LOCUS       sequence-410343-       12011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of VGLUT1-pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12011)
  TITLE     Edwards Lab pHluorin Plasmids
REFERENCE   2  (bases 1 to 12011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUGW-VGLUT1-pHluorin
FEATURES             Location/Qualifiers
     source          1..12011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3833
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3670..3689
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3977..3986
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4331..5041
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(4358..4386)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     4981..5001
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             complement(6069..6080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    6703..7291
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6756..6776)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7174..7185)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7294..7310)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7295..7311)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7816..7996
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8022..8039)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8028..8252
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8298..8726
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8385..8404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8595..8616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8735..8755)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8740..9069
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8920..9055
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8982..9001
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9117..9164
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9183..9557
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9687..9808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9724..9743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9778..9797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9857..9873)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9857..9873)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9870..9892)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9881..9897
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9905..9911,9912..9929,9930..9935))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9950..9971
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10088..10105)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10259..10847)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10339..10358)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11018..11809,11810..11878))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11641..11660
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11879..11983)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgactctag aattcggcac gagcggagct gcggggccgg gccgggccgg
     3901 ggcggacccc gggatcccgg acgcggccgc ccgggcccgc gggcgggggg attggcaggg
     3961 gacccgcgtg ggcacagcca ccatggagtt ccggcaggag gagtttcgga agctggcggg
     4021 gcgcgccctg gggaggctgc accggttact ggagaagcgg caggaaggcg cggagacatt
     4081 ggagctgagc gccgacgggc gcccagtgac cacacacacg cgggacccgc cggtggtgga
     4141 ctgcacttgc tttggcctcc ctcgccgcta catcatcgcg atcatgagcg gtctgggttt
     4201 ctgcatcagc tttggcatcc gctgcaacct gggcgtggcc atcgtatcca tggtcaacaa
     4261 cagtacaacc caccgtgggg gccacgtggt gtccggtagc actagcggcg gaagcggcgg
     4321 gacaggtgga agtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
     4381 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
     4441 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccttggcc
     4501 aacacttgtc actactttaa cttatggtgt tcaatgcttt tcaagatacc cagatcatat
     4561 gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
     4621 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
     4681 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
     4741 acacaaattg gaatacaact ataacgatca ccaggtgtac atcatggcag acaaacaaaa
     4801 gaatggaatc aaagctaact tcaaaattag acacaacatt gaagatggag gcgttcaact
     4861 agcagaccat tatcaacaaa atactccaat tggcgatggg cccgtccttt taccagacaa
     4921 ccattacctg tttacaactt ctactctttc gaaagatccc aacgaaaaga gagaccacat
     4981 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa
     5041 aagcggaggc acaggtggca gcggcggaac cggaggctcc ggtggcaccg gagtgcagaa
     5101 agcccagttc aactgggatc cagagactgt cggcctcata catggctcct ttttctgggg
     5161 gtacattgtc actcagattc ctggaggatt tatctgccaa aaattcgcag ccaacagggt
     5221 ctttggcttt gccattgtgg ctacctccac cctaaatatg ttgatccctt cagcagcccg
     5281 tgttcactat ggctgtgtca tcttcgtgag gatccttcag ggattggtgg agggggtcac
     5341 ataccctgct tgccatggca tctggagcaa atgggcccct cccttagaac ggagtcggct
     5401 ggcgacgaca gccttttgcg gttcctatgc cggggcagtg gttgccatgc ctctggctgg
     5461 ggtcctggta cagtattcag gatggagttc tgtcttctat gtctatggca gcttcgggat
     5521 cttttggtac ctgttctggt tgcttgtctc ctacgagtca cctgcactac accccagcat
     5581 ctccgaggag gagcgcaaat acattgagga tgccatcgga gaaagcgcca agctcatgaa
     5641 ccctgttacg aagtttaaca caccctggag gcgcttcttt acctccatgc cggtctatgc
     5701 catcattgtc gccaactttt gccgcagctg gactttctac ctgctcctca tctcccagcc
     5761 cgcctacttt gaagaagtgt tcggctttga gatcagcaag gtgggactgg tgtcggcact
     5821 gcctcacctt gtcatgacta tcatcgtacc catcggaggc cagatcgccg acttcctgcg
     5881 cagtcgtcat ataatgtcca cgaccaatgt gcgaaagctg atgaactgcg ggggtttcgg
     5941 gatggaagct acgctgctgc tggtggtcgg atactcacac tccaagggcg tggccatctc
     6001 cttcctggtc ctggctgtgg gcttcagtgg ctttgctatc tctgggttta acgtgaacca
     6061 cttggacatc gcccctcgat atgccagcat cttgatgggc atttccaatg gcgtgggcac
     6121 actgtctggg atggtgtgcc ccatcatcgt gggtgcaatg accaagcaca agacgcggga
     6181 ggagtggcag tacgtgttcc tcatagcctc cctggtgcac tatggaggtg tcatcttcta
     6241 tggggtcttt gcttcgggag agaaacagcc gtgggcagag ccggaggaga tgagcgagga
     6301 gaagtgtggc tttgttggcc acgaccagct ggctggcagt gacgaaagtg aaatggaaga
     6361 cgaggttgag cccccggggg caccccccgc acctccgcct tcctacgggg ccacacacag
     6421 cacagttcag cctccaaggc ccccaccccc tgtccgggac tactgaccac gtgcctccca
     6481 ctggtgggca gtttccagga cctccactcg atacacctct agcctaaacg gcagtgtcga
     6541 ggaaccccac tcctctcctg cctcaggctt aagatgcaag tcttcccttg tgcccagtgc
     6601 tgtccgacca gccctctctc cttctcaact gcctcttgca ggggtgaagc tgcacactag
     6661 cagtttcaag ctcgtgccga attcgatatc aagcttatcg ataatcaacc tctggattac
     6721 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6781 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6841 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6901 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6961 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     7021 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     7081 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     7141 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     7201 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     7261 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agacctagaa
     7321 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     7381 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     7441 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     7501 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     7561 ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata tccactgacc
     7621 tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga agccaatgaa
     7681 ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     7741 gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc ccgagagctg
     7801 catccggact gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     7861 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     7921 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     7981 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     8041 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     8101 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     8161 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     8221 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     8281 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     8341 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8461 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8521 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8581 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8641 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8701 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     8761 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8821 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8881 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8941 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9001 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9061 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     9121 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     9181 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     9241 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     9301 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     9361 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     9421 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     9481 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     9541 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     9601 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     9661 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     9721 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     9781 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     9841 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     9901 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     9961 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
    10021 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
    10081 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
    10141 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
    10201 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
    10261 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
    10321 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
    10381 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
    10441 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
    10501 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
    10561 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
    10621 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
    10681 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
    10741 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
    10801 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
    10861 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
    10921 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
    10981 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
    11041 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
    11101 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
    11161 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
    11221 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
    11281 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
    11341 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
    11401 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
    11461 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
    11521 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
    11581 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
    11641 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
    11701 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
    11761 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
    11821 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
    11881 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
    11941 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
    12001 tgccacctga c
//
