LOCUS       sequence-410287-       12992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 calcium channel with a E1686R mutation to 
            enhance expression and a mutation in the P loop of Domains I, II, 
            III and IV to to abolish divalent cation binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.1 HA EI,II,III,IVA (E320A, E670A, E1411A,...
FEATURES             Location/Qualifiers
     source          1..12992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     47..66
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             201..995
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(255..274)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     865..884
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1169..1290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1206..1225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1260..1279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1339..1355)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1339..1355)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1352..1374)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1363..1379
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1387..1393,1394..1411,1412..1417))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1432..1453
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1570..1587)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1741..2329)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1821..1840)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2500..3291,3292..3360))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3123..3142
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3361..3465)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3540..3559)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3731..4110
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4111..4314
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4265..4285
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4359..4378
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4359..4377
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6199..6225
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6229..6255
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             11032..11061
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(12041..12059)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12042..12059)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(12079..12096)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12085..12309
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12355..12783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12442..12461)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12652..12673
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12792..12812)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12797..12992,1..134)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12977..12992,1..120)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
       61 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
      121 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga
      181 caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg
      241 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      301 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      361 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
      421 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      481 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      541 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      601 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
      661 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
      721 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
      781 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
      841 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
      901 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
      961 tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt tcgaaatgac
     1021 cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg ccttctatga
     1081 aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga
     1141 tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa
     1201 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1261 tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta
