LOCUS       sequence-410023-       11622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3xflag fused mouse SOX17FNV in mammalian cells, for 
            lentivirus generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Hu H, Ho DHH, Tan DS, MacCarthy CM, Yu CH, Weng M, Scholer HR, Jauch
            R
  TITLE     Evaluation of the determinants for improved pluripotency induction 
            and maintenance by engineered SOX17.
  JOURNAL   Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597.
  PUBMED    37607832
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597."
COMMENT     Sequence Label: pLVTHM-mSox17FNV-3xflag
FEATURES             Location/Qualifiers
     source          1..11622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1300..1533
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1718..1762
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2147..3325
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2378..3316
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3273..3293
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3373..3490
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             4795..4860
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    4878..5466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4931..4951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5349..5360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    join(5634..5652,5653..5675,5676..5694,5695..5717,5718..5736,
                     5737..5759,5760..5778,5779..5801,5802..5820,5821..5843,
                     5844..5862,5863..5885,5886..5904)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        5933..6147
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     6068..6087
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     protein_bind    complement(6185..6218)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6238..6418
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6443..6461)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6444..6461)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(6751..6770)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(6984..7003)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7103..7125
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7163..7181)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7249..7353
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7354..7422,7423..8214)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7572..7591)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8385..8973
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8874..8893
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9127..9144
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9214..9234)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9219..9548
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9399..9534
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          10682..10747
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             10877..10897
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11322..11456
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11359..11378)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11413..11432
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1201 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1261 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1321 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1381 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1441 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1501 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1561 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1621 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1681 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1741 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1801 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1861 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1921 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1981 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     2041 accatcgcga tgtcgacgat aagctttgca aagatggata aagttttaaa cagagaggaa
     2101 tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct ccggtgcccg
     2161 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2221 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2281 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2341 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2401 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2461 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     2521 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     2581 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     2641 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     2701 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     2761 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     2821 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     2881 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     2941 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3001 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3061 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3121 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3181 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3241 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3301 ttttcttcca tttcaggtgt cgtgaggaat ttcgacattt aaatttaatt aatctcgacg
     3361 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     3421 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     3481 tcaaaatttt atcgatcacg agactagcct cgaggtttaa acatgagcag cccggatgcg
     3541 ggatacgcca gtgacgacca gagccagccc cggagcgcgc agcccgcggt gatggcaggg
     3601 ttgggcccct gtccctgggc cgagtccctg agccccctcg gggatgtaaa ggtgaaaggc
     3661 gaggtggtgg cgagtagcgg ggcgccagcc gggacgtcgg gccgagccaa agcggagtct
     3721 cgcatccggc ggccgatgaa cgcctttatg gtgtgggcca aagacgaacg caagcggttg
     3781 gcacagcaga acccagatct gcacaacgca gagctaagca agatgctagg caagtcttgg
     3841 aaggcgttga cctttgcaga gaagcggccc ttcaatgaag aggccgtgcg gctgcgcgtg
     3901 cagcatatgc aggaccaccc caactacaag taccggccgc ggcggcgcaa gcaggtgaag
     3961 cgcatgaagc gggtggaggg aggcttcctg cacgctctcg tcgagcccca ggccggcgcg
     4021 cttggtcccg agggcggccg cgtggccatg gatggcctgg gtctgccttt cccggagccg
     4081 ggctatccgg ccggtcctcc gctgatgtct ccgcacatgg gcccccacta tcgggactgc
     4141 cagggactgg gcgctcccgc gctcgacggc taccctctgc ccactccgga cacatccccg
     4201 ctggatggcg tggagcagga cccggctttc tttgcagccc cgctgccagg ggactgcccg
     4261 gcggccggca cctacactta cgctccagtc tcggactatg cagtgtccgt agagccgccc
     4321 gctggcccca tgcgagtggg gccggacccc tcgggccctg cgatgccggg gatcctggcg
     4381 ccccccagcg ctctgcacct gtactacggc gcgatgggct cgcccgccgc aagtgcgggg
     4441 cgcggtttcc acgcgcaacc ccagcagccg ctgcaaccgc aggcaccgcc cccgccaccg
     4501 cagcagcagc acccagcgca cggccccggg caaccttcgc cccctcccga ggctctgccc
     4561 tgccgggatg gcacggaatc caaccagccc actgagctcc taggggaggt ggaccgcacg
     4621 gaattcgaac agtatctgcc ctttgtgtat aagcccgaga tgggtcttcc ctaccaggga
     4681 cacgactgcg gagtgaacct ctcagacagc cacggagcca tttcctccgt ggtgtccgac
     4741 gctagctcag cggtctacta ttgcaactac cccgacatta cgcgtggcag cggcgactac
     4801 aaggaccacg acggcgacta caaggaccac gacatcgact acaaggacga cgacgacaag
     4861 tagactagtc atatgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     4921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     4981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     5041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     5101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     5161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     5221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     5281 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     5341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     5401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     5461 ccccgcatcg gtacgtatgg ccaggtacct ttaagaccaa tgacttacaa ggcagctgta
     5521 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5581 agacaagatg ggatcaattc accatgctag tggatccccc tcgagtttac cactccctat
     5641 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     5701 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     5761 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     5821 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5881 accactccct atcagtgata gagaaaagtg aaagtcgggg ctgcaggaat tcgaacgctg
     5941 acgtcatcaa cccgctccaa ggaatcgcgg gcccagtgtc actaggcggg aacacccagc
     6001 gcgcgtgcgc cctggcagga agatggctgt gagggacagg ggagtggcgc cctgcaatat
     6061 ttgcatgtcg ctatgtgttc tgggaaatca ccataaacgt gaaatgtctt tggatttggg
     6121 aatcttataa gttctgtatg agaccacgcg gcgtctccaa gcttatcgat accgtcgcat
     6181 gggaataact tcgtatagca tacattatac gaagttatgc tgctttttgc ttgtactggg
     6241 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     6301 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     6361 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc
     6421 atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata
     6481 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag
     6541 catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt
     6601 tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg
     6661 aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca
     6721 tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag
     6781 atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc
     6841 ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg
     6901 tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag
     6961 agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
     7021 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     7081 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     7141 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     7201 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     7261 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7321 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7381 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7441 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7501 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7561 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7621 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7681 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7741 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7801 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7861 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7921 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7981 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8041 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8101 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8161 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8221 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8281 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8341 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8401 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8461 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8521 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8581 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8641 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8701 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8761 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8821 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8881 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8941 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9001 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9061 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9121 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9181 cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa
     9241 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     9301 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     9361 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     9421 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     9481 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     9541 tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat
     9601 cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt
     9661 agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc
     9721 gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg
     9781 ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc
     9841 attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta
     9901 ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta
     9961 aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg
    10021 ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca
    10081 tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag
    10141 atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc
    10201 gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta
    10261 caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa
    10321 accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat
    10381 cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat
    10441 cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac
    10501 gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg
    10561 gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga
    10621 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa
    10681 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt
    10741 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga
    10801 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc
    10861 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc
    10921 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc
    10981 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc
    11041 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc
    11101 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt
    11161 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca
    11221 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
    11281 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
    11341 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    11401 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    11461 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    11521 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    11581 aaagaaattg tatttgttaa atatgtacta caaacttagt ag
//
