LOCUS       sequence-409921-        9910 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of the FRET/BRET cAMP sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   French AR, Tesmer AL, Tantama M
  TITLE     Dual-Mode FRET and BRET Sensors for Detecting cAMP Dynamics.
  JOURNAL   ACS Omega. 2019 Sep 12;4(13):15504-15511. doi: 
            10.1021/acsomega.9b01770. eCollection 2019 Sep 24.
  PUBMED    31572851
REFERENCE   2  (bases 1 to 9910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acsomega.9b01770"; journalName: "ACS Omega"; date: 
            "2019-09-12- 12"; volume: "4"; issue: "13"; pages: "15504-15511"
COMMENT     Sequence Label: GW1-mScarlet-Epac-GeNL
FEATURES             Location/Qualifiers
     source          1..9910
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     6..25
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     257..279
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     271..288
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     272..288
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        854..1233
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1234..1437
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1388..1408
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1434..1458
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1573..2399
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             2605..3300
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(2738..2756)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3006..3025
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      5767..5776
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6819..6953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6856..6875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6910..6929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6976..6992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6976..6992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6989..7011)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7000..7016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7024..7030,7031..7048,7049..7054))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7206..7223)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7377..7965)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7457..7476)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8136..8927,8928..8996))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8759..8778
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      complement(9053..9566)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9248..9269)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9460..9479
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9738..9756
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9794..9816)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgaagacgt cgcctcctca
       61 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
      121 tagtcagggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
      181 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
      241 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc ccgtgtcaag
      301 gacggtgact gcagtgaata ataaaatgtg tgtttgtccg aaatacgcgt tttgagattt
      361 ctgtcgccga ctaaattcat gtcgcgcgat agtggtgttt atcgccgata gagatggcga
      421 tattggaaaa atcgatattt gaaaatatgg catattgaaa atgtcgccga tgtgagtttc
      481 tgtgtaactg atatcgccat ttttccaaaa gtgatttttg ggcatacgcg atatctggcg
      541 atagcgctta tatcgtttac gggggatggc gatagacgac tttggtgact tgggcgattc
      601 tgtgtgtcgc aaatatcgca gtttcgatat aggtgacaga cgatatgagg ctatatcgcc
      661 gatagaggcg acatcaagct ggcacatggc caatgcatat cgatctatac attgaatcaa
      721 tattggccat tagccatatt attcattggt tatatagcat aaatcaatat tggctattgg
      781 ccattgcata cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca
      841 ttaccgccat gttgacattg attattgact agttattaat agtaatcaat tacggggtca
      901 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      961 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     1021 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     1081 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     1141 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     1201 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     1261 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     1321 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     1381 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
     1441 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
     1501 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
     1561 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcttat
     1621 gcatgctata ctgtttttgg cttggggtct atacaccccc gcttcctcat gttataggtg
     1681 atggtatagc ttagcctata ggtgtgggtt attgaccatt attgaccact cccctattgg
