LOCUS       sequence-410209-       13085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Enables inducible expression of C-terminal Split-TurboID-fused 
            SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection 
            with puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13085)
  AUTHORS   Barroso-Gomila O, Trulsson F, Muratore V, Canosa I, Merino-Cacho L, 
            Cortazar AR, Perez C, Azkargorta M, Iloro I, Carracedo A, Aransay 
            AM, Elortza F, Mayor U, Vertegaal ACO, Barrio R, Sutherland JD
  TITLE     Identification of proximal SUMO-dependent interactors using SUMO-ID.
  JOURNAL   Nat Commun. 2021 Nov 18;12(1):6671. doi: 10.1038/s41467-021-26807-6.
  PUBMED    34795231
REFERENCE   2  (bases 1 to 13085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2021-11-18"; volume: "12(1):6671. doi"; pages: " 
            10.1038/s41467-021-26807-6"
COMMENT     Sequence Label: TRIPZ-CTID195-GSQ-SUMO1nc-PURO
FEATURES             Location/Qualifiers
     source          1..13085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2063..2180
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2245..2619)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     regulatory      2616..2625
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2629..2647)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(2639..2686)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     protein_bind    2722..2740
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2757..2775
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2793..2811
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2829..2847
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2864..2882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2900..2918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2930..2968
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2965..2989
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2998..3007
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3007..3036
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     promoter        3836..5046
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     4883..4902
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(5059..5092)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             5099..5806
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(5807..5840)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     misc_feature    5863..6435
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6030..6047)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6257..6276
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6438..7040
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6937..6957
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    7056..7644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7109..7129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7527..7538)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7851..8084
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8185..8202)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8191..8415
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8461..8889
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8548..8567)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8758..8779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8898..8918)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8903..9232
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9083..9218
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9145..9164
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    10440..10561
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10610..10626)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10610..10626)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10623..10645)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10634..10650
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10658..10664,10665..10682,10683..10688))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10703..10724
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10841..10858)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11012..11600)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11092..11111)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11771..12562,12563..12631))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12632..12736)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12784..12918
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     2041 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     2101 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     2161 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttag
     2221 taccgggccc gctctagtcc ggaatcagtc ctgctcctcg gccacgaagt gcacgcagtt
     2281 gccggccggg tcgcgcaggg cgaactcccg cccccacggc tgctcgccga tctcggtcat
     2341 ggccggcccg gaggcgtccc ggaagttcgt ggacacgacc tccgaccact cggcgtacag
     2401 ctcgtccagg ccgcgcaccc acacccaggc cagggtgttg tccggcacca cctggtcctg
     2461 gaccgcgctg atgaacaggg tcacgtcgtc ccggaccaca ccggcgaagt cgtcctccac
     2521 gaagtcccgg gagaacccga gccggtcggt ccagaactcg accgctccgg cgacgtcgcg
     2581 cgcggtgagc accggaacgg cactggtcaa cttggccatg gtggccctcc tatagtgagt
     2641 cgtattatac tatgccgata tactatgccg atgattaatt gtcaacacgt gctgcaggtc
     2701 cgaggttcta gacgagttta ctccctatca gtgatagaga acgatgtcga gtttactccc
     2761 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
     2821 gagtttactc cctatcagtg atagagaacg tatgtcgagt ttatccctat cagtgataga
     2881 gaacgtatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag gtaggcgtgt
     2941 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg caccggtgcc
     3001 accatggaac aaaaactcat ctcagaagag gatctgggct ctggcggcga tgctgcacag
     3061 atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag cgtggtcaat
     3121 cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac tctggccgct
     3181 acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg cctggctcca
     3241 tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa gctgatcatt
     3301 ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc cctgctgctg
     3361 gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg gtctgccgaa
     3421 aaggggcgcg ccggcggggg atcttccgga ggcgggggat cttccggagg cgggcaaatt
     3481 tcttacgcaa gtagagggga attcatgtct gaccaggagg caaaaccttc aactgaggac
     3541 ttgggggata agaaggaagg tgaatatatt aaactcaaag tcattggaca ggatagcagt
     3601 gagattcact tcaaagtgaa aatgacaaca catctcaaga aactcaaaga atcatactgt
     3661 caaagacagg gtgttccaat gaatagcctc aggtttctct ttgagggtca gagaattgct
     3721 gataatcata ctccaaaaga actgggaatg gaggaagaag atgtgattga agtttatcag
     3781 gaacctacgg gtggttaaac gcgtaataaa ataggcctcc gcgccgggtt ttggcgcctc
     3841 cgcgccgggt tttggcgcct cccgcgggcg cccccctcct cacggcgagc gctgccacgt
     3901 cagacgaagg gcgcagcgag cgtcctgatc cttccgcccg gacgctcagg acagcggccc
     3961 gctgctcata agactcggcc ttagaacccc agtatcagca gaaggacatt ttaggacggg
     4021 acttgggtga ctctagggca ctggttttct ttccagagag cggaacaggc gaggaaaagt
     4081 agtcccttct cggcgattct gcggagggat ctccgtgggg cggtgaacgc cgatgattat
     4141 ataaggacgc gccgggtgtg gcacagctag ttccgtcgca gccgggattt gggtcgcggt
     4201 tcttgtttgt ggatcgctgt gatcgtcact tggtgagtag cgggctgctg ggctggccgg
     4261 ggctttcgtg gccgccgggc cgctcggtgg gacggaagcg tgtggagaga ccgccaaggg
     4321 ctgtagtctg ggtccgcgag caaggttgcc ctgaactggg ggttgggggg agcgcagcaa
     4381 aatggcggct gttcccgagt cttgaatgga agacgcttgt gaggcgggct gtgaggtcgt
     4441 tgaaacaagg tggggggcat ggtgggcggc aagaacccaa ggtcttgagg ccttcgctaa
     4501 tgcgggaaag ctcttattcg ggtgagatgg gctggggcac catctgggga ccctgacgtg
     4561 aagtttgtca ctgactggag aactcggttt gtcgtctgtt gcgggggcgg cagttatggc
     4621 ggtgccgttg ggcagtgcac ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg
     4681 tcacccgttc tgttggctta taatgcaggg tggggccacc tgccggtagg tgtgcggtag
     4741 gcttttctcc gtcgcaggac gcagggttcg ggcctagggt aggctctcct gaatcgacag
     4801 gcgccggacc tctggtgagg ggagggataa gtgaggcgtc agtttctttg gtcggtttta
     4861 tgtacctatc ttcttaagta gctgaagctc cggttttgaa ctatgcgctc ggggttggcg
     4921 agtgtgtttt gtgaagtttt ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt
     4981 aattttcagt gttagactag taaattgtcc gctaaattct ggccgttttt ggcttttttg
     5041 ttagacgcta gcggatccat aacttcgtat agtatacatt atacgaagtt atgccaccat
     5101 gtctaggctg gacaagagca aagtcataaa cggagctctg gaattactca atggtgtcgg
     5161 tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc agcctaccct
