LOCUS       sequence-410269-       12600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rabbit Cav2.2 calcium channel with an exofacial double
            HA tag in domain II and an E1658K mutation in the P loop of Domain 
            IV which abolishes trafficking to the cell surface.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12600)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.2 HA EIVK (E1658K) pcDNA3
FEATURES             Location/Qualifiers
     source          1..12600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      168..303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     230..249
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             384..1178
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(438..457)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1048..1067
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1352..1473
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1389..1408)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1443..1462
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1522..1538)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1522..1538)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1535..1557)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1546..1562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1570..1576,1577..1594,1595..1600))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1615..1636
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1753..1770)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1924..2512)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2004..2023)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2683..3474,3475..3543))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3306..3325
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3544..3648)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3723..3742)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3914..4293
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4294..4497
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4448..4468
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4542..4561
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4542..4560
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6362..6388
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6392..6418
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        complement(11832..11850)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11833..11850)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11870..11887)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11876..12100
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12146..12574
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12233..12252)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12443..12464
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(join(12583..12600,1..3))
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12588..12600,1..317)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
       61 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
      181 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
      241 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
      301 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
      361 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
      421 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
      481 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
      541 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
      601 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
      661 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
      721 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
      781 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
      841 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
      901 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
      961 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     1021 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     1081 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     1141 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     1201 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     1261 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     1321 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     1381 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     1441 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     1501 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     1561 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     1621 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     1681 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     1741 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     1801 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     1861 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     1921 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     1981 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2041 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2101 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2161 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2221 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2281 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2341 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     2401 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     2461 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     2521 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     2581 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     2641 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     2701 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     2761 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     2821 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     2881 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     2941 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     3001 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     3061 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     3121 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     3181 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     3241 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     3301 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     3361 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     3421 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     3481 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     3541 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     3601 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     3661 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     3721 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     3781 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     3841 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     3901 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     3961 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     4021 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     4081 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     4141 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     4201 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     4261 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     4321 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     4381 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     4441 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     4501 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     4561 gagacccaag cttggtaccg agctcggatc cactagtaac ggccgccagt gtgctggaat
     4621 tcctcgaggg taccgggccc cccctcgagg tcgaagcttg atcatggtcc gcttcgggga
     4681 cgagctgggc ggccgctatg ggggcgcggg cggcgcggag cgggcccggg gcggcggggc
     4741 cggcggggct ggcggcccgg gccccggggg gctgccgccg ggccagcggg tcctctacaa
     4801 acagtcgatc gcgcagcgcg cgcggaccat ggcgctgtac aaccccatcc cggtcaagca
     4861 gaactgcttc accgtcaacc gctcgctctt cgtcttcagc gaggacaacg tcgtccgcaa
     4921 atacgccaag cgcatcaccg agtggcctcc gttcgagtac atgatcctgg ccaccatcat
     4981 cgccaactgc atcgtgctgg ccctggagca gcacctccct gacggggaca agacgcctat
     5041 gtccgagcgc ctggatgaca ccgagcccta cttcattggg atcttctgct tcgaggcggg
     5101 gatcaagatc ctggccctgg gctttgttct gcacaagggc tcctatctgc ggaacggctg
     5161 gaacgtcatg gacttcgtgg tagtcctcac ggggattctt gccacagccg gaaccgactt
     5221 cgacctgcga acgctgaggg ctgtgcgtgt gctaaggccc ctgaagctgg tgtctgggat
     5281 tccgagcttg caagtggtgc tcaagtccat catgaaggcc atggtcccgc tgctgcagat
     5341 cgggctgctg ctcttcttcg ccatcctcat gtttgccatc atcggcctcg agttctacat
     5401 gggcaagttc cacaaggcct gcttccccaa cagcacagac ccagatcccg tgggtgactt
     5461 cccctgtggc aaggaggccc cggcccggct ctgtgaggga gacacggagt gccgggagta
