LOCUS       sequence-409791-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of HA-KIF2C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Hong C, Schubert M, Tijhuis AE, Requesens M, Roorda M, van den Brink
            A, Ruiz LA, Bakker PL, van der Sluis T, Pieters W, Chen M, Wardenaar
            R, van der Vegt B, Spierings DCJ, de Bruyn M, van Vugt MATM, Foijer 
            F
  TITLE     cGAS-STING drives the IL-6-dependent survival of chromosomally 
            instable cancers.
  JOURNAL   Nature. 2022 Jul;607(7918):366-373. doi: 10.1038/s41586-022-04847-2.
            Epub 2022 Jun 15.
  PUBMED    35705809
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04847-2"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7918"; pages: "366-373"
COMMENT     Sequence Label: pLVX-KIF2C
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2205..2519
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2284..2302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2320..2338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2492..2516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2535..2561
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2535..2561
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2541..2567
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        4759..5258
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4794..4817)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5191..5210
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5279..5878
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5279..5298)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5775..5795
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5895..6483
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5948..5968)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6366..6377)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6690..7323
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7451..7467)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7451..7467)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7464..7486)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7475..7491
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7499..7505,7506..7523,7524..7529))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7544..7565
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7682..7699)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7853..8441)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7933..7952)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8612..9403,9404..9472))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9235..9254
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9473..9577)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9625..9759
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9662..9681)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9716..9735
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9927,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
     2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2521 ggagaaggat catgtacccc tacgacgtgc ccgactacgc ctccctcgga tccgccatgg
     2581 actcgtcgct tcaggcccgc ctgtttcccg gtctcgctat caagatccaa cgcagtaatg
     2641 gtttaattca cagtgccaat gtaaggactg tgaacttgga gaaatcctgt gtttcagtgg
     2701 aatgggcaga aggaggtgcc acaaagggca aagagattga ttttgatgat gtggctgcaa
     2761 taaacccaga actcttacag cttcttccct tacatccgaa ggacaatctg cccttgcagg
     2821 aaaatgtaac aatccagaaa caaaaacgga gatccgtcaa ctccaaaatt cctgctccaa
     2881 aagaaagtct tcgaagccgc tccactcgca tgtccactgt ctcagagctt cgcatcacgg
     2941 ctcaggagaa tgacatggag gtggagctgc ctgcagctgc aaactcccgc aagcagtttt
     3001 cagttcctcc tgcccccact aggccttcct gccctgcagt ggctgaaata ccattgagga
     3061 tggtcagcga ggagatggaa gagcaagtcc attccatccg aggcagctct tctgcaaacc
     3121 ctgtgaactc agttcggagg aaatcatgtc ttgtgaagga agtggaaaaa atgaagaaca
     3181 agcgagaaga gaagaaggcc cagaactctg aaatgagaat gaagagagct caggagtatg
     3241 acagtagttt tccaaactgg gaatttgccc gaatgattaa agaatttcgg gctactttgg
     3301 aatgtcatcc acttactatg actgatccta tcgaagagca cagaatatgt gtctgtgtta
     3361 ggaaacgccc actgaataag caagaattgg ccaagaaaga aattgatgtg atttccattc
     3421 ctagcaagtg tctcctcttg gtacatgaac ccaagttgaa agtggactta acaaagtatc
     3481 tggagaacca agcattctgc tttgactttg catttgatga aacagcttcg aatgaagttg
     3541 tctacaggtt cacagcaagg ccactggtac agacaatctt tgaaggtgga aaagcaactt
     3601 gttttgcata tggccagaca ggaagtggca agacacatac tatgggcgga gacctctctg
     3661 ggaaagccca gaatgcatcc aaagggatct atgccatggc ctcccgggac gtcttcctcc
     3721 tgaagaatca accctgctac cggaagttgg gcctggaagt ctatgtgaca ttcttcgaga
     3781 tctacaatgg gaagctgttt gacctgctca acaagaaggc caagctgcgc gtgctggagg
     3841 acggcaagca acaggtgcaa gtggtggggc tgcaggagca tctggttaac tctgctgatg
     3901 atgtcatcaa gatgatcgac atgggcagcg cctgcagaac ctctgggcag acatttgcca
     3961 actccaattc ctcccgctcc cacgcgtgct tccaaattat tcttcgagct aaagggagaa
     4021 tgcatggcaa gttctctttg gtagatctgg cagggaatga gcgaggcgcg gacacttcca
     4081 gtgctgaccg gcagacccgc atggagggcg cagaaatcaa caagagtctc ttagccctga
     4141 aggagtgcat cagggccctg ggacagaaca aggctcacac cccgttccgt gagagcaagc
     4201 tgacacaggt gctgagggac tccttcattg gggagaactc taggacttgc atgattgcca
     4261 cgatctcacc aggcataagc tcctgtgaat atactttaaa caccctgaga tatgcagaca
     4321 gggtcaagga gctgagcccc cacagtgggc ccagtggaga gcagttgatt caaatggaaa
     4381 cagaagagat ggaagcctgc tctaacgggg cgctgattcc aggcaattta tccaaggaag
     4441 aggaggaact gtcttcccag atgtccagct ttaacgaagc catgactcag atcagggagc
     4501 tggaggagaa ggctatggaa gagctcaagg agatcataca gcaaggacca gactggcttg
     4561 agctctctga gatgaccgag cagccagact atgacctgga gacctttgtg aacaaagcgg
     4621 aatctgctct ggcccagcaa gccaagcatt tctcagccct gcgagatgtc atcaaggcct
     4681 tgcgcctggc catgcagctg gaagagcagg ctagcagaca aataagcagc aagaaacggc
     4741 cccagtgaga attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     4801 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     4861 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     4921 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     4981 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     5041 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     5101 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     5161 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     5221 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     5281 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     5341 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     5401 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     5461 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     5521 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     5581 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     5641 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     5701 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     5761 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     5821 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg
     5881 ggcgcgtctg gaacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     5941 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     6001 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     6061 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     6121 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     6181 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     6241 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     6301 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     6361 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     6421 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     6481 cgcctggaat taattctgca gtcgagacct agaaaaacat ggagcaatca caagtagcaa
     6541 tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg aggaggtggg
     6601 ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag ctgtagatct
     6661 tagccacttt ttaaaagaaa agaggggact ggaagggcta attcactccc aacgaagaca
     6721 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     6781 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     6841 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     6901 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     6961 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     7021 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     7081 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     7141 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7201 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7261 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7321 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7381 cagagagtga gaggccttga cattgctagc gtttaccgtc gacctctagc tagagcttgg
     7441 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7501 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7561 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7621 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7681 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     7741 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     7801 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7861 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     7921 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     7981 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8041 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8101 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8161 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8221 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8281 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8341 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8401 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8461 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8521 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8581 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8641 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8701 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8761 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8821 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     8881 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     8941 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9001 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9061 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9121 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9181 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9241 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9301 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9361 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9421 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9481 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9541 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9601 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa
     9661 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9721 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     9781 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     9841 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     9901 taaatatgta ctacaaactt agtagtt
//
