LOCUS       sequence-410251-        4179 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses 
            NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq
            --bArr2-TevCs component in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Cui M, Lee S, Ban SH, Ryu JR, Shen M, Yang SH, Kim JY, Choi SK, Han 
            J, Kim Y, Han K, Lee D, Sun W, Kwon HB, Lee D
  TITLE     A single-component, light-assisted uncaging switch for 
            endoproteolytic release.
  JOURNAL   Nat Chem Biol. 2024 Mar;20(3):353-364. doi: 
            10.1038/s41589-023-01480-6. Epub 2023 Nov 16.
  PUBMED    37973890
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-023-01480-6"; journalName: "Nat Chem Biol"; date: 
            "2024-03"; volume: "20"; issue: "3"; pages: "353-364"
COMMENT     Sequence Label: 
            pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr
            2-TevCs
FEATURES             Location/Qualifiers
     source          1..4179
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(130..132,133..843)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(130..132,133..843)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(172..193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(433..452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     780..801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1726..1752
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1762..1788
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             2563..2583
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 atggtgggcc gcaacagcgc gattgcagct ggagtgtgcg gcgcgctgtt cattggctat
       61 tgcatttact ttgatcgcaa acgccgcagc gatccgaact ttaaaggcgg tggagggtcc
      121 tctagagggg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
      181 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
      241 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
      301 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
      361 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
      421 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
      481 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
      541 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
      601 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
      661 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
      721 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
      781 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
      841 aagggttccg gaagcggaga attcaagtcc atgtccagca tggtgagcga tactagctgt
      901 accttcccat catctgacgg aatcttctgg aagcactgga ttcagactaa ggacggccag
      961 tgtggcagcc cactggtgag cacacgagac ggattcatcg tggggattca cagcgcctcc
     1021 aactttacaa acaccaataa ctatttcacc tcagtgccaa agaactttat ggagctgctg
     1081 accaaccagg aggcccagca gtgggtgagc gggtggcgcc tgaacgccga ttccgtgctg
     1141 tggggcgggc acaaggtgtt tatgaccgca tgcggtggcg gtggctctgg aggtggtggg
     1201 tccggaggag gaggtggcgg ttccggcgga ggtgggtctg gtggaggtgg atctggaggt
     1261 ggtggatcag gaggaggagg ttcaggcggc cgcgagtttc tggcaaccac actggaacgg
     1321 atcgagaaaa atttcgtgat tactgatccg agactgcctg acaacccaat catttttgcg
     1381 agcgattcct tcctgcaact gacagaatat tctcgggaag agatcctggg gcgcaattgc
     1441 cgttttctgc aaggacccga gacagaccgt gccactgttc ggaaaatcag agatgctatt