     5521 ctgggccgga cccaactttg gtatcaccaa ctttgacaac atcctgtttg ccatcttgac
     5581 cgtgttccag tgcatcacca tggagggctg gactgacatc ctctacaata caaacgatgc
     5641 agccggcaac acgtggaact ggctctactt catccctctc atcatcatcg gctccttctt
     5701 catgctcaac ctggtgctgg gtgtgctctc aggagagttt gctaaggagc gcgagagagt
     5761 ggagaaccgc cgcgccttcc tgaagctccg gcggcagcag cagattgagc gggagctcaa
     5821 cgggtacctg gagtggatct tcaaggctga ggaagtcatg ctggctgagg aggacaggaa
     5881 cgcagaggag aagtcccctc tggatgcagt gttgaagaga gcagctgcca agaagagcag
     5941 aagcgacctg atccaggccg aggaggggga gggccggctc acaggcctct gtgctcccgg
     6001 ttccccgttc gcccgtgcca gcctgaaaag cgggaagacg gagagctcgt cgtacttccg
     6061 gaggaaggag aagatgttcc gcttcttcat ccggcgcatg gtgaaggcac agagcttcta
     6121 ctggacggtg ctgtgtgtgg tggcactgaa cacactgtgc gtggccatgg tgcactacaa
     6181 ccagccgcag cggctcacca cggcattgta ctttgcagag tttgttttcc tgggtctctt
     6241 cctcacagag atgtccctga agatgtatgg cctggggccc aggagctact tccggtcctc
     6301 cttcaactgt tttgactttg gggtcatcgt gggcagtatc tttgaagtgg tctgggctgc
     6361 ttacccctac gacgtccccg actacgccgg ctacccgtac gacgtcccgg actacgccgg
     6421 cgtcgacgtc aagccaggga cctccttcgg gatcagtgtt ctgcgggctc tgcgcttact
     6481 gagaatcttc aaggtcacca aatactggaa ctccctacgg aacctggtgg tgtctctgct
     6541 caactccatg aagtccatca tcagcctcct cttcctgctc ttcctgttca ttgttgtctt
     6601 tgctctgctg gggatgcagc tgtttggagg ccagttcaac tttaaagacg agaccccaac
     6661 gaccaacttt gacaccttcc ctgctgctat cctcaccgtc ttccagatcc tgaccggaga
     6721 ggactggaat gcggtcatgt accacgggat cgagtcgcaa ggtggcgtca gcagaggaat
     6781 gttctcctcc ttctacttca ttgtcctcac tctgtttggg aactacaccc tgctgaatgt
     6841 cttcctggcc attgctgtgg acaaccttgc caatgctcag gagctgacca aggatgaaga
     6901 ggagatggaa gaagcagcca atcagaaact cgccttgcag aaggccaagg aagtggctga
     6961 agtcagcccc atgtctgctg cgaacatctc cattgccgcc aggcagcaga actcggccaa
     7021 agcgcgctcg gtgtgggagc agcgagccag ccagctgcgg ctgcagaacc tgagggccag
     7081 ctgcgaggcg ctgtacagcg agatggaccc cgaggagagg ctgcgctacg ccaccgcgcg
     7141 ccacctgcgg cccgacgtga agacgcacct ggaccggccg ctggtggttg agcccggccg
     7201 cgacgctccg cggggccccc cgggggggaa gtcccggccg gatggctcgg aggcccccga
     7261 gggtgcggat cccccgcgca ggcatcaccg gcaccgagac aaggacaagg ctcccgccac
     7321 ggtcccctcg gcgggggaac aggacagagc cgaagctctg agggcagagg gcggggagct
     7381 ggggccccgc gaggagcggg ggcggccacg ccgcagccgc agcaaggagg ccccgggcgc
     7441 cccggaggtg cgcagcgatc gcggccgtgg cccgtgtcct gaaggtggtc ggcgccacca
     7501 ccggcgcggg tcaccggagg aggcagctga gagggagccc cgccgccacc gcgcgcaccg
     7561 gcacgggccc gacccgggca aggagggccc agcgtccggg accaggggcg agcggcgcgc
     7621 acgacaccgc acgggccccc gggcgtgccc ccgggaggct gagagcagcg aggagcctgc
     7681 gcggcggcac cgggctcggc acaaggcgcc gcccacccag gagaccgcgg agaaggacaa
     7741 ggaggctgcc gagaaggggg gcgaggccac ggaggccgag aaggacaagg aggctcggaa
     7801 ccaccagccc aaggagctgc cctgtgacct ggaggccatc ggcatgctgg gagtgggcgc
     7861 tgtgcatacg ctgcccagca cctgtctgca gaaggtggag gagcagccag aggatgcgga
     7921 caaccagcgg aacgtcaccc gcatgggtag tcagcccccg gacaccagca ccactgtgca
     7981 cattcctgtg acgctgaccg gcccccccgg ggagaccacg gtagtgccca gtggaaacgt
     8041 ggacctggaa agtcaagcag aggggaagaa ggaggtggaa accagcgatg tgatgaggag
     8101 cggccctcgg cccattgtcc cgtacagttc catgttctgt ttaagcccaa ccaacctgct
     8161 gcgccgctgc tgccactaca ttgtgaccat gcgatacttc gagatggtca tcctcgtggt
     8221 catcgccctg agcagcatcg ccctggctgc cgaggacccc gtgagaacag actctcccag
     8281 gaataatgct ctgaagtaca tggactacat cttcacaggc gtcttcacct tcgagatggt
     8341 gataaagatg atcgatttgg gactgctgct acaccctgga gcctatttcc gggatctgtg
     8401 gaacattctg gacttcattg tggtcagtgg ggccctggtg gcatttgctt tctcaggatc
     8461 caaagggaaa gacatcagca ccatcaaatc cctgagagtc ctgcgtgtgc tgcggccact
     8521 caagaccatc aagcggctgc ccaagctcaa ggccgtgttt gactgtgtgg tgaactcact
     8581 gaaaaacgtc ctcaacatcc tgatcgtcta catgctcttc atgttcatct ttgccgtcat
     8641 tgccgtgcag ctcttcaaag ggaagttctt ctactgcacg gacgaatcca aggagctgga
     8701 gcgtgactgc aggggtcagt atttggatta tgagaaggag gaagtggaag ctcaaccgag
     8761 gcagtggaag aaatatgact tccactatga caacgtgctc tgggctctgc taacgctctt
     8821 cacggtgtcc acaggggaag ggtggcccat ggtgctgaaa cactctgtgg atgccaccta
     8881 cgaggagcag ggcccgagcc ccgggtaccg catggagctc tccatcttct acgtggtcta
     8941 ctttgtggtc tttcccttct tctttgtcaa catcttcgtg gccttgatca tcatcacctt
