LOCUS       sequence-410204-       11475 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enables constitutive expression of N-terminal TurboID-fused PML 
            (isoform IVa; WT); selection with blasticidin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11475)
  AUTHORS   Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, 
            Cortazar AR, Perez C, Azkargorta M, Iloro I, Carracedo A, Aransay 
            AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD
  TITLE     Identification of proximal SUMO-dependent interactors using SUMO-ID.
  JOURNAL   Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6.
  PUBMED    34795231
REFERENCE   2  (bases 1 to 11475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-18"; volume: "12(1):6671. doi"; pages: " 
            10.1038/s41467-021-26807-6"
COMMENT     Sequence Label: Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST
FEATURES             Location/Qualifiers
     source          1..11475
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1684..1917
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2102..2146
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2654..2865
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2873..2882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2882..2905
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2912..3868
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             5705..5761
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5762..6160
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6188..6776
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6241..6261)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6659..6670)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6779..6795)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6780..6796)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7301..7481
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7507..7524)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7513..7737
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7783..8211
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7870..7889)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8080..8101
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8220..8240)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8385..8519
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8446..8465
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8581..8628
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8647..9021
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9151..9272
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9188..9207)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9242..9261
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9321..9337)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9321..9337)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9334..9356)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9345..9361
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9369..9375,9376..9393,9394..9399))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9414..9435
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9552..9569)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9723..10311)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9803..9822)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10482..11273,11274..11342))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11343..11447)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta attagctagc taggtcttga aaggagtggg aattggctcc
     2641 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     2701 gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     2761 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     2821 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggaccg gtgccaccat
     2881 ggactacaaa gacgatgacg acaagggctc taaagacaat actgtgcctc tgaagctgat
     2941 cgctctcctg gctaatggcg agttccatag tggcgaacag ctgggagaaa ccctgggcat
     3001 gtccagggcc gctatcaaca agcacattca gactctgcgc gactggggcg tggacgtgtt
     3061 caccgtgccc ggaaagggct actctctgcc cgagcctatc ccgctgctga acgctaaaca
     3121 gattctggga cagctggacg gcgggagcgt ggcagtcctg cctgtggtcg actccaccaa
     3181 tcagtacctg ctggatcgaa tcggcgagct gaagagtggg gatgcttgca ttgcagaata
     3241 tcagcaggca gggagaggaa gcagagggag gaaatggttc tctccttttg gagctaacct
     3301 gtacctgagt atgttttggc gcctgaagcg gggaccagca gcaatcggcc tgggcccggt
     3361 catcggaatt gtcatggcag aagcgctgcg aaagctggga gcagacaagg tgcgagtcaa
     3421 atggcccaat gacctgtatc tgcaggatag aaagctggca ggcatcctgg tggagctggc
     3481 cggaataaca ggcgatgctg cacagatcgt cattggcgcc gggattaacg tggctatgag
     3541 gcgcgtggag gaaagcgtgg tcaatcaggg ctggatcaca ctgcaggaag cagggattaa
     3601 cctggacagg aatactctgg ccgctacgct gatccgagag ctgcgggcag ccctggaact
     3661 gttcgagcag gaaggcctgg ctccatatct gccacggtgg gagaagctgg ataacttcat
     3721 caatagaccc gtgaagctga tcattgggga caaagagatt ttcgggatta gccgggggat
     3781 tgataaacag ggagccctgc tgctggaaca ggacggagtt atcaaaccct ggatgggcgg
     3841 agaaatcagt ctgcggtctg ccgaaaaggg gcgcgccggc gggggatctt ccggaggcgg
     3901 gcaaatttct tacgcaagta gaggggaatt catggagcct gcacccgccc gatctccgag
     3961 gccccagcag gaccccgccc ggccccagga gcccaccatg cctccccccg agaccccctc
     4021 tgaaggccgc cagcccagcc ccagccccag ccctacagag cgagcccccg cttcggagga
     4081 ggagttccag tttctgcgct gccagcaatg ccaggcggaa gccaagtgcc cgaagctgct
     4141 gccttgtctg cacacgctgt gctcaggatg cctggaggcg tcgggcatgc agtgccccat
     4201 ctgccaggcg ccctggcccc taggtgcaga cacacccgcc ctggataacg tctttttcga
     4261 gagtctgcag cggcgcctgt cggtgtaccg gcagattgtg gatgcgcagg ctgtgtgcac
     4321 ccgctgcaaa gagtcggccg acttctggtg ctttgagtgc gagcagctcc tctgcgccaa
     4381 gtgcttcgag gcacaccagt ggttcctcaa gcacgaggcc cggcccctag cagagctgcg
     4441 caaccagtcg gtgcgtgagt tcctggacgg cacccgcaag accaacaaca tcttctgctc
     4501 caaccccaac caccgcaccc ctacgctgac cagcatctac tgccgaggat gttccaagcc
     4561 gctgtgctgc tcgtgcgcgc tccttgacag cagccacagt gagctcaagt gcgacatcag
     4621 cgcagagatc cagcagcgac aggaggagct ggacgccatg acgcaggcgc tgcaggagca
     4681 ggatagtgcc tttggcgcgg ttcacgcgca gatgcacgcg gccgtcggcc agctgggccg
     4741 cgcgcgtgcc gagaccgagg agctgatccg cgagcgcgtg cgccaggtgg tagctcacgt
     4801 gcgggctcag gagcgcgagc tgctggaggc tgtggacgcg cggtaccagc gcgactacga
     4861 ggagatggcc agtcggctgg gccgcctgga tgctgtgctg cagcgcatcc gcacgggcag
     4921 cgcgctggtg cagaggatga agtgctacgc ctcggaccag gaggtgctgg acatgcacgg
     4981 tttcctgcgc caggcgctct gccgcctgcg ccaggaggag ccccagagcc tgcaagctgc
     5041 cgtgcgcacc gatggcttcg acgagttcaa ggtgcgcctg caggacctca gctcttgcat
     5101 cacccagggg aaagatgcag ctgtatccaa gaaagccagc ccagaggctg ccagcactcc
