LOCUS       sequence-410029-       13689 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses four genes (human OCT4, KLF4, c-MYC, SOX17FNV) in 
            mammalian cells, for lentivirus generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Hu H, Ho DHH, Tan DS, MacCarthy CM, Yu CH, Weng M, Scholer HR, Jauch
            R
  TITLE     Evaluation of the determinants for improved pluripotency induction 
            and maintenance by engineered SOX17.
  JOURNAL   Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597.
  PUBMED    37607832
REFERENCE   2  (bases 1 to 13689)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597."
COMMENT     Sequence Label: FUW-tetO-hOKMSOX17FNV
FEATURES             Location/Qualifiers
     source          1..13689
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3957..4010
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5430..5486
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5487..6803
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             6813..6872
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             7821..7838
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             7827..7844
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     misc_feature    8150..8738
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8203..8223)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8621..8632)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8741..8757)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8742..8758)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9263..9443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9469..9486)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9475..9699
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9745..10173
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9832..9851)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10042..10063
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10182..10202)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10187..10516
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10367..10502
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10429..10448
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10564..10611
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10630..11004
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11134..11255
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11171..11190)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11225..11244
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11304..11320)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11304..11320)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11317..11339)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11328..11344
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11352..11358,11359..11376,11377..11382))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11397..11418
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11535..11552)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11706..12294)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11786..11805)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12465..13256,13257..13325))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13088..13107
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13326..13430)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13505..13524)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcgttgacc gaatcaccga cctctctccc cagggggatc caccggtatg gcgggacacc
     2881 tggcttcgga tttcgccttc tcgccccctc caggtggtgg aggtgatggg ccaggggggc
     2941 cggagccggg ctgggttgat cctcggacct ggctaagctt ccaaggccct cctggagggc
     3001 caggaatcgg gccgggggtt gggccaggct ctgaggtgtg ggggattccc ccatgccccc
     3061 cgccgtatga gttctgtggg gggatggcgt actgtgggcc ccaggttgga gtggggctag
     3121 tgccccaagg cggcttggag acctctcagc ctgagggtga agcaggagtc ggggtggaga
     3181 gcaactccga tggggcctcc ccggagccct gcaccgtcac ccctggtgcc gtgaagctgg
     3241 agaaggagaa gctggagcaa aacccggagg agtcccagga catcaaagct ctgcagaaag
     3301 aactcgagca atttgccaag ctcctgaagc agaagaggat caccctggga tatacacagg
     3361 ccgatgtggg gctcaccctg ggggttctat ttgggaaggt attcagccaa acgaccatct
     3421 gccgctttga ggctctgcag cttagcttca agaacatgtg taagctgcgg cccttgctgc
     3481 agaagtgggt ggaggaagct gacaacaatg aaaatcttca ggagatatgc aaagcagaaa
     3541 ccctcgtgca ggcccgaaag agaaagcgaa ccagtatcga gaaccgagtg agaggcaacc
     3601 tggagaattt gttcctgcag tgcccgaaac ccacactgca gcagatcagc cacatcgccc
     3661 agcagcttgg gctcgagaag gatgtggtcc gagtgtggtt ctgtaaccgg cgccagaagg
     3721 gcaagcgatc aagcagcgac tatgcacaac gagaggattt tgaggctgct gggtctcctt
     3781 tctcaggggg accagtgtcc tttcctctgg ccccagggcc ccattttggt accccaggct
     3841 atgggagccc tcacttcact gcactgtact cctcggtccc tttccctgag ggggaagcct
     3901 ttccccctgt ctctgtcacc actctgggct ctcccatgca ttcaaacgga tctggagagg
     3961 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggcccc atggctgtca
     4021 gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga agggagaaga
     4081 cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc cacatgaagc
     4141 gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc gtggccacag
     4201 acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg cccctacctc
     4261 ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc tccaattcgc
     4321 tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca gcctcctctt
     4381 cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc ttcacctatc
     4441 cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga ggcctcctct
     4501 atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac atcaacgacg
     4561 tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac ccggtgtaca
     4621 ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc gtgctgaagg
     4681 cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc gtcagcaaag
     4741 gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg ccgccgcgca
     4801 cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc gctggacccc
     4861 ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg cagctcccca
     4921 gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac tgtcaccctg
     4981 ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca tccttcctgc
     5041 ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg ccacccggtt
     5101 cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc cggaaaagga
     5161 ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag agttcccatc
     5221 tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac tgggacggct
     5281 gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa cacacggggc
     5341 accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac cacctcgcct
     5401 tacacatgaa gaggcatttt ggatctggag caacaaactt ctcactactc aaacaagcag
     5461 gtgacgtgga ggagaatccc ggccctatgc ccctcaacgt tagcttcacc aacaggaact
     5521 atgacctcga ctacgactcg gtgcagccgt atttctactg cgacgaggag gagaacttct
     5581 accagcagca gcagcagagc gagctgcagc ccccggcgcc cagcgaggat atctggaaga
     5641 aattcgagct gctgcccacc ccgcccctgt cccctagccg ccgctccggg ctctgctcgc
     5701 cctcctacgt tgcggtcaca cccttctccc ttcggggaga caacgacggc ggtggcggga
     5761 gcttctccac ggccgaccag ctggagatgg tgaccgagct gctgggagga gacatggtga
     5821 accagagttt catctgcgac ccggacgacg agaccttcat caaaaacatc atcatccagg
     5881 actgtatgtg gagcggcttc tcggccgccg ccaagctcgt ctcagagaag ctggcctcct
     5941 accaggctgc gcgcaaagac agcggcagcc cgaaccccgc ccgcggccac agcgtctgct
     6001 ccacctccag cttgtacctg caggatctga gcgccgccgc ctcagagtgc atcgacccct
     6061 cggtggtctt cccctaccct ctcaacgaca gcagctcgcc caagtcctgc gcctcgcaag
     6121 actccagcgc cttctctccg tcctcggatt ctctgctctc ctcgacggag tcctccccgc
     6181 agggcagccc cgagcccctg gtgctccatg aggagacacc gcccaccacc agcagcgact
     6241 ctgaggagga acaagaagat gaggaagaaa tcgatgttgt ttctgtggaa aagaggcagg
     6301 ctcctggcaa aaggtcagag tctggatcac cttctgctgg aggccacagc aaacctcctc
     6361 acagcccact ggtcctcaag aggtgccacg tctccacaca tcagcacaac tacgcagcgc