     1741 tgacgatact ttccattact aatccataac atggctcttt gccacaactc tctttattgg
     1801 ctatatgcca atacactgtc cttcagagac tgacacggac tctgtatttt tacaggatgg
     1861 ggtctcattt attatttaca aattcacata tacaacacca ccgtccccag tgcccgcagt
     1921 ttttattaaa cataacgtgg gatctccacg cgaatctcgg gtacgtgttc cggacatggg
     1981 ctcttctccg gtagcggcgg agcttctaca tccgagccct gctcccatgc ctccagcgac
     2041 tcatggtcgc tcggcagctc cttgctccta acagtggagg ccagacttag gcacagcacg
     2101 atgcccacca ccaccagtgt gccgcacaag gccgtggcgg tagggtatgt gtctgaaaat
     2161 gagctcgggg agcgggcttg caccgctgac gcatttggaa gacttaaggc agcggcagaa
     2221 gaagatgcag gcagctgagt tgttgtgttc tgataagagt cagaggtaac tcccgttgcg
     2281 gtgctgttaa cggtggaggg cagtgtagtc tgagcagtac tcgttgctgc cgcgcgcgcc
     2341 accagacata atagctgaca gactaacaga ctgttccttt ccatgggtct tttctgcagt
     2401 caccgtcctt gacacgaagc ttcccggcca cagcaatcga tccatgacac aaccagtgac
     2461 aagcagtccc ccagtgaaac cgcatccgca tccgagtccg ataccgataa agattctgaa
     2521 tcggagtgag tgccgcgtcc gagagcgttc cctgtccacg tccaccatcg gcggagcagg
     2581 tgtgcctgag gcctctagac caccatggtg agcaagggcg aggcagtgat caaggagttc
     2641 atgcggttca aggtgcacat ggagggctcc atgaacggcc acgagttcga gatcgagggc
     2701 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     2761 ggccccctgc ccttctcctg ggacatcctg tcccctcagt tcatgtacgg ctccagggcc
     2821 ttcaccaagc accccgccga catccccgac tactataagc agtccttccc cgagggcttc
     2881 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgccg tgaccgtgac ccaggacacc
     2941 tccctggagg acggcaccct gatctacaag gtgaagctcc gcggcaccaa cttccctcct
     3001 gacggccccg taatgcagaa gaagacaatg ggctgggaag cgtccaccga gcggttgtac
     3061 cccgaggacg gcgtgctgaa gggcgacatt aagatggccc tgcgcctgaa ggacggcggc
     3121 cgctacctgg cggacttcaa gaccacctac aaggccaaga agcccgtgca gatgcccggc
     3181 gcctacaacg tcgaccgcaa gttggacatc acctcccaca acgaggacta caccgtggtg
     3241 gaacagtacg aacgctccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     3301 gctggtaccc ccgtgggaac tcatgagatg gaggaggagt tggccgaagc tgtggccctg
     3361 ctctcccagc gggggcctga cgccctgctc actgtggcac ttcgaaagcc cccaggtcag
     3421 cgcacggatg aagagctgga cctcatcttt gaggagctgc tgcacatcaa ggctgtggcc
     3481 cacctctcca actcggtgaa gcgagaatta gcggctgttc tgctctttga accacacagc
     3541 aaggcaggga ccgtgttgtt cagccagggg gacaagggca cttcgtggta cattatctgg
     3601 aagggatctg tcaacgtggt gacccatggc aaggggctgg tgaccaccct gcatgaggga
     3661 gatgattttg gacagctggc tctggtgaat gatgcacccc gggcagccac catcatcctg
     3721 cgagaagaca actgtcattt cctgcgtgtg gacaagcagg acttcaaccg tatcatcaag
     3781 gatgtggagg caaagaccat gcggctggaa gaacatggca aagtggtgct ggtgctggag
     3841 agagcctctc agggcgccgg cccttcccga cccccaaccc caggcaggaa ccggtataca
     3901 gtgatgtctg gcaccccaga gaagatccta gagcttctgt tggaggccat gggaccagat
     3961 tccagtgctc atgaccaaac agagacattc ctcagcgact tcctcctgac ccacagggtc
     4021 ttcatgccca gcgcccaact ctgcgctgcc cttctgcacc acttccatgt ggagcctgcg
     4081 ggtggcagcg agcaggagcg cagcacctac gtctgcaaca agaggcagca gatcttgcgg
     4141 ctggtcagcc agtgggtggc cctgtatggc tccatgctcc acactgaccc tgtggccacc
     4201 agcttcctcc agaaactctc agacctggtg ggcagggaca cccgactcag caacctgctg
     4261 agggagcagt ggccagagag gcggcgatgc cacaggttgg agaatggctg tgggaatgca
     4321 tctcctcaga tgaaggcccg gaacttgcct gtttggctcc ccaaccagga cgagcccctt
     4381 cctggcagca gctgtgccat ccaagttggg gataaagtcc cctatgacat ctgccggcca
     4441 gaccactcag tgttgaccct gcagctgcct gtgacagcct ccgtgagaga ggtgatggca
     4501 gcgttggccc aggaggatgg ctggaccaag gggcaggtgc tggtgaaggt caattctgca
     4561 ggtgatgcca ttggcctgca gccagatgcc cgtggtgtgg ccacatctct ggggctcaat
     4621 gagcgtctct ttgttgtcaa cccacaggaa gcgcatgagc tgatcccaca ccctgaccag
     4681 ctggggccca ctgtgggctc tgctgagggg ctggacctgg tgagtgccaa ggacctggca
     4741 ggccagctga cggaccacga ctggagcctc ttcaacagta tccaccaggt ggagctgatc
     4801 cactatgtgc tgggccccca gcatctgcgg gatgtcacca ccgccaacct ggagcgcttc
     4861 atgcgccgct tcaatgagct gcagtactgg gtggccaccg agctgtgtct ctgccccgtg
     4921 cccggccccc gggcccagct gctcaggaag ttcattaagc tggcggccca cctcaaggag
     4981 cagaagaatc tcaattcctt ctttgccgtc atgtttggcc tcagcaactc ggccatcagc
     5041 cacctagccc acacctggga gcggctgccc cacaaagtcc ggaagctgta ctccgccctc
     5101 gagaggctgc tggacccctc atggaaccac cgggtatacc gactggccct cgccaagctc
     5161 tcccctcctg tcatcccctt catgcccctt cttctcaaag acatgacctt cattcatgag
     5221 ggaaaccaca cactagtgga gaatctcatc aactttgaga agatgagaat gatggccaga
     5281 gtcgcgcgga tgctgcacca ctgccgaagc cacaaccctg tgcctctctc accactcaga
     5341 agccgagttt cccacctcca cgaggacagc caggtggcga ggatttccac atgctcggag
     5401 cagtccctga gcacccggag tccagccagc acctgggctt atgtccagca gctgaaggtc
     5461 attgacaacc agcgggaact ctcccgcctc tcccgagagc tggagccaga gcaagtcagc
