LOCUS       sequence-409796-        9912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  inducible expression of HA-dnMCAK.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Hong C, Schubert M, Tijhuis AE, Requesens M, Roorda M, van den Brink
            A, Ruiz LA, Bakker PL, van der Sluis T, Pieters W, Chen M, Wardenaar
            R, van der Vegt B, Spierings DCJ, de Bruyn M, van Vugt MATM, Foijer 
            F
  TITLE     cGAS-STING drives the IL-6-dependent survival of chromosomally 
            instable cancers.
  JOURNAL   Nature. 2022 Jul;607(7918):366-373. doi: 10.1038/s41586-022-04847-2.
            Epub 2022 Jun 15.
  PUBMED    35705809
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-022-04847-2"; journalName: "Nature"; date: 
            "2022-07"; volume: "607"; issue: "7918"; pages: "366-373"
COMMENT     Sequence Label: pLVX-dnMCAK
FEATURES             Location/Qualifiers
     source          1..9912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2027..2142
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2205..2519
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2213..2231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2249..2267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2284..2302
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2320..2338
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2356..2374
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2391..2409
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2427..2445
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2492..2516
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2535..2561
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             2535..2561
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2541..2567
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     promoter        4744..5243
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4779..4802)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5176..5195
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5264..5863
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5264..5283)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5760..5780
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5880..6468
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5933..5953)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6351..6362)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6675..7308
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7436..7452)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7436..7452)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7449..7471)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7460..7476
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7484..7490,7491..7508,7509..7514))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7529..7550
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7667..7684)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7838..8426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7918..7937)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8597..9388,9389..9457))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9220..9239
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9458..9562)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9610..9744
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9647..9666)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9701..9720
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             join(9912,1..633)
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgagttt actccctatc
     2221 agtgatagag aacgtatgtc gagtttactc cctatcagtg atagagaacg atgtcgagtt
     2281 tactccctat cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac
     2341 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta tccctatcag
     2401 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgaggta
     2461 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2521 ggagaaggat catgtacccc tacgacgtgc ccgactacgc ctccctcgga tccgagtcgc
     2581 ttcccgctcg attgtttcct ggtctctcca tcaagattca acgcagtaat ggcttaattc
     2641 acagtgccaa cataagcaca gtgaacgtgg agaaatcctg tgtttcagtg gaatggatag
     2701 aaggaggtaa cacgaaaggc aaagagattg attttgatga tgtggctgca ataaacccag
     2761 aacttctaca gcttcttccc ttacacccaa aggacaatct gccactgcag gaaaatgtaa
     2821 cagttccgaa gcaaaaacgc agagcagtca actccaaaat tccagctcca aaagaaggtc
     2881 ttcgagcacg tgcaactcgc atggcaactg tcccagaggt tcgaattgcc actcaggaga
     2941 atgaaatgga ggtggagctg cctgtggcta caaactctcg aaagcagttt tcggttgcca
     3001 ctggcctccc taggccctcc tgtcctgcaa tgacagaatt accgttgtcg atggtcagcg
     3061 aggaggcaga agagcaagtc catcccaccc gaagcacatc ttctgcaaac cctgctcgga
     3121 ggaaagcatg tattgtgaag gaaatggaaa aaatgaagaa caagcgggaa gagaagaggg
     3181 cccagaactc ggaaataaga ataaagcgag ctcaggaata tgacagcagc tttccaaact
     3241 gggaatttgc ccggatgatt aaagaattcc gggttactat agaatgtcac ccacttactc
     3301 tgactgatcc tacggaagag cacaggatct gcgtgtgtgt gaggaagcgc ccactgaata
     3361 agcaagaact ggccaagaaa gaaatcgatg tgatttctgt tcccagcaag tgtttgctct
     3421 ttgtgcatga acccaaattg aaagtggact tgacaaagta tctggagaat caggcattct
     3481 gcttcgattt tgcctttgat gaaacagctt caaatgaagt cgtctacagg ttcacagcaa
     3541 ggccactggt acagacgatt tttgaagggg gaaaagcaac ctgctttgcc tatgggcaga
     3601 cgggcagtgg gaagactcac acaatgggtg gagacctgtc tggtaaatct cagaatacat
     3661 ctaaagggat ctatgcaatg gcttcccggg atgtcttcct tctgaagagt cagcctcgct
     3721 accggaactt aaacttggaa gtttatgtga cattctttga gatctacaat gggaaggtgt
     3781 ttgatctgct caacaagaag gccaagctac gtgtactaga agacagcaag cagcaggtgc
     3841 aggttgtggg acttcaggag tacctggtta actgtgctga tgatgtcatc aagatgctca
     3901 acatgggcag tgcctgcagg acttctggac agacttttgc caactccaat tcttcccgct
     3961 cccatgcctg cttccagatt cttcttcgag ccaaagggag attgcatggc aaattctcct
     4021 tggtggatct ggcagggaat gaacgagggg ctgacacttc tagtgctgac cgccagactc
     4081 gcatggaggg tgcagagatt aacaagtctc tcctagcact gaaggagtgc atcagggcgc
     4141 tgggacagaa caaggctgcc accccattcg ctgagagcgc actgactcag gtgctaaggg
     4201 attcattcat tggggagaac tcgaggactt gcatgattgc catgatctca ccaggcataa
