LOCUS       sequence-410247-        3222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses DRD2-V2tail-TevN-BLITz-TetR-FKBP component in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: DRD2-V2tail-TevN-BLITz-TetR-FKBP
FEATURES             Location/Qualifiers
     source          1..3222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1414..1440
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2254..2274
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             2275..2892
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="SRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHVK
                     NKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRP
                     TEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTTD
                     SMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(2345..2364)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     CDS             2899..3222
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
ORIGIN
        1 atgaagacga tcatcgccct gagctacatc ttctgcctgg tattcgccga ctacaaggac
       61 gatgatgacg ccagcatgga tccactgaac ctgtcctggt atgatgatga tctggagagg
      121 cagaactgga gccggccctt caatgggtca gaagggaagg cagacaggcc ccactacaac
      181 tactatgcca tgctgctcac cctcctcatc tttatcatcg tctttggcaa tgtgctggtg
      241 tgcatggctg tatcccgaga gaaggctttg cagaccacca ccaactactt gatagtcagc
      301 cttgctgtgg ctgatcttct ggtggccaca ctggtaatgc cgtgggttgt ctacctggag
      361 gtggtgggtg agtggaaatt cagcaggatt cactgtgaca tctttgtcac tctggatgtc
      421 atgatgtgca cagcaagcat cctgaacctg tgtgccatca gcattgacag gtacacagct
      481 gtggcaatgc ccatgctgta taacacacgc tacagctcca agcgccgagt tactgtcatg
      541 attgccattg tctgggtcct gtccttcacc atctcctgcc cactgctctt cggactcaac
      601 aatacagacc agaatgagtg tatcattgcc aaccctgcct ttgtggtcta ctcctccatt
      661 gtctcattct acgtgccctt catcgtcact ctgctggtct atatcaaaat ctacatcgtc
      721 ctccggaagc gccggaagcg ggtcaacacc aagcgcagca gtcgagcttt cagagccaac
      781 ctgaagacac cactcaagga tgctgcccgc cgagctcagg agctggaaat ggagatgctg
      841 tcaagcacca gccccccaga gaggacccgg tatagcccca tccctcccag tcaccaccag
      901 ctcactctcc ctgatccatc ccaccacggc ctacatagca accctgacag tcctgccaaa
      961 ccagagaaga atgggcacgc caagattgtc aatcccagga ttgccaagtt ctttgagatc
     1021 cagaccatgc ccaatggcaa aacccggacc tcccttaaga cgatgagccg cagaaagctc
     1081 tcccagcaga aggagaagaa agccactcag atgcttgcca ttgttctcgg tgtgttcatc
     1141 atctgctggc tgcccttctt catcacgcac atcctgaata tacactgtga ttgcaacatc
     1201 ccaccagtcc tctacagcgc cttcacatgg ctgggctatg tcaacagtgc cgtcaacccc
     1261 atcatctaca ccaccttcaa catcgagttc cgcaaggcct tcatgaagat cttgcactgc
     1321 gaattcggac gcaccccacc cagcctgggt ccccaagatg agtcctgcac caccgccagc
     1381 tcctccctgg ccaaggacac ttcatcgctt caacttcctc ctcttgaacg tcttactctc
     1441 ggtggctctg gaggtctcga gatgggcgag agcctgttca agggacctag ggactacaac
     1501 cctatcagta gcacaatttg tcacctgacc aacgagagtg atggccacac aacaagcctg
     1561 tacggcatcg ggttcggacc ctttatcatc accaacaagc acctgttcag gcggaataat
     1621 ggcactctgc tggtgcagag cctgcacggg gtgttcaaag tgaagaacac aaccactctg
     1681 cagcagcacc tgatcgatgg gcgggatatg atcatcatta ggatgcccaa ggacttcccc
     1741 ccttttcctc agaaactgaa gttccgagag ccccagagag aggagagaat ctgtctggtg
     1801 accacaaact ttcaggagct cggtagtggt agtggggagt ttctggcaac cacactggaa
     1861 cggatcgaga aaaatttcgt gattactgat ccgagactgc ctgacaaccc aatcattttt
     1921 gcgagcgatt ccttcctgca gctgacagaa tattctcggg aagagatcct ggggcgcaat
     1981 tgccgttttc tgcagggacc cgagacagac cgtgccactg ttcggaaaat cagagatgct
     2041 attgacaacc agactgaagt gaccgttcag ctgatcaatt ataccaagag cggcaagaag
     2101 ttctggaacg tgttccacct gcagccgatg cgcgattata agggcgacgt ccagtacttc
     2161 attggcgtgc agctggatgg caccgaacgt cttcatggcg ccgctgagcg tgaggcggtc
     2221 tgcctgatca aaaagacagc ctttcagatt gctgagaacc tgtacttcca gggctctaga
     2281 ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa
     2341 ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg
     2401 catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac
     2461 catactcact tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct
     2521 aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca
     2581 cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa
     2641 ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt
     2701 tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact
     2761 gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag
     2821 ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt
     2881 gaaagtgggt ccaccggtgg agtgcaggtg gaaaccatct ccccaggaga cgggcgcacc
     2941 ttccccaagc gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag
     3001 aaatttgatt cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag
     3061 gtgatccgag gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg
     3121 actatatctc cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat
     3181 gccactctcg tcttcgatgt ggagcttcta aaactggaat ag
//
