LOCUS       sequence-409986-       11682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integration-free (episomal) expression of human ePOU and shRNA 
            against p53..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11682)
  TITLE     unpublished plasmid for non-intregating (episomal) expression of 
            ePOU
REFERENCE   2  (bases 1 to 11682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pCXLE_hePOU_shp53
FEATURES             Location/Qualifiers
     source          1..11682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..662
                     /label=chicken beta-actin promoter
     intron          663..1679
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1603..1625
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1687..1706
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     misc_feature    2867..3455
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2920..2940)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3338..3349)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3546..3565)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3611..3666
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3665..3684)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4027..4043)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4027..4043)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4040..4062)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4051..4067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4075..4081,4082..4099,4100..4105))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4120..4141
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    4230..4263
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             4724..6649
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGXGGGXGXGGGXGGXGGXGGGGGXGGGGGXGGXGXGGGA
                     GGXGGAGXGGGAGAGGGAGGAGAGGGXGGXGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGXGGAGAGGAGAGGAGGAGAGGAGGXGXGGXGGXGXGGGXGGXGXG
                     GGXGGXGXGGXGGXGXGGGGGXGXGGXGGAGXGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    6817..6850
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      7037..8826
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     promoter        9296..9609
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     9528..9548
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     complement(9760..9777)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9931..10519)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10011..10030)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10690..11481,11482..11550))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11313..11332
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11551..11655)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggggggcgcg cgccgggggg gggggggggg gggggggggc
      541 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
      601 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
      661 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
      841 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt
      901 gcgtgtgtgt gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg
     1021 ggggcggtgc cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg
     1081 tgtgcgtggg ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc
     1141 acccccctcc ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc
     1201 gtggcgcggg gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg
     1261 cggggccgcc tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg
     1321 gcggctgtcg aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg
     1381 cgcagggact tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca
     1441 ccccctctag cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg
     1501 agggccttcg tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc
     1561 cgcgggggga cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg
     1621 tgaccggcgg ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc
