LOCUS       sequence-410340-       11964 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of VMAT2-pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11964)
  AUTHORS   Silm K, Yang J, Marcott PF, Asensio CS, Eriksen J, Guthrie DA, 
            Newman AH, Ford CP, Edwards RH
  TITLE     Synaptic Vesicle Recycling Pathway Determines Neurotransmitter 
            Content and Release Properties.
  JOURNAL   Neuron. 2019 May 22;102(4):786-800.e5. doi: 
            10.1016/j.neuron.2019.03.031. Epub 2019 Apr 16.
  PUBMED    31003725
REFERENCE   2  (bases 1 to 11964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2019.03.031"; journalName: "Neuron"; date: 
            "2019-05-22- 22"; volume: "102"; issue: "4"; pages: "786-800.e5"
COMMENT     Sequence Label: FUGW-VMAT2-pHluorin
FEATURES             Location/Qualifiers
     source          1..11964
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2605..2819
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     2740..2759
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        2868..4079
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3916..3935
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             4519..5229
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4546..4574)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     5169..5189
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    6652..7240
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6705..6725)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7123..7134)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7243..7259)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7244..7260)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7769..7949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7975..7992)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7981..8205
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8251..8679
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8338..8357)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8548..8569
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8688..8708)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8693..9022
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8873..9008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8935..8954
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9070..9117
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9136..9510
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9640..9761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9677..9696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9731..9750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9810..9826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9810..9826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9823..9845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9834..9850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9858..9864,9865..9882,9883..9888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9903..9924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10041..10058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10212..10800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10292..10311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10971..11762,11763..11831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(11832..11936)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaattc tagcgaacgc tgacgtcatc aacccgctcc aaggaatcgc
     2641 gggcccagtg tcactaggcg ggaacaccca gcgcgcgtgc gccctggcag gaagatggct
     2701 gtgagggaca ggggagtggc gccctgcaat atttgcatgt cgctatgtgt tctgggaaat
     2761 caccataaac gtgaaatgtc tttggatttg ggaatcttat aagttctgta tgagaccaca
     2821 gatccccctc gagtctagag ttaacttaat taacccaccc aagatctggc ctccgcgccg
     2881 ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca cgtcagacga
     2941 agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg cccgctgctc
     3001 ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac gggacttggg
     3061 tgactctagg gcactggttt tctttccaga gagcgaaaca ggcgaggaaa agtagtccct
     3121 tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat tatataagga
     3181 cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc ggttcttgtt
     3241 tgtggatcgc tgtgatcgtc acttggtgag tagcgggctg ctgggctggc cggggctttc
     3301 gtggccgccg ggccgctcgg tgggacggaa gcgtgtggag agaccgccaa gggctgtagt
     3361 ctgggtccgc gagcaaggtt gccctgaact gggggttggg gggagcgcag caaaatggcg
     3421 gctgttcccg agtcttgaat ggaagacgct tgtgaggcgg gctgtgaggt cgttgaaaca
     3481 aggtgggggg catggtgggc ggcaagaacc caaggtcttg aggccttcgc taatgcggga
     3541 aagctcttac tcgggtgaga tgggctgggg caccatctgg ggaccctgac gtgaagtttg
     3601 tcactgactg gagaactcgg tttgtcgtct gttgcggggg cggcagttat ggcggtgccg
     3661 ttgggcagtg cacccgtacc tttgggagcg cgcgccctcg tcgtgtcgtg acgtcacccg
     3721 ttctgttggc ttataatgca gggtggggcc acctgccggt aggtgtgcgg taggcttttc
     3781 tccgtcgcag gacgcagggt tcgggcctag ggtaggctct cctgaatcga caggcgccgg
     3841 acctctggtg aggggaggga taagtgaggc gtcagtttct ttggtcggtt ttatgtacct
     3901 atcttcttaa gtagctgaag ctccggtttt gaactatgcg ctcggggttg gcgagtgtgt
     3961 tttgtgaagt tttttaggca ccttttgaaa tgtaatcatt tgggtcaata tgtaattttc
     4021 agtgttagac tagtaaattg tccgctaaat tctggccgtt tttggctttt ttgttagacg
     4081 aagcttggta ccgagcggat ccgaattcgg gcgcacggac agagacccag gctgtgtggc
     4141 gctataaccg cgcagtcaca ggcgagccag agcagagcca tggccctgag cgatctggtg
     4201 ctgctgcgat ggctgcggga cagccgccac tcgcgcaaac tgatcctgtt catcgtgttc
     4261 cttgcgctgc tgctggacaa catgctgctc accgtcgtgg ttcccatcat ccccagctat
     4321 ctgtacagca ttaagcatga gaaaaactct acggaaatcc agaccaccag accagagctc
     4381 gtggtctcca cctccgaaag catcttctct tactataaca actctactgt gttgatcacc
     4441 gggaatgcca ctgggactct tccaggaggc agcggcggga ccggtagcac tagcggcgga
     4501 agcggcggga caggtggaag taaaggagaa gaacttttca ctggagttgt cccaattctt
     4561 gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga gggtgaaggt
     4621 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     4681 ccttggccaa cacttgtcac tactttaact tatggtgttc aatgcttttc aagataccca
     4741 gatcatatga aacggcatga ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa
     4801 agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt caagtttgaa
     4861 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
     4921 attcttggac acaaattgga atacaactat aacgatcacc aggtgtacat catggcagac
     4981 aaacaaaaga atggaatcaa agctaacttc aaaattagac acaacattga agatggaggc
     5041 gttcaactag cagaccatta tcaacaaaat actccaattg gcgatgggcc cgtcctttta
     5101 ccagacaacc attacctgtt tacaacttct actctttcga aagatcccaa cgaaaagaga
     5161 gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg catggatgaa
     5221 ctatacaaaa gcggaggcac aggtggcagc ggcggaaccg gaggctccgg tggcaccggt
     5281 ggaagcggag ggaccggagg gcagtcacac aaggctacca gcacacagca cactgtggct
     5341 aacaccactg tcccttcgga ctgtcccagt gaagacagag atcttctgaa tgagaatgtg
     5401 caagttgggc tgctgtttgc ctccaaagcc actgtccagc tcctcactaa cccattcata