     9001 ccaggagcag ggggacaagg tgatgtccga gtgcagcctg gagaagaacg agagggcttg
     9061 catcgacttt gccatcagcg ccagacccct gacgcggtac atgccccaga acaagcagtc
     9121 gttccagtat aagacgtgga cgttcgtggt gtccccacct tttgagtact tcatcatggc
     9181 tatgatcgcc ctcaacactg tggtgctgat gatgaagttc tacgatgcgc cgtacgagta
     9241 tgagctaatg ctgaaatgcc tgaacatcgt gttcacgtcc atgttctcca tggagtgtgt
     9301 gctgaagatc atcgccttcg gggtgctgaa ctatttcaga gatgcctgga atgtctttga
     9361 ctttgtcacg gtgttgggaa gtattactga tattttggta acagagattg cgaacaactt
     9421 catcaacctc agcttcctgc gcctgttccg ggccgcgcgg ctcatcaagc tgctgcgcca
     9481 gggctacacc atccgcatcc tgctttggac cttcgtgcag tccttcaagg cgctgcccta
     9541 cgtgtgtctc ctcatcgcca tgctcttctt catctacgcc atcattggca tgcaggtttt
     9601 tgggaacatc gccctggacg atgacaccag catcaacagg cacaataact tccggacgtt
     9661 tctgcaggcc ttgatgttgc tgttcaggag cgccacgggg aaggcctggc acgagataat
     9721 gctgtcgtgc ctcagcagcc gggcctgcga cgagcactcc aatgccagcg agtgcggcag
     9781 cgactttgcc tacttttact tcgtctcctt catcttcctc tgctcctttc tgatgttgaa
     9841 cctctttgtg gctgtgatca tggacaattt tgagtacctt acacgggact cttccatctt
     9901 agggcctcac cacctggatg agttcatccg ggtctgggct gaatacgacc cggccgcgtg
     9961 tgggcgcatc agttacagtg acatgtttga gatgctgaag cacatgtccc cacccctggg
    10021 gctggggaag aagtgccctg ctcgagtcgc gtacaagcgc ctggtccgca tgaacatgcc
    10081 catctccagt gaggacatga cggtgcactt cacatccacg ctgatggctc tcattcggac
    10141 agcgctggac atcaagctgg caccagctgg gaccaagcag caccagtgcg acgcagagct
    10201 gaggaaggag atttcctgtg tgtgggccaa tctgccccag aagaccctgg acttactggt
    10261 accaccccat aagcctgacg agatgacggt gggcaaagtc tatgcagctc tgatgatctt
    10321 cgacttctac aaacagaaca aaaccagcag agaccagact cagcaagccc ccggaggcct
    10381 gtcccagctg ggccccgtgt ccctgttcca cccgctgaag gccacgctgg agcagactca
    10441 gccggcgctg cgtggagccc gggctttcct gcggcagaag agttctgcct ccctgagcaa
    10501 cggcggggcc gtacaaaccc aggagagcgg catcaaggaa tcggtttctt ggggcactca
    10561 gaggacccag gatgtgcttt gcgaggccag ggcacccctg gagcggggcc actcagctga
    10621 gatcccagtc gggcagcctg ggacactggc tgtagatgtc cagatgcaga acatgactct
    10681 gagtggccct gacgccgagc cgcagccagg gctggagagc cagggccgag ccgcctccat
    10741 gccccgcctg gccgcagaaa cacagcccgc gcctgacgcc agccccatga agcgctccat
    10801 ctccacgctg gccccgcgcc cccacacggc ccggctcggc agcaccgcgc tggaccgccc
    10861 agcccccagc caggccccgc accatcacca ccaccgctgc caccgccgca gggacaggaa
    10921 gcagcggtcc ctggagaagg gtcccagcct gtctgctgac acagatgggg cccccgacag
    10981 cacggtgggg ccaggactgc ccacggggga gggccccccc ggctgcaggc gggagcggga
    11041 gcgcaggcag gagcggggcc ggtcccagga gcggaggcag ccctcgtcct cctcctcgga
    11101 gaagcatcgc ttctactcct gtgaccgctt cgggggccgc gaacccccac agcccaagcc
    11161 ctccctcagt agtcacccca cgtcgccgac ggccgggcag gagccgggac cccacccgca
    11221 ggggagtggt tcggttcacg ggagccccct gctgtccaca tctggtgcta gcaccccggg
    11281 ccgggggcgg aggcagctcc cccagacccc cttgaccccc cgccccagcg tcacctacaa
    11341 gacggccaac tcctcgcctg tccacttcgc cggggcaccg agcggcctcc ccgccttctc
    11401 ccccggccgg ctcagccggg gcctctcaga acacaacgcc ctgctgcaga gggacccgct
    11461 cagccggccc ctggcgcccg gctcgcgcat cggctccgac ccctacctgg ggcagcggct
    11521 ggacagcgag gcgcccgccc gtgccctgcc cgaggacgcg cccgcctttg aggagaccgc
    11581 ggccagcaac tcgggccgct cctcccggac ctcgtacgtg tcctcactga cctcgcagcc
    11641 cccgccgctg cgccgcgtgc ccaacggcta ccactgcact ctggggctgg gcgggggcgg
    11701 ccgggcgcgg cgcggctgcc accaccccga ccgcgaccgc cggtgctaga ctagttctag
    11761 caagcttcga cgaattctgc agatatccat cacactggcg gccgctcgag catgcatcta
    11821 gagggcccta ttctatagtg tcacctaaat gctagagctc gctgatcagc ctcgactgtg
    11881 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    11941 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    12001 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    12061 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    12121 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    12181 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    12241 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    12301 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
    12361 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
    12421 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
    12481 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
    12541 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
//
