LOCUS       sequence-410530-        6851 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDefence6:Eluc (defence-induced promoter with green-shifted 
            luciferase expression cassette).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6851)
  TITLE     Schwessinger Lab Plasmids 2023
REFERENCE   2  (bases 1 to 6851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSW006
FEATURES             Location/Qualifiers
     source          1..6851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             2085..2093
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      2254..2506
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2554..2578
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(2734..3882)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     rep_origin      complement(4218..4806)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4298..4317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4977..5768,5769..5837))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5600..5619
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5838..5942)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6029..6660
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    6752..6776
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tctgtgaaga caatgccgaa ttcggatccg gagaaatcct tagccgtgta gcaccgactg
       61 tcggaccgac gcggttaagc taggtagcat gcaaacatac tcggtcgtgt tcgagtagtc
      121 tccattgcta ctggagatca agccgcgtgc gtgtcattat ccatctcaag aaaacgcgcg
      181 acggcgattg atggagccac actacgaaag cgaaatatat ttgagatcgg tccgtatcga
      241 tgcggtatga acaaattgaa aaaacaaatc tgtgacgtga gtactcaagc aaacacaaaa
      301 aacatcttat atttgtgatc agaagggtag ctaaaagcac acgtcgaaaa agttgagaac
      361 gcgtaagagg aacgcgcgta ggtacgtgcg agtcagctgc atatgctagc tccgcccgta
      421 catgatcttc tccatcctat ataaagagac atgcatgccc tcatgtcaag cacatatcac
      481 aaacacacat acaaacacag atcctcatcc tccattcctc cggtataacc gtcgatcaca
      541 caaagcaagg cacgagtgcg tacgcacgca cacgcacgta ctccgttgag agctcggaag
      601 gctaattggt gaatggagag agagaagaac gtggtgtacg gccccgagcc caagcaccct
      661 ctgggcaact tcaccgccgg cgagatgctg tacaacgctc tgcacaagca ctcccacatc
      721 ccccaggcca tcctggacgt gatgggcaac gagtcccttt cctaccagga gttcttcgac
      781 actactgtga agctgggcca gagcctccag aactgtggct acaagatgaa cgatgtcgtg
      841 tcgatctgtg cagagaacaa caagagattc ttcatcccca tcatctccgc ctggtacatc
      901 ggcatggtgg tggcccctgt gaacgaggac tatatcccag acgagctgtg taaagtgacc
      961 ggcatctcca agccgatcct ggtctttacc actaggaaga tcctgcctaa ggttttggag
     1021 gttaaagaca gaaccaacta cataaagagg atcatcatac tggactctga agagaacctg
     1081 ctgggctgcg agagcctgca caacttcatg tccaggtact ccgacaacaa cctccaaaca
     1141 ttcaagcctc tgcactacga ccctgtggac caggtagccg ccatcctgtg ctcctccggc
     1201 acaaccggcc tgcctaaagg cgtgatgcag acccacagga acatctgtgt gagactcaca
     1261 cacgcatctg accccagagt gggtacacaa ctcatccccg gcgtatccgt gctggcctac
     1321 ctgccattct tccacgcctt cggcttcagt atcaacctgg gctatttcat ggtgggcctg
     1381 agagtggtga tgctccgaag gtttaaccag gaggtgttcc tgaaggccat ccaggactac
     1441 gaggtgagga gcgtgatcaa cgttccctcc acaatcctgt tcctgtccaa gagccctctg
     1501 gtggacaagt acgacctatc caccctggcg gagctgtgct gtggagccgc tcctctggcg
     1561 aaggaggtgg ccgagatcgc cgtgaagagg ctgaacctgc cagggatacg gtgtggctac
     1621 ggtctaacag agtctacctc cgccaacatc catactctgc acaacgagtt caagtccggc
     1681 tccctgggca aggtgacacc ttacatggcc gccaagatca tcgacaggaa caccggcgag
     1741 gccctgggtc caaaccaggt gggcgagctg tgcatctggg gacctatggt aacaaaaggc
     1801 tatgtgaaca acccacaggc tactaaggag gccatcgacg acgacggctg gctgcactct
     1861 ggcgacttcg gctactacga cgaggacgag tatttctaca tcgtggaccg gtacaaggag
     1921 ctgatcaaat acaagggcta tcaggtcgcc cctgtggagc tggaggagat cctccttcag
     1981 cacccaggca tcagggacgt ggccgtcgtg ggtatccctg acatcgaggc cggcgagctg
     2041 ccagccggct tcgtggtgaa gcagcccggc gcccaactca ccgctaagga ggtgtacgac
     2101 ttcctggccc agagggtgtc tcactccaag tacctgaggg gcggcgtaag gttcgtggac
     2161 tctatcccca ggaacgtgac aggcaagatt agtcgaaaag agctgaggga ggccctgatg
     2221 gagaaggctt ctaagctgta agcttgtcaa gcagatcgtt caaacatttg gcaataaagt
     2281 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     2341 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2401 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2461 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgacg ctgcaattgt
     2521 cttctgcacg aagtggttta aactatcagt gtttgacagg atatattggc gggtaaacct
     2581 aagagaaaag agcgtttatt agaataatcg gatatttaaa agggcgtgaa aaggtttatc
     2641 cgttcgtcca tttgtatgtg catgccaacc acagggttcc ccagatcagg cgctggctgc
     2701 tgaaccccca gccggaactg accccacaag gccctagcgt ttgcaatgca ccaggtcatc