     1501 gacaaccaga ctgaagtgac cgttcagctg atcaattata ccaagagcgg caagaagttc
     1561 tggaacgtgt tccacctgca accgatgcgc gattataagg gcgacgtcca gtacttcatt
     1621 ggcgtgcagc tggatggcac cgaacgtctt catggcgccg ctgagcgtga ggcggtctgc
     1681 ctgatcaaaa agacagcctt tcagattgct ggatccatgc ttcaacttcc tcctcttgaa
     1741 cgtcttactc tcatgttgca gctaccacca ttagagagac taacactgat gggcgagagc
     1801 ctgttcaagg gacctaggga ctacaaccct atcagtagca caatttgtca cctgaccaac
     1861 gagagtgatg gccacacaac aagcctgtac ggcatcgggt tcggaccctt tatcatcacc
     1921 aacaagcacc tgttcaggcg gaataatggc actctgctgg tgcagagcct gcacggggtg
     1981 ttcaaagtga agaacacaac cactctgcag cagcacctga tcgatgggcg ggatatgatc
     2041 atcattagga tgcccaagga cttcccccct tttcctcaga aactgaagtt ccgagagccc
     2101 cagagagagg agagaatctg tctggtgacc acaaactttc agaccgagtt tctggcaacc
     2161 acactggaac ggatcgagaa aaatttcgtg attactgatc cgagactgcc tgacaaccca
     2221 atcatttttg cgagcgattc cttcctgcag ctgacagaat attctcggga agagatcctg
     2281 gggcgcaatt gccgttttct gcagggaccc gagacagacc gtgccactgt tcggaaaatc
     2341 agagatgcta ttgacaacca gactgaagtg accgttcagc tgatcaatta taccaagagc
     2401 ggcaagaagt tctggaacgt gttccacctg cagccgatgc gcgattataa gggcgacgtc
     2461 cagtacttca ttggcgtgca gctggatggc accgaacgtc ttcatggcgc cgctgagcgt
     2521 gaggcggtct gcctgatcaa aaagacagcc tttcagattg ctgagaacct gtacttccag
     2581 ggcaccggta tgggtgaaaa acccgggacc agggtcttca agaagtcgag ccctaactgc
     2641 aagctcaccg tgtacttggg caagcgtgac tttgtggatc acttggacaa agtggatcct
     2701 gtcgatggtg tggtgcttgt ggatcctgac tacttgaagg accggaaagt gtttgtgacc
     2761 ctcacctgtg ccttccgcta tggccgagaa gacctggatg tactgggcct gtctttccgc
     2821 aaagatctgt tcatcgccac ctaccaggcc ttccccccca tgcccaaccc acctcggccc
     2881 cccacccgcc tacaggaccg actgctgaag aagttgggcc agcacgccca cccctttttt
     2941 ttcacaatac cccagaattt gccttgctcc gtcacactgc agccaggacc ggaggacaca
     3001 gggaaggcct gtggagtaga ctttgagatt cgagccttct gtgccaaatc tatagaagaa
     3061 aaaagccaca aaaggaactc cgtgcggctt atcatcagaa aggtacagtt tgctcctgag
     3121 acacccggcc cccagccatc agctgaaacc acacgccact tcctcatgtc tgaccggagg
     3181 tccctgcacc tagaggcttc cctggacaaa gagctgtact accatgggga acccctcaat
     3241 gtcaacgtcc acgtcaccaa caattctgcc aaaaccgtca agaagatcag agtgtctgtg
     3301 agacagtatg ccgacatttg cctcttcagc accgcgcagt acaagtgtcc tgtggctcag
     3361 cttgaacaag atgaccaggt gtctcccagt tccacattct gcaaggtgta caccataacc
     3421 ccgctgctca gtgacaaccg agagaagcgt ggccttgccc ttgatgggca actcaagcac
     3481 gaagacacca acctggcttc cagcaccatt gtgaaggagg gagccaacaa ggaggtgctg
     3541 ggaatcctag tatcctacag ggtcaaggtg aagctggtgg tgtctcgagg cggggatgtc
     3601 tccgtggagc tacctttcgt cctaatgcac cccaagcccc acgaccacat cacccttccc
     3661 cgaccccagt cagccccccg ggaaatagac atccctgtgg ataccaacct cattgaattc
     3721 gataccaact atgccacaga cgacgacatc gtgtttgagg actttgcgag gcttcggctg
     3781 aaggggatga aggatgacga ctgtgatgac cagttctgcc tgagctcact cgaaggtggc
     3841 ggtggctctg gaggtggtgg gtccggagga ggcggcatcg agatgtccag catggtgagc
     3901 gatactagct gtaccttccc atcatctgac ggaatcttct ggaagcactg gattcagact
     3961 aaggacggcc agtgtggcag cccactggtg agcacacgag acggattcat cgtggggatt
     4021 cacagcgcct ccaactttac aaacaccaat aactatttca cctcagtgcc aaagaacttt
     4081 atggagctgc tgaccaacca ggaggcccag cagtgggtga gcgggtggcg cctgaacgcc
     4141 gattccgtgc tgtggggcgg gcacaaggtg tttatgtag
//
