LOCUS       sequence-409942-       13302 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 with a N-terminal GFP tag and an 
            exofacial double HA tag in domain II and a L702P mutation that 
            allows trafficking to the plasma membrane but channel is not 
            functional.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13302)
  TITLE     Dolphin Lab Plasmids 2023
REFERENCE   2  (bases 1 to 13302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GFP Cav2.2 HA L702P pcDNA3
FEATURES             Location/Qualifiers
     source          1..13302
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2..19)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(173..761)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(253..272)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(932..1723,1724..1792))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1555..1574
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1793..1897)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1972..1991)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2163..2542
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2543..2746
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2697..2717
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2791..2810
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2791..2809
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2901..3614
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             2901..3614
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2931..2959)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3554..3574
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             5313..5339
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5343..5369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(10783..10801)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10784..10801)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10821..10838)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10827..11051
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11097..11525
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11184..11203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11394..11415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11534..11554)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11539..11868
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11719..11854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11781..11800
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             11935..12729
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11989..12008)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12599..12618
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    12903..13024
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12940..12959)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12994..13013
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13073..13089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13073..13089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13086..13108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13097..13113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13121..13127,13128..13145,13146..13151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13166..13187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
       61 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      121 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      181 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      241 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      301 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      361 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
      421 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
      481 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
      541 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
      601 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
      661 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
      721 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
      781 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
      841 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
      901 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