     4261 gctcctgtga atatacttta aacacgctga gatatgcaga cagagtcaag gagctgagcc
     4321 cccacagcgg gctcagtgga gagcagccaa ttcaaatgga aacggaagag atggaagcca
     4381 gctctaatgg gacctcgctg gcagtcaatt tcaaggaaga agaggagctg tcttcccaga
     4441 tgtccagctt taatgaagcc atgagtcaga tcagggagct ggaagagagg gccatggagg
     4501 agctcaggga gatcatacag caaggaccag gctggcttga gctctctgag atgactgatc
     4561 agccagacta tgacttagag acctttgtga acaaagcaga atctgccctg actcagcaga
     4621 ctaagcactt ctcagccctg cgagaagtca tcaaggccct gcgtgtggcc atgcaactgg
     4681 aagaacaggc tagcaaacag atgaacagca agaaacggca ccaatgaacg cgtgaattct
     4741 accgggtagg ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc
     4801 tgggcacttg gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta
     4861 ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag
     4921 tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag
     4981 cacgtctcac tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc
     5041 tttggggcag cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa
     5101 ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt
     5161 cctccggagg cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct
     5221 cttcctcatc tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc
     5281 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
     5341 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
     5401 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
     5461 cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
     5521 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
     5581 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
     5641 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
     5701 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
     5761 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
     5821 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc tgacgggcgc gtctggaaca
     5881 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     5941 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     6001 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     6061 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     6121 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     6181 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     6241 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     6301 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     6361 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     6421 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct ggaattaatt
     6481 ctgcagtcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     6541 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     6601 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6661 agaaaagagg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     6721 gtggatctac cacacacaag gctacttccc tgattagcag aactacacac cagggccagg
     6781 ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa
     6841 ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg
     6901 gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca
     6961 tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta
     7021 caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg
     7081 gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt
     7141 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     7201 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     7261 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     7321 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
     7381 cttgacattg ctagcgttta ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7441 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     7501 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     7561 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     7621 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     7681 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     7741 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     7801 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     7861 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     7921 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     7981 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8041 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8101 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8161 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8221 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8281 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8341 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8401 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     8461 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     8521 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     8581 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     8641 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     8701 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     8761 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     8821 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     8881 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     8941 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9001 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9061 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9121 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9181 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9241 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9301 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9361 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9421 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     9481 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     9541 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     9601 cgggagatca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     9661 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     9721 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     9781 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     9841 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     9901 aacttagtag tt
//
