LOCUS       sequence-410030-       13326 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in 
            mammalian cells, for lentivirus generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13326)
  AUTHORS   Hu H, Ho DHH, Tan DS, MacCarthy CM, Yu CH, Weng M, Scholer HR, Jauch
            R
  TITLE     Evaluation of the determinants for improved pluripotency induction 
            and maintenance by engineered SOX17.
  JOURNAL   Nucleic Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597.
  PUBMED    37607832
REFERENCE   2  (bases 1 to 13326)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2023 Aug 23:gkad597. doi: 10.1093/nar/gkad597."
COMMENT     Sequence Label: FUW-tetO-hOKMminiSOX
FEATURES             Location/Qualifiers
     source          1..13326
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        7..386
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        388..586
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             604..784
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    831..956
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1449..1682
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1867..1911
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2209..2326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2383..2401,2402..2424,2425..2443,2444..2466,2467..2485,
                     2486..2508,2509..2527,2528..2550,2551..2569,2570..2592,
                     2593..2611,2612..2634,2635..2653)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2686..2724
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2721..2745
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3957..4010
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5430..5486
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5487..6803
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             6813..6872
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     misc_feature    7787..8375
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7840..7860)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8258..8269)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8378..8394)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8379..8395)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8900..9080
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9106..9123)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9112..9336
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9382..9810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9469..9488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9679..9700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9819..9839)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9824..10153
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10004..10139
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10066..10085
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10201..10248
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             10267..10641
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10771..10892
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10808..10827)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10862..10881
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10941..10957)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10941..10957)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10954..10976)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10965..10981
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10989..10995,10996..11013,11014..11019))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11034..11055
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11172..11189)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11343..11931)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11423..11442)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12102..12893,12894..12962))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12725..12744
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12963..13067)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(13142..13161)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
       61 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
      121 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
      181 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
      241 tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc
      301 ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct
      361 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg
      421 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
      481 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga
      541 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt
      601 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
      661 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
      721 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
      781 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
      841 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
      901 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
      961 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     1021 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     1081 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     1141 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     1201 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     1261 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga
     1321 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     1381 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     1441 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     1501 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     1561 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     1621 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     1681 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     1741 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     1801 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     1861 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     1921 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     1981 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     2041 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     2101 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg
     2161 gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa
