LOCUS       sequence-409982-        9809 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SREBP-1 (isoform 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, 
            Loncarek J, Tsai YC, Weissman AM
  TITLE     SARS-CoV-2 papain-like protease plays multiple roles in regulating 
            cellular proteins in the endoplasmic reticulum.
  JOURNAL   J Biol Chem. 2023 Oct 12;299(12):105346. doi: 
            10.1016/j.jbc.2023.105346.
  PUBMED    37838170
REFERENCE   2  (bases 1 to 9809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2023-10-12"; volume: "299(12):105346. doi"; pages: " 
            10.1016/j.jbc.2023.105346"
COMMENT     Sequence Label: pCDNA3-SREBP-1-GFP (A317-318; A341)
FEATURES             Location/Qualifiers
     source          1..9809
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(50..70)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        55..384
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      235..370
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     297..316
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             451..1245
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(505..524)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1115..1134
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1419..1540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1456..1475)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1510..1529
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1589..1605)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1589..1605)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1602..1624)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1613..1629
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1637..1643,1644..1661,1662..1667))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1682..1703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1820..1837)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1991..2579)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2071..2090)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2750..3541,3542..3610))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3373..3392
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3611..3715)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3790..3809)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3981..4360
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4361..4564
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4515..4535
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4609..4628
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4609..4627
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4653..4662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8277..8303
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8310..8336
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8343..8369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(8385..8387,8388..9101)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8385..8387,8388..9098)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8688..8707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9035..9056
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9146..9163)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9152..9376
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9422..9809,1..41)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9509..9528)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9719..9740
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
       61 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      121 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      181 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
      241 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
      301 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
      361 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
      421 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
      481 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
      541 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
      601 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
      661 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
      721 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
      781 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
      841 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
      901 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
      961 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
     1021 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
     1081 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
     1141 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
     1201 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
     1261 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
     1321 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     1381 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     1441 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1501 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga
     1561 cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     1621 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     1681 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     1741 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     1801 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     1861 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     1921 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     1981 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     2041 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     2101 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     2161 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     2221 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     2281 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     2341 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     2401 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     2461 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     2521 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     2581 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     2641 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     2701 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     2761 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     2821 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     2881 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     2941 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3001 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3061 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3121 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     3181 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     3241 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     3301 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     3361 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     3421 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     3481 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     3541 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     3601 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     3661 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     3721 ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg
