LOCUS       sequence-410352-        9790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the wild type form of human JAK3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9790)
  AUTHORS   Omari SA, Kosasih HJ, Chung T, de Bock CE
  TITLE     In vitro and in vivo modelling of mutant JAK3/STAT5 signaling in 
            leukemia.
  JOURNAL   Heliyon. 2023 Nov 8;9(11):e22085. doi: 
            10.1016/j.heliyon.2023.e22085. eCollection 2023 Nov.
  PUBMED    38053908
REFERENCE   2  (bases 1 to 9790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Heliyon."; 
            date: "2023-11-8"; volume: "9(11):e22085. doi"; pages: " 
            10.1016/j.heliyon.2023.e22085. eCollection 2023 Nov"
COMMENT     Sequence Label: MSCV_JAK3_IRES_BFP
FEATURES             Location/Qualifiers
     source          1..9790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..157
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             357..873
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    937..1278
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1345..1761
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1691..1713
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1729..1745
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    5192..5759
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    5209..5761
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5353..5370)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5580..5599
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             5773..6474
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             6541..7055
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    7217..7233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7241..7247,7248..7265,7266..7271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7286..7307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7424..7441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7595..8183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7675..7694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8354..9145,9146..9214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8977..8996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9215..9319)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9387..9405
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9443..9465)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9565..9584
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     join(9778..9790,1..10)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ttgtaaaacg acggcgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
       61 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
      121 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
      181 ccggtgatgc cggccacgat gcgtccggcg tagaggcgat tagtccaatt tgttaaagac
      241 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagcctata
      301 gagtacgagc catagataaa ataaaagatt ttatttagtc tccagaaaaa ggggggaatg
      361 aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg
      421 aaaatacata actgagaata gagaagttca gatcaaggtt aggaacagag agacagcaga
      481 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
      541 agatggtccc cagatgcggt cccgccctca gcagtttcta gagaaccatc agatgtttcc
      601 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
      661 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
      721 cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtat tcccaataaa
      781 gcctcttgct gtttgcatcc gaatcgtgga ctcgctgatc cttgggaggg tctcctcaga
      841 ttgattgact gcccacctcg ggggtctttc atttggaggt tccaccgaga tttggagacc
      901 cctgcccagg gaccaccgac ccccccgccg ggaggtaagc tggccagcgg tcgtttcgtg
      961 tctgtctctg tctttgtgcg tgtttgtgcc ggcatctaat gtttgcgcct gcgtctgtac
     1021 tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga gttctgaaca
     1081 cccggccgca accctgggag acgtcccagg gactttgggg gccgtttttg tggcccgacc
     1141 tgaggaaggg agtcgatgtg gaatccgacc ccgtcaggat atgtggttct ggtaggagac
     1201 gagaacctaa aacagttccc gcctccgtct gaatttttgc tttcggtttg gaaccgaagc
     1261 cgcgcgtctt gtctgctgca gcgctgcagc atcgttctgt gttgtctctg tctgactgtg
     1321 tttctgtatt tgtctgaaaa ttagggccag actgttacca ctcccttaag tttgacctta
     1381 ggtcactgga aagatgtcga gcggatcgct cacaaccagt cggtagatgt caagaagaga
     1441 cgttgggtta ccttctgctc tgcagaatgg ccaaccttta acgtcggatg gccgcgagac
     1501 ggcaccttta accgagacct catcacccag gttaagatca aggtcttttc acctggcccg
     1561 catggacacc cagaccaggt cccctacatc gtgacctggg aagccttggc ttttgacccc
     1621 cctccctggg tcaagccctt tgtacaccct aagcctccgc ctcctcttcc tccatccgcc
     1681 ccgtctctcc cccttgaacc tcctcgttcg accccgcctc gatcctccct ttatccagcc
