LOCUS       sequence-410308-       12992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of rat Cav2.1 calcium channel with a E1686R mutation to 
            enhance expression and a mutation in the P loop of Domain IV to to 
            abolish divalent cation binding.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Meyer JO, Dahimene S, Page KM, Ferron L, Kadurin I, Ellaway JIJ, 
            Zhao P, Patel T, Rothwell SW, Lin P, Pratt WS, Dolphin AC
  TITLE     Disruption of the Key Ca(2+) Binding Site in the Selectivity Filter 
            of Neuronal Voltage-Gated Calcium Channels Inhibits Channel 
            Trafficking.
  JOURNAL   Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi: 
            10.1016/j.celrep.2019.08.079.
  PUBMED    31577951
REFERENCE   2  (bases 1 to 12992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.08"; journalName: "Cell Rep"; date: 
            "2019-10-1- 1"; volume: "29"; issue: "1"; pages: "22-33.e5"
COMMENT     Sequence Label: Cav2.1 EIVA (E1707A) pcDNA3
FEATURES             Location/Qualifiers
     source          1..12992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             116..910
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(170..189)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     780..799
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1084..1205
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1121..1140)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1175..1194
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1254..1270)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1254..1270)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1267..1289)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1278..1294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1302..1308,1309..1326,1327..1332))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1347..1368
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1485..1502)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1656..2244)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1736..1755)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2415..3206,3207..3275))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3038..3057
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3276..3380)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3455..3474)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3646..4025
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4026..4229
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4180..4200
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4274..4293
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4274..4292
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6114..6140
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6144..6170
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10947..10976
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     promoter        complement(11956..11974)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11957..11974)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11994..12011)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12000..12224
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12270..12698
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12357..12376)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12567..12588
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12707..12727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(12712..12992,1..49)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(12892..12992,1..35)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12954..12973
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
       61 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
      121 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
      181 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
      241 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
      301 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
      361 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
      421 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
      481 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
      541 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
      601 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
