LOCUS       sequence-410335-       12111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of VGLUT2-pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Silm K, Yang J, Marcott PF, Asensio CS, Eriksen J, Guthrie DA, 
            Newman AH, Ford CP, Edwards RH
  TITLE     Synaptic Vesicle Recycling Pathway Determines Neurotransmitter 
            Content and Release Properties.
  JOURNAL   Neuron. 2019 May 22;102(4):786-800.e5. doi: 
            10.1016/j.neuron.2019.03.031. Epub 2019 Apr 16.
  PUBMED    31003725
REFERENCE   2  (bases 1 to 12111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.03.031"; journalName: "Neuron"; date: 
            "2019-05-22- 22"; volume: "102"; issue: "4"; pages: "786-800.e5"
COMMENT     Sequence Label: FUGW-VGLUT2-pHluorin
FEATURES             Location/Qualifiers
     source          1..12111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2697..3908
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3745..3764
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4449..5159
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4476..4504)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5099..5119
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(5376..5387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    6803..7391
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6856..6876)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7274..7285)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7394..7410)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7395..7411)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7916..8096
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8122..8139)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8128..8352
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8398..8826
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8485..8504)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8695..8716
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8835..8855)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8840..9169
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9020..9155
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9217..9264
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9283..9657
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9787..9908
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9824..9843)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9878..9897
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9957..9973)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9957..9973)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9970..9992)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9981..9997
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10005..10011,10012..10029,10030..10035))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10050..10071
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10188..10205)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10359..10947)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10439..10458)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11118..11909,11910..11978))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11741..11760
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11979..12083)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatcg catgctagcg tatactgagc gatgatcgtg attgatatcc
     2641 gtcacgatca tcgctcagta tatttttctt aattaacccg tgtcggctcc agatctggcc
     2701 tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc ctcacggcga gcgctgccac
     2761 gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc cggacgctca ggacagcggc
     2821 ccgctgctca taagactcgg ccttagaacc ccagtatcag cagaaggaca ttttaggacg
     2881 ggacttgggt gactctaggg cactggtttt ctttccagag agcggaacag gcgaggaaaa
     2941 gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg ggcggtgaac gccgatgatt
     3001 atataaggac gcgccgggtg tggcacagct agttccgtcg cagccgggat ttgggtcgcg
     3061 gttcttgttt gtggatcgct gtgatcgtca cttggtgagt agcgggctgc tgggctggcc
     3121 ggggctttcg tggccgccgg gccgctcggt gggacggaag cgtgtggaga gaccgccaag
     3181 ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg ggggttgggg ggagcgcagc
     3241 aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt gtgaggcggg ctgtgaggtc
     3301 gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc aaggtcttga ggccttcgct
     3361 aatgcgggaa agctcttatt cgggtgagat gggctggggc accatctggg gaccctgacg
     3421 tgaagtttgt cactgactgg agaactcggt ttgtcgtctg ttgcgggggc ggcagttatg
     3481 gcggtgccgt tgggcagtgc acccgtacct ttgggagcgc gcgccctcgt cgtgtcgtga
     3541 cgtcacccgt tctgttggct tataatgcag ggtggggcca cctgccggta ggtgtgcggt
     3601 aggcttttct ccgtcgcagg acgcagggtt cgggcctagg gtaggctctc ctgaatcgac
     3661 aggcgccgga cctctggtga ggggagggat aagtgaggcg tcagtttctt tggtcggttt
     3721 tatgtaccta tcttcttaag tagctgaagc tccggttttg aactatgcgc tcggggttgg
     3781 cgagtgtgtt ttgtgaagtt ttttaggcac cttttgaaat gtaatcattt gggtcaatat
     3841 gtaattttca gtgttagact agtaaattgt ccgctaaatt ctggccgttt ttggcttttt
     3901 tgttagacga agcttgggct gcaggtcgac tctagaggat ccctctccaa ctcacagcct
     3961 tgctgaaaag ctcatctctg ctgagaagaa aacgttctac cttaacctat taagactatg
     4021 cgcagaactc gcctttcata gccatcacaa tttaaatctg gtaaggctgg acacgagtct
     4081 ttacaagaat ggagtcggta aaacaaagga ttttggcccc ggggaaagag gggataaaga
     4141 attttgctgg aaaatccctc ggacagatct acagggtgct ggagaagaag caggataacc
     4201 gagagaccat cgagctgaca gaggacggca agcccctgga ggtgcctgag aagaaggctc
     4261 cgctatgcga ctgtacgtgc ttcggcctgc cgcgccgcta catcatagcc atcatgagcg
     4321 gcctcggctt ctgcatctcc tttggtatcc gctgtaacct gggtgtggcc attgtggaca
     4381 tggtcaacaa cagcaccatc caccggggta ccggtagcac tagcggcgga agcggcggga
     4441 caggtggaag taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag
     4501 atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat
     4561 acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt ccttggccaa
     4621 cacttgtcac tactttaact tatggtgttc aatgcttttc aagataccca gatcatatga
     4681 aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat
     4741 ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc
     4801 ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac
     4861 acaaattgga atacaactat aacgatcacc aggtgtacat catggcagac aaacaaaaga
     4921 atggaatcaa agctaacttc aaaattagac acaacattga agatggaggc gttcaactag
     4981 cagaccatta tcaacaaaat actccaattg gcgatgggcc cgtcctttta ccagacaacc
     5041 attacctgtt tacaacttct actctttcga aagatcccaa cgaaaagaga gaccacatgg
     5101 tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaaa
     5161 gcggaggcac aggtggcagc ggcggaaccg gaggctccgg tggcaccggt ggcaaagtta
     5221 tcaaggagaa agccaagttt aactgggacc ccgagactgt ggggatgatt cacgggtcgt
     5281 tcttctgggg ctatatcatc acgcagattc cgggcggata catcgcatcg cgactggctg
     5341 ctaaccgggt ctttggggct gccatactgc ttacctctac cctcaatatg ctgatcccat
     5401 ctgcagccag agtgcattat ggatgcgtca tctttgttag aatattgcaa ggacttgtgg
     5461 agggcgtcac ctacccagcc tgtcacggga tatggagcaa gtgggcccct cctttggaga
     5521 ggagtaggtt ggctaccacc tccttctgtg gttcctatgc tggagcagtc attgcaatgc
