LOCUS       sequence-410127-        9612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo 
            recombineering and Bxb-1 for pInt incorporation. Has sacB for 
            counter selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Saunders SH, Ahmed AM
  TITLE     ORBIT for E. coli: Kilobase-scale oligonucleotide recombineering at 
            high throughput and high efficiency
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pHelper_NoMutL_V2_gentR
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      850..877
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(938..1471)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1660..1665,1666..1682,1683..1688))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        complement(1706..1810)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(1907..2785)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     CDS             complement(3006..3971)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     promoter        4871..4951
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     terminator      5915..6009
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     6012..6031
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             6132..7634
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      7669..7715
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             join(8156..8242,8243..9577)
                     /codon_start=1
                     /gene="Bacillus subtilis sacB"
                     /product="secreted levansucrase that renders bacterial 
                     growth sensitive to sucrose"
                     /label=SacB
                     /note="negative selection marker"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISH
                     ITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYH
                     IVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEW
                     SGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDG
                     KTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKA
                     YYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDE
                     IERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLK
                     MDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSV
                     VKDSILEQGQLTVNK"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tttttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccat gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctaga actagttgat
      841 tagctaagca gaaggccatc ctgacggatg gcctttttgc gtttaataga agttttaaat
      901 caatctaaag tatatatgag taaacttggt ctgacagtta ggtggcggta cttgggtcga
      961 tatcaaagtg catcacttct tcccgtatgc ccaactttgt atagagagcc actgcgggat
     1021 cgtcaccgta atctgcttgc acgtagatca cataagcacc aagcgcgttg gcctcatgct
     1081 tgaggagatt gatgagcgcg gtggcaatgc cctgcctccg gtgctcgccg gagactgcga
     1141 gatcatagat atagatctca ctacgcggct gctcaaactt gggcagaacg taagccgcga
     1201 gagcgccaac aaccgcttct tggtcgaagg cagcaagcgc gatgaatgtc ttactacgga
     1261 gcaagttccc gaggtaatcg gagtccggct gatgttggga gtaggtggct acgtctccga
     1321 actcacgacc gaaaagatca agagcagccc gcatggattt gacttggtca gggccgagcc
     1381 tacatgtgcg aatgatgccc atacttgagc cacctaactt tgttttaggg cgactgccct
