LOCUS       sequence-410409-        5631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  QPRT protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5631)
  TITLE     TREAT-AD plasmids Jun 2023
REFERENCE   2  (bases 1 to 5631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: QPRTA-c002
FEATURES             Location/Qualifiers
     source          1..5631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(512..517,518..534,535..540))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        892..983
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     908..925
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             1920..1940
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1941..1958
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2109..2243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2146..2165)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2200..2219
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  2272..2437
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      2621..3075
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2708..2727)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2917..2938
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3101..3205
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3206..3274,3275..4066)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3424..3443)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4237..4825
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4726..4745
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4979..4996
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  5130..5354
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(join(5421..5631,1..323))
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
ORIGIN
        1 atatagatct cactacgcgg ctgctcaaac ttgggcagaa cgtaagccgc gagagcgcca
       61 acaaccgctt cttggtcgaa ggcagcaagc gcgatgaatg tcttactacg gagcaagttc
      121 ccgaggtaat cggagtccgg ctgatgttgg gagtaggtgg ctacgtctcc gaactcacga
      181 ccgaaaagat caagagcagc ccgcatggat ttgacttggt cagggccgag cctacatgtg
      241 cgaatgatgc ccatacttga gccacctaac tttgttttag ggcgactgcc ctgctgcgta
      301 acatcgttgc tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc
      361 gtaacgcgct tgctgcttgg atgcccgagg catagactgt acaaaaaaac agtcataaca
      421 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
      481 gttgcgtgag cgcatacgct acttgcatta cagtttacga accgaacagg cttatgtcaa
      541 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
      601 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
      661 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
      721 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
      781 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagga
      841 ctctagctat agttctagtg gttggctacg tatactccgg aatattaata gatcatggag
      901 ataattaaaa tgataaccat ctcgcaaata aataagtatt ttactgtttt cgtaacagtt
      961 ttgtaataaa aaaacctata aatattccgg attattcata ccgtcccacc atcgggcgcg
     1021 gatccatgga tgctgaggga ctggctttac ttttacctcc agttacgctg gcagcgctag
     1081 tcgactcatg gcttcgtgaa gattgccctg ggctcaacta tgcggcgtta gttagcggtg
     1141 cgggcccttc tcaagcagcg ctttgggcca agagccccgg tgttctcgca gggcaaccgt
     1201 tcttcgatgc gatcttcaca cagttgaatt gtcaggtaag ttggttcctg ccagaaggca
     1261 gtaagctcgt ccccgtggcc cgagtagcgg aagtcagggg acccgctcac tgccttctcc
     1321 ttggcgaaag ggtcgcttta aatactttgg cccgatgttc tggtatagct tcggccgctg
     1381 cagccgcagt ggaagctgca cgtggggctg ggtggacagg gcacgttgct ggaaccagaa
     1441 agactacccc tgggttcaga ctagtagaga aatacggtct attggtaggc ggtgcggcgt
     1501 cacaccgcta cgatctggga ggtctggtaa tggtgaaaga caatcatgta gttgcagccg
     1561 gaggtgtcga gaaagcagtg cgggccgcac ggcaagcagc agactttacg ctgaaagttg
     1621 aagtggagtg ctcgagccta caagaggccg tgcaagctgc tgaggcgggc gccgaccttg
     1681 tactgttgga taactttaag ccggaggagt tacatccgac tgcaacggtg ctcaaggccc
     1741 agtttccatc cgtcgcggtt gaagcctctg gcggcataac attggataac ctccctcagt
     1801 tctgtggacc ccatattgac gtcatttcca tgggaatgtt aacccaggcc gcaccggctt
     1861 tggacttttc gctaaagcta tttgctaaag aagtggcgcc ggtcccaaaa atccatgcag
     1921 agaacctcta cttccaatcg caccatcatc accatcactg actcgaggca tgcggtacca
     1981 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     2041 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     2101 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     2161 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     2221 atgtatctta tcatgtctgg atctgatcac tgcttgagcc taggagatcc gaaccagata
     2281 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     2341 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     2401 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
     2461 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctactttt
     2521 tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac
     2581 tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     2641 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2701 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2761 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2821 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2881 tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc caaactggaa
     2941 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     3001 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     3061 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     3121 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     3181 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     3241 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     3301 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     3361 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     3421 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     3481 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     3541 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     3601 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     3661 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3721 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3781 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3841 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3901 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3961 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     4021 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     4081 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     4141 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     4201 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     4261 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     4321 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     4381 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     4441 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     4501 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     4561 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     4621 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     4681 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4741 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4801 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4861 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4921 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4981 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     5041 gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg ttacggttga
     5101 gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac
     5161 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
     5221 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
     5281 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
     5341 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     5401 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     5461 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     5521 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
     5581 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata g
//