     1681 tcctgggcaa cgtgctggtt attgtgctgt ctcatcattt tggcaaagaa ttcgcccttc
     1741 accatggcgg gacacctggc ttcggatttc gccttctcgc cccctccagg tggtggaggt
     1801 gatgggccag gggggccgga gccgggctgg gttgatcctc ggacctggct aagcttccaa
     1861 ggccctcctg gagggccagg aatcgggccg ggggttgggc caggctctga ggtgtggggg
     1921 attcccccat gccccccgcc gtatgagttc tgtgggggga tggcgtactg tgggccccag
     1981 gttggagtgg ggctagtgcc ccaaggcggc ttggagacct ctcagcctga gggcgaagca
     2041 ggagtcgggg tggagagcaa ctccgatggg gcctccccgg agccctgcac cgtcacccct
     2101 ggtgccgtga agctggagaa ggagaagctg gagcaaaacc cggaggagtc ccaggacatc
     2161 aaagctctgc agaaagaact cgagcaattt gccaagctcc tgaagcagaa gaggatcaga
     2221 ctgggatata cacaggccga tgtggggctc accctggggg ttctatttgg gaaggtattc
     2281 agccaaacga ccatctgccg ctttgaggct ctgcagctta gcttcaagaa catgtgtaag
     2341 ctgcggccct tgctgcagaa gtgggtggag gaagctgaca acaatccaaa tcttcaggag
     2401 atatgcaaag cagaaaccct cgtgcaggcc cgaaagagaa agcgaaccag tatcgaggtg
     2461 agcgtcaagg gggctctgga gagccatttc ctcaaatgcc ccaagccctc ggcccaggag
     2521 atcacctccc tcgcggacag cttacagctg gagaaggagg tggtgagagt gtggttctgt
     2581 aaccggcgcc agaagggcaa gcgatcaagc agcgactatg cacaacgaga ggattttgag
     2641 gctgctgggt ctcctttctc agggggacca gtgtcctttc ctctggcccc agggccccat
     2701 tttggtaccc caggctatgg gagccctcac ttcactgcac tgtactcctc ggtccctttc
     2761 cctgaggggg aagcctttcc ccctgtctct gtcaccactc tgggctctcc catgcattca
     2821 aactgaggta agggcgaatt caagcttcgg ggactagtca tatgataatc aacctctgga
     2881 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2941 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     3001 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     3061 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     3121 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     3181 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     3241 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     3301 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     3361 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     3421 gacgagtcgg atctcccttt gggccgcctc cccgcatcgg tacaattcac tcctcaggtg
     3481 caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca caaataccac
     3541 tgagatcttt ttccctctgc caaaaattat ggggacatca tgaagcccct tgagcatctg
     3601 acttctggct aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt
     3661 ctctcactcg gaaggacata tgggagggca aatcatttaa aacatcagaa tgagtatttg
     3721 gtttagagtt tggcaacata tgcccatatg ctggctgcca tgaacaaagg ttggctataa
     3781 agaggtcatc agtatatgaa acagccccct gctgtccatt ccttattcca tagaaaagcc
     3841 ttgacttgag gttagatttt ttttatattt tgttttgtgt tatttttttc tttaacatcc
     3901 ctaaaatttt ccttacatgt tttactagcc agatttttcc tcctctcctg actactccca
     3961 gtcatagctg tccctcttct cttatggaga tccctcgacc tgcagcccaa gcttggcgta
     4021 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     4081 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     4141 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agcggatctc
     4201 aattccgatc atattcaata acccttaata taacttcgta taatgtatgc tatacgaagt
     4261 tattaggtct gaagaggagt ttacgtccag ccaagcttag gatcaattct catgtttgac
     4321 agcttatcat cgataagctg atcctcacag gccgcaccca gcttttcttc cgttgcccca
     4381 gtagcatctc tgtctggtga ccttgaagag gaagaggagg ggtcccgaga atccccatcc
     4441 ctaccgtcca gcaaaaaggg ggacgaggaa tttgaggcct ggcttgaggc tcaggacgca
     4501 aatcttgagg atgttcagcg ggagttttcc gggctgcgag taattggtga tgaggacgag
     4561 gatggttcgg aggatgggga attttcagac ctggatctgt ctgacagcga ccatgaaggg
     4621 gatgagggtg ggggggctgt tggagggggc aggagtctgc actccctgta ttcactgagc
     4681 gtcgtctaat aaagatgtct attgatctct tttagtgtga atcatgtctg acgaggggcc
     4741 aggtacagga cctggaaatg gcctaggaga gaagggagac acatctggac cagaaggctc
     4801 cggcggcagt ggacctcaaa gaagaggggg tgataaccat ggacgaggac ggggaagagg
     4861 acgaggacga ggaggcggaa gaccaggagc cccgggcggc tcaggatcag ggccaagaca
     4921 tagagatggt gtccggagac cccaaaaacg tccaagttgc attggctgca aagggaccca
