccctg gcccaggtga ctcagctatt
     7321 ccgggaacat ctcttagagc gagcactgaa ctgtgtgacc cagcccaacc ccagccctgg
     7381 gtcagctgat ggggacaagg aattttcgga tgccctcggg tacctgcagc tgctgaacag
     7441 ctgttctgat gctgcggggg ctcctgccta cagcttctcc atcagttcca gcatggccac
     7501 caccaccggc gtagacccgg tggccaagtg gtgggcctct ctgacagctg tggtgatcca
     7561 ctggctgcgg cgggatgagg aggcggctga gcggctgtgc ccgctggtgg agcacctgcc
     7621 ccgggtgctg caggagtctg agagacccct gcccagggca gctctgcact ccttcaaggc
     7681 tgcccgggcc ctgctgggct gtgccaaggc agagtctggt ccagccagcc tgaccatctg
     7741 tgagaaggcc agtgggtacc tgcaggacag cctggctacc acaccagcca gcagctccat
     7801 tgacaaggcc gtgcagctgt tcctgtgtga cctgcttctt gtggtgcgca ccagcctgtg
     7861 gcggcagcag cagcccccgg ccccggcccc agcagcccag ggcaccagca gcaggcccca
     7921 ggcttccgcc cttgagctgc gtggcttcca acgggacctg agcagcctga ggcggctggc
     7981 acagagcttc cggcccgcca tgcggagggt gttcctacat gaggccacgg cccggctgat
     8041 ggcgggggcc agccccacac ggacacacca gctcctcgac cgcagtctga ggcggcgggc
     8101 aggccccggt ggcaaaggag gcgcggtggc ggagctggag ccgcggccca cgcggcggga
     8161 gcacgcggag gccttgctgc tggcctcctg ctacctgccc cccggcttcc tgtcggcgcc
     8221 cgggcagcgc gtgggcatgc tggctgaggc ggcgcgcaca ctagagaaac ttggcgatcg
     8281 ccggctgctg cacgactgtc agcagatgct catgcgcctg ggcggtggga ccactgtcac
     8341 ttccagcgga gaattctcag gcgcccccgt gccctacccc gaccccctgg agccccggtg
     8401 agcggccgct cgagtctagt gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     8461 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     8521 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     8581 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     8641 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     8701 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     8761 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     8821 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     8881 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     8941 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9001 agtccacgtt ctttaatagt gga
//