     1321 gagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1381 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1441 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1501 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1561 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1621 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1681 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1741 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1801 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1861 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1921 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1981 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     2041 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     2101 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2161 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2221 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2281 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2341 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2401 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2461 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2521 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2581 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2641 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2701 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2761 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2821 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2881 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2941 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     3001 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     3061 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3121 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3181 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3241 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3301 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3361 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3421 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3481 agtgccacct gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt
     3541 acaatctgct ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag
     3601 gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat
     3661 tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga
     3721 tatacgcgtt gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta
     3781 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     3841 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     3901 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     3961 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4021 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4081 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4141 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4201 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4261 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg
     4321 ctaactagag aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag
     4381 acccaagctt ggtaccgagc tcggatccac tagtaacggc cgccagtgtg ctggaattct
     4441 gcagatatcc atcacactgg cggccgctcg aggtcttgca gaatggcccg ctttggagac
     4501 gagatgccgg gccgctacgg cgcaggcgga ggaggctcag ggccggccgc cggggtggtc
     4561 gtgggcgccg cgggcggccg aggagccggg ggcagccggc agggcgggca gcccggagcg
     4621 cagaggatgt acaagcagtc gatggcgcag agagcgcgga ccatggccct ctacaacccc
     4681 atccctgtcc gccagaactg cctcacggtc aaccgctccc tgttcctctt cagtgaagac
     4741 aacgtggtga gaaaatacgc caaaaagatc acggaatggc ctcccttcga gtacatgatc
     4801 ctggccacca tcattgctaa ctgcatcgtc ctggccctgg agcagcacct ccctgatgat
     4861 gacaagacac ccatgtccga gcggctggat gacacagaac cctatttcat tggcatcttc
     4921 tgttttgagg ctggaattaa gatcgtggct cttggctttg ccttccacaa aggctcctac
     4981 ctgaggaatg gctggaacgt catggacttt gtcgtggtgc taacaggcat cttggccact
     5041 gtcgggacgg agtttgatct acggacactg agggcggttc gtgtgctgcg gccactcaag
     5101 ctggtgtctg gaatcccaag tttacaagtc gtcctgaagt caatcatgaa ggcgatgatc
     5161 cctctgctgc agatcggcct cctcctgttt tttgcaatcc ttatttttgc aatcataggg
     5221 ttagaatttt atatgggaaa atttcatacc acctgctttg aagaggggac agacgacatc
     5281 cagggtgagt cgccagctcc gtgtgggaca gaggagcctg cccgcacctg ccccaacggg
     5341 accaaatgtc agccgtactg ggaagggccc aacaacggca tcactcagtt cgacaacatc
     5401 ctgtttgctg tgctcactgt tttccagtgc atcaccatgg caggctggac tgatctcctc
     5461 tacaatagca acgatgcctc agggaacact tggaactggt tgtacttcat ccccctcatc
     5521 atcatcggct ccttttttat gctgaacctt gtgctgggtg tgctgtctgg ggagtttgcc
     5581 aaagaaaggg aacgtgtaga gaaccgaagg gcttttctga agctcagaag acaacagcag