     5461 ggacttctga ccaacagaat tggctatcca attcccatgt ttgccggctt ctgcatcatg
     5521 tttatctcaa cagttatgtt tgccttctcc agcagctatg ccttcctgct gatcgccagg
     5581 tcccttcagg gaattggctc ctcctgctca tccgtggctg ggatgggtat gctggccagc
     5641 gtgtacacag atgatgagga gagggggaac gccatgggca ttgctttggg tggcctggcc
     5701 atgggagtct tagtgggacc ccccttcggg agtgtgctct atgagtttgt ggggaagaca
     5761 gctcccttcc tggtgctagc tgccttggtg ctcttggatg gggctattca gctctttgtg
     5821 ctccagccgt cccgagtaca gccagagagt cagaagggga cacctctaac gaccttgctg
     5881 aaggatccat acatcctcat cgctgcaggc tccatctgct ttgcaaacat ggggatagcc
     5941 atgctggagc ccgccctgcc catctggatg atggagacca tgtgttcccg aaagtggcag
     6001 ctgggcgttg ctttcctccc ggcgagcatc tcttatctca ttggaaccaa tatttttggg
     6061 atacttgcac acaaaatggg aaggtggcta tgtgctcttc tgggaatggt aattgttgga
     6121 atcagcattt tatgcatccc ctttgcaaaa aatatctatg gactcatcgc tcccaacttt
     6181 ggagttggtt ttgcaattgg gatggtggac tcctctatga tgcctatcat gggctacctg
     6241 gtcgacctgc ggcatgtgtc tgtctatggg agtgtttatg ccattgcaga cgtggccttt
     6301 tgtatgggct atgctatcgg tccctctgct ggtggtgcca tcgcaaaggc aattggcttt
     6361 ccttggctta tgacaattat tgggataatt gatatcgctt ttgctccact ctgctttttc
     6421 cttcgaagtc cacctgctaa ggaggaaaaa atggctatcc tcatggacca caactgtccc
     6481 attaaaacaa agatgtacac tcagaataat gtccagtcat atcccatcgg tgatgatgaa
     6541 gaatctgaaa gtgactgaga ccctctaacg tcgcccgaat tcgcaaccgg tgcagccttc
     6601 gaagcagcta gcgactctag tccctcccaa ttcgatatca agcttatcga taatcaacct
     6661 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     6721 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     6781 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     6841 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     6901 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     6961 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     7021 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     7081 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     7141 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     7201 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     7261 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     7321 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     7381 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7441 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     7501 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     7561 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     7621 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     7681 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     7741 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     7801 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     7861 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     7921 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     7981 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8041 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8101 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     8161 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     8221 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     8281 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     8341 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     8401 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     8461 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     8521 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     8581 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     8641 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     8701 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     8761 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8821 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     8881 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8941 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9001 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     9061 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     9121 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
     9181 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
     9241 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
     9301 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
     9361 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
     9421 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
     9481 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
     9541 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     9601 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
     9661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     9721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
     9781 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9841 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9901 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     9961 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10021 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    10081 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    10141 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    10201 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    10261 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    10321 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    10381 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    10441 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    10501 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    10561 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    10621 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    10681 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    10741 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    10801 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    10861 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    10921 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    10981 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    11041 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    11101 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    11161 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    11221 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    11281 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    11341 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    11401 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    11461 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    11521 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    11581 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    11641 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    11701 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    11761 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    11821 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    11881 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    11941 tttccccgaa aagtgccacc tgac
//
