LOCUS       sequence-410291-        6407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Rab11a belongs to the Rab family of small GTPases, Rab11 proteins 
            are particularly involved in trafficking through recycling 
            endosomes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Tran-Van-Minh A, Dolphin AC
  TITLE     The alpha2delta ligand gabapentin inhibits the Rab11-dependent 
            recycling of the calcium channel subunit alpha2delta-2.
  JOURNAL   J Neurosci. 2010 Sep 22;30(38):12856-67. doi: 
            10.1523/JNEUROSCI.2700-10.2010.
  PUBMED    20861389
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2700-10"; journalName: "J Neurosci"; date: 
            "2010-09-22- 22"; volume: "30"; issue: "38"; pages: "12856-67"
COMMENT     Sequence Label: EGFP Rab11a WT pcDNA3.1 Zeo (+)
FEATURES             Location/Qualifiers
     source          1..6407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      149..284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     211..230
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        346..393
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             412..786
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    916..1037
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(953..972)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1007..1026
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1086..1102)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1086..1102)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1099..1121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1110..1126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1134..1140,1141..1158,1159..1164))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1179..1200
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1317..1334)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1488..2076)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1568..1587)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2247..3038,3039..3107))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2870..2889
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3108..3212)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3287..3306)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3478..3857
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3858..4061
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4012..4032
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4106..4125
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4106..4124
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4147..4156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4153..4155,4156..4158,4159..4869)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4198..4219)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4459..4478)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4806..4827
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5658..5675)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5664..5888
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5934..6362
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6021..6040)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6231..6252
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6371..6391)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(6376..6407,1..298)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
       61 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      121 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      181 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc
      241 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
      301 gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca
      361 tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag
      421 ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg
      481 accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg
      541 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg
      601 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc
      661 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg
      721 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag
      781 gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc
      841 ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag
      901 ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc
      961 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     1021 ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa
     1081 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1141 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     1201 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     1261 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     1321 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1381 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1441 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1501 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1561 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1621 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1681 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1741 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1801 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1861 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1921 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1981 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     2041 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     2101 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2161 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2221 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     2281 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     2341 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     2401 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     2461 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     2521 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     2581 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     2641 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     2701 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     2761 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     2821 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     2881 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     2941 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     3001 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     3061 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     3121 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     3181 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     3241 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
     3301 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
     3361 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
     3421 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
     3481 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
     3541 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
     3601 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
     3661 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
     3721 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
     3781 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
     3841 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
     3901 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
     3961 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
     4021 gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta actagagaac
     4081 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     4141 agctaggcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4201 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4261 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4321 ccctggccca ccctcgtgac caccctgagc tacggcgtgc agtgcttcag ccgctacccc
     4381 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4441 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4501 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4561 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4621 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4681 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4741 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4801 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4861 ctgtacaagt ccggactcag atctcggcga gctctcgaga attctcacgc gtctgcagga
     4921 tatcaagctt gcggtaccgc gggcccggga tccaccatgg gcacccgcga cgacgagtac
     4981 gactacctct ttaaagttgt ccttattgga gattctggtg ttggaaagag taatctcctg
     5041 tctcgattta ctcgaaatga gtttaatctg gaaagcaaga gcaccattgg agtagagttt
     5101 gcaacaagaa gcatccaggt tgatggaaaa acaataaagg cacagatatg ggacacagca
     5161 gggcaagagc gatatcgagc tataacatca gcatattatc gtggagctgt aggtgcctta
     5221 ttggtttatg acattgctaa acatctcaca tatgaaaatg tagagcgatg gctgaaagaa
     5281 ctgagagatc atgctgatag taacattgtt atcatgcttg tgggcaataa gagtgatcta
     5341 cgtcatctca gggcagttcc tacagatgaa gcaagagctt ttgcagaaaa gaatggtttg
     5401 tcattcattg aaacttcggc cctagactct acaaatgtag aagctgcttt tcagacaatt
     5461 ttaacagaga tttaccgcat tgtttctcag aagcaaatgt cagacagacg cgaaaatgac
     5521 atgtctccaa gcaacaatgt ggttcctatt catgttccac caaccactga aaacaagcca
     5581 aaggtgcagt gctgtcagaa catctaactc gaggcggccg ctcgagtcta gagggcccgt
     5641 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
     5701 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
     5761 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
     5821 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
     5881 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
     5941 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6001 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6061 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6121 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6181 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6241 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6301 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6361 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccagg
//