     2221 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     2281 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     2341 ggacagcaga gatccagttt ggttagatct cgagtttacc actccctatc agtgatagag
     2401 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     2461 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     2521 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2581 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2641 tcagtgatag agaaaagtga aagtcgagct cggtacccgg gtcgaggtag gcgtgtacgg
     2701 tgggaggcct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
     2761 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg cggccccgaa
     2821 ttcgttgacc gaatcaccga cctctctccc cagggggatc caccggtatg gcgggacacc
     2881 tggcttcgga tttcgccttc tcgccccctc caggtggtgg aggtgatggg ccaggggggc
     2941 cggagccggg ctgggttgat cctcggacct ggctaagctt ccaaggccct cctggagggc
     3001 caggaatcgg gccgggggtt gggccaggct ctgaggtgtg ggggattccc ccatgccccc
     3061 cgccgtatga gttctgtggg gggatggcgt actgtgggcc ccaggttgga gtggggctag
     3121 tgccccaagg cggcttggag acctctcagc ctgagggtga agcaggagtc ggggtggaga
     3181 gcaactccga tggggcctcc ccggagccct gcaccgtcac ccctggtgcc gtgaagctgg
     3241 agaaggagaa gctggagcaa aacccggagg agtcccagga catcaaagct ctgcagaaag
     3301 aactcgagca atttgccaag ctcctgaagc agaagaggat caccctggga tatacacagg
     3361 ccgatgtggg gctcaccctg ggggttctat ttgggaaggt attcagccaa acgaccatct
     3421 gccgctttga ggctctgcag cttagcttca agaacatgtg taagctgcgg cccttgctgc
     3481 agaagtgggt ggaggaagct gacaacaatg aaaatcttca ggagatatgc aaagcagaaa
     3541 ccctcgtgca ggcccgaaag agaaagcgaa ccagtatcga gaaccgagtg agaggcaacc
     3601 tggagaattt gttcctgcag tgcccgaaac ccacactgca gcagatcagc cacatcgccc
     3661 agcagcttgg gctcgagaag gatgtggtcc gagtgtggtt ctgtaaccgg cgccagaagg
     3721 gcaagcgatc aagcagcgac tatgcacaac gagaggattt tgaggctgct gggtctcctt
     3781 tctcaggggg accagtgtcc tttcctctgg ccccagggcc ccattttggt accccaggct
     3841 atgggagccc tcacttcact gcactgtact cctcggtccc tttccctgag ggggaagcct
     3901 ttccccctgt ctctgtcacc actctgggct ctcccatgca ttcaaacgga tctggagagg
     3961 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggcccc atggctgtca
     4021 gcgacgcgct gctcccatct ttctccacgt tcgcgtctgg cccggcggga agggagaaga
     4081 cactgcgtca agcaggtgcc ccgaataacc gctggcggga ggagctctcc cacatgaagc
     4141 gacttccccc agtgcttccc ggccgcccct atgacctggc ggcggcgacc gtggccacag
     4201 acctggagag cggcggagcc ggtgcggctt gcggcggtag caacctggcg cccctacctc
     4261 ggagagagac cgaggagttc aacgatctcc tggacctgga ctttattctc tccaattcgc
     4321 tgacccatcc tccggagtca gtggccgcca ccgtgtcctc gtcagcgtca gcctcctctt
     4381 cgtcgtcgcc gtcgagcagc ggccctgcca gcgcgccctc cacctgcagc ttcacctatc
     4441 cgatccgggc cgggaacgac ccgggcgtgg cgccgggcgg cacgggcgga ggcctcctct
     4501 atggcaggga gtccgctccc cctccgacgg ctcccttcaa cctggcggac atcaacgacg
     4561 tgagcccctc gggcggcttc gtggccgagc tcctgcggcc agaattggac ccggtgtaca
     4621 ttccgccgca gcagccgcag ccgccaggtg gcgggctgat gggcaagttc gtgctgaagg
     4681 cgtcgctgag cgcccctggc agcgagtacg gcagcccgtc ggtcatcagc gtcagcaaag
     4741 gcagccctga cggcagccac ccggtggtgg tggcgcccta caacggcggg ccgccgcgca
     4801 cgtgccccaa gatcaagcag gaggcggtct cttcgtgcac ccacttgggc gctggacccc
     4861 ctctcagcaa tggccaccgg ccggctgcac acgacttccc cctggggcgg cagctcccca
     4921 gcaggactac cccgaccctg ggtcttgagg aagtgctgag cagcagggac tgtcaccctg
     4981 ccctgccgct tcctcccggc ttccatcccc acccggggcc caattaccca tccttcctgc
     5041 ccgatcagat gcagccgcaa gtcccgccgc tccattacca agagctcatg ccacccggtt
     5101 cctgcatgcc agaggagccc aagccaaaga ggggaagacg atcgtggccc cggaaaagga
     5161 ccgccaccca cacttgtgat tacgcgggct gcggcaaaac ctacacaaag agttcccatc
     5221 tcaaggcaca cctgcgaacc cacacaggtg agaaacctta ccactgtgac tgggacggct
     5281 gtggatggaa attcgcccgc tcagatgaac tgaccaggca ctaccgtaaa cacacggggc
     5341 accgcccgtt ccagtgccaa aaatgcgacc gagcattttc caggtcggac cacctcgcct
     5401 tacacatgaa gaggcatttt ggatctggag caacaaactt ctcactactc aaacaagcag
     5461 gtgacgtgga ggagaatccc ggccctatgc ccctcaacgt tagcttcacc aacaggaact
     5521 atgacctcga ctacgactcg gtgcagccgt atttctactg cgacgaggag gagaacttct
     5581 accagcagca gcagcagagc gagctgcagc ccccggcgcc cagcgaggat atctggaaga
     5641 aattcgagct gctgcccacc ccgcccctgt cccctagccg ccgctccggg ctctgctcgc
     5701 cctcctacgt tgcggtcaca cccttctccc ttcggggaga caacgacggc ggtggcggga
     5761 gcttctccac ggccgaccag ctggagatgg tgaccgagct gctgggagga gacatggtga
     5821 accagagttt catctgcgac ccggacgacg agaccttcat caaaaacatc atcatccagg
     5881 actgtatgtg gagcggcttc tcggccgccg ccaagctcgt ctcagagaag ctggcctcct
     5941 accaggctgc gcgcaaagac agcggcagcc cgaaccccgc ccgcggccac agcgtctgct
     6001 ccacctccag cttgtacctg caggatctga gcgccgccgc ctcagagtgc atcgacccct
     6061 cggtggtctt cccctaccct ctcaacgaca gcagctcgcc caagtcctgc gcctcgcaag
     6121 actccagcgc cttctctccg tcctcggatt ctctgctctc ctcgacggag tcctccccgc
     6181 agggcagccc cgagcccctg gtgctccatg aggagacacc gcccaccacc agcagcgact
     6241 ctgaggagga acaagaagat gaggaagaaa tcgatgttgt ttctgtggaa aagaggcagg
     6301 ctcctggcaa aaggtcagag tctggatcac cttctgctgg aggccacagc aaacctcctc
     6361 acagcccact ggtcctcaag aggtgccacg tctccacaca tcagcacaac tacgcagcgc
     6421 ctccctccac tcggaaggac tatcctgctg ccaagagggt caagttggac agtgtcagag
     6481 tcctgagaca gatcagcaac aaccgaaaat gcaccagccc caggtcctcg gacaccgagg
     6541 agaatgtcaa gaggcgaaca cacaacgtct tggagcgcca gaggaggaac gagctaaaac
     6601 ggagcttttt tgccctgcgt gaccagatcc cggagttgga aaacaatgaa aaggccccca
     6661 aggtagttat ccttaaaaaa gccacagcat acatcctgtc cgtccaagca gaggagcaaa
     6721 agctcatttc tgaagaggac ttgttgcgga aacgacgaga acagttgaaa cacaaacttg
     6781 aacagctacg gaactcttgt gcgggatctg gacaatgtac taactacgct ttgttgaaac
     6841 tcgctggcga tgttgaaagt aaccccggtc ccatgtctcg catccggcgg ccgatgaacg
     6901 cctttatggt gtgggccaaa gacgaacgca agcggttggc acagcagaac ccagatctgc
     6961 acaacgcaga gctaagcaag atgctaggca agtcttggaa ggcgttgacc tttgcagaga
     7021 agcggccctt caatgaagag gccgtgcggc tgcgcgtgca gcatatgcag gaccacccca
     7081 actacaagta ccggccgcgg cggcgcaagc aggtgaagcg catgaagcgg gtggagggag
     7141 gcttcctgca cgctctcgtc gagccccagg ccggcgcgct tggtcccgag ggcggccgcg
     7201 tggccatgga tggcctgggt ctgcctttcc cggagccggg ctatccggcc ggtcctccgc
     7261 tgatgtctcc gcacatgggc ccccactatc gggactgcca gggactgggc gctcccgcgc
     7321 tcgacggcta ccctctgccc actccggaca catccccgct ggatggcgtg gagcaggacc
