LOCUS       sequence-409798-        7183 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Rogers KW, ElGamacy M, Jordan BM, Muller P
  TITLE     Optogenetic investigation of BMP target gene expression diversity.
  JOURNAL   Elife. 2020 Nov 11;9:e58641. doi: 10.7554/eLife.58641.
  PUBMED    33174840
REFERENCE   2  (bases 1 to 7183)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2020-11-11"; volume: "9:e58641. doi"; pages: " 
            10.7554/eLife.58641"
COMMENT     Sequence Label: pCS2-Opto-BMPR2a
FEATURES             Location/Qualifiers
     source          1..7183
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      619..1074
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(706..725)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     916..937
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1100..1204
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1205..1273,1274..2065)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1423..1442)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2236..2824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2725..2744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2978..2995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3112..3133
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3148..3153,3154..3171,3172..3178)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3186..3202
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3191..3213
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3210..3226
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3210..3226
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3245..3265
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3247..3265
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    3378..3512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3415..3434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3469..3488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(3566..3592)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(6608..6649)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      6646..6655
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6694..6712)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6695..6712)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(join(6747..7183,1..548))
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgtcaatagg aaagaccata tatagagacc attgacgtca atgggggagt ggctatgggc
       61 ggtattagga agccccatat atggtatatg ggaccgccca ttgggagggg ctatctacgt
      121 caataggaaa acccatatat ggaatactat atggcatagg gccaatacat agtattgaac
      181 ctggccaata gccatattgg catagggcca tattggatat tgcctatata ttgatcctgg
      241 catatagcca atatggccgc cattattggc accatgccaa ttcaatatgg cggacttggc
      301 acagggccaa gacccctcct caaacttggc accgtgccaa gtagacccct ccccagttgg
      361 cacagtgcca ggtccgccat attgaattgg catggtgcca agtccgccat attgaattgg
      421 ctggggtcca gatccaccat attgaattgg cacgagtcca tatacgccat attgaattgg
      481 cacaggtcca gatccaccat attgaattgg catgagtcca tatacgccat attgaattgg
      541 cggtcgactg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      601 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
      661 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
      721 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
      781 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
      841 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
      901 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
      961 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1021 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     1081 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     1141 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     1201 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     1261 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     1321 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     1381 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     1441 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     1501 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     1561 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     1621 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     1681 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     1741 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     1801 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     1861 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     1921 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     1981 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     2041 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     2101 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     2161 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     2221 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     2281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2821 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2881 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2941 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     3001 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     3061 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     3121 ttagctcact cattaggcac cccaggcttt acactttatg ctcccggctc gtatgttgtg
