LOCUS       sequence-410203-       11133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enables constitutive expression of N-terminal Split-TurboID-fused 
            PML (isoform IVa; WT); to perform SUMO-ID; selection with 
            blasticidin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11133)
  AUTHORS   Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, 
            Cortazar AR, Perez C, Azkargorta M, Iloro I, Carracedo A, Aransay 
            AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD
  TITLE     Identification of proximal SUMO-dependent interactors using SUMO-ID.
  JOURNAL   Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6.
  PUBMED    34795231
REFERENCE   2  (bases 1 to 11133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-18"; volume: "12(1):6671. doi"; pages: " 
            10.1038/s41467-021-26807-6"
COMMENT     Sequence Label: Lenti-EFS-NTID194-GSQ-PMLivaWT-P2A-BLAST
FEATURES             Location/Qualifiers
     source          1..11133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2654..2865
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2873..2882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2882..2905
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5363..5419
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5420..5818
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    5846..6434
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5899..5919)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6317..6328)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6437..6453)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6438..6454)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6959..7139
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7165..7182)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7171..7395
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7441..7869
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7528..7547)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7738..7759
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7878..7898)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8043..8177
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8104..8123
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8239..8286
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8305..8679
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8809..8930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8846..8865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8900..8919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8979..8995)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8979..8995)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    8999..9023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9027..9033,9034..9051,9052..9057))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9072..9093
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9210..9227)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9381..9969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9461..9480)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10140..10931,10932..11000))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10763..10782
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11001..11105)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta attagctagc taggtcttga aaggagtggg aattggctcc
     2641 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2701 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2761 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2821 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg gtgccaccat
     2881 ggactacaaa gacgatgacg acaagggctc taaagacaat actgtgcctc tgaagctgat
     2941 cgctctcctg gctaatggcg agttccatag tggcgaacag ctgggagaaa ccctgggcat
     3001 gtccagggcc gctatcaaca agcacattca gactctgcgc gactggggcg tggacgtgtt
     3061 caccgtgccc ggaaagggct actctctgcc cgagcctatc ccgctgctga acgctaaaca
     3121 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtgg actccaccaa
     3181 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     3241 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     3301 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     3361 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     3421 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     3481 cggaataaca gggcgcgccg gcgggggatc ttccggaggc gggggatctt ccggaggcgg
     3541 gggatcttcc ggaggcgggc aaatttctta cgcaagtaga ggggaattca tggagcctgc
     3601 acccgcccga tctccgaggc cccagcagga ccccgcccgg ccccaggagc ccaccatgcc
     3661 tccccccgag accccctctg aaggccgcca gcccagcccc agccccagcc ctacagagcg
     3721 agcccccgct tcggaggagg agttccagtt tctgcgctgc cagcaatgcc aggcggaagc
     3781 caagtgcccg aagctgctgc cttgtctgca cacgctgtgc tcaggatgcc tggaggcgtc
     3841 gggcatgcag tgccccatct gccaggcgcc ctggccccta ggtgcagaca cacccgccct
     3901 ggataacgtc tttttcgaga gtctgcagcg gcgcctgtcg gtgtaccggc agattgtgga
     3961 tgcgcaggct gtgtgcaccc gctgcaaaga gtcggccgac ttctggtgct ttgagtgcga
     4021 gcagctcctc tgcgccaagt gcttcgaggc acaccagtgg ttcctcaagc acgaggcccg
     4081 gcccctagca gagctgcgca accagtcggt gcgtgagttc ctggacggca cccgcaagac
     4141 caacaacatc ttctgctcca accccaacca ccgcacccct acgctgacca gcatctactg
     4201 ccgaggatgt tccaagccgc tgtgctgctc gtgcgcgctc cttgacagca gccacagtga
     4261 gctcaagtgc gacatcagcg cagagatcca gcagcgacag gaggagctgg acgccatgac
     4321 gcaggcgctg caggagcagg atagtgcctt tggcgcggtt cacgcgcaga tgcacgcggc
     4381 cgtcggccag ctgggccgcg cgcgtgccga gaccgaggag ctgatccgcg agcgcgtgcg
     4441 ccaggtggta gctcacgtgc gggctcagga gcgcgagctg ctggaggctg tggacgcgcg
     4501 gtaccagcgc gactacgagg agatggccag tcggctgggc cgcctggatg ctgtgctgca
     4561 gcgcatccgc acgggcagcg cgctggtgca gaggatgaag tgctacgcct cggaccagga
     4621 ggtgctggac atgcacggtt tcctgcgcca ggcgctctgc cgcctgcgcc aggaggagcc
     4681 ccagagcctg caagctgccg tgcgcaccga tggcttcgac gagttcaagg tgcgcctgca
     4741 ggacctcagc tcttgcatca cccaggggaa agatgcagct gtatccaaga aagccagccc
     4801 agaggctgcc agcactccca gggaccctat tgacgttgac ctggatgtct ccaatacaac
     4861 gacagcccag aagaggaagt gcagccagac ccagtgcccc aggaaggtca tcaagatgga
     4921 gtctgaggag gggaaggagg caaggttggc tcggagctcc ccggagcagc ccaggcccag
     4981 cacctccaag gcagtctcac caccccacct ggatggaccg cctagcccca ggagccccgt
     5041 cataggaagt gaggtcttcc tgcccaacag caaccacgtg gccagtggcg ccggggaggc
     5101 agaggaacgc gttgtggtga tcagcagctc ggaagactca gatgccgaaa actcgtcctc
     5161 ccgagagctg gatgacagca gcagtgagtc cagtgacctc cagctggaag gccccagcac
     5221 cctcagggtc ctggacgaga accttgctga cccccaagca gaagacagac ctctggtttt
     5281 ctttgacctc aagattgaca atgaaagtgg gttctcctgg ggctaccccc acccctttct
     5341 aattggcggc cgcggatccg gcgcaacaaa cttctctctg ctgaaacaag ccggagatgt
     5401 cgaagagaat cctggaccga tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     5461 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     5521 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     5581 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     5641 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     5701 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     5761 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaga
     5821 ataattcgat atcaagctta tcggtaatca acctctggat tacaaaattt gtgaaagatt
     5881 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     5941 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     6001 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     6061 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     6121 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     6181 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     6241 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     6301 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     6361 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     6421 ggccgcctcc ccgcatcgat accgtcgacc tcgagaccta gaaaaacatg gagcaatcac
     6481 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     6541 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     6601 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6661 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgattg
     6721 gcagaactac acaccagggc cagggatcag atatccactg acctttggat ggtgctacaa
     6781 gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga acacccgctt
     6841 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat tagagtggag
     6901 gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg actgtactgg
     6961 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     7021 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     7081 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     7141 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     7201 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     7261 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     7321 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     7381 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     7441 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     7501 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     7561 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     7621 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     7681 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     7741 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     7801 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     7861 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     7921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     7981 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     8041 agtcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8101 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     8161 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     8221 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     8281 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     8341 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     8401 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     8461 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     8521 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     8581 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     8641 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     8701 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     8761 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     8821 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     8881 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     8941 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtgg
     9001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     9061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     9121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     9181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     9241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     9301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     9361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     9421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     9841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9901 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9961 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10021 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    10081 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    10141 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    10201 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    10261 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    10321 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    10381 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    10441 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10501 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10561 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10621 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10681 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10741 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10801 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10861 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10921 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10981 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    11041 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    11101 ccgcgcacat ttccccgaaa agtgccacct gac
//