     4981 cggtggaaca ggagcaggag caggagcggg aggggcagga gcaggagggg caggagcagg
     5041 aggaggggca ggagcaggag gaggggcagg aggggcagga ggggcaggag gggcaggags
     5101 aggaggaggg gsaggagsag gaggaggggs aggaggggsa ggaggggsag gaggaggagg
     5161 aggggsagga ggaggaggag gggsaggagg ggsaggagsa ggaggagggg caggaggggs
     5221 aggaggggca ggagsaggag gaggggcagg agcaggagga ggggcaggag gggcaggagc
     5281 aggaggaggg gsaggagggg saggaggggc aggagcagga ggaggggcag gagcaggagg
     5341 ggcaggaggg gcaggagggg caggagcagg aggggcagga gcaggaggag gggcaggagg
     5401 ggcaggaggg gcaggagcag gaggggcagg agsaggaggg gcaggagcag gaggggcagg
     5461 agcaggaggg gcaggagggg caggagcagg aggggcagga ggggsaggag saggaggggs
     5521 aggaggggsa ggagsaggag gaggggsagg aggggsagga gsaggaggag gggsaggagg
     5581 ggsaggagsa ggaggggsag gaggggsagg agsaggaggg ggaggagggg saggagsagg
     5641 aggggsagga ggggcaggag saggaggagg ggcaggagca ggaggggcag gagcaggagg
     5701 tggaggccgg ggtcgaggag gcagtggagg ccggggtcga ggaggtagtg gaggccgggg
     5761 tcgaggaggt agtggaggcc gccggggtag aggacgtgaa agagccaggg ggggaagtcg
     5821 tgaaagagcc agggggagag gtcgtggacg tggagaaaag aggcccagga gtcccagtag
     5881 tcagtcatca tcatccgggt ctccaccgcg caggccccct ccaggtagaa ggccattttt
     5941 ccaccctgta ggggaagccg attattttga ataccaccaa gaaggtggcc cagatggtga
     6001 gcctgacgtg cccccgggag cgatagagca gggccccgca gatgacccag gagaaggccc
     6061 aagcactgga ccccggggtc agggtgatgg aggcaggcgc aaaaaaggag ggtggtttgg
     6121 aaagcatcgt ggtcaaggag gttccaaccc gaaatttgag aacattgcag aaggtttaag
     6181 agctctcctg gctaggagtc acgtagaaag gactaccgac gaaggaactt gggtcgccgg
     6241 tgtgttcgta tatggaggta gtaagacctc cctttacaac ctaaggcgag gaactgccct
     6301 tgctattcca caatgtcgtc ttacaccatt gagtcgtctc ccctttggaa tggcccctgg
     6361 acccggccca caacctggcc cgctaaggga gtccattgtc tgttatttca tggtcttttt
     6421 acaaactcat atatttgctg aggttttgaa ggatgcgatt aaggaccttg ttatgacaaa
     6481 gcccgctcct acctgcaata tcagggtgac tgtgtgcagc tttgacgatg gagtagattt
     6541 gcctccctgg tttccaccta tggtggaagg ggctgccgcg gagggtgatg acggagatga
     6601 cggagatgaa ggaggtgatg gagatgaggg tgaggaaggg caggagtgat gtaacttgtt
     6661 aggagacgcc ctcaatcgta ttaaaagccg tgtattcccc cgcactaaag aataaatccc
     6721 cagtagacat catgcgtgct gttggtgtat ttctggccat ctgtcttgtc accattttcg
     6781 tcctcccaac atggggcaat tgccggaacc cttaatataa cttcgtataa tgtatgctat
     6841 acgaagttat taggtccctc gaagaggttc actagcggat ctcaattggg catacccatg
     6901 ttgtcacgtc actcagctcc gcgctcaaca ccttctcgcg ttggaaaaca ttagcgacat
     6961 ttacctggtg agcaatcaga catgcgacgg ctttagcctg gcctccttaa attcacctaa
     7021 gaatgggagc aaccagcagg aaaaggacaa gcagcgaaaa ttcacgcccc cttgggaggt
     7081 ggcggcatat gcaaaggata gcactcccac tctactactg ggtatcatat gctgactgta
     7141 tatgcatgag gatagcatat gctacccgga tacagattag gatagcatat actacccaga
     7201 tatagattag gatagcatat gctacccaga tatagattag gatagcctat gctacccaga
     7261 tataaattag gatagcatat actacccaga tatagattag gatagcatat gctacccaga
     7321 tatagattag gatagcctat gctacccaga tatagattag gatagcatat gctacccaga
     7381 tatagattag gatagcatat gctatccaga tatttgggta gtatatgcta cccagatata
     7441 aattaggata gcatatacta ccctaatctc tattaggata gcatatgcta cccggataca
     7501 gattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
     7561 gattaggata gcctatgcta cccagatata aattaggata gcatatacta cccagatata
     7621 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
     7681 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca tggcaacatt
     7741 agcccaccgt gctctcagcg acctcgtgaa tatgaggacc aacaaccctg tgcttggcgc
     7801 tcaggcgcaa gtgtgtgtaa tttgtcctcc agatcgcagc aatcgcgccc ctatcttggc
     7861 ccgcccacct acttatgcag gtattccccg gggtgccatt agtggttttg tgggcaagtg
     7921 gtttgaccgc agtggttagc ggggttacaa tcagccaagt tattacaccc ttattttaca
     7981 gtccaaaacc gcagggcggc gtgtgggggc tgacgcgtgc ccccactcca caatttcaaa
     8041 aaaaagagtg gccacttgtc tttgtttatg ggccccattg gcgtggagcc ccgtttaatt
     8101 ttcgggggtg ttagagacaa ccagtggagt ccgctgctgt cggcgtccac tctctttccc
     8161 cttgttacaa atagagtgta acaacatggt tcacctgtct tggtccctgc ctgggacaca