     5161 cagggaccct attgacgttg acctggatgt ctccaataca acgacagccc agaagaggaa
     5221 gtgcagccag acccagtgcc ccaggaaggt catcaagatg gagtctgagg aggggaagga
     5281 ggcaaggttg gctcggagct ccccggagca gcccaggccc agcacctcca aggcagtctc
     5341 accaccccac ctggatggac cgcctagccc caggagcccc gtcataggaa gtgaggtctt
     5401 cctgcccaac agcaaccacg tggccagtgg cgccggggag gcagaggaac gcgttgtggt
     5461 gatcagcagc tcggaagact cagatgccga aaactcgtcc tcccgagagc tggatgacag
     5521 cagcagtgag tccagtgacc tccagctgga aggccccagc accctcaggg tcctggacga
     5581 gaaccttgct gacccccaag cagaagacag acctctggtt ttctttgacc tcaagattga
     5641 caatgaaagt gggttctcct ggggctaccc ccaccccttt ctaattggcg gccgcggatc
     5701 cggcgcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     5761 gatggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     5821 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     5881 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     5941 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     6001 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     6061 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     6121 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaataattcg atatcaagct
     6181 tatcggtaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6241 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6301 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6361 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6421 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6481 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6541 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6601 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6661 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6721 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     6781 ataccgtcga cctcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     6841 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     6901 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6961 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7021 tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg
     7081 gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca
     7141 agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct
     7201 gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc
     7261 atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca
     7321 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7381 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7441 atccctcaga cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct
     7501 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
     7561 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
     7621 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
     7681 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
     7741 ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg
     7801 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     7861 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     7921 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     7981 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     8041 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     8101 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     8161 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg
     8221 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8281 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8341 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8401 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8461 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8521 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
     8581 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
     8641 taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga
     8701 gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga ggacgacttc
     8761 gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg
     8821 ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg
     8881 tcggaggtcg tgtccacgaa cttccgggac gcctccgggc cggccatgac cgagatcggc
     8941 gagcagccgt gggggcggga gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc
     9001 gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc cgccttctat
     9061 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9121 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     9181 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9241 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9301 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9361 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9421 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9541 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9601 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9661 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9721 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     9781 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     9841 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     9901 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     9961 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10021 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
    10081 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
    10141 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
    10201 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
    10261 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
    10321 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
    10381 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
    10441 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
    10501 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    10561 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    10621 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    10681 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    10741 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
    10801 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    10861 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    10921 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    10981 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    11041 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
    11101 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    11161 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    11221 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    11281 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    11341 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    11401 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    11461 aaagtgccac ctgac
//