      661 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
      721 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
      781 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
      841 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
      901 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
      961 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     1021 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     1081 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1141 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1201 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     1261 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     1321 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     1381 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     1441 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     1501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     2101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2701 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     2761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3001 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     3061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     3421 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3481 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     3541 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
     3601 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
     3661 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     3721 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     3781 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3841 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3901 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3961 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     4021 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     4081 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     4141 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     4201 tacggtggga ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact
     4261 ggcttatcga aattaatacg actcactata gggagaccca agcttggtac cgagctcgga
     4321 tccactagta acggccgcca gtgtgctgga attctgcaga tatccatcac actggcggcc
     4381 gctcgaggtc ttgcagaatg gcccgctttg gagacgagat gccgggccgc tacggcgcag
     4441 gcggaggagg ctcagggccg gccgccgggg tggtcgtggg cgccgcgggc ggccgaggag
     4501 ccgggggcag ccggcagggc gggcagcccg gagcgcagag gatgtacaag cagtcgatgg
     4561 cgcagagagc gcggaccatg gccctctaca accccatccc tgtccgccag aactgcctca
     4621 cggtcaaccg ctccctgttc ctcttcagtg aagacaacgt ggtgagaaaa tacgccaaaa
     4681 agatcacgga atggcctccc ttcgagtaca tgatcctggc caccatcatt gctaactgca
     4741 tcgtcctggc cctggagcag cacctccctg atgatgacaa gacacccatg tccgagcggc
     4801 tggatgacac agaaccctat ttcattggca tcttctgttt tgaggctgga attaagatcg
     4861 tggctcttgg ctttgccttc cacaaaggct cctacctgag gaatggctgg aacgtcatgg
     4921 actttgtcgt ggtgctaaca ggcatcttgg ccactgtcgg gacggagttt gatctacgga
     4981 cactgagggc ggttcgtgtg ctgcggccac tcaagctggt gtctggaatc ccaagtttac
     5041 aagtcgtcct gaagtcaatc atgaaggcga tgatccctct gctgcagatc ggcctcctcc
     5101 tgttttttgc aatccttatt tttgcaatca tagggttaga attttatatg ggaaaatttc
     5161 ataccacctg ctttgaagag gggacagacg acatccaggg tgagtcgcca gctccgtgtg
     5221 ggacagagga gcctgcccgc acctgcccca acgggaccaa atgtcagccg tactgggaag
     5281 ggcccaacaa cggcatcact cagttcgaca acatcctgtt tgctgtgctc actgttttcc
     5341 agtgcatcac catggaaggc tggactgatc tcctctacaa tagcaacgat gcctcaggga
     5401 acacttggaa ctggttgtac ttcatccccc tcatcatcat cggctccttt tttatgctga
     5461 accttgtgct gggtgtgctg tctggggagt ttgccaaaga aagggaacgt gtagagaacc
     5521 gaagggcttt tctgaagctc agaagacaac agcagattga acgtgagctc aatggataca
     5581 tggagtggat ctcgaaagca gaagaggtga tcctcgcgga ggacgaaaca gacgtggagc
     5641 agaggcaccc ttttgatgga gctcttcgga gagctactct gaagaaaagc aagacggacc
     5701 tgctcaaccc tgaggaggcg gaggaccagc ttgctgacat cgcgtctgtg gggtctccct
     5761 tcgccagagc cagcatcaaa agtgccaagc tggagaattc gacttttttc cacaaaaagg
     5821 agagaagaat gcgtttctac atccgccgca tggtcaaaac tcaggccttc tactggaccg
     5881 tgctcagtct ggtagccctc aacacgctgt gcgtcgccat tgtccactac aaccagcccg
     5941 agtggctctc cgacttcctc tactatgcag aattcatttt cttaggactc tttatgtccg
