LOCUS       sequence-410294-       12992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 calcium channel with a E1686R mutation to 
            enhance expression and a mutation in the P loop of Domain I to 
            abolish divalent cation binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.1 HA EIA (E320A) pcDNA3
FEATURES             Location/Qualifiers
     source          1..12992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             106..900
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(160..179)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     770..789
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1074..1195
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1111..1130)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1165..1184
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1244..1260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1244..1260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1257..1279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1268..1284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1292..1298,1299..1316,1317..1322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1337..1358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1475..1492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1646..2234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1726..1745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2405..3196,3197..3265))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3028..3047
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3266..3370)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3445..3464)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3636..4015
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4016..4219
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4170..4190
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4264..4283
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4264..4282
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6104..6130
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6134..6160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10937..10966
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(11946..11964)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11947..11964)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11984..12001)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11990..12214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12260..12688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12347..12366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12557..12578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12697..12717)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12702..12992,1..39)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12882..12992,1..25)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12944..12963
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
       61 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
      121 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
      181 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
      241 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
      301 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
      361 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
      421 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
      481 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
      541 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
      601 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
      661 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
      721 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
      781 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
      841 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
      901 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
      961 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     1021 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     1081 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     1141 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     1201 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     1261 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     1321 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     1381 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     1441 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1501 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1561 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1621 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1681 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1741 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1801 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     1861 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1921 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     1981 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2041 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     2101 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2161 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2221 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2281 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2341 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2401 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2461 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2521 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2581 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2641 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2701 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2761 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2821 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2881 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     2941 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3001 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     3061 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3121 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3181 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3241 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3301 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3361 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     3421 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     3481 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     3541 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     3601 gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
     3661 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     3721 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     3781 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     3841 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     3901 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     3961 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     4021 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     4081 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
     4141 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     4201 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
     4261 aattaatacg actcactata gggagaccca agcttggtac cgagctcgga tccactagta
     4321 acggccgcca gtgtgctgga attctgcaga tatccatcac actggcggcc gctcgaggtc
     4381 ttgcagaatg gcccgctttg gagacgagat gccgggccgc tacggcgcag gcggaggagg
     4441 ctcagggccg gccgccgggg tggtcgtggg cgccgcgggc ggccgaggag ccgggggcag
     4501 ccggcagggc gggcagcccg gagcgcagag gatgtacaag cagtcgatgg cgcagagagc
     4561 gcggaccatg gccctctaca accccatccc tgtccgccag aactgcctca cggtcaaccg
     4621 ctccctgttc ctcttcagtg aagacaacgt ggtgagaaaa tacgccaaaa agatcacgga
     4681 atggcctccc ttcgagtaca tgatcctggc caccatcatt gctaactgca tcgtcctggc
     4741 cctggagcag cacctccctg atgatgacaa gacacccatg tccgagcggc tggatgacac
     4801 agaaccctat ttcattggca tcttctgttt tgaggctgga attaagatcg tggctcttgg
     4861 ctttgccttc cacaaaggct cctacctgag gaatggctgg aacgtcatgg actttgtcgt
     4921 ggtgctaaca ggcatcttgg ccactgtcgg gacggagttt gatctacgga cactgagggc
     4981 ggttcgtgtg ctgcggccac tcaagctggt gtctggaatc ccaagtttac aagtcgtcct
     5041 gaagtcaatc atgaaggcga tgatccctct gctgcagatc ggcctcctcc tgttttttgc
     5101 aatccttatt tttgcaatca tagggttaga attttatatg ggaaaatttc ataccacctg
     5161 ctttgaagag gggacagacg acatccaggg tgagtcgcca gctccgtgtg ggacagagga
     5221 gcctgcccgc acctgcccca acgggaccaa atgtcagccg tactgggaag ggcccaacaa
     5281 cggcatcact cagttcgaca acatcctgtt tgctgtgctc actgttttcc agtgcatcac
     5341 catggcaggc tggactgatc tcctctacaa tagcaacgat gcctcaggga acacttggaa
     5401 ctggttgtac ttcatccccc tcatcatcat cggctccttt tttatgctga accttgtgct
     5461 gggtgtgctg tctggggagt ttgccaaaga aagggaacgt gtagagaacc gaagggcttt
     5521 tctgaagctc agaagacaac agcagattga acgtgagctc aatggataca tggagtggat
     5581 ctcgaaagca gaagaggtga tcctcgcgga ggacgaaaca gacgtggagc agaggcaccc
     5641 ttttgatgga gctcttcgga gagctactct gaagaaaagc aagacggacc tgctcaaccc
