LOCUS       sequence-410293-        6407 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dominant-negative Rab11a mutant - S25N mutation causes inhibition of
            Rab11a-dependent recycling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Tran-Van-Minh A, Dolphin AC
  TITLE     The alpha2delta ligand gabapentin inhibits the Rab11-dependent 
            recycling of the calcium channel subunit alpha2delta-2.
  JOURNAL   J Neurosci. 2010 Sep 22;30(38):12856-67. doi: 
            10.1523/JNEUROSCI.2700-10.2010.
  PUBMED    20861389
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.2700-10"; journalName: "J Neurosci"; date: 
            "2010-09-22- 22"; volume: "30"; issue: "38"; pages: "12856-67"
COMMENT     Sequence Label: EGFP Rab11a S25N pcDNA3.1 Zeo (+)
FEATURES             Location/Qualifiers
     source          1..6407
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    354..475
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(391..410)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     445..464
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(524..540)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(524..540)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(537..559)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    548..564
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(572..578,579..596,597..602))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    617..638
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(755..772)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(926..1514)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1006..1025)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1685..2476,2477..2545))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2308..2327
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2546..2650)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2725..2744)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2916..3295
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3296..3499
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3450..3470
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3544..3563
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3544..3562
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      3585..3594
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3591..3593,3594..3596,3597..4307)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3636..3657)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3897..3916)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4244..4265
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5096..5113)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5102..5326
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5372..5800
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5459..5478)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5669..5690
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5809..5829)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5814..6143
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5994..6129
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6056..6075
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6191..6238
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             join(6257..6407,1..224)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc
       61 tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg gacgcctccg
      121 ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc
      181 cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg ctacgagatt
      241 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
      301 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
      361 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
      421 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
      481 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
      541 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
      601 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
      661 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
      721 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
      781 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
      841 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
      901 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
      961 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1021 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1081 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1141 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1201 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1261 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     1321 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     1381 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     1441 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     1501 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     1561 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     1621 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     1681 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     1741 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     1801 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     1861 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     1921 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     1981 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2041 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2101 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2161 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2221 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     2281 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     2341 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     2401 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     2461 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     2521 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     2581 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     2641 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     2701 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     2761 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     2821 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     2881 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     2941 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     3001 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     3061 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     3121 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     3181 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     3241 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     3301 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     3361 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     3421 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     3481 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     3541 aattaatacg actcactata gggagaccca agctggctag ctaggccacc atggtgagca
     3601 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3661 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3721 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3781 ccctgagcta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3841 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3901 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3961 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     4021 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     4081 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4141 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4201 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4261 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtcc ggactcagat
     4321 ctcggcgagc tctcgagaat tctcacgcgt ctgcaggata tcaagcttgc ggtaccgcgg
     4381 gcccgggatc caccatgggc acccgcgacg acgagtacga ctacctcttt aaagttgtcc
     4441 ttattggaga ttctggtgtt ggaaagaata atctcctgtc tcgatttact cgaaatgagt
     4501 ttaatctgga aagcaagagc accattggag tagagtttgc aacaagaagc atccaggttg
     4561 atggaaaaac aataaaggca cagatatggg acacagcagg gcaagagcga tatcgagcta
     4621 taacatcagc atattatcgt ggagctgtag gtgccttatt ggtttatgac attgctaaac
     4681 atctcacata tgaaaatgta gagcgatggc tgaaagaact gagagatcat gctgatagta
     4741 acattgttat catgcttgtg ggcaataaga gtgatctacg tcatctcagg gcagttccta
     4801 cagatgaagc aagagctttt gcagaaaaga atggtttgtc attcattgaa acttcggccc
     4861 tagactctac aaatgtagaa gctgcttttc agacaatttt aacagagatt taccgcattg
     4921 tttctcagaa gcaaatgtca gacagacgcg aaaatgacat gtctccaagc aacaatgtgg
     4981 ttcctattca tgttccacca accactgaaa acaagccaaa ggtgcagtgc tgtcagaaca
     5041 tctaactcga ggcggccgct cgagtctaga gggcccgttt aaacccgctg atcagcctcg
     5101 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     5161 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     5221 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     5281 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     5341 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     5401 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5461 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5521 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5581 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5641 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5701 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5761 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5821 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     5881 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     5941 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     6001 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     6061 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     6121 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     6181 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     6241 ggtgaggaac taaaccatgg ccaagttgac cagtgccgtt ccggtgctca ccgcgcgcga
     6301 cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg acttcgtgga
     6361 ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatca
//