     1441 gctgcgtaac atcgttgctg ctgcgtaaca tcgttgctgc tccataacat caaacatcga
     1501 cccacggcgt aacgcgcttg ctgcttggat gcccgaggca tagactgtac aaaaaaacag
     1561 tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
     1621 ctggaccagt tgcgtgagcg catacgctac ttgcattaca gtttacgaac cgaacaggct
     1681 tatgtcaatt cggttgaata ctcatactct tcctttttca atattattga agcatttatc
     1741 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     1801 gggttccgcg cacatttccc cgaaaagtgg gtaataactg atataattaa attgaagctc
     1861 taatttgtga gtttagtata catgcattta cttataatac agttttttat gacaacttga
     1921 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     1981 tgcatttttt aaatacccgc gagaagtaga gttgatcgtc aaaaccaaca ttgcgaccga
     2041 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     2101 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     2161 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     2221 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     2281 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     2341 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     2401 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     2461 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     2521 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     2581 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     2641 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     2701 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     2761 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga gaagaaacca
     2821 attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa
     2881 ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga
     2941 caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc
     3001 acggctcaag ccacttcctt tttgcattga cgcagggtgt cggaaggcaa ctcgccgaac
     3061 gcgctcctat agttttcagc gaagcgtccc aaatgtaaga agccgtagtc tagggctatc
     3121 tcagttatac tacgcacatt ggcactggga tcgttcaagc aggcgcggat gctttcgagc
     3181 ttgcggttgc ggatgtagtt cttcggcgtg gtgccggcgt gcttctcgaa caaattgtag
     3241 agcgagcgtg gactcatcat cgccagctcc gctaaccgct caaggctgat attccgtttg
     3301 agattctcct caatgaattg aacgactcgc tcgaaagacg ggttaccttt gctgaaaatt
     3361 tcacggctga cattgctgcc cagcatttcg agcagcttgg aagcgatgat ccccgcatag
     3421 tgctcttgga cccgaggcat cgactttgta tgttccgctt cgtcacaaac taacccgagt
     3481 agattgataa agccatcgag ttgctggaga ttgtgtcgcg cggcgaaacg gataccctcc
     3541 ctcggcttgt gccaattgtt gtcactgcac gcccgatcaa ggaccactga gggcaattta
     3601 acgataaatt tctcgcaatc ttctgaatag gtcaggtcgg cttggtcatc cggattgagc
     3661 agcaatagtt cgcccggcgc aaaatagtgc tcctggccat ggccacgcca caggcaatgg
     3721 cctttgagta ttatttgcag atgataacag gtctctaatc caggcgagat taccctcacg
     3781 ctaccgccgt agctgattcg acacagatcg aggcatccga agattctgtg gtgcagcctg
     3841 cctgccgggc gcccgccctt gggcaggcga atagagtgcg taccgacata ctggttaaca
     3901 taatcggaga ctgcataggg ctcggcgtgg acgaagatct gacttttctc gttcaataag
     3961 caaaaatcca tagttcacgg ttctcttatt ttaatgtggg ctgcttggtg tgatgtagaa
     4021 aggcgccaag tcgatgaaaa tgcatctcga cgtgatgcgt atacgggtta cccccattgc
     4081 cacgttgcgc catccttttt gcaatcagtg accacttttc caagcaaaaa taacgccaag
     4141 cagaacgaag acgttctttt taagaagcga gaacaccaga agttcgtgct gtcggggcat
     4201 ggggcgacga attggcggat aaaggggatc tgctggatat tacggccttt ttaaagaccg