     6001 aaatgtttat aaaaatgtat gggctcggga cacggcctta cttccactct tccttcaact
     6061 gctttgactg tggggtcatc atcgggagca tctttgaagt catctgggcc gtctacccct
     6121 acgacgtccc cgactacgcc ggctacccgt acgacgtccc ggactacgcc ggcgtcgaca
     6181 tcaaaccggg tacatccttt ggaatcagcg tgttacgagc tctcaggtta ctgcgtattt
     6241 tcaaagtcac aaagtactgg gcatctctca gaaacctggt tgtctccctc ctcaactcca
     6301 tgaaatccat cataagtctg ctgttcctcc tcttcctctt cattgtcgtc tttgccctct
     6361 tggggatgca gctgtttggt ggccagttta attttgacga ggggactcct cccaccaact
     6421 tcgacacttt tccagcagca ataatgactg tgtttcagat cctgactggc gcggattgga
     6481 atgaggtcat gtatgatggg atcaagtctc aggggggcgt gcagggcggc atggtgttct
     6541 ccatctactt catcgtcctc accctcttcg ggaactacac cctgctgaac gtgttcttgg
     6601 ctatcgcggt ggacaacctg gccaacgccc aggaactcac caaggatgaa caagaagagg
     6661 aagaggcagc caatcagaaa ctggctctac agaaagccaa ggaggtggca gaagtgagtc
     6721 ccctgtctgc agccaacatg tccatagctg tgaaggaaca gcagaagaac cagaagcctg
     6781 ccaagtcggt gtgggagcag cgcaccagcg agatgcgcaa gcagaacctg ctggctagcc
     6841 gcgaggcgct gtacggggac gcggctgagc gctggcccac cacttacgcg cgcccgctgc
     6901 ggccggacgt gaagacgcac ttggaccggc cgctcgtggt ggacccgcag gagaaccgta
     6961 acaacaacac caacaagagc cgtgcgccag aagcgctgcg ccaaaccgcg cggccccgcg
     7021 agagcgcgcg cgaccccgac gcgcggcgcg cctggcccag cagccctgag cgcgcccctg
     7081 gacgagaggg cccgtatggc cgcgagagcg agccgcaaca gcgcgagcac gcgccacccc
     7141 gcgagcacgt accctgggac gcggatcctg agcgcgccaa ggccggggac gcgccccgcc
     7201 gccacacgca ccggcctgtg gccgagggcg agcctcgtcg ccaccgcgcg cgccgccggc
     7261 ccggggacga accggacgac agaccggagc gcaggccgcg tccccgcgac gccactaggc
     7321 cggcccgcgc tgcagacggc gaaggcgatg atggggagcg caagcggcga caccgacacg
     7381 ggccgccggc ccacgatgac agggagcgca gacaccggcg cagaaaagag agccagggct
     7441 ctggggtccc catgtctggt cccaacctgt ccaccaccag gccaatccag caggatctgg
     7501 gccgccagga cctgccactg gctgaggacc tggacaacat gaagaacaac aagttggcca
     7561 ccggggagcc tgccagtccc cacgacagcc tgggccacag tggccttccc cctagccctg
     7621 ccaagatcgg gaacagcacc aaccctggtc ccgccttggc caccaatccc cagaatgctg
     7681 ccagccgcag gacgcccaac aacccgggca acccgtccaa ccccggcccc cccaagactc
     7741 ccgagaacag ccttatcgtc accaacccca gcagcaccca gcccaactca gcaaagactg
     7801 ccaggaaacc cgagcacatg gcggtggaga tccccccggc ctgcccgccc ctcaaccaca
     7861 ctgtggtcca agtaaacaaa aacgccaacc cagacccact gccaaagaaa gaggaagaga
     7921 agaaggagga agaggaggca gaccccgggg aggatggccc aaagcccatg ccgccctaca
     7981 gctccatgtt catcctctcc accaccaacc cccttcgccg gctgtgccat tacatcctga
     8041 acctgcgcta cttcgagatg tgcatcctca tggtcattgc catgagtagc atcgcgctgg
     8101 ccgccgagga cccggtgcag cccaacgcac cccgcaacaa cgtgctgcga tattttgact
     8161 atgttttcac aggagtgttt acctttgaga tggtgatcaa gatgatcgac ctgggcctcg
     8221 tcctgcatca gggggcctat ttccgtgacc tgtggaacat tctggacttc atagtggtca
     8281 gtggggccct ggtggccttt gccttcactg gcaatagcaa aggaaaggac atcaacacca
     8341 tcaagtccct ccgagtcctc cgggtgctac gacctctaaa gaccatcaag cggctgccta
     8401 agttgaaggc cgtatttgac tgcgtggtga actcgctcaa gaacgtcttc aacatcctca
     8461 ttgtctacat gctcttcatg ttcatcttcg ccgtggtggc cgtgcagctc ttcaagggca
     8521 aattcttcca ctgcacggac gagtccaagg agtttgagag agactgtcga ggcaaatacc
     8581 tcctttacga gaagaacgag gtaaaggcgc gggaccgcga gtggaagaaa tacgacttcc
     8641 actacgacaa cgtgctctgg gccctgctca cgctctttac ggtgtccacg ggagagggct
     8701 ggccacaggt cctcaagcac tcagtggatg ccacttttga gaaccagggc cccagccccg
     8761 ggtaccgcat ggaaatgtcc atcttctacg tggtctactt tgtggtgttt cccttcttct
     8821 ttgtcaatat ctttgtggcc ttgatcatca tcaccttcca ggagcaggga gacaagatga
     8881 tggaagaata cagcctagag aaaaatgaga gggcctgcat cgactttgcc atcagtgcca
     8941 agccgctgac caggcacatg ccccagaaca agcagagctt ccagtatcga atgtggcagt
     9001 tcgtggtgtc cccacccttt gagtacacca tcatggccat gatcgctctc aacaccatcg
     9061 tgctaatgat gaagttctat ggagcctctg tggcctatga aaacgccctt cgagtgttca
     9121 acattgtctt cacctccctc ttctctctcg aatgtgtgct caaagtcatg gcttttggga
     9181 ttctgaatta tttccgcgat gcctggaaca tcttcgactt tgtgactgtt ctgggcagca
     9241 tcacagacat cctcgtcacc gagtttggga ataacttcat caacctgagc tttctccgcc
     9301 tcttccgtgc tgcccgactc atcaaactcc tccgccaggg ttacaccatc cgcattctcc
     9361 tctggacttt cgtgcagtct ttcaaggccc taccttatgt ctgtctgctg atcgccatgc