     5221 gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg agatgctgga
     5281 caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact ttctgcggaa
     5341 caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta aagtgcatct
     5401 cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg cgttcctgtg
     5461 tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg gccactttac
     5521 actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa gagagacacc
     5581 taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg accggcaggg
     5641 agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg agaaacagct
     5701 aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag acatgctccc
     5761 agccgatgcc cttgacgatt ttgaccttga catgctcccc gggtaaataa cttcgtatag
     5821 tatacattat acgaagttat ggatccgcgg ccgcaaattc cgcccctctc cctccccccc
     5881 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     5941 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     6001 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     6061 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     6121 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     6181 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     6241 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     6301 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     6361 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     6421 aaaaacacga taataccatg gccaccgagt acaagcccac ggtgcgcctc gccacccgcg
     6481 acgacgtccc ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     6541 gccacaccgt cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     6601 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     6661 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggctcgc
     6721 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     6781 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     6841 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     6901 ctggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     6961 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     7021 cccgcaagcc cggtgcctga gttcgcgtct ggaacaatca acctctggat tacaaaattt
     7081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     7141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     7201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     7261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     7321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     7381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     7441 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     7501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     7561 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     7621 tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc tagaaaaaca
     7681 tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct ggctagaagc
     7741 acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac
     7801 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac tggaagggct
     7861 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     7921 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7981 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     8041 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     8101 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg
     8161 acattgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     8221 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     8281 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     8341 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     8401 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     8461 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     8521 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     8581 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     8641 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     8701 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     8761 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     8821 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     8881 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     8941 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     9001 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     9061 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     9121 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     9181 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     9241 gggagcttgt atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac
     9301 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
     9361 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
     9421 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
     9481 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggaattaa ttcagcgaga
     9541 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     9601 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     9661 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     9721 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     9781 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     9841 aggactgccc cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga
     9901 cggacaatgg ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc
     9961 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
    10021 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
    10081 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
    10141 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
    10201 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
    10261 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaatag cacgtgctac
    10321 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
    10381 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
    10441 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
    10501 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
    10561 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
    10621 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10681 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10741 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10801 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10861 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10921 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10981 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    11041 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    11101 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    11161 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    11221 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    11281 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11341 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11401 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11461 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11521 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11581 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11641 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11701 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11761 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11821 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11881 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11941 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    12001 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    12061 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    12121 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    12181 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    12241 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    12301 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    12361 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12421 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12481 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12541 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12601 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12661 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12721 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag
    12781 atcaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
    12841 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
    12901 tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc
    12961 accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat
    13021 tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta
    13081 gtagt
//
