LOCUS       sequence-410361-        5749 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPC5 protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5749)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 5749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GPC5A-c001
FEATURES             Location/Qualifiers
     source          1..5749
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        492..871
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        902..1006
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1007..1075,1076..1867)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1225..1244)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2038..2626
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2527..2546
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2780..2797
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2926..2947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2962..2967,2968..2985,2986..2992)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3000..3016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3005..3027
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3024..3040
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3024..3040
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3383..3402
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    3401..3456
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     3502..3521
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(3633..3650)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3663..3707)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     regulatory      5393..5402
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(5457..5476)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     complement(5538..5560)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
ORIGIN
        1 ccgcacagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc
       61 aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccgggggccg ccgcgcccct
      121 cccccsagcc cyccccsgcc cgmggcggcc ccsccccscc csncmccccc cccccccccc
      181 cccccccccc cccccccscc mccccccccc cccccccccc cccccccccc cccccccccc
      241 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc
      301 cccccccccc cccccccccc cccccccccc cccccccccc cccccccccc ccccccccnn
      361 vgsvcscccc cccccccccc ccccgccccc atcgctgcac aaaataatta aaaaataaat
      421 aaatacaaaa ttgggggtgg ggaggggggg gagatgggga gagtgaagca gaacgtgggg
      481 ctcacctcga ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
      541 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
      601 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
      661 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
      721 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
      781 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
      841 tactattaat aactagtcaa taatcaatgt cgacccaggt ggcacttttc ggggaaatgt
      901 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      961 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     1021 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     1081 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     1141 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     1201 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     1261 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     1321 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     1381 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1441 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1501 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1561 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1621 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1681 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1741 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1801 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1861 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1921 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1981 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2041 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2101 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2161 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2221 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2281 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2341 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2401 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2461 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2521 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2581 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2641 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2701 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2761 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2821 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agcggatcga tccgctggca
     2881 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     2941 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     3001 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgg
     3061 gctgcaggtc gagggatctc cataagagaa gagggacagc tatgactggg agtagtcagg
     3121 agaggaggaa aaatctggct agtaaaacat gtaaggaaaa ttttagggat gttaaagaaa
     3181 aaaataacac aaaacaaaat ataaaaaaaa tctaacctca agtcaaggct tttctatgga
     3241 ataaggaatg gacagcaggg ggctgtttca tatactgatg acctctttat agccaacctt
     3301 tgttcatggc agccagcata tgggcatatg ttgccaaact ctaaaccaaa tactcattct
     3361 gatgttttaa atgatttgcc ctcccatatg tccttccgag tgagagacac aaaaaattcc
     3421 aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga tgctcaaggg
     3481 gcttcatgat gtccccataa tttttggcag agggaaaaag atctcagtgg tatttgtgag
     3541 ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtga
     3601 attatcgcga tactagtctc gagtgatcat tagtgatggt gatggtggtg cttggtacgt
     3661 ccttcatgcc attcaatctt ctgagcttca aagatatcat tcagaccgga accaccggaa
     3721 cctccggtac ctgctcctgt tgtgtctaaa gtactgccag tgtctgtttg atggatttgc
     3781 tttacatcac tgaagttcat atcatctggc atccagtctg tgatcttcag tgtcctcttg
     3841 acttctccac ttcctgatcc cccgcaacca tcttcatcat cacagtcccc actgacttgt
     3901 tcaaccatgc ctccaccact gcccagctga agaagttccc acttgtcagg tttgggtgat
     3961 ctaccctgta acaactgaac aacatgcttc agtttatcaa taatctgatt tatcacagga
     4021 tcaattcctt tgactttgac ttcaggattt ccagactggg ctttgattcc atttccaacc
     4081 acacgctgag tataactttt tactatatct tctccattcc agcagggaag tccatctgca
     4141 gcagctaatt cattagcaca aagctgatca gctagacctc catagaatga cctgtacagt
     4201 cgaaggctgt tgataaattc ttttcttctg ttggcaagcg tctcttcact gttccttgtg
     4261 gtggtcttca ttccatgctt ctctttgctc tgatcaaaag aacaacgggg gctttgtgtg
     4321 ggtgttctta cagggcggcc acaaatccta tttacctgtt ccaataattt ttgtccattg
     4381 aggtgagcct gtaacacagc atcattaaca agcaagtgaa agttcagcag cacgtgtcca
     4441 atgtcgtatg ttccatgcat tgcatccgag agttcttcca acgaccggat atatgcatgc
     4501 cagtgtggat taagctccgc catgtgcgcc aggcagcctc gcatgacatt gaggcagtat
     4561 cccatacaag gcttagtgag cgccaggcct tggcagtgcg ggcagtattg catcttcagg
     4621 agggctctgc tgcactcttt ggagaagtgc agatagtctg tggtgttgat gacttcaatg
     4681 cccagattga gtgcctgcag aaaagtgcgg ctgggcagca gggacctccc catctgtccc
     4741 attactcttt ggggaatatt accaaatgga ctcacatccc ggcgagccat ccggatgcat
     4801 tctgagtatt ccagggaact gtcagtcaca ccagggttaa tgaggtggtt gtagaccaga
     4861 ggaaaaagac tgtcaaaaaa tctgtttaca aattcttcag gattaacatc cgcaccaaat
     4921 aaatacagcc ccacatcagt gaagaactcc tgaaccgaag cagcagcctc caaggccatg
     4981 ttcctgtagg tactgcaaaa aagtatactg gtgtaatttt ctgcttgttt gatgagagtt
     5041 tcaagggttt cttgaaaagc agccgcattt cgagatatta gaaactttaa tgtagagctg
     5101 gacgtttgaa gaaactgctg catatcctgg cgagccgcaa tctgatatct ctcctccatc
     5161 ttcctggtgc aacatgtagg ctttttggat atgcaaacct gaagatcagg tcctgcccgc
     5221 ggcgaatccg gcagccccct gacagctccc agcagccgcc actggaaaag tttccgaact
     5281 tcttcgcagg tctgcacgcc ctcaccggtt tcagctacgc aacccatcag cagcacggag
     5341 agaaggctca cgagggagag cagcgcaggc atcccagggc tgggaaggat ccccatggtg
     5401 gcaagcttga attcggcctg agacggccgc aattctttgc caaaatgatg agacagcaca
     5461 ataaccagca cgttgcccag gagctgtagg aaaaagaaga aggcatgaac atggttagca
     5521 gaggctctag agccgccggt cacacgccag aagccgaacc ccgccctgcc ccgtcccccc
     5581 cgaaggcagc cgtccccccg cggacagccc cgaggctgga gagggagaag gggacggcgg
     5641 cgcggcgacg cacgaaggcc ctccccgccc atttccttcc tgccggcgcc gcaccgcttc
     5701 gccccgcgcc cgctagaggg ggtgcggcgg cgcctcccag atttcggct
//
