LOCUS       sequence-409882-       11988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Cassidy JS, Ferron L, Kadurin I, Pratt WS, Dolphin AC
  TITLE     Functional exofacially tagged N-type calcium channels elucidate the 
            interaction with auxiliary alpha2delta-1 subunits.
  JOURNAL   Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8979-84. doi: 
            10.1073/pnas.1403731111. Epub 2014 Jun 2.
  PUBMED    24889613
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1403731111"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2014-06-17- 17"; volume: "111"; issue: "24"; pages: "8979-84"
COMMENT     Sequence Label: Cav2.2 HA pCAGGS
FEATURES             Location/Qualifiers
     source          1..11988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        5..384
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..663
                     /label=chicken beta-actin promoter
     intron          664..1680
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1604..1626
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1688..1707
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3474..3500
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3504..3530
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9010..9029)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9075..9130
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9129..9148)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9491..9507)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9491..9507)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9504..9526)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9515..9531
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9539..9545,9546..9563,9564..9569))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9584..9605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9664..9859
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9710..9845
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    9865..9999
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9902..9921)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9956..9975
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10067..10084)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10238..10826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10318..10337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10997..11788,11789..11857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11620..11639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11858..11962)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat
       61 agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg
      121 cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
      181 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
      241 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
      301 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
      361 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggggcgc gcnncaggcg gggcggggcg gggcgagggg
      541 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
      601 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
      661 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
      721 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
      781 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
      841 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag
      961 cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc
     1021 gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt
     1081 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg
     1141 cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg
     1201 cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg
     1261 gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc ccggagcgcc
     1321 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg
     1381 gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc
     1441 accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg
     1501 gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt
     1561 ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt
