LOCUS       sequence-410038-        6896 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDefence14:Eluc (defence-induced promoter with green-shifted 
            luciferase expression cassette).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6896)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pBD001
FEATURES             Location/Qualifiers
     source          1..6896
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             2130..2138
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2299..2551
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2599..2623
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2779..3927)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4263..4851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4343..4362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5022..5813,5814..5882))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5645..5664
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5883..5987)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6074..6705
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6797..6821
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gaggttctct caacttagaa actttacatg
       61 ttttttttag gaataaaata ttctctttac aaaagtcatc ttttttcacc gcgtaagtta
      121 ttgtgggctt ttttttacac aagatttggc atgatcctgc gggtgcgaca ggacgtcagc
      181 agcaacgatg tgtacactac gaccagtgca aacaattacg gtcaaacttc tgatacgcgt
      241 gacgctcaac caaatatagt actagtagta ctaaaataac tgagtcattt tcttaagagc
      301 gaagaaagat cggttttata aatggaaaac attcatagtg gagctaatga gcaaccaatc
      361 aaaaccatcc tcacaaaccg taaagatccc ctgcttccgc gaagtaccct actgccttct
      421 ctccgatcaa atcgtctctc tccatcccgt tgctcgtaag tcgcaacccg cgaaatttca
      481 acaccaaaaa atcaggcgag cctgcccaaa gcaaacgcgt ccagaacggc caggacgcgt
      541 caagaacgcc gtataaatac catcacgagc accatcgttg gtccatcagt cacctatctc
      601 accaacacat ccgtcgcctt ctcgagcaga tcaccagttc agttcaggac gcgcagaatg
      661 gagagagaga agaacgtggt gtacggcccc gagcccaagc accctctggg caacttcacc
      721 gccggcgaga tgctgtacaa cgctctgcac aagcactccc acatccccca ggccatcctg
      781 gacgtgatgg gcaacgagtc cctttcctac caggagttct tcgacactac tgtgaagctg
      841 ggccagagcc tccagaactg tggctacaag atgaacgatg tcgtgtcgat ctgtgcagag
      901 aacaacaaga gattcttcat ccccatcatc tccgcctggt acatcggcat ggtggtggcc
      961 cctgtgaacg aggactatat cccagacgag ctgtgtaaag tgaccggcat ctccaagccg
     1021 atcctggtct ttaccactag gaagatcctg cctaaggttt tggaggttaa agacagaacc
     1081 aactacataa agaggatcat catactggac tctgaagaga acctgctggg ctgcgagagc
     1141 ctgcacaact tcatgtccag gtactccgac aacaacctcc aaacattcaa gcctctgcac
     1201 tacgaccctg tggaccaggt agccgccatc ctgtgctcct ccggcacaac cggcctgcct
     1261 aaaggcgtga tgcagaccca caggaacatc tgtgtgagac tcacacacgc atctgacccc
     1321 agagtgggta cacaactcat ccccggcgta tccgtgctgg cctacctgcc attcttccac
     1381 gccttcggct tcagtatcaa cctgggctat ttcatggtgg gcctgagagt ggtgatgctc
     1441 cgaaggttta accaggaggt gttcctgaag gccatccagg actacgaggt gaggagcgtg
     1501 atcaacgttc cctccacaat cctgttcctg tccaagagcc ctctggtgga caagtacgac
     1561 ctatccaccc tggcggagct gtgctgtgga gccgctcctc tggcgaagga ggtggccgag
     1621 atcgccgtga agaggctgaa cctgccaggg atacggtgtg gctacggtct aacagagtct
     1681 acctccgcca acatccatac tctgcacaac gagttcaagt ccggctccct gggcaaggtg
     1741 acaccttaca tggccgccaa gatcatcgac aggaacaccg gcgaggccct gggtccaaac
     1801 caggtgggcg agctgtgcat ctggggacct atggtaacaa aaggctatgt gaacaaccca
     1861 caggctacta aggaggccat cgacgacgac ggctggctgc actctggcga cttcggctac
     1921 tacgacgagg acgagtattt ctacatcgtg gaccggtaca aggagctgat caaatacaag
     1981 ggctatcagg tcgcccctgt ggagctggag gagatcctcc ttcagcaccc aggcatcagg
     2041 gacgtggccg tcgtgggtat ccctgacatc gaggccggcg agctgccagc cggcttcgtg
     2101 gtgaagcagc ccggcgccca actcaccgct aaggaggtgt acgacttcct ggcccagagg
     2161 gtgtctcact ccaagtacct gaggggcggc gtaaggttcg tggactctat ccccaggaac
     2221 gtgacaggca agattagtcg aaaagagctg agggaggccc tgatggagaa ggcttctaag
     2281 ctgtaagctt gtcaagcaga tcgttcaaac atttggcaat aaagtttctt aagattgaat
     2341 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
     2401 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
     2461 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
     2521 tcgcgcgcgg tgtcatctat gttactagat cgacgctgca attgtcttct gcacgaagtg
     2581 gtttaaacta tcagtgtttg acaggatata ttggcgggta aacctaagag aaaagagcgt
     2641 ttattagaat aatcggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt
     2701 atgtgcatgc caaccacagg gttccccaga tcaggcgctg gctgctgaac ccccagccgg
     2761 aactgacccc acaaggccct agcgtttgca atgcaccagg tcatcattga cccaggcgtg