      961 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1021 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1081 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1141 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1201 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1261 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1321 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1381 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     1441 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     1501 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     1561 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     1621 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     1681 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     1741 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     1801 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     1861 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     1921 ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca gtacaatctg
     1981 ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg aggtcgctga
     2041 gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca attgcatgaa
     2101 gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca gatatacgcg
     2161 ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag
     2221 cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
     2281 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
     2341 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
     2401 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
     2461 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
     2521 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
     2581 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
     2641 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
     2701 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctct ggctaactag
     2761 agaacccact gcttactggc ttatcgaaat taatacgact cactataggg agacccaagc
     2821 ttggtaccga gctcggatcc actagtaacg gccgccagtg tgctggaatt cctcgagggt
     2881 accgagctcg gatctaaaaa atgagtaaag gagaagaact tttcactgga gttgtcccaa
     2941 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
     3001 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
     3061 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
     3121 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
     3181 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
     3241 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
     3301 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
     3361 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
     3421 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
     3481 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
     3541 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
     3601 atgaactata caaaatggtc cgcttcgggg acgagctggg cggccgctat gggggcgcgg
     3661 gcggcgcgga gcgggcccgg ggcggcgggg ccggcggggc tggcggcccg ggccccgggg
     3721 ggctgccgcc gggccagcgg gtcctctaca aacagtcgat cgcgcagcgc gcgcggacca
     3781 tggcgctgta caaccccatc ccggtcaagc agaactgctt caccgtcaac cgctcgctct
     3841 tcgtcttcag cgaggacaac gtcgtccgca aatacgccaa gcgcatcacc gagtggcctc
     3901 cgttcgagta catgatcctg gccaccatca tcgccaactg catcgtgctg gccctggagc
     3961 agcacctccc tgacggggac aagacgccta tgtccgagcg cctggatgac accgagccct
     4021 acttcattgg gatcttctgc ttcgaggcgg ggatcaagat cctggccctg ggctttgttc
     4081 tgcacaaggg ctcctatctg cggaacggct ggaacgtcat ggacttcgtg gtagtcctca
     4141 cggggattct tgccacagcc ggaaccgact tcgacctgcg aacgctgagg gctgtgcgtg
     4201 tgctaaggcc cctgaagctg gtgtctggga ttccgagctt gcaagtggtg ctcaagtcca
     4261 tcatgaaggc catggtcccg ctgctgcaga tcgggctgct gctcttcttc gccatcctca
     4321 tgtttgccat catcggcctc gagttctaca tgggcaagtt ccacaaggcc tgcttcccca
     4381 acagcacaga cccagatccc gtgggtgact tcccctgtgg caaggaggcc ccggcccggc
     4441 tctgtgaggg agacacggag tgccgggagt actgggccgg acccaacttt ggtatcacca
     4501 actttgacaa catcctgttt gccatcttga ccgtgttcca gtgcatcacc atggagggct
     4561 ggactgacat cctctacaat acaaacgatg cagccggcaa cacgtggaac tggctctact
     4621 tcatccctct catcatcatc ggctccttct tcatgctcaa cctggtgctg ggtgtgctct
     4681 caggagagtt tgctaaggag cgcgagagag tggagaaccg ccgcgccttc ctgaagctcc
     4741 ggcggcagca gcagattgag cgggagctca acgggtacct ggagtggatc ttcaaggctg
     4801 aggaagtcat gctggctgag gaggacagga acgcagagga gaagtcccct ctggatgcag
     4861 tgttgaagag agcagctgcc aagaagagca gaagcgacct gatccaggcc gaggaggggg
     4921 agggccggct cacaggcctc tgtgctcccg gttccccgtt cgcccgtgcc agcctgaaaa
     4981 gcgggaagac ggagagctcg tcgtacttcc ggaggaagga gaagatgttc cgcttcttca
     5041 tccggcgcat ggtgaaggca cagagcttct actggacggt gctgtgtgtg gtggcactga
     5101 acacactgtg cgtggccatg gtgcactaca accagccgca gcggctcacc acggcattgt
     5161 actttgcaga gtttgttttc ctgggtctct tcctcacaga gatgtccctg aagatgtatg
     5221 gcctggggcc caggagctac ttccggtcct ccttcaactg ttttgacttt ggggtcatcg
     5281 tgggcagtat ctttgaagtg gtctgggctg cttaccccta cgacgtcccc gactacgccg
     5341 gctacccgta cgacgtcccg gactacgccg gcgtcgacgt caagccaggg acctccttcg