     8221 tcttaataac cccagtatca tattgcacta ggattatgtg ttgcccatag ccataaattc
     8281 gtgtgagatg gacatccagt ctttacggct tgtccccacc ccatggattt ctattgttaa
     8341 agatattcag aatgtttcat tcctacacta gtatttattg cccaaggggt ttgtgagggt
     8401 tatattggtg tcatagcaca atgccaccac tgaacccccc gtccaaattt tattctgggg
     8461 gcgtcacctg aaaccttgtt ttcgagcacc tcacatacac cttactgttc acaactcagc
     8521 agttattcta ttagctaaac gaaggagaat gaagaagcag gcgaagattc aggagagttc
     8581 actgcccgct ccttgatctt cagccactgc ccttgtgact aaaatggttc actaccctcg
     8641 tggaatcctg accccatgta aataaaaccg tgacagctca tggggtggga gatatcgctg
     8701 ttccttagga cccttttact aaccctaatt cgatagcata tgcttcccgt tgggtaacat
     8761 atgctattga attagggtta gtctggatag tatatactac tacccgggaa gcatatgcta
     8821 cccgtttagg gttaacaagg gggccttata aacactattg ctaatgccct cttgagggtc
     8881 cgcttatcgg tagctacaca ggcccctctg attgacgttg gtgtagcctc ccgtagtctt
     8941 cctgggcccc tgggaggtac atgtccccca gcattggtgt aagagcttca gccaagagtt
     9001 acacataaag gcaatgttgt gttgcagtcc acagactgca aagtctgctc caggatgaaa
     9061 gccactcagt gttggcaaat gtgcacatcc atttataagg atgtcaacta cagtcagaga
     9121 acccctttgt gtttggtccc cccccgtgtc acatgtggaa cagggcccag ttggcaagtt
     9181 gtaccaacca actgaaggga ttacatgcac tgccccgcga agaaggggca gagatgtcgt
     9241 agtcaggttt agttcgtccg gggcggggat cgatcctcta gagtcgacgc tagcggatcc
     9301 gacgccgcca tctctaggcc cgcgccggcc ccctcgcaca gacttgtggg agaagctcgg
     9361 ctactcccct gccccggtta atttgcatat aatatttcct agtaactata gaggcttaat
     9421 gtgcgataaa agacagataa tctgttcttt ttaatactag ctacatttta catgataggc
     9481 ttggatttct ataagagata caaatactaa attattattt taaaaaacag cacaaaagga
     9541 aactcaccct aactgtaaag taattgtgtg ttttgagact ataaatatcc cttggagaaa
     9601 agccttgttt gggccgactc cagtggtaat ctacttcaag agagtagatt accactggag
     9661 tcttttttgg aattcctgca gcccggggga tccgctgcat taatgaatcg gccaacgcgc
     9721 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     9781 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     9841 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     9901 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     9961 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
    10021 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
    10081 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
    10141 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
    10201 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
    10261 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
    10321 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
    10381 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
    10441 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
    10501 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
    10561 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
    10621 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
    10681 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
    10741 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
    10801 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
    10861 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
    10921 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
    10981 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
    11041 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
    11101 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
    11161 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
    11221 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
    11281 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
    11341 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
    11401 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
    11461 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
    11521 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
    11581 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
    11641 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gg
//