     3781 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg
     3841 tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt
     3901 gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt
     3961 acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac
     4021 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     4081 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     4141 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     4201 tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa
     4261 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac
     4321 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     4381 catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     4441 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa
     4501 ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     4561 agctctctgg ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca
     4621 ctatagggag acccaagctg gctagcggat ccgccaccat ggcctctatg accggcggcc
     4681 agcaaatggg tatggcctct atgaccggcg gccagcaaat gggagcatcg atggacgagc
     4741 cacccttcag cgaggcggct ttggagcagg cgctgggcga gccgtgcgat ctggacgcgg
     4801 cgctgctgac cgacatcgaa ggtgaagtcg gcgcggggag gggtagggcc aacggcctgg
     4861 acgccccaag ggcgggcgca gatcgcggag ctatggattg cactttcgaa gacatgcttc
     4921 agcttatcaa caaccaagac agtgacttcc ctggcctatt tgacccaccc tatgctggga
     4981 gtggggcagg gggcacagac cctgccagcc ccgataccag ctccccaggc agcttgtctc
     5041 cacctcctgc cacattgagc tcctctcttg aagccttcct gagcgggccg caggcagcgc
     5101 cctcacccct gtcccctccc cagcctgcac ccactccatt gaagatgtac ccgtccatgc
     5161 ccgctttctc ccctgggcct ggtatcaagg aagagtcagt gccactgagc atcctgcaga
     5221 cccccacccc acagcccctg ccaggggccc tcctgccaca gagcttccca gccccagccc
     5281 caccgcagtt cagctccacc cctgtgttag gctaccccag ccctccggga ggcttctcta
     5341 caggaagccc tcccgggaac acccagcagc cgctgcctgg cctgccactg gcttccccgc
     5401 caggggtccc gcccgtctcc ttgcacaccc aggtccagag tgtggtcccc cagcagctac
     5461 tgacagtcac agctgccccc acggcagccc ctgtaacgac cactgtgacc tcgcagatcc
     5521 agcaggtccc ggtcctgctg cagccccact tcatcaaggc agactcgctg cttctgacag
     5581 ccatgaagac agacggagcc actgtgaagg cggcaggtct cagtcccctg gtctctggca
     5641 ccactgtgca gacagggcct ttgccgaccc tggtgagtgc cgcaaccatc ttggcaacag
     5701 tcccactggt cgtagatgcg gagaagctgc ctatcaaccg gctcgcagct gccagcaagg
     5761 ccccggcctc tgcccagagc cgtggagaga agcgcacagc ccacaacgcc attgagaagc
     5821 gctaccgctc ctccatcaat gacaaaatca ttgagctcaa ggatctggtg gtgggcactg
     5881 aggcaaagct gaataaatct gctgtcttgc gcaaggccat cgactacatt cgctttctgc
     5941 aacacagcaa ccagaagctt aagcaggaga acctaagtct gcgcactgct gtccacaaaa
     6001 gcaaatctct gaaggatctg gtgtcggcct gtggcagtgg agggaacaca gacgtgctca
     6061 tggagggcgt gaagactgag gtggaggaca cactgacccc acccccctcg gatgctggct
     6121 cacctttcca gagcagcccc ttgtcccttg gcagcagggg cagtggcagc ggtggcagtg
     6181 gcagtgactc ggagcctgac agcccagtct ttgaggacag caaggcaaag ccagagcagc
     6241 ggccgtctct gcacagccgc ggcatgctgg accgctcccg cctggccctg tgcacgctcg
     6301 tcttcctctg cctgtcctgc aaccccttgg cctccttgct gggggcccgg gggcttccca
     6361 gcccctcaga taccaccagc gtctaccata gccctgggcg caacgtgctg ggcaccgagt
     6421 ctagagatgg ccctggctgg gcccagtggc tgctgccccc agtggtctgg ctgctcaatg
     6481 ggctgttggt gctcgtctcc ttggtgcttc tctttgtcta cggtgagcca gtcacacggc
     6541 cccactcagg ccccgccgtg tacttctgga ggcatcgcaa gcaggctgac ctggacctgg
     6601 cccggggaga ctttgcccag gctgcccagc agctgtggct ggccctgcgg gcactgggcc
     6661 ggcccctgcc cacctcccac ctggacctgg cttgtagcct cctctggaac ctcatccgtc
     6721 acctgctgca gcgtctctgg gtgggccgct ggctggcagg ccgggcaggg ggcctgcagc
     6781 aggactgtgc tctgcgagtg gatgcaagcg ccagcgcccg agacgcagcc ctggtctacc
     6841 ataagctgca ccagctgcac accatgggga agcacacagg cgggcacctc actgccacca
     6901 acctggcgct gagtgccctg aacctggcag agtgtgcagg ggatgccgtg tctgtggcga
     6961 cgctggccga gatctatgtg gcggctgcat tgagagtgaa gaccagtctc ccacgggcct
     7021 tgcattttct gacacgcttc ttcctgagca gtgcccgcca ggcctgcctg gcacagagtg
     7081 gctcagtgcc tcctgccatg cagtggctct gccaccccgt gggccaccgt ttcttcgtgg
     7141 atggggactg gtccgtgctc agtaccccat gggagagcct gtacagcttg gccgggaacc
     7201 cagtggaccc cctggcccag gtgactcagc tattccggga acatctctta gagcgagcac
     7261 tgaactgtgt gacccagccc aaccccagcc ctgggtcagc tgatggggac aaggaatttt
     7321 cggatgccct cgggtacctg cagctgctga acagctgttc tgatgctgcg ggggctcctg
     7381 cctacagctt ctccatcagt tccagcatgg ccaccaccac cggcgtagac ccggtggcca
     7441 agtggtgggc ctctctgaca gctgtggtga tccactggct gcggcgggat gaggaggcgg
     7501 ctgagcggct gtgcccgctg gtggagcacc tgccccgggt gctgcaggag tctgagagac
     7561 ccctgcccag ggcagctctg cactccttca aggctgcccg ggccctgctg ggctgtgcca
     7621 aggcagagtc tggtccagcc agcctgacca tctgtgagaa ggccagtggg tacctgcagg
     7681 acagcctggc taccacacca gccagcagct ccattgacaa ggccgtgcag ctgttcctgt
     7741 gtgacctgct tcttgtggtg cgcaccagcc tgtggcggca gcagcagccc ccggccccgg
     7801 ccccagcagc ccagggcacc agcagcaggc cccaggcttc cgcccttgag ctgcgtggct
     7861 tccaacggga cctgagcagc ctgaggcggc tggcacagag cttccggccc gccatgcgga
     7921 gggtgttcct acatgaggcc acggcccggc tgatggcggg ggccagcccc acacggacac
     7981 accagctcct cgaccgcagt ctgaggcggc gggcaggccc cggtggcaaa ggaggcgcgg
     8041 tggcggagct ggagccgcgg cccacgcggc gggagcacgc ggaggccttg ctgctggcct
     8101 cctgctacct gccccccggc ttcctgtcgg cgcccgggca gcgcgtgggc atgctggctg
     8161 aggcggcgcg cacactagag aaacttggcg atcgccggct gctgcacgac tgtcagcaga
     8221 tgctcatgcg cctgggcggt gggaccactg tcacttccag cggagaattc ggatcatacc
     8281 catacgatgt tccagattac gctagtggct atccttatga cgtacctgat tatgcaggca
     8341 gttacccata cgatgtacca gactacgctg gtaccgtatc gggcgtgagc aagggcgagg
     8401 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     8461 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     8521 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     8581 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     8641 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     8701 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     8761 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     8821 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     8881 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     8941 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     9001 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     9061 ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgct cgagtctagt
     9121 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     9181 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     9241 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     9301 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     9361 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     9421 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     9481 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     9541 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     9601 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9661 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9721 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9781 taagggattt tgccgatttc ggcctattg
//