     5401 ggatcagtgt tctgcgggct ctgcgcttac tgagaatctt caaggtcacc aaatactgga
     5461 actccctacg gaacctggtg gtgtctctgc tcaactccat gaagtccatc atcagcctcc
     5521 tcttcctgct cttcctgttc attgttgtct ttgctctgct ggggatgcag ctgtttggag
     5581 gccagttcaa ctttaaagac gagaccccaa cgaccaactt tgacaccttc cctgctgcta
     5641 tcctcaccgt cttccagatc ctgaccggag aggactggaa tgcggtcatg taccacggga
     5701 tcgagtcgca aggtggcgtc agcagaggaa tgttctcctc cttctacttc attgtcctca
     5761 ctctgtttgg gaactacacc ctgccgaatg tcttcctggc cattgctgtg gacaaccttg
     5821 ccaatgctca ggagctgacc aaggatgaag aggagatgga agaagcagcc aatcagaaac
     5881 tcgccttgca gaaggccaag gaagtggctg aagtcagccc catgtctgct gcgaacatct
     5941 ccattgccgc caggcagcag aactcggcca aagcgcgctc ggtgtgggag cagcgagcca
     6001 gccagctgcg gctgcagaac ctgagggcca gctgcgaggc gctgtacagc gagatggacc
     6061 ccgaggagag gctgcgctac gccaccgcgc gccacctgcg gcccgacgtg aagacgcacc
     6121 tggaccggcc gctggtggtt gagcccggcc gcgacgctcc gcggggcccc ccggggggga
     6181 agtcccggcc ggatggctcg gaggcccccg agggtgcgga tcccccgcgc aggcatcacc
     6241 ggcaccgaga caaggacaag gctcccgcca cggtcccctc ggcgggggaa caggacagag
     6301 ccgaagctct gagggcagag ggcggggagc tggggccccg cgaggagcgg gggcggccac
     6361 gccgcagccg cagcaaggag gccccgggcg ccccggaggt gcgcagcgat cgcggccgtg
     6421 gcccgtgtcc tgaaggtggt cggcgccacc accggcgcgg gtcaccggag gaggcagctg
     6481 agagggagcc ccgccgccac cgcgcgcacc ggcacgggcc cgacccgggc aaggagggcc
     6541 cagcgtccgg gaccaggggc gagcggcgcg cacgacaccg cacgggcccc cgggcgtgcc
     6601 cccgggaggc tgagagcagc gaggagcctg cgcggcggca ccgggctcgg cacaaggcgc
     6661 cgcccaccca ggagaccgcg gagaaggaca aggaggctgc cgagaagggg ggcgaggcca
     6721 cggaggccga gaaggacaag gaggctcgga accaccagcc caaggagctg ccctgtgacc
     6781 tggaggccat cggcatgctg ggagtgggcg ctgtgcatac gctgcccagc acctgtctgc
     6841 agaaggtgga ggagcagcca gaggatgcgg acaaccagcg gaacgtcacc cgcatgggta
     6901 gtcagccccc ggacaccagc accactgtgc acattcctgt gacgctgacc ggcccccccg
     6961 gggagaccac ggtagtgccc agtggaaacg tggacctgga aagtcaagca gaggggaaga
     7021 aggaggtgga aaccagcgat gtgatgagga gcggccctcg gcccattgtc ccgtacagtt
     7081 ccatgttctg tttaagccca accaacctgc tgcgccgctg ctgccactac attgtgacca
     7141 tgcgatactt cgagatggtc atcctcgtgg tcatcgccct gagcagcatc gccctggctg
     7201 ccgaggaccc cgtgagaaca gactctccca ggaataatgc tctgaagtac atggactaca
     7261 tcttcacagg cgtcttcacc ttcgagatgg tgataaagat gatcgatttg ggactgctgc
     7321 tacaccctgg agcctatttc cgggatctgt ggaacattct ggacttcatt gtggtcagtg
     7381 gggccctggt ggcatttgct ttctcaggat ccaaagggaa agacatcagc accatcaaat
     7441 ccctgagagt cctgcgtgtg ctgcggccac tcaagaccat caagcggctg cccaagctca
     7501 aggccgtgtt tgactgtgtg gtgaactcac tgaaaaacgt cctcaacatc ctgatcgtct
     7561 acatgctctt catgttcatc tttgccgtca ttgccgtgca gctcttcaaa gggaagttct
     7621 tctactgcac ggacgaatcc aaggagctgg agcgtgactg caggggtcag tatttggatt
     7681 atgagaagga ggaagtggaa gctcaaccga ggcagtggaa gaaatatgac ttccactatg
     7741 acaacgtgct ctgggctctg ctaacgctct tcacggtgtc cacaggggaa gggtggccca
     7801 tggtgctgaa acactctgtg gatgccacct acgaggagca gggcccgagc cccgggtacc
     7861 gcatggagct ctccatcttc tacgtggtct actttgtggt ctttcccttc ttctttgtca
     7921 acatcttcgt ggccttgatc atcatcacct tccaggagca gggggacaag gtgatgtccg
     7981 agtgcagcct ggagaagaac gagagggctt gcatcgactt tgccatcagc gccagacccc
     8041 tgacgcggta catgccccag aacaagcagt cgttccagta taagacgtgg acgttcgtgg
     8101 tgtccccacc ttttgagtac ttcatcatgg ctatgatcgc cctcaacact gtggtgctga
     8161 tgatgaagtt ctacgatgcg ccgtacgagt atgagctaat gctgaaatgc ctgaacatcg
     8221 tgttcacgtc catgttctcc atggagtgtg tgctgaagat catcgccttc ggggtgctga
     8281 actatttcag agatgcctgg aatgtctttg actttgtcac ggtgttggga agtattactg
     8341 atattttggt aacagagatt gcgaacaact tcatcaacct cagcttcctg cgcctgttcc
     8401 gggccgcgcg gctcatcaag ctgctgcgcc agggctacac catccgcatc ctgctttgga
     8461 ccttcgtgca gtccttcaag gcgctgccct acgtgtgtct cctcatcgcc atgctcttct
     8521 tcatctacgc catcattggc atgcaggttt ttgggaacat cgccctggac gatgacacca
     8581 gcatcaacag gcacaataac ttccggacgt ttctgcaggc cttgatgttg ctgttcagga
     8641 gcgccacggg ggaggcctgg cacgagataa tgctgtcgtg cctcagcagc cgggcctgcg
     8701 acgagcactc caatgccagc gagtgcggca gcgactttgc ctacttttac ttcgtctcct
     8761 tcatcttcct ctgctccttt ctgatgttga acctctttgt ggctgtgatc atggacaatt
     8821 ttgagtacct tacacgggac tcttccatct tagggcctca ccacctggat gagttcatcc
     8881 gggtctgggc tgaatacgac ccggccgcgt gtgggcgcat cagttacagt gacatgtttg
     8941 agatgctgaa gcacatgtcc ccacccctgg ggctggggaa gaagtgccct gctcgagtcg
     9001 cgtacaagcg cctggtccgc atgaacatgc ccatctccag tgaggacatg acggtgcact
     9061 tcacatccac gctgatggct ctcattcgga cagcgctgga catcaagctg gcaccagctg
     9121 ggaccaagca gcaccagtgc gacgcagagc tgaggaagga gatttcctgt gtgtgggcca