     5521 aagggcgagg aggataacat ggcctctctc ccagcgacac atgagttaca catctttggc
     5581 tccatcaacg gtgtggactt tgacatggtg ggtcagggca ccggcaatcc aaatgatggt
     5641 tatgaggagt taaacctgaa gtccaccaag ggtgacctcc agttctcccc ctggattctg
     5701 gtccctcata tcgggtatgg cttccatcag tacctgccct accctgacgg gatgtcgcct
     5761 ttccaggccg ccatggtaga tggctccggc taccaagtcc atcgcacaat gcagtttgaa
     5821 gatggtgcct cccttactgt taactaccgc tacacctacg agggaagcca catcaaagga
     5881 gaggcccagg tgaaggggac tggtttccct gctgacggtc ctgtgatgac caactcgctg
     5941 accgctgcgg actggtgcag gtcgaagaag acttacccca acgacaaaac catcatcagt
     6001 acctttaagt ggagttacac cactggaaat ggcaagcgct accggagcac tgcgcggacc
     6061 acctacacct ttgccaagcc aatggcggct aactatctga agaaccagcc gatgtacgtg
     6121 ttccgtaaga cggagctcaa gcactccaag accgagctca acttcaagga gtggcaaaag
     6181 gcgttcaccg gcttcgaaga tttcgttggg gactggcgac agacagccgg ctacaacctg
     6241 gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg ggtgtccgta
     6301 actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga catccatgtc
     6361 atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa aatttttaag
     6421 gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg cacactggta
     6481 atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga aggcatcgcc
     6541 gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa caaaattatc
     6601 gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat caacggagtg
     6661 accggctggc ggctgtgcga acgcattctg gcctaaactc gacgaattca ttgatcataa
     6721 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     6781 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     6841 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6901 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtaccg
     6961 agctcgcgta atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     7021 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     7081 actacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     7141 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     7201 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7261 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7321 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7381 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7441 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7501 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7561 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7621 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7681 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7741 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7801 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7861 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7921 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7981 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8041 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8101 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8161 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8221 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8281 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8341 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8401 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8461 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8521 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8581 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8641 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8701 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8761 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8821 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8881 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8941 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     9001 ttcctttttc aataagcggc cgcggccatg ccggccacta gtctcgaggg ccaccccggt
     9061 tgataatcag aaaagcccca aaaacaggaa gattgtataa gcaaatattt aaattgtaag
     9121 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     9181 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     9241 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     9301 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     9361 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     9421 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     9481 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     9541 gcttaatgcg ccgctacagg gcgcgtgggg atccctcgag ttattattga agcatttatc
     9601 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     9661 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     9721 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     9781 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     9841 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     9901 gctggcttaa
//