     5701 tgaggaggcg gaggaccagc ttgctgacat cgcgtctgtg gggtctccct tcgccagagc
     5761 cagcatcaaa agtgccaagc tggagaattc gacttttttc cacaaaaagg agagaagaat
     5821 gcgtttctac atccgccgca tggtcaaaac tcaggccttc tactggaccg tgctcagtct
     5881 ggtagccctc aacacgctgt gcgtcgccat tgtccactac aaccagcccg agtggctctc
     5941 cgacttcctc tactatgcag aattcatttt cttaggactc tttatgtccg aaatgtttat
     6001 aaaaatgtat gggctcggga cacggcctta cttccactct tccttcaact gctttgactg
     6061 tggggtcatc atcgggagca tctttgaagt catctgggcc gtctacccct acgacgtccc
     6121 cgactacgcc ggctacccgt acgacgtccc ggactacgcc ggcgtcgaca tcaaaccggg
     6181 tacatccttt ggaatcagcg tgttacgagc tctcaggtta ctgcgtattt tcaaagtcac
     6241 aaagtactgg gcatctctca gaaacctggt tgtctccctc ctcaactcca tgaaatccat
     6301 cataagtctg ctgttcctcc tcttcctctt cattgtcgtc tttgccctct tggggatgca
     6361 gctgtttggt ggccagttta attttgacga ggggactcct cccaccaact tcgacacttt
     6421 tccagcagca ataatgactg tgtttcagat cctgactggc gaggattgga atgaggtcat
     6481 gtatgatggg atcaagtctc aggggggcgt gcagggcggc atggtgttct ccatctactt
     6541 catcgtcctc accctcttcg ggaactacac cctgctgaac gtgttcttgg ctatcgcggt
     6601 ggacaacctg gccaacgccc aggaactcac caaggatgaa caagaagagg aagaggcagc
     6661 caatcagaaa ctggctctac agaaagccaa ggaggtggca gaagtgagtc ccctgtctgc
     6721 agccaacatg tccatagctg tgaaggaaca gcagaagaac cagaagcctg ccaagtcggt
     6781 gtgggagcag cgcaccagcg agatgcgcaa gcagaacctg ctggctagcc gcgaggcgct
     6841 gtacggggac gcggctgagc gctggcccac cacttacgcg cgcccgctgc ggccggacgt
     6901 gaagacgcac ttggaccggc cgctcgtggt ggacccgcag gagaaccgta acaacaacac
     6961 caacaagagc cgtgcgccag aagcgctgcg ccaaaccgcg cggccccgcg agagcgcgcg
     7021 cgaccccgac gcgcggcgcg cctggcccag cagccctgag cgcgcccctg gacgagaggg
     7081 cccgtatggc cgcgagagcg agccgcaaca gcgcgagcac gcgccacccc gcgagcacgt
     7141 accctgggac gcggatcctg agcgcgccaa ggccggggac gcgccccgcc gccacacgca
     7201 ccggcctgtg gccgagggcg agcctcgtcg ccaccgcgcg cgccgccggc ccggggacga
     7261 accggacgac agaccggagc gcaggccgcg tccccgcgac gccactaggc cggcccgcgc
     7321 tgcagacggc gaaggcgatg atggggagcg caagcggcga caccgacacg ggccgccggc
     7381 ccacgatgac agggagcgca gacaccggcg cagaaaagag agccagggct ctggggtccc
     7441 catgtctggt cccaacctgt ccaccaccag gccaatccag caggatctgg gccgccagga
     7501 cctgccactg gctgaggacc tggacaacat gaagaacaac aagttggcca ccggggagcc
     7561 tgccagtccc cacgacagcc tgggccacag tggccttccc cctagccctg ccaagatcgg
     7621 gaacagcacc aaccctggtc ccgccttggc caccaatccc cagaatgctg ccagccgcag
     7681 gacgcccaac aacccgggca acccgtccaa ccccggcccc cccaagactc ccgagaacag
     7741 ccttatcgtc accaacccca gcagcaccca gcccaactca gcaaagactg ccaggaaacc
     7801 cgagcacatg gcggtggaga tccccccggc ctgcccgccc ctcaaccaca ctgtggtcca
     7861 agtaaacaaa aacgccaacc cagacccact gccaaagaaa gaggaagaga agaaggagga
     7921 agaggaggca gaccccgggg aggatggccc aaagcccatg ccgccctaca gctccatgtt
     7981 catcctctcc accaccaacc cccttcgccg gctgtgccat tacatcctga acctgcgcta
     8041 cttcgagatg tgcatcctca tggtcattgc catgagtagc atcgcgctgg ccgccgagga
     8101 cccggtgcag cccaacgcac cccgcaacaa cgtgctgcga tattttgact atgttttcac
     8161 aggagtgttt acctttgaga tggtgatcaa gatgatcgac ctgggcctcg tcctgcatca
     8221 gggggcctat ttccgtgacc tgtggaacat tctggacttc atagtggtca gtggggccct
     8281 ggtggccttt gccttcactg gcaatagcaa aggaaaggac atcaacacca tcaagtccct
     8341 ccgagtcctc cgggtgctac gacctctaaa gaccatcaag cggctgccta agttgaaggc
     8401 cgtatttgac tgcgtggtga actcgctcaa gaacgtcttc aacatcctca ttgtctacat
     8461 gctcttcatg ttcatcttcg ccgtggtggc cgtgcagctc ttcaagggca aattcttcca
     8521 ctgcacggac gagtccaagg agtttgagag agactgtcga ggcaaatacc tcctttacga
     8581 gaagaacgag gtaaaggcgc gggaccgcga gtggaagaaa tacgacttcc actacgacaa
     8641 cgtgctctgg gccctgctca cgctctttac ggtgtccacg ggagagggct ggccacaggt
     8701 cctcaagcac tcagtggatg ccacttttga gaaccagggc cccagccccg ggtaccgcat
     8761 ggaaatgtcc atcttctacg tggtctactt tgtggtgttt cccttcttct ttgtcaatat
     8821 ctttgtggcc ttgatcatca tcaccttcca ggagcaggga gacaagatga tggaagaata
     8881 cagcctagag aaaaatgaga gggcctgcat cgactttgcc atcagtgcca agccgctgac
     8941 caggcacatg ccccagaaca agcagagctt ccagtatcga atgtggcagt tcgtggtgtc
     9001 cccacccttt gagtacacca tcatggccat gatcgctctc aacaccatcg tgctaatgat
     9061 gaagttctat ggagcctctg tggcctatga aaacgccctt cgagtgttca acattgtctt
     9121 cacctccctc ttctctctcg aatgtgtgct caaagtcatg gcttttggga ttctgaatta
     9181 tttccgcgat gcctggaaca tcttcgactt tgtgactgtt ctgggcagca tcacagacat