     2761 attgacccag gcgtgttcca ccaggccgct gcctcgcaac tcttcgcagg cttcgccgac
     2821 ctgctcgcgc cacttcttca cgcgggtgga atccgatccg cacatgaggc ggaaggtttc
     2881 cagcttgagc gggtacggct cccggtgcga gctgaaatag tcgaacatcc gtcgggccgt
     2941 cggcgacagc ttgcggtact tctcccatat gaatttcgtg tagtggtcgc cagcaaacag
     3001 cacgacgatt tcctcgtcga tcaggacctg gcaacgggac gttttcttgc cacggtccag
     3061 gacgcggaag cggtgcagca gcgacaccga ttccaggtgc ccaacgcggt cggacgtgaa
     3121 gcccatcgcc gtcgcctgta ggcgcgacag gcattcctcg gccttcgtgt aataccggcc
     3181 attgatcgac cagcccaggt cctggcaaag ctcgtagaac gtgaaggtga tcggctcgcc
     3241 gataggggtg cgcttcgcgt actccaacac ctgctgccac accagttcgt catcgtcggc
     3301 ccgcagctcg acgccggtgt aggtgatctt cacgtccttg ttgacgtgga aaatgacctt
     3361 gttttgcagc gcctcgcgcg ggattttctt gttgcgcgtg gtgaacaggg cagagcgggc
     3421 cgtgtcgttt ggcatcgctc gcatcgtgtc cggccacggc gcaatatcga acaaggaaag
     3481 ctgcatttcc ttgatctgct gcttcgtgtg tttcagcaac gcggcctgct tggcctcgct
     3541 gacctgtttt gccaggtcct cgccggcggt ttttcgcttc ttggtcgtca tagttcctcg
     3601 cgtgtcgatg gtcatcgact tcgccaaacc tgccgcctcc tgttcaagac gacgcgaacg
     3661 ctccacggcg gccgatggcg cgggcagggc agggggagcc agttgcacgc tgtcgcgctc
     3721 gatcttggcc gtagcttgct ggaccatcga gccgacggac tggaaggttt cgcggggcgc
     3781 acgcatgacg gtgcggcttg cgatggtttc ggcatcctcg gcggaaaacc ccgcgtcgat
     3841 cagttcttgc ctgtatgcct tccggtcaaa cgtccgattc attcaccctc cttgcgggat
     3901 tgccccgact cacgccgggg caatgtgccc ttattcctga tttgacccgc ctggtgcctt
     3961 ggtgtccaga taatccacct tatcggcaat gaagtcggtc ccgtagaccg tctggccgtc
     4021 cttctcgtac ttggtattcc gaatcttgcc ctgcacgaat accagcgacc ccttgcccaa
     4081 atacttgccg tgggcctcgg cctgagagcc aaaacacttg atgcggaaga agtcggtgcg
     4141 ctcctgcttg tcgccggcat cgttgcgcca catctaggat ctgccaggaa ccgtaaaaag
     4201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     4261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     4321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     4381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     4441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     4501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     4561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     4621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     4681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     4741 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4801 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4861 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4921 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4981 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     5041 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     5101 gctgcaatga taccgcgaga accacgctca ccggctccag atttatcagc aataaaccag
     5161 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     5221 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     5281 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     5341 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     5401 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     5461 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     5521 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     5581 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     5641 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     5701 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     5761 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5821 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcaaggttat
     5881 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5941 cgcacgaatt ggccagcgct gccatttttg gggtgaggcc gttcgcggcc gaggggcgca
     6001 gcccctgggg ggatgggagg cccgcgttag cgggccggga gggttcgaga agggggggca
     6061 ccccccttcg gcgtgcgcgg tcacgcgcac agggcgcagc cctggttaaa aacaaggttt
     6121 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
     6181 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
     6241 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
     6301 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc
     6361 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc
     6421 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg
     6481 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgagg
     6541 tatccacaac gccggcggcc gcggtgtctc gcacacggct tcgacggcgt ttctggcgcg
     6601 tttgcagggc catagacggc cgccagccca gcggcgaggg caaccagccc ggtgagcgtc
     6661 gcaaaggaga tcctgatctg actgatgggc tgcctgtatc gagtggtgat tttgtgccga
     6721 gctgccggtc ggggagctgt tggctggctg gtggcaggat atattgtggt gtaaacaaat
     6781 tgacgcttag acaacttaat aacacattgc ggacgttttt aatgtactgg ggtggatgca
     6841 gtgggcccca c
//