     1621 gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag
     1681 ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag
     1741 ggtaccgggc cccccctcga ggtcgaagct tgatcatggt ccgcttcggg gacgagctgg
     1801 gcggccgcta tgggggcgcg ggcggcgcgg agcgggcccg gggcggcggg gccggcgggg
     1861 ctggcggccc gggccccggg gggctgccgc cgggccagcg ggtcctctac aaacagtcga
     1921 tcgcgcagcg cgcgcggacc atggcgctgt acaaccccat cccggtcaag cagaactgct
     1981 tcaccgtcaa ccgctcgctc ttcgtcttca gcgaggacaa cgtcgtccgc aaatacgcca
     2041 agcgcatcac cgagtggcct ccgttcgagt acatgatcct ggccaccatc atcgccaact
     2101 gcatcgtgct ggccctggag cagcacctcc ctgacgggga caagacgcct atgtccgagc
     2161 gcctggatga caccgagccc tacttcattg ggatcttctg cttcgaggcg gggatcaaga
     2221 tcctggccct gggctttgtt ctgcacaagg gctcctatct gcggaacggc tggaacgtca
     2281 tggacttcgt ggtagtcctc acggggattc ttgccacagc cggaaccgac ttcgacctgc
     2341 gaacgctgag ggctgtgcgt gtgctaaggc ccctgaagct ggtgtctggg attccgagct
     2401 tgcaagtggt gctcaagtcc atcatgaagg ccatggtccc gctgctgcag atcgggctgc
     2461 tgctcttctt cgccatcctc atgtttgcca tcatcggcct cgagttctac atgggcaagt
     2521 tccacaaggc ctgcttcccc aacagcacag acccagatcc cgtgggtgac ttcccctgtg
     2581 gcaaggaggc cccggcccgg ctctgtgagg gagacacgga gtgccgggag tactgggccg
     2641 gacccaactt tggtatcacc aactttgaca acatcctgtt tgccatcttg accgtgttcc
     2701 agtgcatcac catggagggc tggactgaca tcctctacaa tacaaacgat gcagccggca
     2761 acacgtggaa ctggctctac ttcatccctc tcatcatcat cggctccttc ttcatgctca
     2821 acctggtgct gggtgtgctc tcaggagagt ttgctaagga gcgcgagaga gtggagaacc
     2881 gccgcgcctt cctgaagctc cggcggcagc agcagattga gcgggagctc aacgggtacc
     2941 tggagtggat cttcaaggct gaggaagtca tgctggctga ggaggacagg aacgcagagg
     3001 agaagtcccc tctggatgca gtgttgaaga gagcagctgc caagaagagc agaagcgacc
     3061 tgatccaggc cgaggagggg gagggccggc tcacaggcct ctgtgctccc ggttccccgt
     3121 tcgcccgtgc cagcctgaaa agcgggaaga cggagagctc gtcgtacttc cggaggaagg
     3181 agaagatgtt ccgcttcttc atccggcgca tggtgaaggc acagagcttc tactggacgg
     3241 tgctgtgtgt ggtggcactg aacacactgt gcgtggccat ggtgcactac aaccagccgc
     3301 agcggctcac cacggcattg tactttgcag agtttgtttt cctgggtctc ttcctcacag
     3361 agatgtccct gaagatgtat ggcctggggc ccaggagcta cttccggtcc tccttcaact
     3421 gttttgactt tggggtcatc gtgggcagta tctttgaagt ggtctgggct gcttacccct
     3481 acgacgtccc cgactacgcc ggctacccgt acgacgtccc ggactacgcc ggcgtcgacg
     3541 tcaagccagg gacctccttc gggatcagtg ttctgcgggc tctgcgctta ctgagaatct
     3601 tcaaggtcac caaatactgg aactccctac ggaacctggt ggtgtctctg ctcaactcca
     3661 tgaagtccat catcagcctc ctcttcctgc tcttcctgtt cattgttgtc tttgctctgc
     3721 tggggatgca gctgtttgga ggccagttca actttaaaga cgagacccca acgaccaact
     3781 ttgacacctt ccctgctgct atcctcaccg tcttccagat cctgaccgga gaggactgga
     3841 atgcggtcat gtaccacggg atcgagtcgc aaggtggcgt cagcagagga atgttctcct
     3901 ccttctactt cattgtcctc actctgtttg ggaactacac cctgctgaat gtcttcctgg
     3961 ccattgctgt ggacaacctt gccaatgctc aggagctgac caaggatgaa gaggagatgg
     4021 aagaagcagc caatcagaaa ctcgccttgc agaaggccaa ggaagtggct gaagtcagcc
     4081 ccatgtctgc tgcgaacatc tccattgccg ccaggcagca gaactcggcc aaagcgcgct
     4141 cggtgtggga gcagcgagcc agccagctgc ggctgcagaa cctgagggcc agctgcgagg
     4201 cgctgtacag cgagatggac cccgaggaga ggctgcgcta cgccaccgcg cgccacctgc
     4261 ggcccgacgt gaagacgcac ctggaccggc cgctggtggt tgagcccggc cgcgacgctc
     4321 cgcggggccc cccggggggg aagtcccggc cggatggctc ggaggccccc gagggtgcgg
     4381 atcccccgcg caggcatcac cggcaccgag acaaggacaa ggctcccgcc acggtcccct
     4441 cggcggggga acaggacaga gccgaagctc tgagggcaga gggcggggag ctggggcccc
     4501 gcgaggagcg ggggcggcca cgccgcagcc gcagcaagga ggccccgggc gccccggagg
     4561 tgcgcagcga tcgcggccgt ggcccgtgtc ctgaaggtgg tcggcgccac caccggcgcg
     4621 ggtcaccgga ggaggcagct gagagggagc cccgccgcca ccgcgcgcac cggcacgggc
     4681 ccgacccggg caaggagggc ccagcgtccg ggaccagggg cgagcggcgc gcacgacacc
     4741 gcacgggccc ccgggcgtgc ccccgggagg ctgagagcag cgaggagcct gcgcggcggc
     4801 accgggctcg gcacaaggcg ccgcccaccc aggagaccgc ggagaaggac aaggaggctg
     4861 ccgagaaggg gggcgaggcc acggaggccg agaaggacaa ggaggctcgg aaccaccagc
     4921 ccaaggagct gccctgtgac ctggaggcca tcggcatgct gggagtgggc gctgtgcata