     9241 cctcgtcacc gagtttggga ataacttcat caacctgagc tttctccgcc tcttccgtgc
     9301 tgcccgactc atcaaactcc tccgccaggg ttacaccatc cgcattctcc tctggacttt
     9361 cgtgcagtct ttcaaggccc taccttatgt ctgtctgctg atcgccatgc tcttcttcat
     9421 ctatgccatc atcgggatgc aggtgtttgg caacatcggc attgatgggg aagatgagga
     9481 cagcgatgag gatgagttcc aaatcacgag gcacaataac ttccggacct tcttccaagc
     9541 tctcatgctt ctcttccgga gcgccacagg ggaagcgtgg cacaacatca tgctgtcctg
     9601 cctcagcggg aagccatgcg acaagaactc cgggatccaa aaaccagagt gtggcaacga
     9661 gttcgcctat ttttactttg tctcgttcat cttcctttgc tcatttctga tgctgaatct
     9721 ctttgttgct gtcatcatgg acaacttcga gtacctcacc cgagattcct ccatcctggg
     9781 cccccaccac ctggatgagt acgtgcgtgt ctgggcagag tatgaccctg ctgcctgcgg
     9841 ccgcattcac tataaggaca tgtacagttt attgcgagta atatcgcccc ctctcggctt
     9901 aggcaagaaa tgtcctcata gggttgcttg caagaggctc ttgcggatgg acctacccgt
     9961 agcggatgac aacaccgttc acttcaactc caccttgatg gctctgatcc gaaccgccct
    10021 ggatatcaaa atcgcaaagg gtggagctga caagcagcaa atggacgcag agctccgcaa
    10081 ggaaatgatg gccatttggc ccaacctgtc tcagaagacc ttggatctgc tggtcacacc
    10141 tcacaagtcc acggacctga cagtgggtaa gatctacgca gccatgatga tcatggagta
    10201 ctaccggcag agcaaggcca agaagctgca ggccatgcga gaggagcaga accggacacc
    10261 actcatgttc cagcgcatgg agcctccatc gccaacacag gagggaggac ccagccaaaa
    10321 cgcccttccc tccactcagc tggaccccgg aggaggcctg atggctcaag aaagcagcat
    10381 gaaggagagc ccgtcctggg tgacccagcg ggcacaggag atgttccaga agactggtac
    10441 ctggagccca gagcgagggc cacccatcga catgcctaac agccagccca actcccagtc
    10501 tgtggagatg agagaaatgg gaactgatgg ctactcagac agcgaacact acctccccat
    10561 ggaaggacag accagggccg cctccatgcc ccgcctccca gcagagaacc agaggagaag
    10621 gggccggcca cgtggaaata acctcagtac catctctgat accagcccca tgaagcgctc
    10681 agcctccgtg ctgggaccca aagcccggcg actggatgac tactcactag agcgggtacc
    10741 acctgaggag aaccaaaggt accaccaacg ccgccgggac cgtggccacc gcacctctga
    10801 gcgctctctg ggccgataca ctgatgtgga cacaggcctg gggacagatc tgagcatgac
    10861 cacccaatcg ggtgacctgc cctccaaaga tcgggaccag gaccggggcc ggcccaagga
    10921 ccggaagcat cggccacacc accaccacca ccatcatcac catcatcccc cggccccgga
    10981 ccgggagcgc tacgcacagg agcggccgga caccggccgg gcgcgggccc gggagcagcg
    11041 ctggtcccgc tcgcccagcg agggtcggga gcacgcgaca cacagacagt agttccgtca
    11101 gtggaagccc agccccctct acgtccggca ccagcacgcc gcggcgcggc cgccgccagc
    11161 tcccccagac cccctgcacc ccgcggccgc ttgtgtccta ctcgcccgct ccgcgcaggc
    11221 ctgcggcgcg caggatggcg ggtcccccag cgccccctgg cggctcgccg cggggttgcc
    11281 gccgtgcgcc gcgctggcct gcgcatgcgc ctgagggccc gcggccgcgc ggtgccgact
    11341 acaccgagcc cgacagcccg cgcgagccgc cagggggcgc gcatgagccc gcgccgcggt
    11401 cgcccaggac tccgcgcgcc gcgggctgcg cgtcaccgcg ccacggccgc aggctgccca
    11461 atggttacta cgcggggcac ggcgcgccac ggccgcgcac agcccgcagg ggcgcgcacg
    11521 acgcgtacag cgagagcgag gacgactggt gctaagccgc cccgcccccc gcagcgcccg
    11581 tacggtgcgg gcgcaccgca aatgataaac aataacgttt ttaaaaaaga aaaccgggga
    11641 gaaaaacaaa aatagcttct attgatgagt tttatcatct cgattgaatc tttcgttttc
    11701 ccggtgaacg aaaccgaggt ggctgcgagc cggagggacg gctgcacgcc ccacacactt
    11761 ctcagaccca cgggagttct tgcgggccac gccaaactta ttactgcctg cagagttcaa
    11821 ctacaagaaa ataacactaa cagtttttaa aaggactccg taaaaagaaa tttgaaagag
    11881 aatgtagaga ggctgagaaa gcccaggaat tcctgcagcc cgggggatcc actagagggc
    11941 cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac tgtgccttct
    12001 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
    12061 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
    12121 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
    12181 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
    12241 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    12301 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    12361 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    12421 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    12481 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    12541 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    12601 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    12661 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
    12721 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
    12781 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
    12841 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
    12901 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    12961 aggccgcctc tgcctctgag ctattccaga ag
//
