LOCUS       sequence-410039-        7505 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human SOX17 in mammalian cells, for lentivirus 
            generation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Weng M, Hu H, Graus MS, Tan DS, Gao Y, Ren S, Ho DHH, Langer J, 
            Holzner M, Huang Y, Ling GS, Lai CSW, Francois M, Jauch R
  TITLE     An engineered Sox17 induces somatic to neural stem cell fate 
            transitions independently from pluripotency reprogramming.
  JOURNAL   Sci Adv. 2023 Aug 25;9(34):eadh2501. doi: 10.1126/sciadv.adh2501. 
            Epub 2023 Aug 23.
  PUBMED    37611093
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2023-08-25"; volume: "9(34):eadh2501. doi"; pages: " 
            10.1126/sciadv.adh2501. Epub 2023 Aug 23"
COMMENT     Sequence Label: pHAGE2-TetO-hSOX17
FEATURES             Location/Qualifiers
     source          1..7505
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2251..2269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2293..2311
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2335..2353
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2377..2395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2419..2437
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2461..2479
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2547..2571
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3597..3614
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     CDS             3603..3620
                     /codon_start=1
                     /product="HQHQHQ metal affinity tag"
                     /label=HQ tag
                     /note="The HQ tag binds to nickel and other metal ions much
                     like the 6xHis tag, but may allow elution at lower 
                     concentrations of imidazole (Godat et al., 2008)."
                     /translation="HQHQHQ"
     misc_feature    3913..4501
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3966..3986)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4384..4395)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4631..4664)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4683..4863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4910..4928)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4996..5100
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5101..5169,5170..5961)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5319..5338)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6132..6720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6874..6891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7131..7152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7167..7172,7173..7190,7191..7197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7205..7221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7210..7232
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7229..7245
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7229..7245
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7295..7314
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcca
     2221 caccctaact gacacactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2281 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2341 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2401 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2461 tccctatcag tgatagagaa aagtgaaagt cgagctcggt acccgggtcg aggtaggcat
     2521 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2581 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctgcggcca
     2641 ccaccggtat gagcagcccg gatgcgggat acgccagtga cgaccagagc cagacccaga
     2701 gcgcgctgcc cgcggtgatg gccgggctgg gcccctgccc ctgggccgag tcgctgagcc
     2761 ccatcgggga catgaaggtg aagggcgagg cgccggcgaa cagcggagca ccggccgggg
     2821 ccgcgggccg agccaagggc gagtcccgta tccggcggcc gatgaacgct ttcatggtgt
     2881 gggctaagga cgagcgcaag cggctggcgc agcagaatcc agacctgcac aacgccgagt
     2941 tgagcaagat gctgggcaag tcgtggaagg cgctgacgct ggcggagaag cggcccttcg
     3001 tggaggaggc agagcggctg cgcgtgcagc acatgcagga ccaccccaac tacaagtacc
     3061 ggccgcggcg gcgcaagcag gtgaagcggc tgaagcgggt ggagggcggc ttcctgcacg
     3121 gcctggctga gccgcaggcg gccgcgctgg gccccgaggg cggccgcgtg gccatggacg
     3181 gcctgggcct ccagttcccc gagcagggct tccccgccgg cccgccgctg ctgcctccgc
     3241 acatgggcgg ccactaccgc gactgccaga gtctgggcgc gcctccgctc gacggctacc
     3301 cgttgcccac gcccgacacg tccccgctgg acggcgtgga ccccgacccg gctttcttcg
     3361 ccgccccgat gcccggggac tgcccggcgg ccggcaccta cagctacgcg caggtctcgg
     3421 actacgctgg ccccccggag cctcccgccg gtcccatgca cccccgactc ggcccagagc
     3481 ccgcgggtcc ctcgattccg ggcctcctgg cgccacccag cgcccttcac gtgtactacg
     3541 gcgcgatggg ctcgcccggg gcgggcggcg ggcgcggctt ccagatgcag ccgcaacacc
     3601 agcaccagca ccagcaccag caccaccccc cgggccccgg acagccgtcg ccccctccgg
     3661 aggcactgcc ctgccgggac ggcacggacc ccagtcagcc cgccgagctc ctcggggagg
     3721 tggaccgcac ggaatttgaa cagtatctgc acttcgtgtg caagcctgag atgggcctcc
     3781 cctaccaggg gcatgactcc ggtgtgaatc tccccgacag ccacggggcc atttcctcgg
     3841 tggtgtccga cgccagctcc gcggtatatt actgcaacta tcctgacgtg tgagtcgaca
     3901 tcgatagatc ctaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     3961 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4021 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4081 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4141 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4201 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4261 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     4321 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4381 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4441 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4501 cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4561 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc acctgcagga
     4621 caggcgcgcc ataacttcgt atagcataca ttatacgaag ttatctgctt tttgcttgta
     4681 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     4741 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     4801 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     4861 gcacccgggc gattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata
     4921 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4981 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5041 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5101 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5161 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5221 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5281 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5341 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     5401 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5461 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5521 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5581 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5641 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5701 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5761 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5821 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5881 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5941 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6001 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6241 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     6721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6901 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag
     6961 ctcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     7021 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg
     7081 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7141 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7201 ggaattgtga gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc
     7261 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7321 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
     7381 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
     7441 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
     7501 gtagt
//