     7381 cggctttctt tgcagccccg ctgccagggg actgcccggc ggccggcacc tacacttacg
     7441 ctccagtctc ggactatgca gtgtccgtag agccgcccgc tggccccatg cgagtggggc
     7501 cggacccctc gggccctgcg atgccgggga tcctggcgcc ccccagcgct ctgcacctgt
     7561 actacggcgc gatgggctcg cccgccgcaa gtgcggggcg cggtttccac gcgcaacccc
     7621 agcagccgct gcaaccgcag gcagagctcc taggggaggt ggaccgcacg gaattcgaac
     7681 agtatctgcc ctttgtgtat aagcccgaga tgggtcttcc ctaccaggga cacgactgcg
     7741 gagtgaacct ctaagtcgac aagaattcga tatcaagctt atcgataatc aacctctgga
     7801 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7861 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7921 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7981 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     8041 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     8101 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     8161 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     8221 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     8281 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     8341 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagacct
     8401 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     8461 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
     8521 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     8581 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
     8641 acaaggctac ttccctgatt ggcagaacta cacaccaggg ccagggatca gatatccact
     8701 gacctttgga tggtgctaca agctagtacc agttgagcaa gagaaggtag aagaagccaa
     8761 tgaaggagag aacacccgct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8821 gagagaagta ttagagtgga ggtttgacag ccgcctagca tttcatcaca tggcccgaga
     8881 gctgcatccg gactgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     8941 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     9001 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     9061 agtgtggaaa atctctagca gggcccgttt aaacccgctg atcagcctcg actgtgcctt
     9121 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     9181 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     9241 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     9301 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     9361 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     9421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     9481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     9541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     9601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     9661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     9721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     9781 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
     9841 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9901 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9961 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    10021 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10081 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10141 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
    10201 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
    10261 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
    10321 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
    10381 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
    10441 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
    10501 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
    10561 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
    10621 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
    10681 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
    10741 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
    10801 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
    10861 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
    10921 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10981 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    11041 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    11101 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
    11161 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
    11221 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
    11281 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
    11341 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    11401 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    11461 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    11521 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    11581 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    11641 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    11701 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    11761 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    11821 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    11881 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    11941 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    12001 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    12061 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    12121 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    12181 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    12241 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    12301 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    12361 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    12421 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    12481 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    12541 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    12601 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    12661 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    12721 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    12781 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    12841 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    12901 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    12961 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    13021 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    13081 aaagtgccac ctgacgtcga cggatcggga gatctcccga tcccctatgg tgcactctca
    13141 gtacaatctg ctctgatgcc gcatagttaa gccagtatct gctccctgct tgtgtgttgg
    13201 aggtcgctga gtagtgcgcg agcaaaattt aagctacaac aaggcaaggc ttgaccgaca
    13261 attgcatgaa gaatctgctt agggttaggc gttttgcgct gcttcgcgat gtacgggcca
    13321 gatata
//