     5641 attgaacgtg agctcaatgg atacatggag tggatctcga aagcagaaga ggtgatcctc
     5701 gcggaggacg aaacagacgt ggagcagagg cacccttttg atggagctct tcggagagct
     5761 actctgaaga aaagcaagac ggacctgctc aaccctgagg aggcggagga ccagcttgct
     5821 gacatcgcgt ctgtggggtc tcccttcgcc agagccagca tcaaaagtgc caagctggag
     5881 aattcgactt ttttccacaa aaaggagaga agaatgcgtt tctacatccg ccgcatggtc
     5941 aaaactcagg ccttctactg gaccgtgctc agtctggtag ccctcaacac gctgtgcgtc
     6001 gccattgtcc actacaacca gcccgagtgg ctctccgact tcctctacta tgcagaattc
     6061 attttcttag gactctttat gtccgaaatg tttataaaaa tgtatgggct cgggacacgg
     6121 ccttacttcc actcttcctt caactgcttt gactgtgggg tcatcatcgg gagcatcttt
     6181 gaagtcatct gggccgtcta cccctacgac gtccccgact acgccggcta cccgtacgac
     6241 gtcccggact acgccggcgt cgacatcaaa ccgggtacat cctttggaat cagcgtgtta
     6301 cgagctctca ggttactgcg tattttcaaa gtcacaaagt actgggcatc tctcagaaac
     6361 ctggttgtct ccctcctcaa ctccatgaaa tccatcataa gtctgctgtt cctcctcttc
     6421 ctcttcattg tcgtctttgc cctcttgggg atgcagctgt ttggtggcca gtttaatttt
     6481 gacgagggga ctcctcccac caacttcgac acttttccag cagcaataat gactgtgttt
     6541 cagatcctga ctggcgcgga ttggaatgag gtcatgtatg atgggatcaa gtctcagggg
     6601 ggcgtgcagg gcggcatggt gttctccatc tacttcatcg tcctcaccct cttcgggaac
     6661 tacaccctgc tgaacgtgtt cttggctatc gcggtggaca acctggccaa cgcccaggaa
     6721 ctcaccaagg atgaacaaga agaggaagag gcagccaatc agaaactggc tctacagaaa
     6781 gccaaggagg tggcagaagt gagtcccctg tctgcagcca acatgtccat agctgtgaag
     6841 gaacagcaga agaaccagaa gcctgccaag tcggtgtggg agcagcgcac cagcgagatg
     6901 cgcaagcaga acctgctggc tagccgcgag gcgctgtacg gggacgcggc tgagcgctgg
     6961 cccaccactt acgcgcgccc gctgcggccg gacgtgaaga cgcacttgga ccggccgctc
     7021 gtggtggacc cgcaggagaa ccgtaacaac aacaccaaca agagccgtgc gccagaagcg
     7081 ctgcgccaaa ccgcgcggcc ccgcgagagc gcgcgcgacc ccgacgcgcg gcgcgcctgg
     7141 cccagcagcc ctgagcgcgc ccctggacga gagggcccgt atggccgcga gagcgagccg
     7201 caacagcgcg agcacgcgcc accccgcgag cacgtaccct gggacgcgga tcctgagcgc
     7261 gccaaggccg gggacgcgcc ccgccgccac acgcaccggc ctgtggccga gggcgagcct
     7321 cgtcgccacc gcgcgcgccg ccggcccggg gacgaaccgg acgacagacc ggagcgcagg
     7381 ccgcgtcccc gcgacgccac taggccggcc cgcgctgcag acggcgaagg cgatgatggg
     7441 gagcgcaagc ggcgacaccg acacgggccg ccggcccacg atgacaggga gcgcagacac
     7501 cggcgcagaa aagagagcca gggctctggg gtccccatgt ctggtcccaa cctgtccacc
     7561 accaggccaa tccagcagga tctgggccgc caggacctgc cactggctga ggacctggac
     7621 aacatgaaga acaacaagtt ggccaccggg gagcctgcca gtccccacga cagcctgggc
     7681 cacagtggcc ttccccctag ccctgccaag atcgggaaca gcaccaaccc tggtcccgcc
     7741 ttggccacca atccccagaa tgctgccagc cgcaggacgc ccaacaaccc gggcaacccg
     7801 tccaaccccg gcccccccaa gactcccgag aacagcctta tcgtcaccaa ccccagcagc
     7861 acccagccca actcagcaaa gactgccagg aaacccgagc acatggcggt ggagatcccc
     7921 ccggcctgcc cgcccctcaa ccacactgtg gtccaagtaa acaaaaacgc caacccagac
     7981 ccactgccaa agaaagagga agagaagaag gaggaagagg aggcagaccc cggggaggat
     8041 ggcccaaagc ccatgccgcc ctacagctcc atgttcatcc tctccaccac caaccccctt
     8101 cgccggctgt gccattacat cctgaacctg cgctacttcg agatgtgcat cctcatggtc
     8161 attgccatga gtagcatcgc gctggccgcc gaggacccgg tgcagcccaa cgcaccccgc
     8221 aacaacgtgc tgcgatattt tgactatgtt ttcacaggag tgtttacctt tgagatggtg
     8281 atcaagatga tcgacctggg cctcgtcctg catcaggggg cctatttccg tgacctgtgg
     8341 aacattctgg acttcatagt ggtcagtggg gccctggtgg cctttgcctt cactggcaat
     8401 agcaaaggaa aggacatcaa caccatcaag tccctccgag tcctccgggt gctacgacct
     8461 ctaaagacca tcaagcggct gcctaagttg aaggccgtat ttgactgcgt ggtgaactcg
     8521 ctcaagaacg tcttcaacat cctcattgtc tacatgctct tcatgttcat cttcgccgtg
     8581 gtggccgtgc agctcttcaa gggcaaattc ttccactgca cggacgagtc caaggagttt
     8641 gagagagact gtcgaggcaa atacctcctt tacgagaaga acgaggtaaa ggcgcgggac
     8701 cgcgagtgga agaaatacga cttccactac gacaacgtgc tctgggccct gctcacgctc
     8761 tttacggtgt ccacgggagc gggctggcca caggtcctca agcactcagt ggatgccact
     8821 tttgagaacc agggccccag ccccgggtac cgcatggaaa tgtccatctt ctacgtggtc
     8881 tactttgtgg tgtttccctt cttctttgtc aatatctttg tggccttgat catcatcacc
     8941 ttccaggagc agggagacaa gatgatggaa gaatacagcc tagagaaaaa tgagagggcc
     9001 tgcatcgact ttgccatcag tgccaagccg ctgaccaggc acatgcccca gaacaagcag
     9061 agcttccagt atcgaatgtg gcagttcgtg gtgtccccac cctttgagta caccatcatg
     9121 gccatgatcg ctctcaacac catcgtgcta atgatgaagt tctatggagc ctctgtggcc
     9181 tatgaaaacg cccttcgagt gttcaacatt gtcttcacct ccctcttctc tctcgaatgt
