LOCUS       sequence-410396-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expression of human lactadherin in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  TITLE     Mariotti lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: psd44-Lactadherin46
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1575..1692
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(1704..1724)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1709..2038
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1889..2024
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2048..2647
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2048..2067)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2544..2564
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        2746..3256
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3175..3193
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    4527..5115
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4580..4600)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4998..5009)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5202..5435
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5507..5628
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5544..5563)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5598..5617
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5668..5803
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5823..5842)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5824..5842)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5852..5869)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5852..5868)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5861..5883)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6010..6465
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6097..6116)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6307..6328
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6491..6595
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6596..6664,6665..7456)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6814..6833)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7627..8215
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8116..8135
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8369..8386
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8503..8524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8539..8544,8545..8562,8563..8569)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     mobile_element  8570..9337
                     /mobile_element_type="insertion sequence:IS1"
                     /label=IS1
                     /note="prokaryotic transposable element"
     protein_bind    9354..9370
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9359..9381
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9378..9394
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9378..9394
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9460..9686
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     1621 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     1681 attcaaaatt ttatcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     1741 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     1801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1861 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     1921 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     1981 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     2041 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     2101 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     2161 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     2221 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac
     2281 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     2341 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     2401 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     2461 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     2521 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     2581 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     2641 tgcctgacgc ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc
     2701 atcgatcacg agactagcct cgagaagctt gatatcgaat tccacggggt tggggttgcg
     2761 ccttttccaa ggcagccctg ggtttgcgca gggacgcggc tgctctgggc gtggttccgg
     2821 gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc acgtccgttc gcagcgtcac
     2881 ccggatcttc gccgctaccc ttgtgggccc cccggcgacg cttcctgctc cgcccctaag
     2941 tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga caaacggaag ccgcacgtct
     3001 cactagtacc ctcgcagacg gacagcgcca gggagcaatg gcagcgcgcc gaccgcgatg
     3061 ggctgtggcc aatagcggct gctcagcagg gcgcgccgag agcagcggcc gggaaggggc
     3121 ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg ttcctgcccg cgcggtgttc
     3181 cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc tccctcgttg accgaatcac
     3241 cgacctctct ccccaggggg atccaccggt ggatccgttt aaacatatgc tagctgcagt
     3301 ctagatacgt acccgggtgt acaaaaaagc aggcaccatg ccgcgccccc gcctgctggc
     3361 cgcgctgtgc ggcgcgctgc tctgcgcccc cagcctcctc gtcgccctgg atatctgttc
     3421 caaaaacccc tgccacaacg gtggtttatg cgaggagatt tcccaagaag tgcgaggaga
     3481 tgtcttcccc tcgtacacct gcacgtgcct taagggctac gcgggcaacc actgtgagac
     3541 gaaatgtgtc gagccactgg gcatggagaa tgggaacatt gccaactcac agatcgccgc
     3601 ctcatctgtg cgtgtgacct tcttgggttt gcagcattgg gtcccggagc tggcccgcct
     3661 gaaccgcgca ggcatggtca atgcctggac acccagcagc aatgacgata acccctggat
     3721 ccaggtgaac ctgctgcgga ggatgtgggt aacaggtgtg gtgacgcagg gtgccagccg
     3781 cttggccagt catgagtacc tgaaggcctt caaggtggcc tacagcctta atggacacga
     3841 attcgatttc atccatgatg ttaataaaaa acacaaggag tttgtgggta actggaacaa
     3901 aaacgcggtg catgtcaacc tgtttgagac ccctgtggag gctcagtacg tgagattgta
     3961 ccccacgagc tgccacacgg cctgcactct gcgctttgag ctactgggct gtgagctgaa
     4021 cggatgcgcc aatcccctgg gcctgaagaa taacagcatc cctgacaagc agatcacggc
     4081 ctccagcagc tacaagacct ggggcttgca tctcttcagc tggaacccct cctatgcacg
     4141 gctggacaag cagggcaact tcaacgcctg ggttgcgggg agctacggta acgatcagtg
     4201 gctgcaggtg gacctgggct cctcgaagga ggtgacaggc atcatcaccc agggggcccg
     4261 taactttggc tctgtccagt ttgtggcatc ctacaaggtt gcctacagta atgacagtgc
     4321 gaactggact gagtaccagg accccaggac tggcagcagt aagatcttcc ctggcaactg
     4381 ggacaaccac tcccacaaga agaacttgtt tgagacgccc atcctggctc gctatgtgcg
     4441 catcctgcct gtagcctggc acaaccgcat cgccctgcgc ctggagctgc tgggctgtta
     4501 gaacccagct ttcttgtaca gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     4561 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4621 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4681 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4741 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4801 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4861 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4921 agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     4981 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5041 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5101 gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga cttacaaggc
     5161 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5221 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5281 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5341 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5401 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     5461 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     5521 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     5581 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     5641 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     5701 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     5761 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     5821 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     5881 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     5941 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6001 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6061 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6121 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6181 tccgatttag tgcattacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6241 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6301 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6361 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6421 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6481 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6541 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6601 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6661 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     6721 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     6781 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     6841 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     6901 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     6961 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7021 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7081 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7141 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7201 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7261 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7321 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7381 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7441 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7501 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7561 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7621 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7681 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7741 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7801 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7861 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7921 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7981 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8041 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8101 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8161 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8221 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8281 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8341 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8401 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8461 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8521 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgg gtaatgactc
     8581 caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca tgcagctcca
     8641 ccgattttga gaacgacagc gacttccgtc ccagccgtgc caggtgctgc ctcagattca
     8701 ggttatgccg ctcaattcgc tgcgtatatc gcttgctgat tacgtgcagc tttcccttca
     8761 ggcgggattc atacagcggc cagccatccg tcatccatat caccacgtca aagggtgaca
     8821 gcaggctcat aagacgcccc agcgtcgcca tagtgcgttc accgaatacg tgcgcaacaa
     8881 ccgtcttccg gagactgtca tacgcgtaaa acagccagcg ctggcgcgat ttagccccga
     8941 catagcccca ctgttcgtcc atttccgcgc agacgatgac gtcactgccc ggctgtatgc
     9001 gcgaggttac cgactgcggc ctgagttttt taagtgacgt aaaatcgtgt tgaggccaac
     9061 gcccataatg cgggctgttg cccggcatcc aacgccattc atggccatat caatgatttt
     9121 ctggtgcgta ccgggttgag aagcggtgta agtgaactgc agttgccatg ttttacggca
     9181 gtgagagcag agatagcgct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc
     9241 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca
     9301 ccatcataca ctaaatcagt aagttggcag catcacccgt atgttgtgtg gaattgtgag
     9361 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcgtttaa
     9421 actcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     9481 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     9541 accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     9601 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     9661 ttaagtgcct agctcgatac ataaac
//
