LOCUS       sequence-410319-        9748 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral construct for the expression of a FRET-based reporter for
            tau seeding in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   Lathuiliere A, Jo Y, Perbet R, Donahue C, Commins C, Quittot N, Fan 
            Z, Bennett RE, Hyman BT
  TITLE     Specific detection of tau seeding activity in Alzheimer's disease 
            using rationally designed biosensor cells.
  JOURNAL   Mol Neurodegener. 2023 Aug 8;18(1):53. doi: 
            10.1186/s13024-023-00643-2.
  PUBMED    37553663
REFERENCE   2  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Neurodegener."; date: "2023-08-8"; volume: "18(1):53. doi"; pages: "
            10.1186/s13024-023-00643-2"
COMMENT     Sequence Label: pLenti_EF1a_TauFRET_3xKQ_wpre
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    607..628
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(643..648,649..666,667..673)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    681..697
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     686..708
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     705..721
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     705..721
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     740..760
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        742..760
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        788..1014
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1015..1195
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1242..1367
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1860..2093
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2278..2322
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2588..2705
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2723..2743
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2744..3922
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2975..3913
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3870..3890
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3922..3946
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4448..4469)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4709..4728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5056..5077
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5120..5173
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6336..6360)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6378..6966
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6431..6451)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6849..6860)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7054..7287
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7359..7480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7396..7415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7450..7469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7520..7655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7675..7694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7676..7694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7704..7721)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7704..7720)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7713..7735)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7862..8317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7949..7968)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8159..8180
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8343..8447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8448..8516,8517..9308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8666..8685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9479..9748,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaaga gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
      601 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
      661 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
      721 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
      781 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
      841 caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg
      901 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
      961 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     1021 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     1081 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     1141 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1201 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1261 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1321 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1381 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1441 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1501 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1561 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1621 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1681 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1741 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1801 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1861 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1921 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1981 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     2041 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     2101 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     2161 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2221 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2281 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2341 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2401 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2461 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2521 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     2581 cgctagcttt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2641 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2701 attttactag tgattatcgg atcaactttg tatagaaaag ttgggctccg gtgcccgtca
     2761 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg
     2821 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct
     2881 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt
     2941 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
     3001 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
     3061 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
     3121 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
     3181 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
     3241 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
     3301 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
     3361 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
     3421 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggtctcg cgccgccgtg
     3481 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
     3541 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
     3601 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     3661 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     3721 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     3781 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     3841 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     3901 tcttccattt caggtgtcgt gacaagtttg tacaaaaaag caggctgcca ccatgcagac
     3961 agcccctgtg cccatgccag acctgaagaa tgtcaaatcc aagatcggct ccactgagaa
     4021 cctgaagcac cagccgggag gcgggaaggt gcagataatt aataagaagc tggatcttag
     4081 caacgtccag tccaaatgcg gatcaaagga taatatcaaa cacgtcttgg gaggcggcag
     4141 tgtgcaaata gtctaccaac cagttgacct gagccaggtg acctcccagt gtggctcatt
     4201 aggcaacatc catcataaac caggaggtgg ccaggtggaa gtaaaatctg agaagcttga
     4261 cttcaaggac agagtccagt cgaagattgg gtccctggac aatatcaccc acgtccctgg
     4321 cggaggaaat aaaaagattg aaacccacaa gctgaccttc gaagcagccg ctaaagaggc
     4381 cgctgcgaag gaagccgcag caaaagtgag caagggagaa gagctgttca ccggggtggt
     4441 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     4501 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     4561 gctgcccgtg ccctggccca cactcgtgac caccctgtcc tggggcgtgc agtgcttcgc
     4621 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     4681 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     4741 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     4801 ggacggcaac atcctggggc acaagctgga gtacaactac tttagcgaca acgtctatat
     4861 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     4921 ggacggcggc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     4981 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     5041 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     5101 aatggatgaa ctctacaaag agggcagagg aagtctgcta acatgcggtg acgtcgagga
     5161 gaatcctgga cctcaaaccg cgcccgtccc gatgcccgat ctgaaaaacg tgaaaagcaa
     5221 gattgggagc accgagaatc tgaaacatca acccggcggg ggcaaagtcc agatcatcaa
     5281 caagaaactc gacctgtcca atgtgcaaag caagtgtggg agcaaagaca acatcaagca
     5341 tgtgctgggc ggggggagcg tccagatcgt gtatcagccc gtggatctct cccaggtcac
     5401 gagccaatgc ggcagcctgg gcaatatcca ccataagccc ggcggcgggc aagtcgaggt
     5461 gaagagcgaa aaactggatt ttaaggaccg cgtgcaaagc aaaatcggca gcctcgacaa
     5521 cattacgcat gtgcccgggg gcggcaacaa gaagatcgag acccataaac tcacctttga
     5581 ggccgccgca aaggaagctg cagccaaaga ggccgccgcc aaggtctcca aaggcgagga
     5641 agataacatg gcatctctac cagcaacaca tgagttacac atatttgggt ccatcaatgg
     5701 agtcgatttt gacatggtgg ggcagggaac aggcaatcca aatgatggtt atgaggaatt
     5761 gaatttgaag tccacaaaag gagacctcca gttctcaccc tggattctgg tccctcatat
     5821 tgggtatggc ttccaccagt atctgcccta ccctgacggg atgagccctt tccaggcagc
     5881 catggtagat ggctctggct accaagtcca tcgaacaatg cagtttgaag atggtgcctc
     5941 ccttactgtt aactaccgct atacctacga gggaagccac atcaaaggag aggcccaggt
     6001 gaaagggact ggtttccccg ctgacggtcc tgtgatgacc aactcactga ccgctgctga
     6061 ctggtgcagg tcgaaaaaga cttatcccaa tgacaaaaca attatcagta cctttaagtg
     6121 gagttacacc actggaaatg gaaagagata ccggagcact gccagaacca cgtacacctt
     6181 tgccaagcca atggcggcta actatctgaa gaaccagccg atgtacgtgt tccgtaagac
     6241 ggagcttaag cactctaaaa ccgaactcaa tttcaaagaa tggcaaaagg cctttacaga
     6301 tgttatgggc atggacgagc tgtataagta agtaaaccca gctttcttgt acaaagtggt
     6361 gataatcgaa ttccgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6421 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6481 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6541 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6601 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6661 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6721 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6781 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6841 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6901 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6961 ccccgcatcg ggaattcccg cggttcgctt taagaccaat gacttacaag gcagctgtag
     7021 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     7081 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     7141 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     7201 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     7261 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     7321 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7621 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7681 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7741 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7801 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7861 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7921 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7981 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8041 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8101 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8161 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8221 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8281 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8461 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8521 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8581 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8641 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8701 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8761 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8821 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8881 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8941 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9001 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9061 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9121 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9181 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9241 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9301 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9361 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9601 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9721 ccagtggcga taagtcgtgt cttaccgg
//
