LOCUS       sequence-410222-        3060 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses LAUNCHERsbmv component in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: 
            pCMV-PDGFR-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-tTA
FEATURES             Location/Qualifiers
     source          1..3060
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     CDS             64..93
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             97..243
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             1201..1227
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1237..1263
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             join(2056..2673,2674..3060)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTIE
                     GLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHAD
                     ALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(2126..2145)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 atggagacag acacactcct gctatgggta ctgctgctct gggttccagg ttccactggt
       61 gacgaacaaa aactcatctc agaagaggat ctgaatgctg tgggccagga cacgcaggag
      121 gtcatcgtgg tgccacactc cttgcccttt aaggtggtgg tgatctcagc catcctggcc
      181 ctggtggtgc tcaccatcat ctcccttatc atcctcatca tgctttggca gaagaagcca
      241 cgtggtggct ctggaggact cgagaagagc ctgagggcca ccgtgagcga gagcagcatg
      301 atcctgcccg agggcaaggg cagcttctgg atccactgga tcaccaccca ggacggcttc
      361 tgcggcctgc ccctggtgag cgtgaacgac ggccacatcg tgggcatcca cggcctgacc
      421 agcaacgaca gcgagaagaa cttcttcgtg cccctgaccg acggcttcga gaaggagtac
      481 ctggagaacg ccgacaacct gagctgggac aagcactggt tctgggagcc cagcaagatc
      541 gcctggggca gcctgaacct ggtggaggag cagcccaagg aggagttcaa gatcagcaag
      601 ctggtgagcg acctgttcgg caacaccgtg accgtgcaga ccgcatgcgg tggcggtggc
      661 tctggaggtg gtgggtccgg aggaggaggt ggcggttccg gcggaggtgg gtctggtgga
      721 ggtggatctg gaggtggtgg atcaggagga ggaggttcag gcggccgcga gtttctggca
      781 accacactgg aacggatcga gaaaaatttc gtgattactg atccgagact gcctgacaac
      841 ccaatcattt ttgcgagcga ttccttcctg caactgacag aatattctcg ggaagagatc
      901 ctggggcgca attgccgttt tctgcaagga cccgagacag accgtgccac tgttcggaaa
      961 atcagagatg ctattgacaa ccagactgaa gtgaccgttc agctgatcaa ttataccaag
     1021 agcggcaaga agttctggaa cgtgttccac ctgcaaccga tgcgcgatta taagggcgac
     1081 gtccagtact tcattggcgt gcagctggat ggcaccgaac gtcttcatgg cgccgctgag
     1141 cgtgaggcgg tctgcctgat caaaaagaca gcctttcaga ttgctggatc catgcttcaa
     1201 cttcctcctc ttgaacgtct tactctcatg ttgcagctac caccattaga gagactaaca
     1261 ctgagtaaga gcgtgtacaa gggcctgagg gactacagcg gcatcagcac cctgatctgc
     1321 cagctgacca acagcagcga cggccacaag gagaccatgt tcggcgtggg ctacggcagc
     1381 ttcatcatca ccaacggcca cctgttcagg aggaacaacg gcatgctgac cgtgaagacc
     1441 tggcacggcg agttcgtgat ccacaacacc acccagctga agatccactt catccagggc
     1501 agggacgtga tcctgatcag gatgcccaag gacttccccc ccttcggcaa gaggaacctg
     1561 ttcaggcagc ccaagaggga ggagagggtg tgcatggtgg gcaccaactt ccaggaggaa
     1621 ttcctggcaa ccacactgga acggatcgag aaaaatttcg tgattactga tccgagactg
     1681 cctgacaacc caatcatttt tgcgagcgat tccttcctgc agctgacaga atattctcgg
     1741 gaagagatcc tggggcgcaa ttgccgtttt ctgcagggac ccgagacaga ccgtgccact
     1801 gttcggaaaa tcagagatgc tattgacaac cagactgaag tgaccgttca gctgatcaat
     1861 tataccaaga gcggcaagaa gttctggaac gtgttccacc tgcagccgat gcgcgattat
     1921 aagggcgacg tccagtactt cattggcgtg cagctggatg gcaccgaacg tcttcatggc
     1981 gccgctgagc gtgaggcggt ctgcctgatc aaaaagacag cctttcagat tgctgagagc
     2041 gtgagcctgc aaggttctag attagataaa agtaaagtga ttaacagcgc attagagctg
     2101 cttaatgagg tcggaatcga aggtttaaca acccgtaaac tcgcccagaa gctaggtgta
     2161 gagcagccta cattgtattg gcatgtaaaa aataagcggg ctttgctcga cgccttagcc
     2221 attgagatgt tagataggca ccatactcac ttttgccctt tagaagggga aagctggcaa
     2281 gattttttac gtaataacgc taaaagtttt agatgtgctt tactaagtca tcgcgatgga
     2341 gcaaaagtac atttaggtac acggcctaca gaaaaacagt atgaaactct cgaaaatcaa
     2401 ttagcctttt tatgccaaca aggtttttca ctagagaatg cattatatgc actcagcgct
     2461 gtggggcatt ttactttagg ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa
     2521 gaaagggaaa cacctactac tgatagtatg ccgccattat tacgacaagc tatcgaatta
     2581 tttgatcacc aaggtgcaga gccagccttc ttattcggcc ttgaattgat catatgcgga
     2641 ttagaaaaac aacttaaatg tgaaagtggg tccgcgtaca gccgcgcgcg tacgaaaaac
     2701 aattacgggt ctaccatcga gggcctgctc gatctcccgg acgacgacgc ccccgaagag
     2761 gcggggctgg cggctccgcg cctgtccttt ctccccgcgg gacacacgcg cagactgtcg
     2821 acggcccccc cgaccgatgt cagcctgggg gacgagctcc acttagacgg cgaggacgtg
     2881 gcgatggcgc atgccgacgc gctagacgat ttcgatctgg acatgttggg ggacggggat
     2941 tccccgggtc cgggatttac cccccacgac tccgccccct acggcgctct ggatatggcc
     3001 gacttcgagt ttgagcagat gtttaccgat gcccttggaa ttgacgagta cggtgggtag
//