     6421 ctccctccac tcggaaggac tatcctgctg ccaagagggt caagttggac agtgtcagag
     6481 tcctgagaca gatcagcaac aaccgaaaat gcaccagccc caggtcctcg gacaccgagg
     6541 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
     6601 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
     6661 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
     6721 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
     6781 aacagctacg gaactcttgt gcgggatctg gacaatgtac taactacgct ttgttgaaac
     6841 tcgctggcga tgttgaaagt aaccccggtc ccatgagcag cccggatgcg ggatacgcca
     6901 gtgacgacca gagccagacc cagagcgcgc tgcccgcggt gatggccggg ctgggcccct
     6961 gcccctgggc cgagtcgctg agccccatcg gggacatgaa ggtgaagggc gaggcgccgg
     7021 cgaacagcgg agcaccggcc ggggccgcgg gccgagccaa gggcgagtcc cgtatccggc
     7081 ggccgatgaa cgctttcatg gtgtgggcta aggacgagcg caagcggctg gcgcagcaga
     7141 atccagacct gcacaacgcc gagttgagca agatgctggg caagtcgtgg aaggcgctga
     7201 cgtttgcgga gaagcggccc ttcaatgagg aggcagtgcg gctgcgcgtg cagcacatgc
     7261 aggaccaccc caactacaag taccggccgc ggcggcgcaa gcaggtgaag cggctgaagc
     7321 gggtggaggg cggcttcctg cacggcctgg ctgagccgca ggcggccgcg ctgggccccg
     7381 agggcggccg cgtggccatg gacggcctgg gcctccagtt ccccgagcag ggcttccccg
     7441 ccggcccgcc gctgctgcct ccgcacatgg gcggccacta ccgcgactgc cagagtctgg
     7501 gcgcgcctcc gctcgacggc tacccgttgc ccacgcccga cacgtccccg ctggacggcg
     7561 tggaccccga cccggctttc ttcgccgccc cgatgcccgg ggactgcccg gcggccggca
     7621 cctacagcta cgcgcaggtc tcggactacg ctggcccccc ggagcctccc gccggtccca
     7681 tgcacccccg actcggccca gagcccgcgg gtccctcgat tccgggcctc ctggcgccac
     7741 ccagcgccct tcacgtgtac tacggcgcga tgggctcgcc cggggcgggc ggcgggcgcg
     7801 gcttccagat gcagccgcaa caccagcacc agcaccagca ccagcaccac cccccgggcc
     7861 ccggacagcc gtcgccccct ccggaggcac tgccctgccg ggacggcacg gaccccagtc
     7921 agcccgccga gctcctcggg gaggtggacc gcacggaatt tgaacagtat ctgcacttcg
     7981 tgtgcaagcc tgagatgggc ctcccctacc aggggcatga ctccggtgtg aatctccccg
     8041 acagccacgg ggccatttcc tcggtggtgt ccgacgccag ctccgcggta tattactgca
     8101 actatcctga cgtgtgagtc gacaagaatt cgatatcaag cttatcgata atcaacctct
     8161 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     8221 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     8281 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     8341 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     8401 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     8461 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     8521 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     8581 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     8641 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     8701 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgaga
     8761 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     8821 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     8881 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     8941 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     9001 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     9061 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     9121 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     9181 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     9241 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     9301 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     9361 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     9421 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     9481 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     9541 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     9601 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     9661 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     9721 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     9781 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     9841 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     9901 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     9961 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
    10021 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
    10081 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
    10141 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
    10201 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
    10261 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
    10321 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
    10381 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
    10441 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
    10501 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
    10561 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
    10621 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
    10681 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
    10741 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
    10801 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
    10861 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
    10921 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
    10981 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
    11041 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
    11101 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
    11161 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
    11221 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
    11281 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
    11341 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
    11401 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    11461 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    11521 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
    11581 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
    11641 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
    11701 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
    11761 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
    11821 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
    11881 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
    11941 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
    12001 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
    12061 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
    12121 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
    12181 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
    12241 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    12301 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    12361 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    12421 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    12481 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    12541 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    12601 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    12661 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    12721 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    12781 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    12841 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    12901 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    12961 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    13021 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    13081 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    13141 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    13201 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    13261 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    13321 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    13381 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    13441 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    13501 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    13561 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    13621 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    13681 ccagatata
//