     3181 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     3241 gcgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggccca atgcattggc
     3301 gccgcggccg cgaattaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     3361 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     3421 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     3481 aactcatcaa tgtatcttat catgtctgga tctacgtaat acgactcact atagttctag
     3541 aggctcgaga ggccttgaat tcttatgcgt agtctggtac gtcgtacgga taactagacc
     3601 cgggctttct gcgcagcatg ttactggtgc gcacaccttt gtactcaatg ttctgctcgt
     3661 tgaccagcag cttggcataa tcttcgctca cctttgactg cactccgacg tagttgacaa
     3721 tattgccctt agaatctctg agtccagcca cgaagaagag attccagaag gttgtgccat
     3781 cctgtctata attcagcaga cagacactgg tatcaacgcc tttggtgatg gcattcctga
     3841 tcttatccac agctcttggg tctgtttctg gcccttgcag aaacctgcag ttcctgccca
     3901 ggatctggtc gagagaatag cctgtcagtg tcagaaaccc tctactggcg tagacgatag
     3961 ggttgtctgg gagggaggcg tctgtaatga caaaattctg ttgtgccatt tgcagagcct
     4021 tcacgagact gtagtcaggg ctacctccgc caccacttcc accgcctcca gaacctcctc
     4081 caccaaagtc acagtcatta ggatctgaga agcgagatcc tcctcgactg cctagataga
     4141 cagccgaagc tgatttaggt ctgctggtcc cagcctgatt ctgattctgc ccgaggcctc
     4201 ctccgtgacg actgttgttg ttgacttcgg catccagtgt gtcagtggtg ataacccagc
     4261 tggcaggacg ccacttctta agtgcgtaag gcgtctttac ccgcttcttg attttatccc
     4321 ctgacccctc tgtcccatcg ccgacagacc tgcctcctag taatggcagt gttgtgatgg
     4381 acagatccag agagttgggt ctctctggtc tgcgcactcg gttctgtctg agcagagcct
     4441 ctccgtgtgt ctgtgtctgg ctttgggcct gtggaggaat ggcggttgaa gcagaacacg
     4501 ggccaggcag cacaaccggc tccaccgtag aagacgacga agcatcccct gtcggaccga
     4561 cactctcatt gttctctcct ccctctcctc caccctctgt ctctccatca ccatctccct
     4621 caccattgtt gttattgctg ttgttgttgt ttgcattgtc aggatgcgcc tctcttctca
     4681 gcagaggctc ctgctcatcg ggactggtgt ttaagaggcc gaaggtcaag tcctcgttgg
     4741 ctgaacttcc tgctgactcg gctgctggtt ctgttgcata tttattcatt gctgccgccg
     4801 catcgttgtt ggcaactgta atcagccggg cttcatgcgt cgcattcacc acactgcttt
     4861 tagccacacc catctcaacc ttcttcagat ttgactttcc aagctttccg aacttcatcc
     4921 gcagcgatga agacgtcgag gtggatgttt tcccaggctt tacgtggaga ctgaggctac
     4981 tgggtctctt agggacgttc tgctgcttag gtaagatggt tataggggtg tccccatgcc
     5041 tactggatcc ttgtgacccc gaaacctcag aaaccatctt catgagagga taaaacgggc
     5101 cgtgggaggc tgacgtttca ggagagcaga actgtttctg tgaatgttcc ataaggctct
     5161 cgtccgagct ctctctcatg ttcctctgga cttcgctcgg gtcgagtttt ggtatctcca
     5221 ggtcttctcg tgtcagctga gcacagagat tggtggccac tggtggagga tgcgtggcat
     5281 tgcaggactc tgagacagga gtagcggagg agctttctgt gccagactgt cgtgattggg
     5341 cctgctgcca ctcatagttg atgcagttct ttttcttctc tgcgggatta gttcctcctg
     5401 ctgaggtttg ctccactgag gtgtcattgt ggagtggttt atcagcacct tcatggtctt
     5461 cagtgtttgt ggatgagtgt tcaaggacag aacctacttt aggtgtctgc agtgcagtgc
     5521 tgtggttaag agcaggactg gctgattttt ctctgtccca gatgaggagc agttcggcga
     5581 gtcgctcctc cgcacactga gctgtcagtc gggcttctgc gtcctgatcc cagcagtctt
     5641 ccatcgtctc tttcagagaa tgcaccgtca ggctattctc tttccaggct tctgggaatt
     5701 tgggtctttc tttttctctg gacacaagcg cctgcacgtc ctctatggta ggatgattgc
     5761 ccacctctgc ctggaatgcc aactgaaaag ctggcactgt ttcacctggg aaaagatctg
     5821 cacagcgcat gaaggtctcc cagtaaacca gacccagtgc ataaacatcc acttgtttca
     5881 gcgcagactc acaatccctc agattcacag ccccttccag cacttctgga gccatgtacc
     5941 gcactgtacc tacctcactg atggcactgt tgtcctcttc tccatgacca ggcggcctct
     6001 ttcctatcag aatcatggac agtccaaagt cactgatcac acacgagccg tccgtcttaa
     6061 ccagcacatt cctgctgttc agatcccggt gggagacagc cggtttatac acatcccctc
     6121 gctgtatctc tgtatgtagg tacgccaacc ctctggtcac agacagagcc aaacggcagc
     6181 agctcaacca gtccacagtc cggccgctca gatacgtgca cagagagcca tgaggataaa
     6241 actccagcag aaggagaaac tctgtccgac cttctgtgcc gacgcgctcc tcgctctcta
     6301 ggaagcgcgc tatgttctcg tgatccagga ggaggcgata aatcatacgc tcattagtga
     6361 actgctggcg gttagcagaa atgaaaactt tcactgctac agatcggtca tccagcgaag
     6421 cacgatacac tgtaccgtac cggccccggc caatcagctc ctgcagtgtg aggttgtcca
     6481 gatccaaaga aggaggagaa agtgccgtct ccagtatatt taaagtatgc agtgagtgtt
     6541 taccacgtcc tcttagcatg cggctacctc cgccaccact tccaccgcct ccagaacctc
     6601 ctccaccgcg ctggctgggg tccttaggct tgctcttgct actccccatg gtggcatcga
     6661 tgggatcctg caaaaagaac aagtagcttg tattctatag tgtcacctaa atcaagcttg
     6721 ctccgacgtc cccaggcaga atggcggttc cctaaacgag cattgcttat atagacctcc
     6781 cattaggcac gcctaccgcc catttacgtc aatggaacgc ccatttgcgt cattgcccct
     6841 ccccattgac gtcaatgggg atgtacttgg cagccatcgc gggccattta ccgccattga
     6901 cgtcaatggg agtactgcca atgtaccctg gcgtacttcc aatagtaatg tacttgccaa
     6961 gttactatta atagatattg atgtactgcc aagtgggcca tttaccgtca ttgacgtcaa
     7021 tagggggcgt gagaacggat atgaatgggc aatgagccat cccattgacg tcaatggtgg
     7081 gtggtcctat tgacgtcaat gggcattgag ccaggcgggc catttaccgt aattgacgtc
     7141 aatgggggag gcgccatata cgtcaatagg accgcccata tga
//
