LOCUS       sequence-409994-       11421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus
            generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11421)
  AUTHORS   Tan DS, Chen Y, Gao Y, Bednarz A, Wei Y, Malik V, Ho DH, Weng M, Ho 
            SY, Srivastava Y, Velychko S, Yang X, Fan L, Kim J, Graumann J, 
            Stormo GD, Braun T, Yan J, Scholer HR, Jauch R
  TITLE     Directed Evolution of an Enhanced POU Reprogramming Factor for Cell 
            Fate Engineering.
  JOURNAL   Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 
            10.1093/molbev/msab075.
  PUBMED    33720298
REFERENCE   2  (bases 1 to 11421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biol Evol"; date: "2021-06-25- 25"; volume: "38"; issue: "7"; 
            pages: "2854-2868"
COMMENT     Sequence Label: pLVTHM_mePOU_3xFlag
FEATURES             Location/Qualifiers
     source          1..11421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2147..3325
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2378..3316
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3273..3293
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3373..3490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4594..4659
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4677..5265
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4730..4750)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5148..5159)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5433..5451,5452..5474,5475..5493,5494..5516,5517..5535,
                     5536..5558,5559..5577,5578..5600,5601..5619,5620..5642,
                     5643..5661,5662..5684,5685..5703)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5732..5946
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     5867..5886
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(5984..6017)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6037..6217
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6242..6260)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6243..6260)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6550..6569)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6783..6802)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6902..6924
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6962..6980)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7048..7152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7153..7221,7222..8013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7371..7390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8184..8772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8673..8692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8926..8943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9013..9033)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9018..9347
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9198..9333
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10481..10546
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10676..10696
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11121..11255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11158..11177)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11212..11231
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcga tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa
     2101 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2161 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2221 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2281 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2341 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2401 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2461 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2521 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2581 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2641 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2701 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2761 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2821 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2881 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2941 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3001 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3061 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3121 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3181 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3241 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3301 ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg
     3361 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     3421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     3481 tcaaaatttt atcgatcacg agactagcct cgaggtttaa acatggctgg acacctggct
     3541 tcagacttcg ccttctcacc cccaccaggt gggggtgatg ggtcagcagg gctggagccg
     3601 ggctgggtgg atcctcgaac ctggctaagc ttccaagggc ctccaggtgg gcctggaatc
     3661 ggaccaggct cagaggtatt ggggatctcc ccatgtccgc ccgcatacga gttctgcgga
     3721 gggatggcat actgtggacc tcaggttgga ctgggcctag tcccccaagt tggcgtggag
     3781 actttgcagc ctgagggcca ggcaggagca cgagtggaaa gcaactcaga gggaacctcc
     3841 tctgagccct gtgccgaccg ccccaatgcc gtgaagttgg agaaggtgga accaactccc
     3901 gaggagtccc aggacatgaa agccctgcag aaggagctag aacagttcgc taagctgctg
     3961 aagcagaaga ggatcaggtt ggggtacacc caggccgacg tggggctcac cctgggcgtt
     4021 ctctttggaa aggtgttcag ccagaccacc atctgtcgct tcgaggcctt gcagctcagc
     4081 cttaagaaca tgtgtaagct gcggcccctg ctggagaagt gggtggagga agccgacaac
     4141 aatccgaacc ttcaggagat atgcaaatcg gagaccctgg tgcaggcccg gaagagaaag
     4201 cgaacgtcca tcgaggtgag cgtcaagggg gctctggaga gccatttcct caaatgccct
     4261 aagccctcgg cccaggagat cacctccctc gcggacagct tacagctgga gaaggaggtg
     4321 gtgcgagtat ggttctgtaa caggcgccag aagggcaaaa gatcaagtat tgagtattcc
     4381 caacgagaag agtatgaggc tacagggaca cctttcccag ggggggctgt atcctttcct
     4441 ctgcccccag gtccccactt tggcacccca ggctatggaa gcccccactt caccacactc
     4501 tactcagtcc cttttcctga gggcgaggcc tttccctctg ttcccgtcac tgctctgggc
     4561 tctcccatgc attcaaacac gcgtggcagc ggcgactaca aggaccacga cggcgactac
     4621 aaggaccacg acatcgacta caaggacgac gacgacaagt agactagtca tatgataatc
     4681 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     4741 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     4801 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     4861 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     4921 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     4981 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     5041 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     5101 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     5161 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     5221 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcgg tacgtatggc
     5281 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5341 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatgg gatcaattca
     5401 ccatgctagt ggatccccct cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5461 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     5521 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     5581 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     5641 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5701 agaaaagtga aagtcggggc tgcaggaatt cgaacgctga cgtcatcaac ccgctccaag
     5761 gaatcgcggg cccagtgtca ctaggcggga acacccagcg cgcgtgcgcc ctggcaggaa
     5821 gatggctgtg agggacaggg gagtggcgcc ctgcaatatt tgcatgtcgc tatgtgttct
     5881 gggaaatcac cataaacgtg aaatgtcttt ggatttggga atcttataag ttctgtatga
     5941 gaccacgcgg cgtctccaag cttatcgata ccgtcgcatg ggaataactt cgtatagcat
     6001 acattatacg aagttatgct gctttttgct tgtactgggt ctctctggtt agaccagatc
     6061 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6121 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6181 ctcagaccct tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta
     6241 ttctatagtg tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc
     6301 cgcgttctaa cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga
     6361 ccctatcatc tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag
     6421 tttctggtaa cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg
     6481 ctagccagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     6541 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     6601 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
     6661 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
     6721 tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc
     6781 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     6841 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     6901 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     6961 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7021 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7081 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7141 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7201 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7261 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7321 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7381 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7441 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7501 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7561 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7621 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7681 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7741 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     7801 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     7861 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     7921 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     7981 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8041 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8101 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8161 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8221 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8281 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8341 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8401 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8461 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8521 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8581 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8641 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8701 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     8761 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     8821 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8881 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8941 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9001 taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
     9061 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     9121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     9181 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     9241 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     9301 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca
     9361 agacaggctt gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt
     9421 gcgtaaaaag acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat
     9481 ctcgcgcaac ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca
     9541 cttcacatga gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa
     9601 ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc
     9661 ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt
     9721 gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag
     9781 gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg
     9841 cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc
     9901 gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt
     9961 gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg
    10021 cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt
    10081 ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt
    10141 aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg
    10201 cagtcggccc ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg
    10261 tggatctggc gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg
    10321 agcgatgccg aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc
    10381 aactggattt atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac
    10441 ataattggac aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag
    10501 tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg
    10561 aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga
    10621 agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa
    10681 aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag
    10741 tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa
    10801 agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca
    10861 taacagttat aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc
    10921 tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa
    10981 taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg
    11041 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
    11101 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
    11161 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
    11221 ccaaactcat caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa
    11281 atcatcccaa acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt
    11341 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    11401 tatgtactac aaacttagta g
//
