LOCUS       sequence-409836-        7357 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry-fused Pcdh-gamma-B2 lacking an EC1 domain and an ICD domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7357)
  AUTHORS   Kanadome T, Hoshino N, Nagai T, Yagi T, Matsuda T
  TITLE     Visualization of trans-interactions of a protocadherin-alpha between
            processes originating from single neurons.
  JOURNAL   iScience. 2023 Jul 17;26(7):107238. doi: 10.1016/j.isci.2023.107238.
            eCollection 2023 Jul 21.
  PUBMED    37534169
REFERENCE   2  (bases 1 to 7357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2023-07-17"; volume: "26(7):107238. doi"; pages: " 
            10.1016/j.isci.2023.107238. eCollection 2023 Jul 21"
COMMENT     Sequence Label: pCX:gamma-B2-Delta-EC1-Delta-ICD-mCh
FEATURES             Location/Qualifiers
     source          1..7357
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          662..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3668..4375
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDQMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3810..3828)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4078..4097
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4272..4295
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(4379..4398)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4444..4499
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4498..4517)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4860..4876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4860..4876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4873..4895)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4884..4900
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4908..4914,4915..4932,4933..4938))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4953..4974
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5033..5228
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5079..5214
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5234..5368
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5271..5290)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5325..5344
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5436..5453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5607..6195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5687..5706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6366..7157,7158..7226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6989..7008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7227..7331)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggsgs gccrgggggg gggggggggg gggggggggg
      541 ggggggggsg rggcggrgag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgcgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc
      721 gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc
      781 tccgggctgt aattagcgct tggtttaatg acggcttgtt tcttttctgt ggctgcgtga
      841 aagccttgag gggctccggg agggcccttt gtgcgggggg agcggctcgg ggggtgcgtg
      901 cgtgtgtgtg tgcgtgggga gcgccgcgtg cggctccgcg ctgcccggcg gctgtgagcg
      961 ctgcgggcgc ggcgcggggc tttgtgcgct ccgcagtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg gggggggctg cgaggggaac aaaggctgcg tgcggggtgt
     1081 gtgcgtgggg gggtgagcag ggggtgtggg cgcgtcggtc gggctgcaac cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gkgggggtgc cgggcggggc
     1261 ggggccgcck cgggccgggg rgggctcggg ggrggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccct ctccagcctc ggggctgtcc
     1561 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcgccaccat
     1741 gaggaggggc tctgggagcg gtccggcctg gtggctgcag gtactgtcgc ctttcctgtt
     1801 gcttttgttc cacggagctc tcgcggacaa cgctcctcta ttcaaacaga gtgaggttga
     1861 tttaaacatt ggagagtcca ccagaccagg taaagcattt ccattggatc cagccctcga
     1921 tttagatgct ggttctaatt ctctgcaaaa atactatctc actgacaatg aatactttga
     1981 agtcacggag aaagagaccc cagatggacg taaatatcct gagttgattc taaaacgttt
     2041 tctggacaga gaagagcata attttcacca actggtcctc acggctgtgg atgggggaga
     2101 tcctccccga agtggcacca cccagatccg aatccaagtc acagacatca atgataaccc
     2161 gccaatgttc agccaggatg tgttcagtgt cacccttcgg gaggatgtac caccaggctt
     2221 ctccgtgctt caggtgacag ccaccgacca ggacgagggt gtcaatgcag aaatcaccta
     2281 cgcttttcac aatgtggatg aacaagtgga acggattttt aacttagaca agagaacagg
     2341 agaaatcacg acaaaggata atttggattt cgagacagct aagagttaca ccctgaatgt
     2401 agaagcaaaa gatcccggag acctagcatc ccattgtagt atccaagtga aaattctgga
     2461 tgagaatgat tgtgtaccgg aagtaattgt gacgtcagta tttacccccc taccagaaga
     2521 ttctccacta ggaacagtca tcgccttgat aaaaaccaga gacagagact ctggagaaaa
     2581 tggagacgtc tactgccatg tgttgggaaa tgaagggttt gtattgaaat cttcttcgaa
     2641 gaattattac aaactagtga cagacagaac tctagaccgg gaggcgatac cggaatacaa
     2701 cgtcaccatt gtagctgccg ataggggtaa gcccccgctc tcctctaacg taatcatcac
     2761 tctccatatc tcagatgtca acgacaatgc accggttttt caccaggcct cctacttggt
     2821 ccatgtggct gagaacaacc cacctggaac ctccattgct caagtcagtg cttctgaccc
     2881 ggatttggga tccaatggcc tcatttccta ctccatcata gccagcgacc tagagccaag
     2941 ggcactatcg tccttcgtgt ccgtgaacca ggacagtgga gtggtgttcg cgcagcgcgc
     3001 cttcgaccac gagcagctgc gctccttcca gctgacacta caggcgcggg accacggatc
     3061 gcccacactc agtgccaatg tgagcatgcg tgtgctggtg ggcgaccgca acgacaacgc
     3121 gccacgcgtg ctgtatccca cacttgagcc cgacggctct gcgctcttcg acatggtgcc
     3181 gcgcgctgcc gagcccggat acctggtcac caaggtggtg gctgtggatg cagactccgg
     3241 acacaatgcc tggctatctt accacgtgct gcaggccagc gatcccgggc tcttcagcct
     3301 gggactgcgc acaggcgagg tgcgcacagc gcgtgccctg ggagacaggg actcagcgcg
     3361 gcagcgcctt ctggtcgctg tgcgtgatgg tggacagcca ccgctctccg ctacagccac
     3421 gctgcacctc atcttcgctg acagcctgca ggaggtcctg ccagacctca gcgatgaccc
     3481 tctaccatct gaccctcaga gtgagcttca gttttacctg gtggtggcct tggccctgat
     3541 ctcagtactc ttcctcctgt cggtgattct ggctattgcc ctgcgtctga gacactcctc
     3601 cagatcagct gcctggggct gctttcaacc tggcctcagc tctaaccttg cgactggagt
     3661 tgctagcgtg agcaagggcg aggaggacca gatggccatc atcaaggagt tcatgcgctt
     3721 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
     3781 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gcggccccct
     3841 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
     3901 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
     3961 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
     4021 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
     4081 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
     4141 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
     4201 cgccgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
     4261 cgtgaacatc aagctggaca tcacctccca caacgaggac tacaccatcg tggaacagta
     4321 cgagcgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agtaaagatc
     4381 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     4441 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     4501 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     4561 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     4621 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     4681 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     4741 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     4801 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     4861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     4921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     4981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     5041 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     5101 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     5161 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     5221 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5281 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5341 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     5401 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5461 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5521 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5581 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5641 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5701 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5761 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5821 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5881 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5941 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6001 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     6061 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6121 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6181 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6241 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6301 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6361 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6421 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6481 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6541 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6601 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6661 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6721 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6781 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6841 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6901 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6961 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7021 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7081 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7141 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7201 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7261 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7321 ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
