LOCUS       sequence-410249-         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses NLS-FRB-VP16 component in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: pCMV-NLS-FRB-VP16
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..24
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             46..324
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             481..714
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 atgcctaaaa agaagcgtaa agtcggagga tccatggctt ctcgaatcct ctggcatgag
       61 atgtggcatg aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa
      121 ggcatgtttg aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg
      181 aaggaaacat cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc
      241 aggaagtaca tgaaatcagg gaatgtcaag gacctcctcc aagcctggga cctctattat
      301 catgtgttcc gacgaatctc aaagactagt gcgtacagcc gcgcgcgtac gaaaaacaat
      361 tacgggtcta ccatcgaggg cctgctcgat ctcccggacg acgacgcccc cgaagaggcg
      421 gggctggcgg ctccgcgcct gtcctttctc cccgcgggac acacgcgcag actatcgacg
      481 gcccccccga ccgatgtcag cctgggggac gagctccact tagacggcga ggacgtggcg
      541 atggcgcatg ccgacgcgct agacgatttc gatctggaca tgttggggga cggggattcc
      601 ccgggtccgg gatttacccc ccacgactcc gccccctacg gcgctctgga tatggccgac
      661 ttcgagtttg agcagatgtt taccgatgcc cttggaattg acgagtacgg tgggtag
//