     1741 ctcactcctt ctctaggcgc cggaattaga tctctcgagc cagggtccct gcccgctagg
     1801 caagttgcac tcatggcacc tccaagtgaa gagacgcccc tgatccctca gcgttcatgc
     1861 agcctcttgt ccacggaggc tggtgccctg catgtgctgc tgcccgctcg gggccccggg
     1921 cccccccagc gcctatcttt ctcctttggg gaccacttgg ctgaggacct gtgcgtgcag
     1981 gctgccaagg ccagcggcat cctgcctgtg taccactccc tctttgctct ggccacggag
     2041 gacctgtcct gctggttccc cccgagccac atcttctccg tggaggatgc cagcacccaa
     2101 gtcctgctgt acaggattcg cttttacttc cccaattggt ttgggctgga gaagtgccac
     2161 cgcttcgggc tacgcaagga tttggccagt gctatccttg acctgccagt cctggagcac
     2221 ctctttgccc agcaccgcag tgacctggtg agtgggcgcc tccccgtggg cctcagtctc
     2281 aaggagcagg gtgagtgtct cagcctggcc gtgttggacc tggcccggat ggcgcgagag
     2341 caggcccagc ggccgggaga gctgctgaag actgtcagct acaaggcctg cctaccccca
     2401 agcctgcgcg acctgatcca gggcctgagc ttcgtgacgc ggaggcgtat tcggaggacg
     2461 gtgcgcagag ccctgcgccg cgtggccgcc tgccaggcag accggcactc gctcatggcc
     2521 aagtacatca tggacctgga gcggctggat ccagccgggg ccgccgagac cttccacgtg
     2581 ggcctccctg gggcccttgg tggccacgac gggctggggc tgctccgcgt ggctggtgac
     2641 ggcggcatcg cctggaccca gggagaacag gaggtcctcc agcccttctg cgactttcca
     2701 gaaatcgtag acattagcat caagcaggcc ccgcgcgttg gcccggccgg agagcaccgc
     2761 ctggtcactg ttaccaggac agacaaccag attttagagg ccgagttccc agggctgccc
     2821 gaggctctgt cgttcgtggc gctcgtggac ggctacttcc ggctgaccac ggactcccag
     2881 cacttcttct gcaaggaggt ggcaccgccg aggctgctgg aggaagtggc cgagcagtgc
     2941 cacggcccca tcactctgga ctttgccatc aacaagctca agactggggg ctcacgtcct
     3001 ggctcctatg ttctccgccg cagcccccag gactttgaca gcttcctcct cactgtctgt
     3061 gtccagaacc cccttggtcc tgattataag ggctgcctca tccggcgcag ccccacagga
     3121 accttccttc tggttggcct cagccgaccc cacagcagtc ttcgagagct cctggcaacc
     3181 tgctgggatg gggggctgca cgtagatggg gtggcagtga ccctcacttc ctgctgtatc
     3241 cccagaccca aagaaaagtc caacctgatc gtggtccaga gaggtcacag cccacccaca
     3301 tcatccttgg ttcagcccca atcccaatac cagctgagtc agatgacatt tcacaagatc
     3361 cctgctgaca gcctggagtg gcatgagaac ctgggccatg ggtccttcac caagatttac
     3421 cggggctgtc gccatgaggt ggtggatggg gaggcccgaa agacagaggt gctgctgaag
     3481 gtcatggatg ccaagcacaa gaactgcatg gagtcattcc tggaagcagc gagcttgatg
     3541 agccaagtgt cgtaccggca tctcgtgctg ctccacggcg tgtgcatggc tggagacagc
     3601 accatggtgc aggaatttgt acacctgggg gccatagaca tgtatctgcg aaaacgtggc
     3661 cacctggtgc cagccagctg gaagctgcag gtggtcaaac agctggccta cgccctcaac
     3721 tatctggagg acaaaggcct gccccatggc aatgtctctg cccggaaggt gctcctggct
     3781 cgggaggggg ctgatgggag cccgcccttc atcaagctga gtgaccctgg ggtcagcccc
     3841 gctgtgttaa gcctggagat gctcaccgac aggatcccct gggtggcccc cgagtgtctc
     3901 cgggaggcgc agacacttag cttggaagct gacaagtggg gcttcggcgc cacggtctgg
     3961 gaagtgttta gtggcgtcac catgcccatc agtgccctgg atcctgctaa gaaactccaa
     4021 ttttatgagg accggcagca gctgccggcc cccaagtgga cagagctggc cctgctgatt
     4081 caacagtgca tggcctatga gccggtccag aggccctcct tccgagccgt cattcgtgac
     4141 ctcaatagcc tcatctcttc agactatgag ctcctctcag accccacacc tggtgccctg
     4201 gcacctcgtg atgggctgtg gaatggtgcc cagctctatg cctgccaaga ccccacgatc
     4261 ttcgaggaga gacacctcaa gtacatctca cagctgggca agggcaactt tggcagcgtg
     4321 gagctgtgcc gctatgaccc gctaggcgac aatacaggtg ccctggtggc cgtgaaacag
     4381 ctgcagcaca gcgggccaga ccagcagagg gactttcagc gggagattca gatcctcaaa
     4441 gcactgcaca gtgatttcat tgtcaagtat cgtggtgtca gctatggccc gggccgccag
     4501 agcctgcggc tggtcatgga gtacctgccc agcggctgct tgcgcgactt cctgcagcgg
     4561 caccgcgcgc gcctcgatgc cagccgcctc cttctctatt cctcgcaaat ctgcaagggc
     4621 atggagtacc tgggctcccg ccgctgcgtg caccgcgacc tggccgcccg aaacatcctc
     4681 gtggagagcg aggcacacgt caagatcgct gacttcggcc tagctaagct gctgccgctt
     4741 gacaaagact actacgtggt ccgcgagcca ggccagagcc ccattttctg gtatgccccc
     4801 gaatccctct cggacaacat cttctctcgc cagtcagacg tctggagctt cggggtcgtc
     4861 ctgtacgagc tcttcaccta ctgcgacaaa agctgcagcc cctcggccga gttcctgcgg
     4921 atgatgggat gtgagcggga tgtccccgcc ctctgccgcc tcttggaact gctggaggag
     4981 ggccagaggc tgccggcgcc tcctgcctgc cctgctgagg ttcacgagct catgaagctg
     5041 tgctgggccc ctagcccaca ggaccggcca tcattcagcg ccctgggccc ccagctggac
     5101 atgctgtgga gcggaagccg ggggtgtgag actcatgcct tcactgctca cccagagggc
     5161 aaacaccact ccctgtcctt ttcataggaa ttccgccccc cccccctaac gttactggcc
     5221 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     5281 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     5341 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     5401 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     5461 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     5521 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     5581 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     5641 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     5701 aacgtctagg ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat
     5761 atggccacaa ccatgagcga gctgattaag gagaacatgc acatgaagct gtacatggag
     5821 ggcaccgtgg acaaccatca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
     5881 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
     5941 atcctggcta ctagcttcct ctacggcagc aagaccttca tcaaccacac ccagggcatc
     6001 cccgacttct tcaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
     6061 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
     6121 tacaacgtca agatcagagg ggtgaacttc acatccaacg gccctgtgat gcagaagaaa
     6181 acactcggct gggaggcctt caccgagacg ctgtaccccg ctgacggcgg cctggaaggc
     6241 agaaacgaca tggccctgaa gctcgtgggc gggagccatc tgatcgcaaa cgccaagacc
     6301 acatatagat ccaagaaacc cgctaagaac ctcaagatgc ctggcgtcta ctatgtggac
     6361 tacagactgg aaagaatcaa ggaggccaac aacgagacct acgtcgagca gcacgaggtg
     6421 gcagtggcca gatactgcga cctccctagc aaactggggc acaagcttaa ttaagtcgac
     6481 ctgcagccaa gcttatcgat aaaataaaag attttattta gtctccagaa aaagggggga
     6541 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttgcaaggca
     6601 tggaaaatac ataactgaga atagagaagt tcagatcaag gttaggaaca gagagacagc
     6661 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     6721 aacagatggt ccccagatgc ggtcccgccc tcagcagttt ctagagaacc atcagatgtt
     6781 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     6841 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     6901 cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc cgggtacccg tgtatccaat
     6961 aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct
     7021 gagtgattga ctacccgtca gcgggggtct ttcatgggta acagtttctt gaagttggag
     7081 aacaacattc tgagggtagg agtcgaatat taagtaatcc tgactcaatt agccactgtt
     7141 ttgaatccac atactccaat actcctgaaa tagttcatta tggacagcgc agaaagagct
     7201 ggggagaatt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     7261 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     7321 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     7381 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     7441 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     7501 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     7561 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     7621 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     7681 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     7741 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     7801 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     7861 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     7921 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7981 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     8041 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     8101 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     8161 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     8221 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     8281 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     8341 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     8401 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     8461 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     8521 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     8581 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     8641 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     8701 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     8761 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     8821 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     8881 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     8941 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9001 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     9061 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     9121 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     9181 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     9241 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     9301 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     9361 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     9421 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     9481 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     9541 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     9601 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     9661 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9721 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9781 agtcacgacg
//