     4261 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     4321 atgctcatcc gtaattacgt atggcaatga aagacggtga gctggtgata tgggatagtg
     4381 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     4441 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     4501 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca
     4561 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     4621 cccccgtttt caccatgcat gggaattagc ttgatctgac caacgaccgg tagcggagct
     4681 atccaacggc ggtataccag gaaaacacac agcaggtaca tcagaacagt accatgactg
     4741 aagaacaaat agttttttcc tgatccataa agcagaacgg cctgctccat gacaaatctg
     4801 gctccccaac taatgcccca tgcagccagc ataaccagca taaagtgcag tgtccggttt
     4861 gatagggata agtccagcct tgcaagaagc ggatacagga gtgcaaaaaa tggctatctc
     4921 tagtaaggcc taccccttag gctttatgca acagaaacaa taataatgga gtcatgacca
     4981 tgcctaggcc gcgggccgcg cgaattcaga aggagaatat accatgaacc aaatcgtgaa
     5041 gttcactgac gactctggcc tggcggttca agttactcca gacgatgttc gccgttatat
     5101 ctgtgagaac gctactgaaa aagaggtggg cctctttctg caactctgtc agactcaacg
     5161 tctgaatccg tttgtgaaag acgcttacct ggtgaaatac ggcggtgctc cagcttctat
     5221 gattacttcc tatcaagttt ttaaccgtcg cgcgtgtcgt gatgctaact atgatggtat
     5281 caaatctggt gtggttgttc tgcgtgacgg tgatgttgtg cataaacgtg gtgctgcgtg
     5341 ctacaaaaag gcgggtgagg agctcatcgg tggttgggcg gaagttcgct ttaaggatgg
     5401 ccgcgagact gcgtatgctg aggtggcgct cgacgactat tccaccggca aatctaattg
     5461 ggcgaaaatg ccgggtgtta tgatcgaaaa atgcgcgaag gctgctgctt ggcgcctcgc
     5521 gttcccggac acttttcagg gcatgtacgc tgcggaggaa atggatcaag cgcaacagcc
     5581 agaacaggtg cgcgctcagg cggagcaacc agtggatctc cagccaatcc gcgaactctt
     5641 caagccatat tgcgaacact tcggcatcac tccggctgag ggtatgactg ctgtttgtgg
     5701 tgcggtgggc gctgaaggca tgcactctat gaccgagcag caagctcgcc gtgctcgcgc
     5761 ttggatggag gaagaaatgg ctgcgccagc tgtggaagcg gagtatgagg ttgttgacga
     5821 gggcgaggtg ttttaaggat cctctagagt cgacctgcag gcatgcaagc ttgcggccgc
     5881 gtcgtgactg ggaaaaccct ggcgactagt cttggactcc tgttgataga tccagtaatg
     5941 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     6001 ggtgagaatc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
     6061 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggaattcg agctctaagg
     6121 aggaaaaaca tatgagagcc ctggtagtca tccgcctgtc ccgcgtcacc gatgctacga
     6181 cttcaccgga gcgtcagctg gagtcttgcc agcagctctg cgcccagcgc ggctgggacg
     6241 tcgtcggggt agcggaggat ctggacgtct ccggggcggt cgatccgttc gaccggaagc
     6301 gcagaccgaa cctggcccgg tggctagcgt tcgaggagca accgttcgac gtgatcgtgg
     6361 cgtaccgggt agaccggttg acccgatcga tccggcatct gcagcagctg gtccactggg
     6421 ccgaggacca caagaagctg gtcgtctccg cgaccgaagc gcacttcgat acgacgacgc
     6481 cgtttgcggc ggtcgtcatc gcgcttatgg gaacggtggc gcagatggaa ttagaagcga
     6541 tcaaagagcg gaaccgttcg gctgcgcatt tcaatatccg cgccgggaaa taccgaggat
     6601 ccctgccgcc gtggggatac ctgcctacgc gcgtggacgg ggagtggcgg ctggtgccgg
     6661 accctgtgca gcgagagcgc atcctcgagg tgtatcaccg cgtcgtcgac aaccacgagc
     6721 cgctgcacct ggtggcccac gacctgaacc ggcgtggtgt cctgtcgccg aaggactact
     6781 tcgcgcagct gcaaggccgc gagccgcagg gccgggagtg gtcggctacc gcgctgaagc
     6841 gatcgatgat ctccgaggcg atgctcgggt acgcgactct gaacggtaag accgtccgag
     6901 acgacgacgg agccccgctg gtgcgggctg agccgatcct gacccgtgag cagctggagg