     4981 cgctgcccag cacctgtctg cagaaggtgg aggagcagcc agaggatgcg gacaaccagc
     5041 ggaacgtcac ccgcatgggt agtcagcccc cggacaccag caccactgtg cacattcctg
     5101 tgacgctgac cggccccccc ggggagacca cggtagtgcc cagtggaaac gtggacctgg
     5161 aaagtcaagc agaggggaag aaggaggtgg aaaccagcga tgtgatgagg agcggccctc
     5221 ggcccattgt cccgtacagt tccatgttct gtttaagccc aaccaacctg ctgcgccgct
     5281 gctgccacta cattgtgacc atgcgatact tcgagatggt catcctcgtg gtcatcgccc
     5341 tgagcagcat cgccctggct gccgaggacc ccgtgagaac agactctccc aggaataatg
     5401 ctctgaagta catggactac atcttcacag gcgtcttcac cttcgagatg gtgataaaga
     5461 tgatcgattt gggactgctg ctacaccctg gagcctattt ccgggatctg tggaacattc
     5521 tggacttcat tgtggtcagt ggggccctgg tggcatttgc tttctcagga tccaaaggga
     5581 aagacatcag caccatcaaa tccctgagag tcctgcgtgt gctgcggcca ctcaagacca
     5641 tcaagcggct gcccaagctc aaggccgtgt ttgactgtgt ggtgaactca ctgaaaaacg
     5701 tcctcaacat cctgatcgtc tacatgctct tcatgttcat ctttgccgtc attgccgtgc
     5761 agctcttcaa agggaagttc ttctactgca cggacgaatc caaggagctg gagcgtgact
     5821 gcaggggtca gtatttggat tatgagaagg aggaagtgga agctcaaccg aggcagtgga
     5881 agaaatatga cttccactat gacaacgtgc tctgggctct gctaacgctc ttcacggtgt
     5941 ccacagggga agggtggccc atggtgctga aacactctgt ggatgccacc tacgaggagc
     6001 agggcccgag ccccgggtac cgcatggagc tctccatctt ctacgtggtc tactttgtgg
     6061 tctttccctt cttctttgtc aacatcttcg tggccttgat catcatcacc ttccaggagc
     6121 agggggacaa ggtgatgtcc gagtgcagcc tggagaagaa cgagagggct tgcatcgact
     6181 ttgccatcag cgccagaccc ctgacgcggt acatgcccca gaacaagcag tcgttccagt
     6241 ataagacgtg gacgttcgtg gtgtccccac cttttgagta cttcatcatg gctatgatcg
     6301 ccctcaacac tgtggtgctg atgatgaagt tctacgatgc gccgtacgag tatgagctaa
     6361 tgctgaaatg cctgaacatc gtgttcacgt ccatgttctc catggagtgt gtgctgaaga
     6421 tcatcgcctt cggggtgctg aactatttca gagatgcctg gaatgtcttt gactttgtca
     6481 cggtgttggg aagtattact gatattttgg taacagagat tgcgaacaac ttcatcaacc
     6541 tcagcttcct gcgcctgttc cgggccgcgc ggctcatcaa gctgctgcgc cagggctaca
     6601 ccatccgcat cctgctttgg accttcgtgc agtccttcaa ggcgctgccc tacgtgtgtc
     6661 tcctcatcgc catgctcttc ttcatctacg ccatcattgg catgcaggtt tttgggaaca
     6721 tcgccctgga cgatgacacc agcatcaaca ggcacaataa cttccggacg tttctgcagg
     6781 ccttgatgtt gctgttcagg agcgccacgg gggaggcctg gcacgagata atgctgtcgt
     6841 gcctcagcag ccgggcctgc gacgagcact ccaatgccag cgagtgcggc agcgactttg
     6901 cctactttta cttcgtctcc ttcatcttcc tctgctcctt tctgatgttg aacctctttg
     6961 tggctgtgat catggacaat tttgagtacc ttacacggga ctcttccatc ttagggcctc
     7021 accacctgga tgagttcatc cgggtctggg ctgaatacga cccggccgcg tgtgggcgca
     7081 tcagttacag tgacatgttt gagatgctga agcacatgtc cccacccctg gggctgggga
     7141 agaagtgccc tgctcgagtc gcgtacaagc gcctggtccg catgaacatg cccatctcca
     7201 gtgaggacat gacggtgcac ttcacatcca cgctgatggc tctcattcgg acagcgctgg
     7261 acatcaagct ggcaccagct gggaccaagc agcaccagtg cgacgcagag ctgaggaagg
     7321 agatttcctg tgtgtgggcc aatctgcccc agaagaccct ggacttactg gtaccacccc
     7381 ataagcctga cgagatgacg gtgggcaaag tctatgcagc tctgatgatc ttcgacttct
     7441 acaaacagaa caaaaccagc agagaccaga ctcagcaagc ccccggaggc ctgtcccagc
     7501 tgggccccgt gtccctgttc cacccgctga aggccacgct ggagcagact cagccggcgc
     7561 tgcgtggagc ccgggctttc ctgcggcaga agagttctgc ctccctgagc aacggcgggg
     7621 ccgtacaaac ccaggagagc ggcatcaagg aatcggtttc ttggggcact cagaggaccc
     7681 aggatgtgct ttgcgaggcc agggcacccc tggagcgggg ccactcagct gagatcccag
     7741 tcgggcagcc tgggacactg gctgtagatg tccagatgca gaacatgact ctgagtggcc
     7801 ctgacgccga gccgcagcca gggctggaga gccagggccg agccgcctcc atgccccgcc
     7861 tggccgcaga aacacagccc gcgcctgacg ccagccccat gaagcgctcc atctccacgc
     7921 tggccccgcg cccccacacg gcccggctcg gcagcaccgc gctggaccgc ccagccccca
     7981 gccaggcccc gcaccatcac caccaccgct gccaccgccg cagggacagg aagcagcggt
     8041 ccctggagaa gggtcccagc ctgtctgctg acacagatgg ggcccccgac agcacggtgg
     8101 ggccaggact gcccacgggg gagggccccc ccggctgcag gcgggagcgg gagcgcaggc
     8161 aggagcgggg ccggtcccag gagcggaggc agccctcgtc ctcctcctcg gagaagcatc
     8221 gcttctactc ctgtgaccgc ttcgggggcc gcgaaccccc acagcccaag ccctccctca
