LOCUS       sequence-410346-       11621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of VGAT-pHluorin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11621)
  AUTHORS   Jain S, Yee AG, Maas J, Gierok S, Xu H, Stansil J, Eriksen J, Nelson
            A, Silm K, Ford CP, Edwards RH
  TITLE     Adaptor Protein-3 Produces Synaptic Vesicles that Release Phasic 
            Dopamine
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 11621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: FUGW-VGAT-pHluorin
FEATURES             Location/Qualifiers
     source          1..11621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2622..3833
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3670..3689
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      3928..3937
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5518..6228
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5545..5573)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6168..6188
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             6243..6269
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6313..6901
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6366..6386)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6784..6795)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6904..6920)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6905..6921)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7426..7606
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7632..7649)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7638..7862
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7908..8336
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7995..8014)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8205..8226
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8345..8365)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8350..8679
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8530..8665
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8592..8611
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8727..8774
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8793..9167
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9297..9418
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9334..9353)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9388..9407
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9467..9483)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9467..9483)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9480..9502)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9491..9507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9515..9521,9522..9539,9540..9545))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9560..9581
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9698..9715)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9869..10457)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9949..9968)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10628..11419,11420..11488))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11251..11270
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11489..11593)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
     2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
     3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
     3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
     3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
     3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
     3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
     3841 gggctgcagg tcgactctag acccttgctg ccttgacgcg cgcccgccgc gtccccagac
     3901 ccttctgtcc ttttctcccg ccccgccgcc gccatggcca ccctgctccg cagcaagctg
     3961 accaacgtgg ccacctctgt gtccaacaag tcccaggcca aggtgagcgg catgttcgcc
     4021 aggatggggt ttcaggcggc cacggatgag gaggcggtgg gcttcgcgca ctgcgacgat
     4081 ctcgactttg agcaccgcca gggcctgcag atggacatcc tgaaatcgga aggcgagccc
     4141 tgcggggacg agggcgcaga acctcccgtc gagggagaca ttcattatca gcgcggcggc
     4201 gctcccctgc caccctcggg ctccaaggac caggccgtgg gagctggtgg ggagttcggg
     4261 ggtcacgaca aacccaagat cacggcgtgg gaagcgggct ggaacgtgac aaacgccatt
     4321 cagggcatgt tcgtgctggg tctaccctac gccatcctcc acggcggcta cctggggttg
     4381 ttcctcatca tcttcgccgc ggtggtgtgc tgctacaccg gcaagatcct catcgcgtgc
     4441 ctgtacgagg agaacgaaga tggtgaggtg gtgcgcgtga gggactcgta tgtggccata
     4501 gctaacgcgt gctgcgctcc tcgattcccc acgctgggcg gccgcgtggt caatgtggcc
     4561 cagatcatcg agctggtgat gacgtgtatc ttgtacgtag tggtgagcgg caacctcatg
     4621 tacaacagtt tcccggggct gcccgtgtcg cagaagtcct ggtccatcat agccacggcg
     4681 gtgctgctgc cctgcgcctt cctgaagaat ctcaaggccg tgtccaagtt cagtctgctg
     4741 tgcacgctgg cccacttcgt catcaacatc ctggtcatcg cctactgtct ctcgcgcgcg
     4801 cgtgactggg cctgggagaa ggtgaagttc tacatcgacg tcaagaagtt tcctatctcc
     4861 atcggcatca tcgtgttcag ctacacgtcg cagatcttcc tgccctcgct cgaaggcaac
     4921 atgcagcagc ccagcgaatt ccactgcatg atgaactgga cacacatcgc cgcctgcgtg
     4981 ctcaagggtc tcttcgcgct cgtcgcctac ctcacctggg ccgacgagac caaggaagtc
     5041 atcacggata acctgcccgg ttccatccgc gccgtggtca acatcttcct ggtggccaag
     5101 gcgctgctgt cctacccgtt gcccttcttc gcggccgtcg aagtgctgga gaagtctctc
     5161 ttccaggaag gcagtcgtgc cttcttcccc gcctgctacg gtggcgacgg tcgccttaag
     5221 tcctgggggc tgacgctgcg ctgcgcgctg gtggtcttca cgctgctcat ggccatctac
     5281 gtgccacact tcgcgctgct catgggcctc acgggcagcc tcacgggagc cggcctctgc
     5341 ttcctgctgc ccagcctctt ccacttgcgt cttctctggc gcaagctgct gtggcaccag
     5401 gtcttcttcg atgtggccat cttcgtcatc ggcggcatct gcagcgtgtc cggcttcgtg
     5461 cattcactcg agggcctcat cgaggcctac cgaaccaacg cagaggacac cggtggaagt
     5521 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
     5581 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt
     5641 acccttaaat ttatttgcac tactggaaaa ctacctgttc cttggccaac acttgtcact
     5701 actttaactt atggtgttca atgcttttca agatacccag atcatatgaa acggcatgac
     5761 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
     5821 gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga
     5881 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
     5941 tacaactata acgatcacca ggtgtacatc atggcagaca aacaaaagaa tggaatcaaa
     6001 gctaacttca aaattagaca caacattgaa gatggaggcg ttcaactagc agaccattat
     6061 caacaaaata ctccaattgg cgatgggccc gtccttttac cagacaacca ttacctgttt
     6121 acaacttcta ctctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag
     6181 tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaaac cggttaggcg
     6241 gttacccgta cgacgtgccg gactacgcct agctagcgga tccgttaaca attcgatatc
     6301 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     6361 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     6421 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     6481 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     6541 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     6601 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     6661 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     6721 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     6781 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     6841 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     6901 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     6961 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     7021 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     7081 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     7141 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     7201 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     7261 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     7321 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     7381 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     7441 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     7501 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     7561 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     7621 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     7681 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     7741 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     7801 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     7861 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     7921 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     7981 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     8041 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     8101 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     8161 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     8221 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     8281 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     8341 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     8401 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     8461 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     8521 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     8581 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     8641 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     8701 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
     8761 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     8821 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     8881 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     8941 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     9001 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     9061 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     9121 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
     9181 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
     9241 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
     9301 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9361 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9421 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
     9481 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     9541 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     9601 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     9661 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     9721 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     9781 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     9841 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     9901 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     9961 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    10021 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    10081 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    10141 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    10201 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    10261 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    10321 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    10381 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    10441 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    10501 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    10561 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    10621 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    10681 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    10741 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    10801 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    10861 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    10921 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    10981 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    11041 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    11101 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    11161 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    11221 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    11281 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    11341 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    11401 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    11461 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    11521 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    11581 taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