     9241 gtgctcaaag tcatggcttt tgggattctg aattatttcc gcgatgcctg gaacatcttc
     9301 gactttgtga ctgttctggg cagcatcaca gacatcctcg tcaccgagtt tgggaataac
     9361 ttcatcaacc tgagctttct ccgcctcttc cgtgctgccc gactcatcaa actcctccgc
     9421 cagggttaca ccatccgcat tctcctctgg actttcgtgc agtctttcaa ggccctacct
     9481 tatgtctgtc tgctgatcgc catgctcttc ttcatctatg ccatcatcgg gatgcaggtg
     9541 tttggcaaca tcggcattga tggggaagat gaggacagcg atgaggatga gttccaaatc
     9601 acgaggcaca ataacttccg gaccttcttc caagctctca tgcttctctt tcggagcgcc
     9661 acaggggcag cgtggcacaa catcatgctg tcctgcctca gcgggaagcc atgcgacaag
     9721 aactccggga tccaaaaacc agagtgtggc aacgagttcg cctattttta ctttgtctcg
     9781 ttcatcttcc tttgctcatt tctgatgctg aatctctttg ttgctgtcat catggacaac
     9841 ttcgagtacc tcacccgaga ttcctccatc ctgggccccc accacctgga tgagtacgtg
     9901 cgtgtctggg cagagtatga ccctgctgcc tgcggccgca ttcactataa ggacatgtac
     9961 agtttattgc gagtaatatc gccccctctc ggcttaggca agaaatgtcc tcatagggtt
    10021 gcttgcaaga ggctcttgcg gatggaccta cccgtagcgg atgacaacac cgttcacttc
    10081 aactccacct tgatggctct gatccgaacc gccctggata tcaaaatcgc aaagggtgga
    10141 gctgacaagc agcaaatgga cgcagagctc cgcaaggaaa tgatggccat ttggcccaac
    10201 ctgtctcaga agaccttgga tctgctggtc acacctcaca agtccacgga cctgacagtg
    10261 ggtaagatct acgcagccat gatgatcatg gagtactacc ggcagagcaa ggccaagaag
    10321 ctgcaggcca tgcgagagga gcagaaccgg acaccactca tgttccagcg catggagcct
    10381 ccatcgccaa cacaggaggg aggacccagc caaaacgccc ttccctccac tcagctggac
    10441 cccggaggag gcctgatggc tcaagaaagc agcatgaagg agagcccgtc ctgggtgacc
    10501 cagcgggcac aggagatgtt ccagaagact ggtacctgga gcccagagcg agggccaccc
    10561 atcgacatgc ctaacagcca gcccaactcc cagtctgtgg agatgagaga aatgggaact
    10621 gatggctact cagacagcga acactacctc cccatggaag gacagaccag ggccgcctcc
    10681 atgccccgcc tcccagcaga gaaccagagg agaaggggcc ggccacgtgg aaataacctc
    10741 agtaccatct ctgataccag ccccatgaag cgctcagcct ccgtgctggg acccaaagcc
    10801 cggcgactgg atgactactc actagagcgg gtaccacctg aggagaacca aaggtaccac
    10861 caacgccgcc gggaccgtgg ccaccgcacc tctgagcgct ctctgggccg atacactgat
    10921 gtggacacag gcctggggac agatctgagc atgaccaccc aatcgggtga cctgccctcc
    10981 aaagatcggg accaggaccg gggccggccc aaggaccgga agcatcggcc acaccaccac
    11041 caccaccatc atcaccatca tcccccggcc ccggaccggg agcgctacgc acaggagcgg
    11101 ccggacaccg gccgggcgcg ggcccgggag cagcgctggt cccgctcgcc cagcgagggt
    11161 cgggagcacg cgacacacag acagtagttc cgtcagtgga agcccagccc cctctacgtc
    11221 cggcaccagc acgccgcggc gcggccgccg ccagctcccc cagaccccct gcaccccgcg
    11281 gccgcttgtg tcctactcgc ccgctccgcg caggcctgcg gcgcgcagga tggcgggtcc
    11341 cccagcgccc cctggcggct cgccgcgggg ttgccgccgt gcgccgcgct ggcctgcgca
    11401 tgcgcctgag ggcccgcggc cgcgcggtgc cgactacacc gagcccgaca gcccgcgcga
    11461 gccgccaggg ggcgcgcatg agcccgcgcc gcggtcgccc aggactccgc gcgccgcggg
    11521 ctgcgcgtca ccgcgccacg gccgcaggct gcccaatggt tactacgcgg ggcacggcgc
    11581 gccacggccg cgcacagccc gcaggggcgc gcacgacgcg tacagcgaga gcgaggacga
    11641 ctggtgctaa gccgccccgc cccccgcagc gcccgtacgg tgcgggcgca ccgcaaatga
    11701 taaacaataa cgtttttaaa aaagaaaacc ggggagaaaa acaaaaatag cttctattga
    11761 tgagttttat catctcgatt gaatctttcg ttttcccggt gaacgaaacc gaggtggctg
    11821 cgagccggag ggacggctgc acgccccaca cacttctcag acccacggga gttcttgcgg
    11881 gccacgccaa acttattact gcctgcagag ttcaactaca agaaaataac actaacagtt
    11941 tttaaaagga ctccgtaaaa agaaatttga aagagaatgt agagaggctg agaaagccca
    12001 ggaattcctg cagcccgggg gatccactag agggccctat tctatagtgt cacctaaatg
    12061 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
    12121 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
    12181 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
    12241 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
    12301 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
    12361 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
    12421 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
    12481 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
    12541 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
    12601 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
    12661 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
    12721 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
    12781 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
    12841 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
    12901 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
    12961 gcccctaact ccgcccatcc cgcccctaac tc
//
