LOCUS       sequence-409996-        5665 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mouse ePOU in mammalian cells, for retrovirus generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5665)
  AUTHORS   Tan DS, Chen Y, Gao Y, Bednarz A, Wei Y, Malik V, Ho DH, Weng M, Ho 
            SY, Srivastava Y, Velychko S, Yang X, Fan L, Kim J, Graumann J, 
            Stormo GD, Braun T, Yan J, Scholer HR, Jauch R
  TITLE     Directed Evolution of an Enhanced POU Reprogramming Factor for Cell 
            Fate Engineering.
  JOURNAL   Mol Biol Evol. 2021 Jun 25;38(7):2854-2868. doi: 
            10.1093/molbev/msab075.
  PUBMED    33720298
REFERENCE   2  (bases 1 to 5665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Mol Biol Evol"; date: "2021-06-25- 25"; volume: "38"; issue: "7"; 
            pages: "2854-2868"
COMMENT     Sequence Label: pMX_mePOU
FEATURES             Location/Qualifiers
     source          1..5665
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(934..1725,1726..1794))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1557..1576
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1795..1899)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1929..2522
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2585..2942
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3001..3417
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3347..3369
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3385..3401
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3427..3801
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3739..3755
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     LTR             5077..5546
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt gcggccggcc gcaaatcaat
      901 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      961 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1021 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1081 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1141 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1201 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1261 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1321 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1381 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1441 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1501 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1561 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1621 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1681 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1741 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1801 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1861 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1921 acctgcataa tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt
     1981 tgcaaggcat ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca
     2041 gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc
     2101 tcagggccaa gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
     2161 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
     2221 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     2281 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     2341 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gattgactga
     2401 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
     2461 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
     2521 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
     2581 aggtaagctg gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt
     2641 ttatgcgcct gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg
     2701 gaactgacga gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg
     2761 gccgtttttg tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca
     2821 cccccctaat aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg
     2881 cctccgtctg aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc
     2941 tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaattag
     3001 ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg
     3061 atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca
     3121 gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc
     3181 acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc
     3241 tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta
     3301 caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct
     3361 cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc
     3421 atatggccat atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct
     3481 gacatgacaa gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta
     3541 gtccagcacg aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg
     3601 gtggtacctc acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag
     3661 aacctagaac ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc
     3721 aaagtagacg gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg
     3781 ggtggaccat cctctagact gccggatcta gctagttaat taaggatcta tggctggaca
     3841 cctggcttca gacttcgcct tctcaccccc accaggtggg ggtgatgggt cagcagggct
     3901 ggagccgggc tgggtggatc ctcgaacctg gctaagcttc caagggcctc caggtgggcc
     3961 tggaatcgga ccaggctcag aggtattggg gatctcccca tgtccgcccg catacgagtt
     4021 ctgcggaggg atggcatact gtggacctca ggttggactg ggcctagtcc cccaagttgg
     4081 cgtggagact ttgcagcctg agggccaggc aggagcacga gtggaaagca actcagaggg
     4141 aacctcctct gagccctgtg ccgaccgccc caatgccgtg aagttggaga aggtggaacc
     4201 aactcccgag gagtcccagg acatgaaagc cctgcagaag gagctagaac agttcgctaa
     4261 gctgctgaag cagaagagga tcaggttggg gtacacccag gccgacgtgg ggctcaccct
     4321 gggcgttctc tttggaaagg tgttcagcca gaccaccatc tgtcgcttcg aggccttgca
     4381 gctcagcctt aagaacatgt gtaagctgcg gcccctgctg gagaagtggg tggaggaagc
     4441 cgacaacaat ccgaaccttc aggagatatg caaatcggag accctggtgc aggcccggaa
     4501 gagaaagcga acgtccatcg aggtgagcgt caagggggct ctggagagcc atttcctcaa
     4561 atgccctaag ccctcggccc aggagatcac ctccctcgcg gacagcttac agctggagaa
     4621 ggaggtggtg cgagtatggt tctgtaacag gcgccagaag ggcaaaagat caagtattga
     4681 gtattcccaa cgagaagagt atgaggctac agggacacct ttcccagggg gggctgtatc
     4741 ctttcctctg cccccaggtc cccactttgg caccccaggc tatggaagcc cccacttcac
     4801 cacactctac tcagtccctt ttcctgaggg cgaggccttt ccctctgttc ccgtcactgc
     4861 tctgggctct cccatgcatt caaactgagc ggccgccagc acagtggtcg acgataaaat
     4921 aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg
     4981 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aaatacataa ctgagaatag
     5041 agaagttcag atcaaggtca ggaacagatg gaacagctga atatgggcca aacaggatat
     5101 ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggaaca gctgaatatg
     5161 ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg
     5221 gtccccagat gcggtccagc cctcagcagt ttctagagaa ccatcagatg tttccagggt
     5281 gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag ttcgcttctc
     5341 gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa cccctcactc
     5401 ggggcgccag tcctccgatt gactgagtcg cccgggtacc cgtgtatcca ataaaccctc
     5461 ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct ctgagtgatt
     5521 gactacccgt cagcgggggt ctttcacatg cagcatgtat caaaattaat ttggtttttt
     5581 ttcttaagta tttacattaa atggccatag ttgcattaat gaatcggcca acgcgcgggg
     5641 agaggcggtt tgcgtattgg gcgct
//