     2821 ttccaccagg ccgctgcctc gcaactcttc gcaggcttcg ccgacctgct cgcgccactt
     2881 cttcacgcgg gtggaatccg atccgcacat gaggcggaag gtttccagct tgagcgggta
     2941 cggctcccgg tgcgagctga aatagtcgaa catccgtcgg gccgtcggcg acagcttgcg
     3001 gtacttctcc catatgaatt tcgtgtagtg gtcgccagca aacagcacga cgatttcctc
     3061 gtcgatcagg acctggcaac gggacgtttt cttgccacgg tccaggacgc ggaagcggtg
     3121 cagcagcgac accgattcca ggtgcccaac gcggtcggac gtgaagccca tcgccgtcgc
     3181 ctgtaggcgc gacaggcatt cctcggcctt cgtgtaatac cggccattga tcgaccagcc
     3241 caggtcctgg caaagctcgt agaacgtgaa ggtgatcggc tcgccgatag gggtgcgctt
     3301 cgcgtactcc aacacctgct gccacaccag ttcgtcatcg tcggcccgca gctcgacgcc
     3361 ggtgtaggtg atcttcacgt ccttgttgac gtggaaaatg accttgtttt gcagcgcctc
     3421 gcgcgggatt ttcttgttgc gcgtggtgaa cagggcagag cgggccgtgt cgtttggcat
     3481 cgctcgcatc gtgtccggcc acggcgcaat atcgaacaag gaaagctgca tttccttgat
     3541 ctgctgcttc gtgtgtttca gcaacgcggc ctgcttggcc tcgctgacct gttttgccag
     3601 gtcctcgccg gcggtttttc gcttcttggt cgtcatagtt cctcgcgtgt cgatggtcat
     3661 cgacttcgcc aaacctgccg cctcctgttc aagacgacgc gaacgctcca cggcggccga
     3721 tggcgcgggc agggcagggg gagccagttg cacgctgtcg cgctcgatct tggccgtagc
     3781 ttgctggacc atcgagccga cggactggaa ggtttcgcgg ggcgcacgca tgacggtgcg
     3841 gcttgcgatg gtttcggcat cctcggcgga aaaccccgcg tcgatcagtt cttgcctgta
     3901 tgccttccgg tcaaacgtcc gattcattca ccctccttgc gggattgccc cgactcacgc
     3961 cggggcaatg tgcccttatt cctgatttga cccgcctggt gccttggtgt ccagataatc
     4021 caccttatcg gcaatgaagt cggtcccgta gaccgtctgg ccgtccttct cgtacttggt
     4081 attccgaatc ttgccctgca cgaataccag cgaccccttg cccaaatact tgccgtgggc
     4141 ctcggcctga gagccaaaac acttgatgcg gaagaagtcg gtgcgctcct gcttgtcgcc
     4201 ggcatcgttg cgccacatct aggatctgcc aggaaccgta aaaaggccgc gttgctggcg
     4261 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4321 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4381 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4441 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4501 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4561 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4621 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4681 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4741 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4801 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4861 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4921 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4981 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     5041 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     5101 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     5161 cgagaaccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     5221 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     5281 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     5341 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     5401 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     5461 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     5521 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     5581 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     5641 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     5701 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     5761 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     5821 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     5881 atactcttcc tttttcaata ttattgaagc atttatcaag gttattgtct catgagcgga
     5941 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac gaattggcca
     6001 gcgctgccat ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg
     6061 ggaggcccgc gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg
     6121 cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta
     6181 aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg
     6241 ctggattttc tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca
     6301 gcactctgcc cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc
     6361 aagtgtcaat accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc
     6421 gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga
     6481 aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca
     6541 acgtccgccc ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg
     6601 cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag
     6661 acggccgcca gcccagcggc gagggcaacc agcccggtga gcgtcgcaaa ggagatcctg
     6721 atctgactga tgggctgcct gtatcgagtg gtgattttgt gccgagctgc cggtcgggga
     6781 gctgttggct ggctggtggc aggatatatt gtggtgtaaa caaattgacg cttagacaac
     6841 ttaataacac attgcggacg tttttaatgt actggggtgg atgcagtggg ccccac
//
