LOCUS       sequence-410506-        5934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Luciferase experiment for TAL1 TSS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Sharma A, Mistriel-Zerbib S, Najar RA, Engal E, Bentata M, Taqatqa 
            N, Dahan S, Cohen K, Jaffe-Herman S, Geminder O, Baker M, Nevo Y, 
            Plaschkes I, Kay G, Drier Y, Berger M, Salton M
  TITLE     Isoforms of the TAL1 transcription factor have different roles in 
            hematopoiesis and cell growth.
  JOURNAL   PLoS Biol. 2023 Jun 28;21(6):e3002175. doi: 
            10.1371/journal.pbio.3002175.
  PUBMED    37379322
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2023-06-28"; volume: "21(6):e3002175. doi"; pages: " 
            10.1371/journal.pbio.3002175"
COMMENT     Sequence Label: pGL4.23_TSS1
FEATURES             Location/Qualifiers
     source          1..5934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1511..1542
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      1569..1578
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1792..3444
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3488..3609
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3499..3518)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3553..3572
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3857..3874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4028..4616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4108..4127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4816..5607,5608..5676))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5781..5829
                     /note="synthetic polyadenylation signal"
     misc_feature    5843..5934
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5883..5902
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct tgagcaggat agtggcatga ccagctctat cttctttttt
       61 tttttttttg agatggagtc tcactctgtt gcccaggcta gagtgcagtg gcacgatctc
      121 ggctcaccac aacctccacc tcctgggttc aagtgattct tctgcctcag cttcctgagt
      181 agctgggatt aaaggcgtgc gccaccacac ccggctcatt ttgtattttt agtacagatg
      241 gggtttctcc atgttggtca ggctggtctc gactcccgac ctcaggtgat ccacctgcct
      301 cggtctccca aagtgctggg attacaggcg tgagccaccg tgcccggcct gaccagctct
      361 atctttaaaa gatcactcca ctgtgatgtg gagaaggggt tgcagggcaa tggtggacac
      421 aggaagggct gataagaagc aactgtgata gtccagacac ctgcctctgg gagcctaaaa
      481 cactccacat aggtgggaag gcttcaaaag aggcctgggg atgaagctgt tgccctccag
      541 cctgccgcgc ttcccgggga cctgcaaacg tccatccttt cctcggtcga ttcctcgtcc
      601 agcctgtggt ccttggggct ctgatttatt cagcttggga accaggcagc gagtctcatg
      661 tctgccccag gcctcaaaat agattatttt ttttattagc tgtcccttgt taactcataa
      721 ttgcaatcat gtaacattta atagaggcac aaagcgatcc ccatcactgc tccacaatca
      781 ttcattagct aacaagacag agcagctcat aaaaaaaaag ccgttaaaaa aattccgggg
      841 aaatggaaag caggaggtga tgcaagccct ggttaacaaa ggctgagggt tggggggagg
      901 catgagaggg tgtgagtgga ataacccaag cctgataagc cacaaagcag cgcctgctgc
      961 tccctcctcc ctctgccgcc tgagtcagag aagccgggat gtgttcaaaa tcaagcaatg
     1021 taattagcag gttgcatcat gcctctcgat tataaattac aatggccaca aagagggtgg
     1081 atggaggagc agggattaga tggggtaacc caatcccagt gccgggaagg gggacagagg
     1141 gcccgggagc tgccccccgc cactaggagc cactcggagt ggcctctgtc tactgtgtct
     1201 agggacagca acaaggccta ctcaatacct gctttgtgta gcctgggata atgatttaca
     1261 aactcaagga aatcaggagg tgtgaaaaga agatagccct cttaacccag ccattgtcta
     1321 ccctctgctg gaatgtccct cagctccacc cacctggtgc acctgtgcac acggatgctg
     1381 tttgaggatg gtccacgtga ggagccctca taggggctgg actgataaga tctaaaaggg
     1441 ttgctgggct cgaggagctc gctagcctcg aggatatcaa gatctggcct cggcggccaa
     1501 gcttagacac tagagggtat ataatggaag ctcgacttcc agcttggcaa tccggtactg
     1561 ttggtaaagc caccatggcg ctcaggaccg ggcctcaaaa tggccacacg cgtacccccg
     1621 tagcggaaaa acatcgccca ggaccacacc gcagcgtaac tgcaggcctc tcagcgaaaa
     1681 agggggaaag caaagacccg ggtgtgcatc ctcttcctcg gcttccgccc ctttccggcg
     1741 gagtggagat cctattcaga ggggccggtc tctctaaata tgccccagcc catggaagat
     1801 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc
     1861 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc
     1921 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg
     1981 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag
     2041 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc
     2101 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc
     2161 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta
     2221 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc
     2281 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc
     2341 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga
     2401 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc
     2461 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt
     2521 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg
     2581 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa
     2641 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag
     2701 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta
     2761 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca
     2821 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc
     2881 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt
     2941 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac
     3001 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc
     3061 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa
     3121 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac
     3181 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca
     3241 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc
     3301 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct
     3361 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag
     3421 aagggcggca agatcgccgt gtaataattc tagagtcggg gcggccggcc gcttcgagca
     3481 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     3541 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     3601 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     3661 gaggtttttt aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatccgtcg
     3721 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
     3781 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
     3841 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     4021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     4081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     4141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     4201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     4261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     4321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     4381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4441 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagcggc cgcaaatgct
     4801 aaaccactgc agtggttacc agtgcttgat cagtgaggca ccgatctcag cgatctgcct
     4861 atttcgttcg tccatagtgg cctgactccc cgtcgtgtag atcactacga ttcgtgaggg
     4921 cttaccatca ggccccagcg cagcaatgat gccgcgagag ccgcgttcac cggcccccga
     4981 tttgtcagca atgaaccagc cagcagggag ggccgagcga agaagtggtc ctgctacttt
     5041 gtccgcctcc atccagtcta tgagctgctg tcgtgatgct agagtaagaa gttcgccagt
     5101 gagtagtttc cgaagagttg tggccattgc tactggcatc gtggtatcac gctcgtcgtt
     5161 cggtatggct tcgttcaact ctggttccca gcggtcaagc cgggtcacat gatcacccat
     5221 attatgaaga aatgcagtca gctccttagg gcctccgatc gttgtcagaa gtaagttggc
     5281 cgcggtgttg tcgctcatgg taatggcagc actacacaat tctcttaccg tcatgccatc
     5341 cgtaagatgc ttttccgtga ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat
     5401 acggcgacca agctgctctt gcccggcgtc tatacgggac aacaccgcgc cacatagcag
     5461 tactttgaaa gtgctcatca tcgggaatcg ttcttcgggg cggaaagact caaggatctt
     5521 gccgctattg agatccagtt cgatatagcc cactcttgca cccagttgat cttcagcatc
     5581 ttttactttc accagcgttt cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa
     5641 gggaatgagt gcgacacgaa aatgttggat gctcatactc gtcctttttc aatattattg
     5701 aagcatttat cagggttact agtacgtctc tcaaggataa gtaagtaata ttaaggtacg
     5761 ggaggtattg gacaggccgc aataaaatat ctttattttc attacatctg tgtgttggtt
     5821 ttttgtgtga atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca
     5881 aactagcaaa ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctct
//