     9421 tcttcttcat ctatgccatc atcgggatgc aggtgtttgg caacatcggc attgatgggg
     9481 aagatgagga cagcgatgag gatgagttcc aaatcacgag gcacaataac ttccggacct
     9541 tcttccaagc tctcatgctt ctcttccgga gcgccacagg ggaagcgtgg cacaacatca
     9601 tgctgtcctg cctcagcggg aagccatgcg acaagaactc cgggatccaa aaaccagagt
     9661 gtggcaacga gttcgcctat ttttactttg tctcgttcat cttcctttgc tcatttctga
     9721 tgctgaatct ctttgttgct gtcatcatgg acaacttcga gtacctcacc cgagattcct
     9781 ccatcctggg cccccaccac ctggatgagt acgtgcgtgt ctgggcagag tatgaccctg
     9841 ctgcctgcgg ccgcattcac tataaggaca tgtacagttt attgcgagta atatcgcccc
     9901 ctctcggctt aggcaagaaa tgtcctcata gggttgcttg caagaggctc ttgcggatgg
     9961 acctacccgt agcggatgac aacaccgttc acttcaactc caccttgatg gctctgatcc
    10021 gaaccgccct ggatatcaaa atcgcaaagg gtggagctga caagcagcaa atggacgcag
    10081 agctccgcaa ggaaatgatg gccatttggc ccaacctgtc tcagaagacc ttggatctgc
    10141 tggtcacacc tcacaagtcc acggacctga cagtgggtaa gatctacgca gccatgatga
    10201 tcatggagta ctaccggcag agcaaggcca agaagctgca ggccatgcga gaggagcaga
    10261 accggacacc actcatgttc cagcgcatgg agcctccatc gccaacacag gagggaggac
    10321 ccagccaaaa cgcccttccc tccactcagc tggaccccgg aggaggcctg atggctcaag
    10381 aaagcagcat gaaggagagc ccgtcctggg tgacccagcg ggcacaggag atgttccaga
    10441 agactggtac ctggagccca gagcgagggc cacccatcga catgcctaac agccagccca
    10501 actcccagtc tgtggagatg agagaaatgg gaactgatgg ctactcagac agcgaacact
    10561 acctccccat ggaaggacag accagggccg cctccatgcc ccgcctccca gcagagaacc
    10621 agaggagaag gggccggcca cgtggaaata acctcagtac catctctgat accagcccca
    10681 tgaagcgctc agcctccgtg ctgggaccca aagcccggcg actggatgac tactcactag
    10741 agcgggtacc acctgaggag aaccaaaggt accaccaacg ccgccgggac cgtggccacc
    10801 gcacctctga gcgctctctg ggccgataca ctgatgtgga cacaggcctg gggacagatc
    10861 tgagcatgac cacccaatcg ggtgacctgc cctccaaaga tcgggaccag gaccggggcc
    10921 ggcccaagga ccggaagcat cggccacacc accaccacca ccatcatcac catcatcccc
    10981 cggccccgga ccgggagcgc tacgcacagg agcggccgga caccggccgg gcgcgggccc
    11041 gggagcagcg ctggtcccgc tcgcccagcg agggtcggga gcacgcgaca cacagacagt
    11101 agttccgtca gtggaagccc agccccctct acgtccggca ccagcacgcc gcggcgcggc
    11161 cgccgccagc tcccccagac cccctgcacc ccgcggccgc ttgtgtccta ctcgcccgct
    11221 ccgcgcaggc ctgcggcgcg caggatggcg ggtcccccag cgccccctgg cggctcgccg
    11281 cggggttgcc gccgtgcgcc gcgctggcct gcgcatgcgc ctgagggccc gcggccgcgc
    11341 ggtgccgact acaccgagcc cgacagcccg cgcgagccgc cagggggcgc gcatgagccc
    11401 gcgccgcggt cgcccaggac tccgcgcgcc gcgggctgcg cgtcaccgcg ccacggccgc
    11461 aggctgccca atggttacta cgcggggcac ggcgcgccac ggccgcgcac agcccgcagg
    11521 ggcgcgcacg acgcgtacag cgagagcgag gacgactggt gctaagccgc cccgcccccc
    11581 gcagcgcccg tacggtgcgg gcgcaccgca aatgataaac aataacgttt ttaaaaaaga
    11641 aaaccgggga gaaaaacaaa aatagcttct attgatgagt tttatcatct cgattgaatc
    11701 tttcgttttc ccggtgaacg aaaccgaggt ggctgcgagc cggagggacg gctgcacgcc
    11761 ccacacactt ctcagaccca cgggagttct tgcgggccac gccaaactta ttactgcctg
    11821 cagagttcaa ctacaagaaa ataacactaa cagtttttaa aaggactccg taaaaagaaa
    11881 tttgaaagag aatgtagaga ggctgagaaa gcccaggaat tcctgcagcc cgggggatcc
    11941 actagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat cagcctcgac
    12001 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
    12061 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
    12121 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
    12181 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
    12241 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
    12301 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    12361 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    12421 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    12481 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    12541 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    12601 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    12661 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
    12721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    12781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    12841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    12901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    12961 gcagaggccg aggccgcctc tgcctctgag ct
//