     5581 ccctagctgg tatcctggtg cagtacactg gatggtcttc agtattttac gtatatggaa
     5641 gctttggtat ggtctggtat atgttctggc ttctggtgtc ttacgagagc cccgcaaagc
     5701 atccaaccat aacagacgaa gaacgtaggt acatagaaga gagcatcggg gagagcgcaa
     5761 atctgttagg agcaatggag aaattcaaga ccccatggag gaagtttttc acatccatgc
     5821 ccgtctatgc gataattgtt gcaaacttct gcaggagttg gactttttat ttactgctca
     5881 tcagtcaacc agcttatttc gaggaggttt ttggatttga aatcagcaag gttggcatgt
     5941 tgtctgcggt cccacacctg gtcatgacaa tcattgtgcc tatcgggggg caaattgcag
     6001 actttctaag gagcaagcaa attctttcaa caactacagt gcgaaagatc atgaactgcg
     6061 ggggttttgg catggaagcc acactgcttc tggttgttgg ctactctcat actagagggg
     6121 tggccatctc cttcttggtg cttgcagtgg gattcagtgg atttgctatc tctggtttca
     6181 atgtgaacca cttggatatt gccccgagat atgccagtat cttaatgggc atttcaaatg
     6241 gtgttggcac gctgtcggga atggtctgcc cgatcattgt tggtgcaatg acgaagaaca
     6301 agtcccgtga agaatggcag tatgtcttcc tcatcgctgc actggtccac tatggtggag
     6361 tcatatttta tgcactattt gcctcaggag agaagcaacc ttgggcagac cctgaggaaa
     6421 caagcgaaga aaagtgtggc ttcattcatg aagatgaact ggatgaagaa acgggggaca
     6481 tcactcagaa ttacataaat tacggtacca ccaaatccta cggcgccacc tcacaggaga
     6541 acggaggctg gcctaacggc tgggagaaaa aggaagaatt tgtgcaagaa agtgcgcaag
     6601 acgcgtactc ctataaggac cgagatgatt attcataacg aagctagttg ctggattcct
     6661 ttgtagtgtt tgtgattaaa ttaattgtga ttgcacaaaa tcattttaag aaatgtggtg
     6721 taaacatgta aacacatcaa ccaagcaagt cttgctgttc aaaaaataat aataatatga
     6781 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6841 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6901 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6961 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     7021 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     7081 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     7141 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
     7201 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     7261 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     7321 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     7381 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
     7441 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
     7501 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
     7561 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
     7621 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
     7681 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
     7741 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
     7801 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
     7861 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
     7921 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     7981 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8041 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
     8101 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     8161 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     8221 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     8281 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     8341 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     8401 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     8461 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     8521 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     8581 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     8641 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     8701 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     8761 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     8821 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     8881 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     8941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     9001 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     9061 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     9121 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     9181 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
     9241 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
     9301 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
     9361 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
     9421 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     9481 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
     9541 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
     9601 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
     9661 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     9721 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     9781 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     9841 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     9901 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     9961 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
    10021 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    10081 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    10141 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    10201 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    10261 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    10321 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    10381 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    10441 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    10501 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    10561 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    10621 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    10681 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    10741 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    10801 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    10861 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    10921 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    10981 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    11041 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    11101 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    11161 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    11221 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    11281 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    11341 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    11401 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    11461 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    11521 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    11581 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    11641 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    11701 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    11761 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    11821 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    11881 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    11941 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    12001 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    12061 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