     8281 gtagtcaccc cacgtcgccg acggccgggc aggagccggg accccacccg caggggagtg
     8341 gttcggttca cgggagcccc ctgctgtcca catctggtgc tagcaccccg ggccgggggc
     8401 ggaggcagct cccccagacc cccttgaccc cccgccccag cgtcacctac aagacggcca
     8461 actcctcgcc tgtccacttc gccggggcac cgagcggcct ccccgccttc tcccccggcc
     8521 ggctcagccg gggcctctca gaacacaacg ccctgctgca gagggacccg ctcagccggc
     8581 ccctggcgcc cggctcgcgc atcggctccg acccctacct ggggcagcgg ctggacagcg
     8641 aggcgcccgc ccgtgccctg cccgaggacg cgcccgcctt tgaggagacc gcggccagca
     8701 actcgggccg ctcctcccgg acctcgtacg tgtcctcact gacctcgcag cccccgccgc
     8761 tgcgccgcgt gcccaacggc taccactgca ctctggggct gggcgggggc ggccgggcgc
     8821 ggcgcggctg ccaccacccc gaccgcgacc gccggtgcta gactagttct agcaagcttc
     8881 gacgaattcg catgcggccg ctagctcgag gtaccgatat cgagctcaat tcactcctca
     8941 ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc aatgccctgg ctcacaaata
     9001 ccactgagat ctttttccct ctgccaaaaa ttatggggac atcatgaagc cccttgagca
     9061 tctgacttct ggctaataaa ggaaatttat tttcattgca atagtgtgtt ggaatttttt
     9121 gtgtctctca ctcggaagga catatgggag ggcaaatcat ttaaaacatc agaatgagta
     9181 tttggtttag agtttggcaa catatgccca tatgctggct gccatgaaca aaggttggct
     9241 ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat tccatagaaa
     9301 agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt tttctttaac
     9361 atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct cctgactact
     9421 cccagtcata gctgtccctc ttctcttatg gagatccctc gacctgcagc ccaagcttgg
     9481 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     9541 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     9601 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga
     9661 tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     9721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     9781 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     9841 aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg ttacaaataa
     9901 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     9961 ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa tgaatcggcc
    10021 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10081 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10141 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10201 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10261 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10321 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10381 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10441 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10501 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10561 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    10621 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    10681 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    10741 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    10801 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    10861 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    10921 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    10981 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11041 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11101 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11161 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11221 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11281 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11341 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11401 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11461 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11521 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    11581 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    11641 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    11701 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    11761 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    11821 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    11881 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11941 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