     6961 cgctgcgcgc cgagctcgtg aagacctccc gggcgaagcc cgcggtgtct accccgtcgc
     7021 tgctgctgcg ggtgttgttc tgcgcggtgt gcggggagcc cgcgtacaag ttcgccgggg
     7081 gaggacgtaa gcacccgcgc taccgctgcc gctcgatggg gttcccgaag cactgcggga
     7141 acggcacggt ggcgatggcc gagtgggacg cgttctgcga ggagcaggtg ctggatctgc
     7201 tcggggacgc ggagcgtctg gagaaagtct gggtagccgg ctcggactcc gcggtcgaac
     7261 tcgcggaggt gaacgcggag ctggtggacc tgacgtcgct gatcggctcc ccggcctacc
     7321 gggccggctc tccgcagcga gaagcactgg atgcccgtat tgcggcgctg gccgcgcggc
     7381 aagaggagct ggagggtcta gaggctcgcc cgtctggctg ggagtggcgc gagaccgggc
     7441 agcggttcgg ggactggtgg cgggagcagg acaccgcggc aaagaacacc tggcttcggt
     7501 cgatgaacgt tcggctgacg ttcgacgtcc gcggcgggct gactcgcacg atcgacttcg
     7561 gggatctgca ggagtacgag cagcatctca ggctcggcag cgtggtcgaa cggctacaca
     7621 ccgggatgtc gtaggttaac aactagcgta cgatcgactg ccaggcatca aataaaacga
     7681 aaggctcagt cgaaagactg ggcctttcgt tttatgccat catggccgcg ggacgtcggg
     7741 ggggggggga aagccacgtt gtgtctcaaa atctctgatg ttacattgca caagatatgc
     7801 aacagaaact ataaaaaata cagagaatga aaagaaacag atagattttt tagttcttta
     7861 ggcccgtagt ctgcaaatcc ttttatgatt ttctatcaaa caaaagagga aaatagacca
     7921 gttgcaatcc aaacgagagt ctaatagaat gaggtcgaaa agtaaatcgc gcgggtttgt
     7981 tactgataaa gcaggcaaga cctaaaatgt gtaaagggca aagtgtatac tttggcgtca
     8041 ccccttacat attttaggtc tttttttatt gtgcgtaact aacttgccat cttcaaacag
     8101 gagggctgga agaagcagac cgctaacaca gtacataaaa aaggagacat gaacgatgaa
     8161 catcaaaaag tttgcaaaac aagcaacagt attaaccttt actaccgcac tgctggcagg
     8221 aggcgcaact caagcgtttg cgaaagaaac gaaccaaaag ccatataagg aaacatacgg
     8281 catttcccat attacacgcc atgatatgct gcaaatccct gaacagcaaa aaaatgaaaa
     8341 atatcaagtt cctgaattcg attcgtccac aattaaaaat atctcttctg caaaaggcct
     8401 ggacgtttgg gacagctggc cattacaaaa cgctgacggc actgtcgcaa actatcacgg
     8461 ctaccacatc gtctttgcat tagccggaga tcctaaaaat gcggatgaca catcgattta
     8521 catgttctat caaaaagtcg gcgaaacttc tattgacagc tggaaaaacg ctggccgcgt
     8581 ctttaaagac agcgacaaat tcgatgcaaa tgattctatc ctaaaagacc aaacacaaga
     8641 atggtcaggt tcagccacat ttacatctga cggaaaaatc cgtttattct acactgattt
     8701 ctccggtaaa cattacggca aacaaacact gacaactgca caagttaacg tatcagcatc
     8761 agacagctct ttgaacatca acggtgtaga ggattataaa tcaatctttg acggtgacgg
     8821 aaaaacgtat caaaatgtac agcagttcat cgatgaaggc aactacagct caggcgacaa
     8881 ccatacgctg agagatcctc actacgtaga agataaaggc cacaaatact tagtatttga
     8941 agcaaacact ggaactgaag atggctacca aggcgaagaa tctttattta acaaagcata
     9001 ctatggcaaa agcacatcat tcttccgtca agaaagtcaa aaacttctgc aaagcgataa
     9061 aaaacgcacg gctgagttag caaacggcgc tctcggtatg attgagctaa acgatgatta
     9121 cacactgaaa aaagtgatga aaccgctgat tgcatctaac acagtaacag atgaaattga
     9181 acgcgcgaac gtctttaaaa tgaacggcaa atggtacctg ttcactgact cccgcggatc
     9241 aaaaatgacg attgacggca ttacgtctaa cgatatttac atgcttggtt atgtttctaa
     9301 ttctttaact ggcccataca agccgctgaa caaaactggc cttgtgttaa aaatggatct
     9361 tgatcctaac gatgtaacct ttacttactc acacttcgct gtacctcaag cgaaaggaaa
     9421 caatgtcgtg attacaagct atatgacaaa cagaggattc tacgcagaca aacaatcaac
     9481 gtttgcgcca agcttcctgc tgaacatcaa aggcaagaaa acatctgttg tcaaagacag
     9541 catccttgaa caaggacaat taacagttaa caaataagcg tcagaccccg tagaaaagat
     9601 caaaggatct tc
//
