LOCUS       sequence-410021-        2982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor Vector containing ETV3 transcription factor, part of the Human
            TFome Collection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ng AHM, Khoshakhlagh P, Rojo Arias JE, Pasquini G, Wang K, Swiersy 
            A, Shipman SL, Appleton E, Kiaee K, Kohman RE, Vernet A, Dysart M, 
            Leeper K, Saylor W, Huang JY, Graveline A, Taipale J, Hill DE, Vidal
            M, Melero-Martin JM, Busskamp V, Church GM
  TITLE     A comprehensive library of human transcription factors for cell fate
            engineering.
  JOURNAL   Nat Biotechnol. 2021 Apr;39(4):510-519. doi: 
            10.1038/s41587-020-0742-6. Epub 2020 Nov 30.
  PUBMED    33257861
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-020-0742-6"; journalName: "Nat Biotechnol"; date: 
            "2021-04"; volume: "39"; issue: "4"; pages: "510-519"
COMMENT     Sequence Label: pDONR221-ETV3
FEATURES             Location/Qualifiers
     source          1..2982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(539..638)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(655..674)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(656..674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(679..695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(679..695)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(797..816)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             808..1617
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(875..894)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1764..2352
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2253..2272
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2506..2523
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2682..2709)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2801..2887)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2936..2958
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2950..2967
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2951..2967
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatgaa agccggctgt
      121 agcatcgtgg aaaagccaga aggaggtgga gggtatcagt ttcctgactg ggcctacaaa
      181 acagagtcat ccccaggctc ccggcagatc cagctgtggc acttcatcct ggagctgctg
      241 cagaaggaag agttccgcca tgtcatcgcc tggcagcagg gagagtacgg ggaatttgtc
      301 atcaaggatc cagatgaggt ggcccgcctc tggggccgca ggaaatgcaa accacagatg
      361 aattatgaca agctgagccg ggccctcaga tactattaca acaagaggat ccttcataaa
      421 acaaaaggga aaagatttac ctataaattt aacttcaaca agctggtgat gcccaactac
      481 ccattcatca acattcggtc aagtggtaag atacaaactc ttttggtagg gaatttggac
      541 ccagctttct tgtacaaagt tggcattata agaaagcatt gcttatcaat ttgttgcaac
      601 gaacaggtca ctatcagtca aaataaaatc attatttgcc atccagctga tatcccctat
      661 agtgagtcgt attacatggt catagctgtt tcctggcagc tctggcccgt gtctcaaaat
      721 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
      781 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcgaggccg
      841 cgattaaatt ccaacatgga tgctgattta tatgggtata aatgggctcg cgataatgtc
      901 gggcaatcag gtgcgacaat ctatcgcttg tatgggaagc ccgatgcgcc agagttgttt
      961 ctgaaacatg gcaaaggtag cgttgccaat gatgttacag atgagatggt cagactaaac
     1021 tggctgacgg aatttatgcc tcttccgacc atcaagcatt ttatccgtac tcctgatgat
     1081 gcatggttac tcaccactgc gatccccgga aaaacagcat tccaggtatt agaagaatat
     1141 cctgattcag gtgaaaatat tgttgatgcg ctggcagtgt tcctgcgccg gttgcattcg
     1201 attcctgttt gtaattgtcc ttttaacagc gatcgcgtat ttcgtctcgc tcaggcgcaa
     1261 tcacgaatga ataacggttt ggttgatgcg agtgattttg atgacgagcg taatggctgg
     1321 cctgttgaac aagtctggaa agaaatgcat aaacttttgc cattctcacc ggattcagtc
     1381 gtcactcatg gtgatttctc acttgataac cttatttttg acgaggggaa attaataggt
     1441 tgtattgatg ttggacgagt cggaatcgca gaccgatacc aggatcttgc catcctatgg
     1501 aactgcctcg gtgagttttc tccttcatta cagaaacggc tttttcaaaa atatggtatt
     1561 gataatcctg atatgaataa attgcagttt catttgatgc tcgatgagtt tttctaatca
     1621 gaattggtta attggttgta acactggcag agcattacgc tgacttgacg ggacggcgca
     1681 agctcatgac caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc
     1741 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1801 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1861 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1921 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1981 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2041 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2101 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2161 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2221 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2281 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     2341 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     2401 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     2461 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     2521 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2581 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2641 aatacgcgta ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga
     2701 tggccttctg cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc
     2761 cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag
     2821 cgttcaccga caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt
     2881 ttatttgatg cctggcagtt ccctactctc gcgttaacgc tagcatggat gttttcccag
     2941 tcacgacgtt gtaaaacgac ggccagtctt aagctcgggc cc
//