     9181 atctgcccca gaagaccctg gacttactgg taccacccca taagcctgac gagatgacgg
     9241 tgggcaaagt ctatgcagct ctgatgatct tcgacttcta caaacagaac aaaaccagca
     9301 gagaccagac tcagcaagcc cccggaggcc tgtcccagct gggccccgtg tccctgttcc
     9361 acccgctgaa ggccacgctg gagcagactc agccggcgct gcgtggagcc cgggctttcc
     9421 tgcggcagaa gagttctgcc tccctgagca acggcggggc cgtacaaacc caggagagcg
     9481 gcatcaagga atcggtttct tggggcactc agaggaccca ggatgtgctt tgcgaggcca
     9541 gggcacccct ggagcggggc cactcagctg agatcccagt cgggcagcct gggacactgg
     9601 ctgtagatgt ccagatgcag aacatgactc tgagtggccc tgacgccgag ccgcagccag
     9661 ggctggagag ccagggccga gccgcctcca tgccccgcct ggccgcagaa acacagcccg
     9721 cgcctgacgc cagccccatg aagcgctcca tctccacgct ggccccgcgc ccccacacgg
     9781 cccggctcgg cagcaccgcg ctggaccgcc cagcccccag ccaggccccg caccatcacc
     9841 accaccgctg ccaccgccgc agggacagga agcagcggtc cctggagaag ggtcccagcc
     9901 tgtctgctga cacagatggg gcccccgaca gcacggtggg gccaggactg cccacggggg
     9961 agggcccccc cggctgcagg cgggagcggg agcgcaggca ggagcggggc cggtcccagg
    10021 agcggaggca gccctcgtcc tcctcctcgg agaagcatcg cttctactcc tgtgaccgct
    10081 tcgggggccg cgaaccccca cagcccaagc cctccctcag tagtcacccc acgtcgccga
    10141 cggccgggca ggagccggga ccccacccgc aggggagtgg ttcggttcac gggagccccc
    10201 tgctgtccac atctggtgct agcaccccgg gccgggggcg gaggcagctc ccccagaccc
    10261 ccttgacccc ccgccccagc gtcacctaca agacggccaa ctcctcgcct gtccacttcg
    10321 ccggggcacc gagcggcctc cccgccttct cccccggccg gctcagccgg ggcctctcag
    10381 aacacaacgc cctgctgcag agggacccgc tcagccggcc cctggcgccc ggctcgcgca
    10441 tcggctccga cccctacctg gggcagcggc tggacagcga ggcgcccgcc cgtgccctgc
    10501 ccgaggacgc gcccgccttt gaggagaccg cggccagcaa ctcgggccgc tcctcccgga
    10561 cctcgtacgt gtcctcactg acctcgcagc ccccgccgct gcgccgcgtg cccaacggct
    10621 accactgcac tctggggctg ggcgggggcg gccgggcgcg gcgcggctgc caccaccccg
    10681 accgcgaccg ccggtgctag actagttcta gcaagcttcg acgaattctg cagatatcca
    10741 tcacactggc ggccgctcga gcatgcatct agagggccct attctatagt gtcacctaaa
    10801 tgctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
    10861 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
    10921 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
    10981 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
    11041 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
    11101 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
    11161 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
    11221 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
    11281 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
    11341 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
    11401 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
    11461 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
    11521 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
    11581 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    11641 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
    11701 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    11761 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
    11821 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
    11881 gcttgtatat ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt
    11941 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
    12001 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
    12061 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
    12121 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
    12181 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
    12241 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
    12301 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
    12361 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
    12421 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
    12481 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
    12541 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
    12601 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
    12661 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
    12721 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
    12781 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
    12841 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
    12901 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    12961 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    13021 cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc
    13081 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    13141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    13201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    13261 gcgcggggag aggcggtttg cgtattgggc gctcttccgc tt
//
